While Chinese dance is a popular dance genre among Chinese teenagers and adults, little is known regarding the prevalence of dance-related injuries or factors associated with such injuries among ...Chinese dance practitioners. The current cross-sectional study aimed to determine the prevalence of dance-related injuries and their associated risk factors among young Chinese dance practitioners in Hong Kong. Online surveys were distributed to dancers through local dance associations, while paper-based surveys were distributed to young Chinese dance performers during the 54
th
School Dance Festival in Hong Kong. Self-reported hours of dancing, injuries in the last 12 months, injury sites, and related factors were collected. The injury rate, 12-month prevalence of dance-related injuries were determined. Risk factors for common dance injuries were analyzed using separate multivariate regression models. A total of 175 children (aged 10–14 years) and 118 young (aged 15–24 years) Chinese dance practitioners provided their dance injury information. Young dancers had a significantly higher injury rate (6.5 injuries vs 4.6 injuries/1000 dance hours) and 12-month prevalence (52.5% vs 19.4%) than their child counterparts. The most commonly injured sites were the knee (children:7.4%; young:15.3%), lower back (children: 4.6%; young: 9.5%), and ankles (children: 5.1%; young: 16.9%). Age was a significant independent risk factor for dance-related injuries to the upper back, lower back, and pelvis/buttock (odds ratios ranging from 1.2 to 1.3/additional years). Additionally, height was a significant independent risk factor for lower limb injury (odds ratios ranging from 1.0–1.1/additional centimeter). Collectively, young Chinese dance practitioners are more vulnerable to dance-related injuries than are child dancers. Older age increases the risk of trunk injuries, whereas taller dancers are more likely to sustain lower-limb injuries. Future research should determine the mechanisms underlying dance-related injuries among these dancers.
The radio technique is a promising method for detection of cosmic-ray air showers of energies around 100PeV and higher with an array of radio antennas. Since the amplitude of the radio signal can be ...measured absolutely and increases with the shower energy, radio measurements can be used to determine the air-shower energy on an absolute scale. We show that calibrated measurements of radio detectors operated in coincidence with host experiments measuring air showers based on other techniques can be used for comparing the energy scales of these host experiments. Using two approaches, first via direct amplitude measurements, and second via comparison of measurements with air shower simulations, we compare the energy scales of the air-shower experiments Tunka-133 and KASCADE-Grande, using their radio extensions, Tunka-Rex and LOPES, respectively. Due to the consistent amplitude calibration for Tunka-Rex and LOPES achieved by using the same reference source, this comparison reaches an accuracy of approximately 10% – limited by some shortcomings of LOPES, which was a prototype experiment for the digital radio technique for air showers. In particular we show that the energy scales of cosmic-ray measurements by the independently calibrated experiments KASCADE-Grande and Tunka-133 are consistent with each other on this level.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
LOPES was a digital antenna array detecting the radio emission of cosmic-ray air showers. The calibration of the absolute amplitude scale of the measurements was done using an external, commercial ...reference source, which emits a frequency comb with defined amplitudes. Recently, we obtained improved reference values by the manufacturer of the reference source, which significantly changed the absolute calibration of LOPES. We reanalyzed previously published LOPES measurements, studying the impact of the changed calibration. The main effect is an overall decrease of the LOPES amplitude scale by a factor of 2.6 ± 0.2, affecting all previously published values for measurements of the electric-field strength. This results in a major change in the conclusion of the paper ‘Comparing LOPES measurements of air-shower radio emission with REAS 3.11 and CoREAS simulations’ published by Apel et al. (2013) : With the revised calibration, LOPES measurements now are compatible with CoREAS simulations, but in tension with REAS 3.11 simulations. Since CoREAS is the latest version of the simulation code incorporating the current state of knowledge on the radio emission of air showers, this new result indicates that the absolute amplitude prediction of current simulations now is in agreement with experimental data.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK, ZRSKP
The purpose of this work was to develop high‐resolution cardiac magnetic resonance imaging techniques for the in vivo mouse model for quantification of myocardial function and mass. Eight male mice ...were investigated on a 7‐Tesla MRI scanner. High‐quality images in multiple short axis slices (in‐plane resolution 117 μm2, slice thickness 1 mm) were acquired with an ECG‐gated cine sequence. Left ventricular end‐diastolic and end‐systolic volumes and mass were calculated from segmented slice volumes. There was precise agreement of left ventricular mass determined ex vivo and by MRI. lntraobserver (5%) and interobserver (5%) variability of in vivo MR measurements were low.
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BFBNIB, FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SBCE, SBMB, UL, UM, UPUK
The aim of this study was to test the feasibility of cine magnetic resonance imaging (MRI) for assessment of the infarcted rat and mouse heart and to compare the results with established methods. ...These models have been proven to predict genesis and prevention of heart failure in patients. The value of cine MRI was tested in studies investigating interventions to change the course of the remodeling process. MRI was performed for determination of left ventricular (LV) volumes and mass, myocardial infarct (MI) size and cardiac output. LV wet weight was determined after MRI. Rats underwent conventional hemodynamic measurements for determination of cardiac output and LV volumes by electromagnetic flowmeter and pressure–volume curves. Infarct size was determined by histology. MRI-acquired MI-size (18.5±2%) was smaller than that found by histology (22.8±2.5%,
p<0.05) with close correlation (
r=0.97). There was agreement in LV mass between MRI and wet weight (
r=0.97,
p<0.05) and in the MRI- and flowmeter measurements of cardiac output (
r=0.80,
p<0.05). Volume by MRI differed from pressure–volume curves with good correlation (
r=0.96,
p<0.05). In a serial study of mice after coronary ligation, LV hypertrophy at 8 weeks was detected (Sham 105.1±7.9 mg, MI 144.4±11.7 mg,
p<0.05). Left ventricles were enlarged in infarcted mice (end-diastolic volume, week 8: Sham 63.5±4 μl, MI 94.2 μl,
p<0.05). In conclusion, cine MRI is a valuable diagnostic tool applicable to the rat and mouse model of MI. Being non-invasive and exact it offers new insights into the remodeling process after MI because serial measurements are possible. The technique was applied to study several interventions and proved its usefulness.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK
1 Department of Cardiology,
2 Institute of Biophysics, University of
Würzburg, 97080 Würzburg, Germany
Cardiovascular transgenic mouse models with an early
phenotype or even premature death require ...noninvasive imaging methods that allow for accurate visualization of cardiac morphology and function. Thus the purpose of our study was to assess the feasibility of magnetic resonance imaging (MRI) to characterize cardiac function and mass in newborn, juvenile, and adult mice. Forty-five C57bl/6 mice
from seven age groups (3 days to 4 mo after birth) were studied by MRI
under isoflurane anesthesia. Electrocardiogram-gated cine MRI was
performed with an in-plane resolution of (78-117
µm) 2 . Temporal resolution per cine frame was 8.6 ms. MRI
revealed cardiac anatomy in mice from all age groups with high temporal and spatial resolution. There was close correlation between MRI- and
autopsy-determined left ventricular (LV) mass ( r = 0.95, SE of
estimate = 9.5 mg). The increase of LV mass (range
9.6-101.3 mg), cardiac output (range 1.1-14.3 ml/min), and
stroke volume (range 3.2-40.2 µl) with age could be quantified
by MRI measurements. Ejection fraction and cardiac index did not change
with aging. However, LV mass index decreased with increasing age
( P < 0.01). High-resolution MRI allows for accurate in vivo
assessment of cardiac function in neonatal, juvenile, and adult mice.
This method should be useful when applied in transgenic mouse models.
magnetic resonance imaging; mouse heart; neonatal physiology; ventricular mass
ABSTRACT—The aim of this study was to assess the capability of MRI to characterize systolic and diastolic function in normal and chronically failing mouse hearts in vivo at rest and during inotropic ...stimulation. Applying an ECG-gated FLASH-cine sequence, MRI at 7 T was performed at rest and after administration of 1.5 μg/g IP dobutamine. There was a significant increase of heart rate, cardiac output, and ejection fraction and significant decrease of end-diastolic and end-systolic left ventricular (LV) volumes (P <0.01 each) in normal mice during inotropic stimulation. In mice with heart failure due to chronic myocardial infarction (MI), MRI at rest revealed gross LV dilatation. There was a significant decrease of LV ejection fraction in infarcted mice (29%) versus sham mice (58%). Mice with MI showed a significantly reduced maximum LV ejection rate (P <0.001) and LV filling rate (P <0.01) and no increase of LV dynamics during dobutamine action, indicating loss of contractile and relaxation reserve. In 4-month-old transgenic mice with cardiospecific overexpression of the β1-adrenergic receptor, which at this early stage do not show abnormalities of resting cardiac function, LV filling rate failed to increase after dobutamine stress (transgenic, 0.19±0.03 μL/ms; wild type, 0.36±0.01 μL/ms;P <0.01). Thus, MRI unmasked diastolic dysfunction during dobutamine stress. Dobutamine-stress MRI allows noninvasive assessment of systolic and diastolic components of heart failure. This study shows that MRI can demonstrate loss of inotropic and lusitropic response in mice with MI and can unmask diastolic dysfunction as an early sign of cardiac dysfunction in a transgenic mouse model of heart failure.
This study compared the ability of a variety of light sources and exposure modes to polymerize a dual-cured resin composite through ceramic discs of different thicknesses by depth of cure and Vickers ...microhardness (VHN). Ceramic specimens (360) (Empress 2 Ivoclar Vivadent, color 300, diameter 4 mm, height 1 or 2 mm) were prepared and inserted into steel molds according to ISO 4049, after which a dual-cured composite resin luting material (Variolink II Ivoclar Vivadent) with and without self-curing catalyst was placed. The light curing units used were either a conventional halogen curing unit (Elipar TriLight 3M/ESPE for 40 seconds), a high-power halogen curing unit (Astralis 10 Ivoclar Vivadent for 20 seconds), a plasma arc curing unit (Aurys Degré K for 10 seconds or 20 seconds) or different light emitting diode (LED) curing units (Elipar FreeLight I 3M/ESPE for 40 seconds, Elipar FreeLight II 3M/ESPE for 20 seconds, LuxOmax Akeda for 40 seconds, e-Light GC for 12 seconds or 40 seconds). Depth of cure under the ceramic discs was assessed according to ISO 4049, and VHN at 0.5 and 1.0 mm distance from the ceramic disc bottom was determined (ISO 6507-1). Medians and the 25th and 75th percentiles were determined for each group (n=10), and statistical analysis was performed using the Mann-Whitney-U-test (p < or = 0.05). The results showed that increasing ceramic disc thickness had a negative effect on the curing depth and hardness of all light curing units, with hardness decreasing dramatically under the 2-mm thick discs using LuxOmax, e-Light (12 seconds) or Aurys (10 seconds or 20 seconds). The use of a self-curing catalyst is recommended over the light-curable portion only, because it produced an equivalent or greater hardness and depth of cure with all light polymerization modes.
Creatine kinase (CK) is responsible for the transport of high-energy phosphates in excitable tissue and is of central importance in myocardial energy homeostasis. Significant changes in myocardial ...energetics have been reported in mice lacking the various CK isoenzymes. Our hypothesis was that ablation of CK isoenzymes leads to cardiac hypertrophy, impaired function, and aggravation of left ventricular remodeling post-myocardial infarction.
CK-deficient mice (CK KO) were examined by cardiac magnetic resonance imaging (MRI) to determine left ventricular volumes, ejection fraction, and mass: ten wild-type (WT), 6 mitochondrial CK KO (Mito-CK-/-), 10 cytosolic CK KO (M-CK-/-), and 10 mice with combined KO (M/Mito-CK-/-).
While ejection fraction was similar in all groups, there was significant LV dilatation with a approximately 30% increase in LV end-diastolic volumes in Mito-CK-/- and in M/Mito-CK-/-. Compared to WT, there was a striking 73% and 64% increase of LV mass in Mito-CK-/- and in M/Mito-CK-/- mice, respectively, but no significant increase of LV mass (+33%; p=n.s.) in M-CK-/-. Furthermore, significant re-expression of beta-MHC, a marker of myocardial hypertrophy, was found in all CK-deficient hearts. LV remodeling was investigated by MRI in hearts of 7 WT and 10 M/Mito-CK-/- mice 4 weeks postmyocardial infarction (MI). Four weeks post-LAD ligation (MI size approximately 32%), WT and M/Mito-CK-/- showed a similar degree of cardiac dysfunction, dilatation, and hypertrophy.
Mito-CK-/- and M/Mito-CK-/- mice show significant LV dilatation and marked LV hypertrophy, but LV remodeling post-MI is not aggravated. CK ablation leads to substantial adaptational changes in heart.
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