Although three-dimensional (3D) bioprinting technology has gained much attention in the field of tissue engineering, there are still several significant engineering challenges to overcome, including ...lack of bioink with biocompatibility and printability. Here, we show a bioink created from silk fibroin (SF) for digital light processing (DLP) 3D bioprinting in tissue engineering applications. The SF-based bioink (Sil-MA) was produced by a methacrylation process using glycidyl methacrylate (GMA) during the fabrication of SF solution. The mechanical and rheological properties of Sil-MA hydrogel proved to be outstanding in experimental testing and can be modulated by varying the Sil-MA contents. This Sil-MA bioink allowed us to build highly complex organ structures, including the heart, vessel, brain, trachea and ear with excellent structural stability and reliable biocompatibility. Sil-MA bioink is well-suited for use in DLP printing process and could be applied to tissue and organ engineering depending on the specific biological requirements.
BACKGROUND AND PURPOSEThe safety and efficacy of tirofiban during endovascular therapy in patients undergoing intravenous thrombolysis with recombinant IV tPA remain unclear. This study aimed to ...investigate the safety and efficacy of intra-arterial tirofiban use during endovascular therapy in patients treated with IV tPA. MATERIALS AND METHODSUsing a multicenter registry, we enrolled patients with acute ischemic stroke who underwent endovascular therapy. Safety outcomes included postprocedural parenchymal hematoma type 2 and/or thick subarachnoid hemorrhage, intraventricular hemorrhage, and 3-month mortality. Efficacy outcomes included the successful reperfusion rate, postprocedural reocclusion, and good outcomes at 3 months (mRS scores of 0-2). The tirofiban effect on the outcomes was evaluated using a multivariable analysis while adjusting for potential confounders. RESULTSAmong enrolled patients, we identified 314 patients with stroke (279 and 35 patients in the no tirofiban and tirofiban groups, respectively) due to an intracranial artery occlusion who underwent endovascular therapy with intravenous thrombolysis. A multivariable analysis revealed no association of intra-arterial tirofiban with postprocedural parenchymal hematoma type and/or thick subarachnoid hemorrhage (adjusted OR, 1.07; 95% CI, 0.20-4.10; P = .918), intraventricular hemorrhage (adjusted OR, 0.43; 95% CI, 0.02-2.85; P = .467), and 3-month mortality (adjusted OR, 0.38; 95% CI, 0.04-1.87; P = .299). Intra-arterial tirofiban was not associated with good outcome (adjusted OR, 2.22; 95% CI, 0.89 -6.12; P = .099). CONCLUSIONSUsing intra-arterial tirofiban during endovascular therapy after IV tPA could be safe.
Composite films with silver nanoparticles (AgNPs), obtained by reduction of AgNO3 using an environmentally friendly method (combined reduction of AgNO3 by trisodium citrate solution and heating), and ...agar as a polymer matrix and a capping agent were prepared by solvent casting method. The surface color of the resulting agar/AgNPs films exhibited the characteristic plasmonic effect of the silver nanoparticles, and the characteristic properties of the composite films were greatly influenced by changing the concentration of AgNPs added. Significant increase in water vapor barrier properties and surface hydrophobicity were observed with increase in the concentration of AgNPs without reduction in the mechanical strength. In addition, the agar/AgNPs films loaded with more than 1 wt% of silver nanoparticles exhibited strong antimicrobial activity against both Gram-positive (Listeria monocytogenes) and Gram-negative (Escherichia coli O157:H7) bacterial pathogens.
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•Agar/silver nanoparticle composite films were prepared by reduction of AgNO3.•Film properties were dependent on the concentration of silver nanoparticles.•The composite film exhibited improved water vapor, gas barrier and mechanical properties.•The composite film with 1 and 2% of AgNPs showed strong antimicrobial activity.•The film can be potentially used as active packaging systems for preserving food quality and extending the shelf life.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK
Pristine and Ce doped TiO2 nanoparticles were fabricated for toxic pollutants removal from wastewater. Pristine, 2% Ce and 4% Ce doped TiO2 photocatalysts were produced via hydrothermal route. 4% Ce ...doped TiO2 exhibited 2.41 eV bandgap which is smaller than pure TiO2. The morphology was also investigated and it was established that doping of Ce ions enhanced the surface roughness and reduced the particle size. The surface area was characterized through BET analysis and 4% Ce–TiO2 possess higher surface with large pore diameter which helped the photocatalytic activity. The prepared photocatalysts were investigated on reduction of pollutants from wastewater under visible light. Higher efficiency was obtained for 4% Ce–TiO2 photocatalyst for both model pollutants. The “k” value possessed was also higher for the doped TiO2 catalyst. These analysis reports the optimum level of ceria doping to enhance morphology, surface area and it increased activity than bare TiO2. 4% Ce–TiO2 will be the potential candidate for efficient wastewater management. The 4% Ce doped TiO2 photocatalyst provided 77% and 88% on reducing MB and RhB dyes. The dopant has developed higher surface area, morphology and good recombination rate which reduced the toxic pollutants and changed the wastewater to reuse.
•4% Ce–TiO2 possess higher surface with large pore diameter.•4% Ce–TiO2 explored higher efficiency for both model pollutants.•The “k” value possessed was also higher for the doped TiO2 catalyst.•4% Ce–TiO2 will be the potential candidate for efficient wastewater management.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Objective To explore the correlation between microRNA-34a (miR-34a) and Notch1, and evaluate the influence of miR-34a overexpression on proliferation of bladder cancer cell line T24. Methods ...Bioinformatics software were used for predicting the binding site of miR-34a and Notch1, and luciferase assay was performed for confirming the direct regulatory relationship between them. miR-34a plasmid was transfected into bladder cancer cell line T24. Notch1 expression was detected by quantitative real-time polymerase chain reaction and Western blotting. Cell proliferation was assayed by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay. Apoptosis and cell cycle were assessed by flow cytometry. Results Luciferase assays showed that miR-34a transfection significantly down-regulated the normalized Notch1 3'UTR luciferase activity (P=0.006). Transfection of miR-34a reduced the RNA and protein levels of Notch1. Cell proliferation assay revealed that miR-34a transfection
A supercapacitor is an efficient energy storage system that acts as an excellent booster to deliver high power density required for batteries and fuel cells. Recently, composite material–based ...supercapacitors have attracted much more interest as promising greener and more capable candidates in energy-saving use. In this work, samarium cobalt oxide–decorated reduced graphene oxide (SmCoO3/rGO) was prepared employing solvothermal route and used as reliable electrode material. The maximum specific capacity achieved was 30.80 mAh/g for 1 A/g of SmCoO3/rGO nanocomposite with capacity retention of 86.95%@5A/g over 5000 charge discharge cycles. Better electrochemical performance of samarium and reduced graphene oxide nanostructures prevent the transfer of electrons through electrochemical active sites, creating electronic and structural diversity of electro active material. In addition, SmCoO3/rGO/AC hybrid supercapacitor device that delivered good energy and power density of 52 W h/kg and 752 W/kg at 1 A/g was designed. 74.28% capacitive retention and 98.26% coulombic efficiency was maintained over 15,000 cycles.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Carbon‐nanotube‐reinforced Cu matrix nanocomposites have been fabricated by molecular‐level mixing of functionalized carbon nanotubes (CNTs) with Cu ions, followed by spark plasma sintering. The ...compressive strengths and Young's moduli of CNT‐reinforced nanocomposites are considerably higher than those of the Cu matrix due to the homogeneously dispersed CNTs embedded in the Cu matrix (see Figure).
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BFBNIB, FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SAZU, SBCE, SBMB, UL, UM, UPUK
Abstract
The growth in energy devices and the role of supercapacitors are increasingly important in today’s world. Designing an electrode material for supercapacitors using metals that have high ...performance, superior structure, are eco-friendly, inexpensive and highly abundant is essentially required for commercialization. In this point of view, quaternary chalcogenide Cu
2
NiSnS
4
with fascinating marigold flower like microstructured electrodes are synthesized using different concentrations of citric acid (0, 0.05 M, 0.1 M and 0.2 M) by employing solvothermal method. The electrode materials physicochemical characteristics are deliberated in detail using the basic characterization techniques. The electrochemical studies revealed better electrochemical performances, in particular, Cu
2
NiSnS
4
@0.1 M-CA electrode revealed high 1029 F/g specific capacitance at 0.5 A/g current density. Further, it retained 78.65% capacity over 5000 cycles. To prove the practical applicability, a full-cell asymmetric solid-state device is fabricated, and it delivered 41.25 Wh/Kg and 750 Wh/Kg energy and power density at 0.5 A/g. The optimum citric acid added Cu
2
NiSnS
4
electrode is shown to be a promising candidate for supercapacitor applications.
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IZUM, KILJ, NUK, PILJ, PNG, SAZU, UL, UM, UPUK
Recent evidence shows that dopamine D2-1ike receptor （D2DR） antagonists, such as trifluoperazine and thioridazine, are effective for cancer therapy and inhibition of cancer stem-like cells （CSCs）. In ...this study, we investigated the anti-cancer effects of combination therapy of dexamethasone （DEX） and sulpiride （SUL）, an atypical antipsychotic, against drug-resistant and metastatic breast cancers and further explored the underlying mechanisms. Oral administration of SUL （25, 100 mg.kg-1.d-1） alone did not inhibit the tumor growth in human breast cancer MCF-7/Adr xenograft model vivo. In contrast, combination therapy of SUL （50 mg.kgl-d-1） but dose-dependently decreased the proportion of CSCs in vitro and in and DEX （8 mg.kgl.d 1） markedly suppressed the tumor growth in MCF-7/ Adr xenograft model with little systemic toxicity and lung metastasis in murine metastatic breast cancer 4T1 xenograft model. Among the metastasis-associated biomarkers analyzed, the combination therapy significantly decreased the levels of MMP-2, but increased E-cadherin levels in 4T1 xenograft tumors. Moreover, the combination therapy significantly inhibited the cell colony formation, migration and invasion of 4T1 and human breast cancer MDA-MB-231 cells in vitro. Addition of a specific D2DR agonist 7-OH-DPAT to the combination therapy reversed the enhanced anti-cancer effects in vivo and CSC population loss in tumor tissues. Our data demonstrate that SUL remarkably enhances the efficacy of DEX in the treatment of drug-resistant and metastatic breast cancer via the antagonism of D2DR, which might result from the eradication of CSCs.
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EMUNI, FIS, FZAB, GEOZS, GIS, IJS, IMTLJ, KILJ, KISLJ, MFDPS, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
Botrytis cinerea is a typical necrotrophic pathogenic fungus that causes severe diseases in a wide range of plant species, leading to significant economic losses. Our previous study showed that ...BcSDR1 positively regulates growth, development, and pathogenicity of B. cinerea. However, the regulation mechanism of BcSDR1 and the relationship between BcSDR1 and cAMP and MAPK signaling pathways are not well understood. In this study, transcriptome data showed that BcSDR1 is involved in glucose transmembrane transport, signal transduction, secondary metabolism, and other biological processes. BcSDR1 mutant (BCt41) showed remarkably weak sensitivity to cAMP and MAPK signaling pathways specific inhibitors, SQ22536 and U0126, and significantly decreased cAMP content. The key genes of cAMP and MAPK signaling pathways, BcGB1, BcBTP1, BcBOS1, BcRAS1, and BcBMP3 were significantly upregulated, whereas BcPLC1, BcBCG1, BcCDC4, BcSAK1, BcATF1, and BcBAP1 were significantly downregulated (P<0.05). BcSDR1 was obviously upregulated in BcBCG2, BcBCG3, BcPKA1, and BcPKAR RNA interference (RNAi) mutants, but significantly downregulated in BcPKA2, BcBMP1, and BcBMP3 RNAi mutants. Thus, BcBCG2, BcBCG3, BcPKA1, and BcPKAR negatively regulate BcSDR1 expression, whereas BcPKA2, BcBMP1, and BcBMP3 positively regulate BcSDR1 expression.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
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