The ability to genetically identify and manipulate neural circuits in the mouse is rapidly advancing our understanding of visual processing in the mammalian brain 1, 2. However, studies investigating ...the circuitry that underlies complex ethologically relevant visual behaviors in the mouse have been primarily restricted to fear responses 3–5. Here, we show that a laboratory strain of mouse (Mus musculus, C57BL/6J) robustly pursues, captures, and consumes live insect prey and that vision is necessary for mice to perform the accurate orienting and approach behaviors leading to capture. Specifically, we differentially perturbed visual or auditory input in mice and determined that visual input is required for accurate approach, allowing maintenance of bearing to within 11° of the target on average during pursuit. While mice were able to capture prey without vision, the accuracy of their approaches and capture rate dramatically declined. To better explore the contribution of vision to this behavior, we developed a simple assay that isolated visual cues and simplified analysis of the visually guided approach. Together, our results demonstrate that laboratory mice are capable of exhibiting dynamic and accurate visually guided approach behaviors and provide a means to estimate the visual features that drive behavior within an ethological context.
•The C57BL/6J strain of mouse exhibits robust prey-capture behavior•Vision is essential for accurate long-range approach behavior•Vision and audition can each contribute to capture at short ranges•A visual approach paradigm reveals the natural operating parameters of mouse vision
Mice are an important model system for studying vision, but relatively few ethological visually guided behaviors performed by laboratory mice have been described. Hoy et al. demonstrate that a common laboratory strain of mouse performs prey capture of live crickets and that vision is essential for accurate approaches leading to capture.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Sensory processing is dependent upon behavioral state. In mice, locomotion is accompanied by changes in cortical state and enhanced visual responses. Although recent studies have begun to elucidate ...intrinsic cortical mechanisms underlying this effect, the neural circuits that initially couple locomotion to cortical processing are unknown. The mesencephalic locomotor region (MLR) has been shown to be capable of initiating running and is associated with the ascending reticular activating system. Here, we find that optogenetic stimulation of the MLR in awake, head-fixed mice can induce both locomotion and increases in the gain of cortical responses. MLR stimulation below the threshold for overt movement similarly changed cortical processing, revealing that MLR’s effects on cortex are dissociable from locomotion. Likewise, stimulation of MLR projections to the basal forebrain also enhanced cortical responses, suggesting a pathway linking the MLR to cortex. These studies demonstrate that the MLR regulates cortical state in parallel with locomotion.
•Locomotion induces changes in cortical state and visual processing in mice•Optogenetic stimulation of mesencephalic locomotor region (MLR) initiates locomotion•Subthreshold stimulation of MLR induces cortical changes in the absence of locomotion•The MLR projection to basal forebrain provides a circuit to link locomotion to cortex
Running has previously been shown to induce changes in visual cortical processing in the mouse. In this study, Lee et al. identify the neural circuit in the mesencephalic locomotor region that initially couples locomotion with cortical changes.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
The laminar structure and conserved cellular organization of mouse visual cortex provide a useful model to determine the mechanisms underlying the development of visual system function. However, the ...normal development of many receptive field properties has not yet been thoroughly quantified, particularly with respect to layer identity and in the absence of anesthesia. Here, we use multisite electrophysiological recording in the awake mouse across an extended period of development, starting at eye opening, to measure receptive field properties and behavioral-state modulation of responsiveness. We find selective responses for orientation, direction, and spatial frequency at eye opening, which are similar across cortical layers. After this initial similarity, we observe layer-specific maturation of orientation selectivity, direction selectivity, and linearity over the following week. Developmental increases in selectivity are most robust and similar between layers 2-4, whereas layers 5 and 6 undergo distinct refinement patterns. Finally, we studied layer-specific behavioral-state modulation of cortical activity and observed a striking reorganization in the effects of running on response gain. During week 1 after eye opening, running increases responsiveness in layers 4 and 5, whereas in adulthood, the effects of running are most pronounced in layer 2/3. Together, these data demonstrate that response selectivity is present in all layers of the primary visual cortex (V1) at eye opening in the awake mouse and identify the features of basic V1 function that are further shaped over this early developmental period in a layer-specific manner.
The superior colliculus (SC) plays a highly conserved role in visual processing and mediates visual orienting behaviors across species, including both overt motor orienting 1, 2 and orienting of ...attention 3, 4. To determine the specific circuits within the superficial superior colliculus (sSC) that drive orienting and approach behavior toward appetitive stimuli, we explored the role of three genetically defined cell types in mediating prey capture in mice. Chemogenetic inactivation of two classically defined cell types, the wide-field (WF) and narrow-field (NF) vertical neurons, revealed that they are involved in distinct aspects of prey capture. WF neurons were required for rapid prey detection and distant approach initiation, whereas NF neurons were required for accurate orienting during pursuit as well as approach initiation and continuity. In contrast, prey capture did not require parvalbumin-expressing (PV) neurons that have previously been implicated in fear responses. The visual coding and projection targets of WF and NF cells were consistent with their roles in prey detection versus pursuit, respectively. Thus, our studies link specific neural circuit connectivity and function with stimulus detection and orienting behavior, providing insight into visuomotor and attentional mechanisms mediated by superior colliculus.
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•Cell types in the superior colliculus differentially contribute to prey capture•Wide-field vertical neurons are required for prey detection and approach initiation•Narrow-field vertical neurons are required for accurate and continuous approaches•Connectivity and visual responses are consistent with the role in behavior
The superior colliculus (SC) plays a conserved role in orienting toward stimuli, but the cell-type-specific circuits mediating this are not well understood. Hoy et al. study genetically defined cell types of the SC in visually guided prey capture in the mouse and demonstrate their roles in detecting stimuli versus accurate orienting and approach.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Specific features of visual objects innately draw approach responses in animals, and provide natural signals of potential reward. However, visual sampling behaviours and the detection of salient, ...rewarding stimuli are context and behavioural state-dependent and it remains unclear how visual perception and orienting responses change with specific expectations. To start to address this question, we employed a virtual stimulus orienting paradigm based on prey capture to quantify the conditional expression of visual stimulus-evoked innate approaches in freely moving mice. We found that specific combinations of stimulus features selectively evoked innate approach or freezing responses when stimuli were unexpected. We discovered that prey capture experience, and therefore the expectation of prey in the environment, selectively modified approach frequency, as well as altered those visual features that evoked approach. Thus, we found that mice exhibit robust and selective orienting responses to parameterized visual stimuli that can be robustly and specifically modified via natural experience. This work provides critical insight into how natural appetitive behaviours are driven by both specific features of visual motion and internal states that alter stimulus salience.
A synthesis was carried out to examine Alaska’s boreal forest fire regime. During the 2000s, an average of 767 000 ha·year–1 burned, 50% higher than in any previous decade since the 1940s. Over the ...past 60 years, there was a decrease in the number of lightning-ignited fires, an increase in extreme lightning-ignited fire events, an increase in human-ignited fires, and a decrease in the number of extreme human-ignited fire events. The fraction of area burned from human-ignited fires fell from 26% for the 1950s and 1960s to 5% for the 1990s and 2000s, a result from the change in fire policy that gave the highest suppression priorities to fire events that occurred near human settlements. The amount of area burned during late-season fires increased over the past two decades. Deeper burning of surface organic layers in black spruce (Picea mariana (Mill.) BSP) forests occurred during late-growing-season fires and on more well-drained sites. These trends all point to black spruce forests becoming increasingly vulnerable to the combined changes of key characteristics of Alaska’s fire regime, except on poorly drained sites, which are resistant to deep burning. The implications of these fire regime changes to the vulnerability and resilience of Alaska’s boreal forests and land and fire management are discussed.
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BF, DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
There has been considerable interest in the recent literature regarding the assessment of post-fire effects on forested areas within the North American boreal forest. Assessing the physical and ...ecological effects of fire in boreal forests has far-reaching implications for a variety of ecosystem processes - such as post-fire forest succession - and land management decisions. The present paper reviews past assessments and the studies presented in this special issue that have largely been based on the Composite Burn Index and differenced Normalized Burn Ratio (dNBR). Results from relating and mapping fire/burn severity within the boreal region have been variable, and are likely attributed, in part, to the wide variability in vegetation and terrain conditions that are characteristic of the region. Satellite remote sensing of post-fire effects alone without proper field calibration should be avoided. A sampling approach combining field and image values of burn condition is necessary for successful mapping of fire/burn severity. Satellite-based assessments of fire/burn severity, and in particular dNBR and related indices, need to be used judiciously and assessed for appropriateness based on the users' need. Issues unique to high latitudes also need to be considered when using satellite-derived information in the boreal forest region.
Integration of binocular information at the cellular level has long been studied in the mouse model to uncover the fundamental developmental mechanisms underlying mammalian vision. However, we lack ...an understanding of the corresponding ontogeny of visual behavior in mice that relies on binocular integration. To address this major outstanding question, we quantified the natural visually guided behavior of postnatal day 21 (P21) and adult mice using a live prey capture assay and a computerized-spontaneous perception of objects task (C-SPOT). We found a robust and specific binocular visual field processing deficit in P21 mice as compared to adults that corresponded to a selective increase in c-Fos expression in the anterior superior colliculus (SC) of the juveniles after C-SPOT. These data link a specific binocular perception deficit in developing mice to activity changes in the SC.
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•Juvenile (P21) mice robustly investigate live insects•Insect pursuit behavior relying on binocular vision is immature in P21 mice•Visually induced arrest responses are similar between P21 and adult mice•c-Fos expression selectively increases in juvenile SC after visual experience
Biological sciences, Neuroscience, Sensory neuroscience.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Barker et al. proposed that low birth weight predisposes to higher death rates in adult life. We previously confirmed this fact in a cohort of young adults who were born in a remote Australian ...Aboriginal community between 1956 and 1985. We now present data in these same people with four more years of follow-up and a greater number of deaths. The fates of participants were documented from age 15 years until death, start of dialysis, or until the end of 2010 and causes of death were derived from clinic narratives and dialysis records. Rates of natural deaths were compared by birth cohorts and birth weight, and hazard ratios were calculated using Cox proportional hazards methods, by birth weight and adjusted for birth cohort and sex. Over follow-up of 19,661 person-years, 61 people died of natural causes between age 15 and the censor date. Low birth weights (<2.5 kg) were associated with higher rates of natural death, with HR (95% CI) 1.76 (1.1–2.9, P=0.03), after adjustment for year of birth and sex. The effect was particularly prominent for deaths at <41 years of age, and with deaths from respiratory conditions/sepsis and unusual causes. A predisposing effect of low birth weight on adult deaths was confirmed. This phenomenon, occurring in the context of dramatically improved survivals of lower birth weight infants and children since the early 1960s, helps explain the current epidemic of chronic disease in Aboriginal people. Birth weights continue to improve, so excess deaths from this source should progressively be minimized.
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