A higher CD34+ cell dose is associated with improved engraftment but may also be associated with an increased risk of complications after allogeneic hematopoietic stem cell transplantation, including ...graft-versus-host disease (GVHD).
We retrospectively analyze the impact of CD34+ cell dose on OS, PFS, neutrophil engraftment, platelet engraftment, treatment-related mortality, and GVHD grading.
For analyses, CD34+ cell dose was stratified into low (< 8.5 × 106/kg) and high (> 8.5 × 106/kg). A subgroup analysis of higher CD34+ cell dose leads to prolonged OS and PFS, but statistical significance was achieved only for PFS (OR 0.36; 95%CI 0.14-0.95; P = 0.04).
This study reinforced that CD34+ cell dose at the time of allo-HSCT retained a positive impact on PFS.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
John Lyly's Galatea (1584) is a court comedy of virtue, which was written specifically to be performed before Elizabeth I. Composed after Campaspe (1583) and Sapho and Phao (1584), Galatea is the ...third in Lyly's first series of court comedies, and situated within the author's successive approaches to the cult of Elizabeth I, who was worshipped as the Maiden Queen of England and the Virgin Mother of her nation. Furthermore, after the playwright's first prose fiction work, Euphues: The Anatomy of Wit (1578), Galatea is also Lyly's first play to revise the motif of father-daughter disagreement and to rethink conflicting male likenesses. Through double cross-dressing, two females' love of being alike is thus dramatized in response to the dysfunction of patriarchal authority. Accordingly, this article explores the way in which certain figures or ideas are reproduced, even mutated through imitation, as memes in the play relating to the queen's virtue. Given the presence of the queen at the play's performance, this article explores how far Lyly redefines the subordinate relationship of the on-stage subjects to the off-stage Queen Elizabeth I: examining how the sacrificial virgins, vagrant boys, and cross-dressed maidens of Galatea seek various routes to the same shelter under the eye of the constant and multidimensional queen who stands in as an exemplar of divine perfection.
We present a sprayed composite thin film, comprising Cu−In‐Zn−S (CIZS) particles embedded in ZnO matrix for photoelectrochemical hydrogen production from water splitting. CIZS, a photoactive ...semiconductor was used as a photon absorber, whereas ZnO channels as the pathway for charge transfer. It was found that the distribution of the CIZS particles had a direct impact on the photoelectrochemical (PEC) activity. A more homogeneous dispersion of smaller CIZS particles (0.56 μm) within ZnO matrix exhibited a higher photocurrent density, and 3.27 μmol/ cm2 hydrogen evolution for 5 h. Electrochemical impedance spectroscopy (EIS) was employed to analyze the charge transfer mechanism of this composite thin film. In addition, ZnO coating on top of CIZS particles also served as the adhesion and protection layers. All the PEC experiments were performed in 0.5 M K2SO4 electrolyte. No sacrificial reagents were used. The composite electrode was stable under illumination: 75.67 % of photo‐activity remained after 1 h illumination at a bias of 0.2 V vs. SCE. This study demonstrates a simple and low‐cost spray preparation of composite thin film consisting of particles embedded in any semiconductor matrix.
In the PEC experiments, no sacrificial reagents were used. The sample C3 with more homogeneous dispersion of smaller CIZS particles within ZnO matrix exhibited a higher photocurrent. 3.27 μmol/cm2 of produced hydrogen was collected in 5 hours. This work provided a simple and low‐cost way to prepare a heterostructured composite photoelectrode.
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FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SAZU, SBCE, SBMB, UL, UM, UPUK
Overcoming the diffraction limit is a great challenge to increasing the nanowell density. A methodology differing from super-resolution microscopy is introduced to realize a theoretical √2- or ...√3-fold resolution improvement via two-step imaging and simple equipment adaptations: (1) a biochip integrated with an orthogonal-orientation nano/micropolarizer array in an interspaced arrangement with reaction sites and (2) an optical system inserted with an angle-switchable polarizer plate to generate polarized light for illumination. Because the 0° polarized light will be blocked by the 90° polarizer units, only half of the signals on the polarizer array with the same polarization angle can be acquired, and a virtual distance occurs between any two “bright” fluorescence signals. We have demonstrated that a 0.9 μm pitch wide-field image unresolvable via a 20×/NA0.5 objective can be divided into two resolvable images with an 8–10 signal-to-noise ratio (SNR) based on this approach. The aluminum polarizer array on the biochip had a 180 nm thickness, 0.9 μm pixel size, 300 nm period, and ∼0.55 fill factor. This method could be used for practical fluorescence-based nanoarray analysis, such as next-generation sequencing (NGS), to either enlarge the field of view to accelerate the scanning speed of a whole chip or increase the reaction density for multiple throughputs, and thus the sequencing cost can be reduced.
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IJS, KILJ, NUK, PNG, UL, UM
Iron is an essential micronutrient for the brain development of the fetus. Altered intestinal microbiota might affect behavior and cognition through the so-called microbiota-gut-brain axis. We used a ...Sprague-Dawley rat model of a maternal low-iron diet to explore the changes in cognition, dorsal hippocampal brain-derived neurotrophic factor (BDNF) and related pathways, gut microbiota, and related metabolites in adult male offspring. We established maternal iron-deficient rats by feeding them a low-iron diet (2.9 mg/kg), while the control rats were fed a standard diet (52.3 mg/kg). We used a Morris water maze test to assess spatial learning and long-term memory. Western blot (WB) assays and a quantitative reverse-transcription polymerase chain reaction (qRT-PCR) were used to detect the BDNF concentration and related signaling pathways. We collected fecal samples for microbiota profiling and measured the concentrations of plasma short-chain fatty acids. The adult male offspring of maternal rats fed low-iron diets before pregnancy, during pregnancy and throughout the lactation period had (1) spatial deficits, (2) a decreased BDNF mRNA expression and protein concentrations, accompanied by a decreased TrkB protein abundance, (3) a decreased plasma acetate concentration, and (4) an enrichment of the
genus
and
genus
. Maternal iron deficiency leads to an offspring spatial deficit and is associated with alternations in gastrointestinal microbiota and metabolites.
Idiopathic membranous nephropathy (MN) is an autoimmune-mediated glomerulonephritis and the most common cause of idiopathic nephrotic syndrome in adult humans. A tumor necrosis factor α ...(TNF-α)-mediated inflammatory response via TNF receptor 1 (TNFR1) and TNFR2 has been proposed as a pathogenic factor. In this study, we assessed the therapeutic response to blocking TNF signaling in experimental MN. Murine MN was induced experimentally by cationic bovine serum albumin (cBSA); phosphate-buffered saline was used in control mice. In MN mice, TNF was inhibited by etanercept blocking of TNFR1/TNFR2 or the preligand assembly domain fusion protein (PLAD.Fc), a small fusion protein that can preferentially block TNFR1 signaling. Disease severity and possible mechanisms were assessed by analyzing the metabolic and histopathology profiles, lymphocyte subsets, immunoglobulin production, oxidative stress, and apoptosis. cBSA-induced MN mice exhibited typical nephrotic syndrome and renal histopathology. MN mice given etanercept or PLAD.Fc did not exhibit significant reduction of proteinuria, amelioration of glomerular lesions, or attenuation of immune complex deposition. Immune cell subsets, serum immunoglobulin levels, production of reactive oxygen species, and cell apoptosis in the kidney were not altered by TNF inhibition. By contrast, MN mice receiving etanercept or PLAD.Fc exhibited significantly decreased infiltration of immune cells into the kidney. These results show that the therapeutic effects of blocking TNFR1 and/or TNFR2 signaling in experimental MN are not clinically effective. However, TNF signaling inhibition significantly attenuated renal immune cell infiltration in experimental MN.
Plants have evolved diverse cellular mechanisms to maintain their fitness under stressful environments. Banana (Musa spp.) plants inoculated with a new rhizobacterial isolate, Pseudomonas aeruginosa ...strain Y1 (PaY1), showed improved vegetative growth, and increased levels of H2O2, glucosinolates, phenolic compounds, and lignin contents. Plants treated with PaY1 had reduced membrane peroxidation and electrolyte leakages, and exhibited higher PSII efficiencies after drought and submergence treatments. Furthermore, PaY1 treatment reduced disease infection rates in the Foc TR4 (Fusarium wilt pathogen)-inoculated plants. RNA seq results identified signaling molecules activated by PaY1, including ROS/antioxidant molecules and signaling of G protein and sphingolipids. Besides, PaY1 exerted positive effects on hormonal signaling, including auxin, ABA, and jasmonic acid (JA) in banana seedlings. Genes associated with cellular pathways of cell division and flowering control were significantly affected; up-regulated genes were also implicated in cellular pathways regulating nutrient availability. The up-regulated genes functioning in abiotic stress tolerance comprised genes encoding DETOXIFICATION-like proteins, aquaporins, autophagy-like, and MAPKK2-like. Furthermore, genes encoding proteins participating in the regulatory network of disease resistance were discovered, which included members of pathogenesis-related proteins (PRs), cell death associated proteins, proteins implicated in the MAMP-trigger immunity (MTI), and genes such as RGA2-like and ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR 1B (ERF1B) with predicted functions regulating disease resistance against Fusarium oxysporum in different plant species. The transcriptome data presented here indicated that PaY1 is a potent activator of banana genes related to hormonal signaling and cellular pathways including antioxidant defense, detoxification, and MTI, which worked collaboratively to control plant growth, abiotic and biotic stress tolerance in banana plants.
•A growth-promoting bacterial strain, PaY1, enhanced tolerance of drought and hypoxia, and increased resistance of Fusarium wilt in banana plants.•The activated signaling molecules including ROS/antioxidant molecules, G protein, and sphingolipids were identified in the PaY1 treated plants.•Phytohormone signaling including auxin, ABA, and JA signaling were poistively regulated in the treated plants.•Banana genes associated with cellular pathways of cell division and flowering control were significantly affected by PaY1 treatment.•PaY1 activated expression of genes involved in both abiotic stress tolerance and disease resistance against Fusarium wilt.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Summary The incidence of pulmonary nodules after cardiac transplantation is not uncommon, and prompt diagnostic procedures are necessary to minimize disease-related morbidity and mortality. We report ...a 56-year-old woman who was found to have bilateral pulmonary nodules four months after cardiac transplantation. The microorganism was identified with a molecular diagnostic method as Schizophyllum commune , which had not been reported in English literature as a pathogen inducing pulmonary nodules after transplantation. She remained asymptomatic during the therapeutic period and the pulmonary nodules resolved six months later.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK
Background: Patients often exhibit preoperative fear and anxiety that may influence the process of induction and recovery from anesthesia. Music is thought to be an alternative to medication for ...relief of fear and anxiety. Objectives: The purpose of the present study was to explore the feasibility of using heart rate (HR) variability (HRV) for evaluating the efficacy of music listening to relieve the patients’ anxiety during their stay in the operation room waiting area and to compare the HRV measures with subjective Visual Analogue Scale (VAS) scores. Methods: In total, 140 patients were randomly assigned to the experimental (n = 64) or control group (n = 76). The intervention consisted of a 10-min period of exposure to relaxing music delivered through headphones. Anxiety levels were measured by VAS (a 10-point scale) and 5 min of HRV monitoring before and after the music intervention. Results: The music group demonstrated significant reductions in VAS scores, mean HR, low-frequency HRV, and low- to high-frequency ratio and an increase in high-frequency HRV, while patients in the control group showed no changes. The subjective results of patients’ VAS anxiety scores were consistent with the objective results of HRV parameters. Conclusions: Listening to music can significantly lower the anxiety levels of patients before surgery. The frequency-domain parameters of HRV can be indicators for monitoring the change in anxiety level of preoperative patients.
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NUK, OILJ, SAZU, UKNU, UL, UM, UPUK, VSZLJ
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