Histone deacetylase (HDAC) inhibitors augment ionizing radiation (IR)-induced apoptosis in several cancer cells by undefined mechanism(s). We recently found that the HDAC inhibitors induce a BH3-only ...protein Bmf in human squamous carcinoma SAS cells. We extended this study and found that 2.5 nM FK228 pretreatment could not induce apoptosis but augmented IR-induced death. The FK228 pretreatment increased Bmf expression level, and siRNA-mediated knockdown of Bmf transcripts strongly inhibited its augmentation of IR-induced cell death, disruption of mitochondrial membrane potential and DNA fragmentation. Another HDAC inhibitor CBHA pretreatment similarly augmented IR-induced apoptosis, and this effect was also inhibited by Bmf knockdown. Bmf overexpression augmented IR-induced death, and the augmented effects of FK228 were similarly observed in another squamous carcinoma HSC2 cells. Overexpression of histone acetyltransferase p300 mimicked the effects of the HDAC inhibitors, i.e., it enhanced IR-induced death, which was mostly abolished by Bmf knockdown. Taken together, histone hyperacetylation may enhance IR-induced death via activation of Bmf transcription, thereby implying Bmf as a key molecule for HDAC inhibitors (FK228 and CBHA)-mediated enhancing effect on IR-induced cell death.
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EMUNI, FIS, FZAB, GEOZS, GIS, IJS, IMTLJ, KILJ, KISLJ, MFDPS, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
In 2012, the Norwegian newborn screening program (NBS) was expanded (eNBS) from screening for two diseases to that for 23 diseases (20 inborn errors of metabolism, IEMs) and again in 2018, to include ...a total of 25 conditions (21 IEMs). Between 1 March 2012 and 29 February 2020, 461,369 newborns were screened for 20 IEMs in addition to phenylketonuria (PKU). Excluding PKU, there were 75 true-positive (TP) (1:6151) and 107 (1:4311) false-positive IEM cases. Twenty-one percent of the TP cases were symptomatic at the time of the NBS results, but in two-thirds, the screening result directed the exact diagnosis. Eighty-two percent of the TP cases had good health outcomes, evaluated in 2020. The yearly positive predictive value was increased from 26% to 54% by the use of the Region 4 Stork post-analytical interpretive tool (R4S)/Collaborative Laboratory Integrated Reports 2.0 (CLIR), second-tier biochemical testing and genetic confirmation using DNA extracted from the original dried blood spots. The incidence of IEMs increased by 46% after eNBS was introduced, predominantly due to the finding of attenuated phenotypes. The next step is defining which newborns would truly benefit from screening at the milder end of the disease spectrum. This will require coordinated international collaboration, including proper case definitions and outcome studies.
Unlike traditional opportunistic networks characterized by relatively very short connectivity period among mobile devices during their contacts, in this paper, we explicitly consider the ...underground/bus scenario where the inherited structured mobility pattern enforces more stable passenger behaviors. If with the aid of potential formation of Bluetooth-enabled multi-hop wireless networks, one can expect a type of new emerging applications for passengers on the move. In particular, we conducted a thorough experimental study on passenger dynamics and connectivities in Beijing Underground during peak hours, via building an information collection application in an Android-based smartphone. From the total of 41,806 records for 120 days, we have performed extensive analysis on a variety of statistics for the number of neighbors, the lifetime of the neighbors, the received signal strength indicator (RSSI) and its derived physical distance, flow speed of the nearby passengers, battery usage rate, etc. Then, we further prove our justification that Bluetooth is the perfect technology to build up a relatively small multi-hop wireless network with the network size of four nodes, and in particular it is applicable and may fundamentally drive new applications in an underground environment during the peak hour, where two notable examples are music album sharing and neighborhood gaming.
Background
The radix of
Angelica sinensis
is widely used as a medicinal herbal and metabolomics research of this plant during growth is necessary.
Results
Principal component analysis of the ...UPLC-QTOFMS data showed that these 27 samples could be separated into 4 different groups. The chemical markers accounting for these separations were identified from the PCA loadings plot. These markers were further verified by accurate mass tandem mass and retention times of available reference standards. The study has shown that accumulation of secondary metabolites of
Angelica sinensis
is closely related to the growth periods.
Conclusions
The UPLC-QTOFMS based metabolomics approach has great potential for analysis of the alterations of secondary metabolites of
Angelica sinensis
during growth.
Glycaemic index and glycaemic load of selected popular foods consumed in Southeast Asia Sun, Lijuan; Davina Elizabeth Mei LeeauthorClinical Nutrition Research Centre, Singapore Institute for Clinical Sciences, ASTAR, Singapore; Wei Jie Kevin TanauthorClinical Nutrition Research Centre, Singapore Institute for Clinical Sciences, ASTAR, Singapore ...
2015
Journal Article
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