The Botryosphaeriaceae represents an important, cosmopolitan family of pathogens infecting woody plants. Grapevines (Vitis vinifera) suspected of being affected by Botryosphaeria dieback were ...collected from different Provinces of China and several Botryosphaeriaceae species were identified. This research was conducted to further study the species of Botryosphaeriaceae associated with grapevines in China and to estimate the prevalence and severity of the disease. Symptoms were characterized by partial or total death of grapevine cordons, with brown U-shaped necrotic sectors and brownish-black spots in cross-sections of affected trunks and arms. A fungus isolated from diseased vines in Henan and Anhui Provinces was identified as Neofusicoccum mangiferae on the basis of its morphological and cultural characteristics as well as an analysis of combined sequence data from the internal transcribed spacer (ITS) cluster of the ribosomal DNA together with partial sequences of the β-tubulin and translation elongation factor (EF1-α) genes. Pathogenicity was assessed by inoculating the fungus onto detached green grapevine shoots under controlled laboratory conditions and mature wood of standing vines cv. Summer Black in a greenhouse. This is the first record of N. mangiferae associated with grapevine dieback in the world.
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BFBNIB, IZUM, KILJ, NMLJ, NUK, PILJ, PNG, SAZU, UL, UM, UPUK
A Dothideomycete species, associated with leaf spots of sugarcane (Saccharum officinarum), was collected from Nakhonratchasima Province, Thailand. A single ascospore isolate was obtained and formed ...the asexual morph in culture. ITS, LSU, RPB2 and TEF1α gene regions were sequenced and analyzed with molecular data from related taxa. In a phylogenetic analysis the new isolate clustered with Leptosphaerulina americana, L. arachidicola, L. australis and L. trifolii (Didymellaceae) and the morphology was also comparable with Leptosphaerulina species. Leptosphaerulina saccharicola is introduced to accommodate this new collection which is morphologically and phylogenetically distinct from other species of Leptosphaerulina. A detailed description and illustration is provided for the new species, which is compared with similar taxa. The type specimen of Pleosphaerulina oryzae, is transferred to Leptosphaerulina. It is redescribed and is a distinct species from L. australis, with which it was formerly synonymized. Leptosphaerulina species have been linked to Pithomyces but the lack of phylogenetic support for this link is discussed. The character of the asexual morph of Leptosphaerulina, which is similar to Pithomyces, may to have evolved on separate occasions.
There have been few studies on the taxonomy and biodiversity of the genus Lentinus in Thailand, which is a genus of edible mushrooms. Recently, collections from 17 sites in northern Thailand yielded ...47 specimens of Lentinus sensu lato. Three were shown to be new species of Lentinus sensu stricto and Lentinus roseus, L. concentricus and L. megacystidiatus are introduced in this paper. The new species are described and illustrated with line drawings and are justified and compared with similar taxa. Furthermore, ITS sequence data do not match closely with any species presently lodged in GenBank.
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EMUNI, FIS, FZAB, GEOZS, GIS, IJS, IMTLJ, KILJ, KISLJ, MFDPS, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
A new species of
,
(Togniniaceae), was isolated during a diversity study of freshwater fungi from Yunnan Province in China. Morphological and cultural studies of the fungus were carried out and its ...sexual and asexual morphs (holomorph) are introduced herein. This species is characterised by peculiar long-necked, semi-immersed ascomata with oval to ellipsoid ascospores and ellipsoid to ovoid conidia. Phylogenetic analyses of a combined TUB and ACT gene dataset revealed that strains of
constitute a strongly supported independent lineage and are related to
and
. The number of nucleotide differences, across the genes analysed, also supports establishment of
as a new species.
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IZUM, KILJ, NUK, PILJ, PNG, SAZU, UL, UM, UPUK
— A new taxon of Botryosphaeriaceae associated with Arrhenatherum and Dactylis was collected in Italy. Single ascospore isolates were obtained and formed asexual morphs in culture. Multi-gene ...phylogenetic analysis was carried out using ITS, LSU nrDNA and EF1-α sequence data and the resulting phylogenetic tree showed that new species clustered in Botryosphaeriaceae together with Tiarosporella species with high bootstrap support. The new species is introduced as a sexual morph of Tiarosporella and detailed descriptions and illustrations are provided. A discussion of the asexual morphs of Tiarosporella is provided.
Two new endophytic species,
and.
were isolated from
in Guizhou and Hunan provinces, China. Detailed descriptions and illustrations of these new taxa are provided and morphological comparisons with ...similar taxa are explored. Phylogenetic analysis with combined sequence data (ITS, GAPDH, ACT and TUB2) demonstrated that both species formed distinct clades in this genus. This is the first record of
species from
in China.
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Two novel species, Pestalotiopsis shorea from Shorea obtusa and P. simitheae from Pandanus sp. were isolated in Thailand. They are introduced in this paper on the basis of morphological and molecular ...characteristics. P. shorea differs in morphology from its closely related species, P. adusta, by its shorter, tubular apical appendages and brown median cells; P. simitheae can be separated from its sister species, P. theae, by its shorter apical appendages, larger length/width ratio and colony with irregular edge and being orange on reverse of PDA plates. Phylogenetic analysis based on a combined sequence dataset from the internal transcribed spacer (ITS), partial β-tubulin and partial translation elongation factor 1-alpha (tef??1) gene loci, confirms their distinct phylogenetic positions in the genus with strong support.
Twenty-six strains representing three species of Colletotrichum were isolated from leaf and fruit lesions of vitaceous plants in Guizhou and Yunnan provinces, China. The strains were characterized by ...morphology and phylogenetic analyses of actin, β-tubulin, calmodulin, glutamine synthetase, glyceraldehyde-3-phosphate dehydrogenase and rDNA internal transcribed spacer gene sequences. The combined dataset showed that 20 of 26 strains represented a novel species, the rest being Colletotrichumfructicola (four strains) and Colletotrichumgloeosporioides (two strains). The new species is described herein as Colletotrichumviniferum. Its conidia, compared with similar Colletotrichum species are cylindrical and 12–16 μm long. Based on pathogenicity tests, C. viniferum caused leaf spots and anthracnose of table grape but was not host-specific.
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