A novel mutant of Arabidopsis (Arabidopsis thaliana), having highly glossy inflorescence stems, postgenital fusion in floral organs, and reduced fertility, was isolated from an ethyl ...methanesulfonate-mutagenized population and designated glossyheadl (gsd1). The gsd1 locus was mapped to chromosome 1, and the causal gene was identified as a new alíele of Acetyl-Coenzyme A Carboxylasel (ACC1), a gene encoding the main enzyme in cytosolic malonyl-coenzyme A synthesis. This, to our knowledge, is the first mutant alíele of ACC1 that does not cause lethality at the seed or early germination stage, allowing for the first time a detailed analysis of ACC1 function in mature tissues. Broad lipid profiling of mature gsd1 organs revealed a primary role for ACC1 in the biosynthesis of the very-long-chain fatty acids (C20:0 or longer) associated with cuticular waxes and triacylglycerols. Unexpectedly, transcriptome analysis revealed that gsd1 has limited impact on any lipid metabolic networks but instead has a large effect on environmental stress-responsive pathways, especially senescence and ethylene synthesis determinants, indicating a possible role for the cytosolic malonyl-coenzyme A-derived lipids in stress response signaling.
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Abstract
Background
During 2017, a multistate outbreak investigation occurred after the confirmation of Seoul virus (SEOV) infections in people and pet rats. A total of 147 humans and 897 rats were ...tested.
Methods
In addition to immunoglobulin (Ig)G and IgM serology and traditional reverse-transcription polymerase chain reaction (RT-PCR), novel quantitative RT-PCR primers/probe were developed, and whole genome sequencing was performed.
Results
Seventeen people had SEOV IgM, indicating recent infection; 7 reported symptoms and 3 were hospitalized. All patients recovered. Thirty-one facilities in 11 US states had SEOV infection, and among those with ≥10 rats tested, rat IgG prevalence ranged 2%–70% and SEOV RT-PCR positivity ranged 0%–70%. Human laboratory-confirmed cases were significantly associated with rat IgG positivity and RT-PCR positivity (P = .03 and P = .006, respectively). Genomic sequencing identified >99.5% homology between SEOV sequences in this outbreak, and these were >99% identical to SEOV associated with previous pet rat infections in England, the Netherlands, and France. Frequent trade of rats between home-based ratteries contributed to transmission of SEOV between facilities.
Conclusions
Pet rat owners, breeders, and the healthcare and public health community should be aware and take steps to prevent SEOV transmission in pet rats and to humans. Biosecurity measures and diagnostic testing can prevent further infections.
An investigation of Seoul virus infections in pet rats and people in the US found 31 infected facilities and 17 people with recent infections—all recovered with 3 hospitalized. Frequent trade of rats contributed to transmission of SEOV between facilities.
We report nuclear reaction data for neutron induced reactions on unstable nuclei are critical for a wide range of applications spanning studies of nuclear astrophysics, nuclear reactor designs, and ...radiochemistry diagnostics. However, nuclear data evaluations of the reaction cross sections are largely based on calculations due to the difficulty in performing this class of measurements and the resulting lack of experimental data. For neutron induced charged particle reactions at fast neutron energies, at the MeV scale, these cross section predictions are predominately driven by statistical Hauser-Feshbach calculations. In this work, we present partial and total 59Ni(n, p) and 59Ni(n, α) cross sections, measured directly with a radioactive 59Ni target, and compare the results to the present nuclear data evaluations. In addition, the results from this work are compared to a recent study of the 59Ni(n, xp) reaction cross section that was performed via an indirect surrogate ratio method. The expected energy trend of the cross section, based on the current work, is inconsistent with that of the surrogate work. This calls into question the reliability of that application of the surrogate ratio method and highlights the need for direct measurements on unstable nuclei, when feasible.
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34.
Front Matter R. Gregory Jenks
Paul and His Mortality,
12/2015
Book Chapter
Introduction R. Gregory Jenks
Paul and His Mortality,
12/2015
Book Chapter
No one knows for certain when, where, or how the Apostle Paul died. Tradition sets the year of his death either in A.D. 64 or in late A.D. 67 / early A.D. 68 and puts the place of his death in Rome. ...Tradition also says that Paul was a martyr and that he was beheaded at the bequest of Caesar Nero. Some say he was martyred after having been imprisoned in Rome with the Apostle Peter, who himself was martyred shortly thereafter. The annals of Christianity have different embedded traditions regarding Paul’s death.²
The relative obscurity of Paul’s death is
Paul’s death rests in the shadow of the light of Christ’s death and resurrection. Paul sees the answers to humanity’s ultimate questions regarding life and death not in an analysis of his own life ...and death and contemplating his mortality, but by focusing on Christ’s death and resurrection, the transcendent act and example of Jesus. He views and values his own death to the degree that it points to, exposes, and glorifies Christ’s death. So when Paul talks about his death or his mortality fundamental questions of his relationship with Jesus’ death are raised.¹ How could he best give his
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