Many studies have shown that the mycoplasmal membrane protein p37 enhances cancer cell migration, invasion, and metastasis. Previously, we generated 6 monoclonal antibodies (MAbs) against the ...mycoplasmal protein p37 and showed the presence of mycoplasma-infected circulating tumor cells in the blood of hepatocellular carcinoma patients by using CA27, one of the six MAbs. When mycoplasmas were incubated with cancer cells in the presence of CA27, mycoplasma infection was completely inhibited, suggesting that CA27 is a neutralizing antibody inhibiting mycoplasma infection. To examine the neutralizing epitope of CA27, we generated a series of glutathione S-transferase (GST)-fused p37 deletion mutant proteins in which p37 was partly deleted. To express p37-coding sequences in E.coli, mycoplasmal TGA codons were substituted with TGG in the p37 deletion mutant genes. GST-fused p37 deletion mutant proteins were then screened to identify the epitope targeted by CA27. Western blots showed that CA27 bound to the residues 216-246 on the middle part of the p37 protein while it did not bind to the residues 183-219 and 216-240. Fine mapping showed that CA27 was able to bind to the residues 226-246, but its binding activity was relatively weakened as compared to that to the residues 216-246, suggesting that the residues 226-246 is essential for optimal binding activity of CA27. Interestingly, the treatment of the purified GST-tagged epitopes with urea showed that CA27 binding to the epitope was sodium dodecyl sulfate-resistant but urea-sensitive. The same 226-246 residues were also recognized by two other anti-p37 MAbs, suggesting that the epitope is immunodominant. The identification of the novel neutralizing epitope may provide new insight into the interaction between the p37 protein and host receptors.
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DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
We examined the anticancer effects of a novel sirtuin inhibitor, MHY2256, on HCT116 human colorectal cancer cells to investigate its underlying molecular mechanisms. MHY2256 significantly suppressed ...the activity of sirtuin 1 and expression levels of sirtuin 1/2 and stimulated acetylation of forkhead box O1, which is a target protein of sirtuin 1. Treatment with MHY2256 inhibited the growth of the HCT116 (TP53 wild-type), HT-29 (TP53 mutant), and DLD-1 (TP53 mutant) human colorectal cancer cell lines. In addition, MHY2256 induced G0/G1 phase arrest of the cell cycle progression, which was accompanied by the reduction of cyclin D1 and cyclin E and the decrease of cyclin-dependent kinase 2, cyclin-dependent kinase 4, cyclin-dependent kinase 6, phosphorylated retinoblastoma protein, and E2F transcription factor 1. Apoptosis induction was shown by DNA fragmentation and increase in late apoptosis, which were detected using flow cytometric analysis. MHY2256 downregulated expression levels of procaspase-8, -9, and -3 and led to subsequent poly(ADP-ribose) polymerase cleavage. MHY2256-induced apoptosis was involved in the activation of caspase-8, -9, and -3 and was prevented by pretreatment with Z-VAD-FMK, a pan-caspase inhibitor. Furthermore, the autophagic effects of MHY2256 were observed as cytoplasmic vacuolation, green fluorescent protein-light-chain 3 punctate dots, accumulation of acidic vesicular organelles, and upregulated expression level of light-chain 3-II. Taken together, these results suggest that MHY2256 could be a potential novel sirtuin inhibitor for the chemoprevention or treatment of colorectal cancer or both.
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•A stretchable CdSe/ZnS QD-LEDs array is demonstrated for a visual display of bio-signals.•The island-bridge design with liquid metal interconnections relieves the applied strain.•A ...honeycomb structured Au grid/EG doped PEDOT:PSS is used as a transparent electrode.•The display shows a mechanical stability over 10,000 cycles of 40% stretching deformations.•Knee-bending and skin temperature can be displayed with integrated QD-LEDs patterns.
Display devices that can visually present various bio-signals are a core future technology required for skin attachable wearable electronics. Quantum dot light emitting diodes (QD-LEDs), which can be used as high performance display devices, exhibit many advantages such as a narrow bandwidth, high color purity, high environmental stability, easy and fast processing, and a high brightness at low voltage. In this study, we report on the fabrication of a stretchable QD-LED array for the visual display of body movement and skin temperature signals from skin-attached sensors. After forming an array of a rigid NOA63 islands, an array of CdSe/ZnS QD-LEDs is fabricated on top of that island array and electrically connected using liquid metal Galinstan interconnections. Finally, the array of QD-LEDs on the NOA63 islands is transferred onto a stretchable elastomer substrate of Ecoflex/PDMS. Of particular importance, for stable performance of the QD-LEDs under deformation, in replacement of the conventionally used ITO, a flexible and highly transparent electrode of Au grid/Ethylene glycol doped PEDOT:PSS is used. Such a design architecture can minimize the strain applied to the QD-LEDs by concentrating the strain onto the soft elastomer film substrate and the Galinstan interconnections. As a result, the fabricated QD-LED array exhibits stable operation under both 50% uniaxial and 30% biaxial strains. After attachment of the stretchable QD-LED array onto skin, the extent of knee bending, and changes in skin temperature are displayed as changes in the pattern of the QD-LED array. This work demonstrates the potential application of our stretchable array of QD-LEDs for easy and daily monitoring of health conditions through a visual display.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Here, we report on a highly conductive, stretchable, and transparent electrode of poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS) fabricated via modification with triblock ...copolymer, poly(ethylene glycol)–block–poly(propylene glycol)–block–poly(ethylene glycol) (PEO20–PPO70–PEO20, Pluronic P123), and post-treatment with sulfuric acid. The fabricated electrode exhibits high transparency (89%), high electrical conductivity (∼1700 S/cm), and minimal change in resistance (∼4%) under repetitive stretch–release cycles at 40% tensile strain after stabilization. P123 acts as a secondary dopant and plasticizer, resulting in enhanced electrical conductivity and stretchability of PEDOT:PSS. Furthermore, after sulfuric acid post-treatment, P123 helps the electrode to maintain its stretchability. A successful demonstration of the stretchable interconnection was shown by stretching the P123-modified PEDOT:PSS electrodes, which were connected with light-emitting diodes (LEDs) in series. Finally, a stretchable and transparent touch sensor consisting of our fabricated electrodes and an LED array and stretchable semitransparent supercapacitor were presented, suggesting a great potential of our electrodes in the application to various deformable devices.
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IJS, KILJ, NUK, PNG, UL, UM
We report on the fabrication of a high performance flexible micro-supercapacitor (MSC) for powering a vertically integrated skin-attachable strain sensor on a gecko-inspired micro-structured ...adhesive. Combined utilization of the mixed manganese/vanadium (Mn/V) oxide grown on MWCNT electrode and the sulfone-based electrolyte, PC/SL/LiClO4/PMMA, enhances both the capacitance and operation voltage up to 2 V of MSC. Thus, the fabricated MSC exhibits excellent electrochemical performance with an areal capacitance of 11.8 mF cm−2 and an areal energy density of 6.58 µWh cm−2 at an areal power density of 200 µW cm−2. The MSC shows mechanical stability over 1000 repetitive bends at a bending radius of 3.7 mm. The strain sensor made of fragmentized graphene foam embedded in PDMS film provides a high gauge factor of 12.6 up to 50% strain to detect strains due to various bio-signals. Our gecko-inspired adhesive made of PDMS micropillars inked with a mixture of PDMS and Silbione to have spatula tips exhibits not only high adhesion property but also high durability over repeated cycles of attachment-detachment and negligible skin irritation. After vertical integration of the MSC, strain sensor, and the adhesive film, bio-signals such as an arterial pulse, swallowing, and frowning of the brow are successfully detected using energy stored in the MSC.
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•High-performance micro-supercapacitor (MSC) was fabricated with mixed metal oxide electrode and sulfone-based electrolyte.•Mixed oxide electrode enhanced the capacitance and sulfone-based electrolyte widened operation voltage window of MSC.•Skin-attached fragmentized graphene foam strain sensor with a micro-structured adhesive detected bio-signals.•The strain sensor was operated using the stored energy of the vertically integrated MSC.•MSC was demonstrated as an integrated energy storage device for bio-signal monitoring.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
This paper introduces a liquid-metal integrated system that combines soft electronics materials and engineering designs with advanced near-field-communication (NFC) functionality for human motion ...sensing. All of the active components, that is, strain sensor, antenna and interconnections, in this device are made of liquid metal, and the device has unique gel-like characteristics and stretchability. Patterning procedures based on selective wetting properties of the reduced GaInSn enable a skin-attachable, miniaturized layout, in which the diameter of the device is less than 2 cm. Electromechanical characterization of the strain sensor and antenna reveals their behaviors under large uniaxial tensile and compressive strains, as well as more complex modes of deformation. Demonstrations of these devices involve their use in monitoring various human motions in a purely wireless fashion; examples include wrist flexion, movements of the vocal cord and finger motion. This simple platform has potential for use in human-machine interfaces for prosthetic control and other applications.
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•● We report a fractal-designed transparent and stretchable microsupercapacitor (MSC).•● The fabricated MSC exhibits transparency of 79% with a capacitance of 12.6 mF cm−2.•● The MSC ...maintains electrochemical performance after 2,000 stretching cycles by 30%.•● The strain sensor (SS) can be driven by the integrated MSC.•● The SS powered by the MSC detects wrist bending and arterial pulses.
We report on the fabrication of a fractal-designed transparent and stretchable (TS) microsupercapacitor (MSC) as a skin-attachable energy storage device for driving an integrated strain sensor (SS). The MSC is designed to have a fractal structure with long and narrow patterns to ensure high transparency and stretchability. MnO2/Carbon nanotubes (CNTs) and 1-butyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide/poly(methyl methacrylate) (BMIMTFSI/PMMA) are used as electrodes and a stretchable electrolyte for the MSC, respectively. The fabricated MSC exhibits high transparency of 79% and an areal capacitance of 12.6 mF cm−2 at a current density of 5 mA cm−2. The TS MSC shows excellent mechanical stability in that it can maintain stable electrochemical performance even after 2000 repeated stretching cycles up to 30%. The TS SS is facilely prepared via spray coating of Ag nanowires (NWs) and subsequent curing of PDMS, where the transparency and the electrical resistance are controlled by adjusting the deposited volume of Ag NWs. Wrist bending and a wrist pulse are detected by the SS driven with the stored energy of the MSC. This work demonstrates the possible application of our TS MSC to future skin-attachable electronics as an integrated energy storage device.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
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•We introduce a new strategy for fabricating a fully self-healing all-in-one device.•PVA/borax/agarose/NaNO3 hydrogel electrolyte is synthesized.•Gold nanosheets (AuNSs) current ...collector for supercapacitor electrode is fabricated.•Flexible and self-healing planar supercapacitor can be achieved.•Multifunctional sensor can be operated by using the energy in the supercapacitor.•All device could retain their performance after repetitive self-healing.
We report on the fabrication of a rationally designed self-healing and flexible all-in-one system. Using a vacuum filtration method, an integrated system consisting of a planar supercapacitor and a multifunctional sensor is fabricated with gold nanosheet (AuNS) interconnections. A fast and reversible self-healing hydrogel based on the diol-ester bonding between poly(vinyl alcohol) (PVA) and borax is synthesized, and its mechanical stability is enhanced with the additional hydrogen bonding between PVA and agarose. Using an electrolyte of PVA/borax/agarose/NaNO3 and an electrode consisting of multiwalled carbon nanotubes (MWCNTs) coated on an AuNS current collector, a high-performance self-healing flexible planar supercapacitor is fabricated. The multifunctional sensor is made of MWCNTs with a zinc oxide nanowire composite. Both the supercapacitor and sensor recover their performance from bisectional damage via physical contact with a water supply. The self-healing supercapacitor shows mechanical stability for bending deformation even after repetitive self-healing cycles. Furthermore, the integrated system exhibits self-healing performance through stable sensing of NO2 gas and UV light using the stored energy in the supercapacitor after recovery from repetitive bisection of the sensor. This work demonstrates the high potential of our rationally designed flexible self-healing all-in-one system in high-performance wearable devices with high durability and longevity.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
A stretchable multisensor system is successfully demonstrated with an integrated energy‐storage device, an array of microsupercapacitors that can be repeatedly charged via a wireless radio‐frequency ...power receiver on the same stretchable polymer substrate. The integrated devices are interconnected by a liquid‐metal interconnection and operate stably without noticeable performance degradation under strain due to the skin attachment, and a uniaxial strain up to 50%.
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BFBNIB, FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SAZU, SBCE, SBMB, UL, UM, UPUK
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