Intriguingly, ATG5‐ATG12 positively regulated antiviral NF‐κB and IRF3 signalling during foot‐and‐mouth disease virus infection. ...Mauthe et al conduct an unbiased RNA interference screen approach ...to explore unconventional functions of ATG proteins during viral infections. In order to identify possible roles for ATG13 in antiviral immunity, we transfected human embryonic kidney cells HEK293T (293T cells) with an IFN‐sensitive response element (ISRE) luciferase reporter, which was an interferon regulatory factor 3 (IRF3)‐dependent promoter, and the internal control renilla luciferase, as well as expression vectors containing ATG13. The overexpression of ATG13 with the retinoic acid‐inducible gene‐I (RIG‐I)‐like receptors (RLRs) signalling proteins, including RIG‐I, melanoma differentiation‐associated antigen 5 (MDA5), mitochondrial antiviral signalling (MAVS), tumor necrosis factor (TNF) receptor associated factor (TRAF) family member associated NF‐κB activator (TANK) binding kinase 1 (TBK1), inhibitor of NF‐κB kinase subunit epsilon (IKKi), IRF3 and stimulator of interferon genes, additively activated ISRE luciferase activity (Figure B). ...we identified that ATG13 could interact with TRAF3 and TRAF6 by co‐immunoprecipitation assay (Figure L).
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FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SAZU, SBCE, SBMB, UL, UM, UPUK
Knoxia roxburghii (Spreng.) M. A. Rau (KR) is a plant clinically used in traditional Chinese medicine (TCM) for the treatment of cancer. The study objectives were to examine the effects of KR ...extracts, petroleum ether (PET), ethyl acetate (EtoAc), butanol (n-BuOH), and H2O-soluble fractions (HSF) of the 75% EtOH extraction on A549 (non-small cell lung cancer), HepG2 (liver cancer), HeLa (cervical cancer), MCF-7 (breast cancer), and L02 (normal hepatocyte) cells. It was found that HSF exhibited the strongest cytotoxic activity against MCF-7 cells, and was accompanied by reduced mitochondrial transmembrane potential, increased levels of intra-cellular reactive oxygen species (ROS) and activated caspases, and upregulated pro-apoptotic and downregulated anti-apoptotic proteins. LC-MS analysis further showed that HSF primarily consisted of calycosin, aloe emodin, rein, maackiain, asperuloside, orientin, vicenin-2, and kaempferide, which have been mostly reported for anti-tumor activity in previous studies. In summary, the current study illustrated the effect, mechanism, and the potential major active components of KR against breast cancer.
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IZUM, KILJ, NUK, PILJ, PNG, SAZU, UL, UM, UPUK
Nondoped organic light‐emitting diodes (OLEDs) have drawn enormous attention for their merits of process simplicity, reduced fabrication cost, and phase stability. Herein, a novel approach of ...utilizing intermolecular hydrogen bonding for enhancing performance of nondoped OLEDs with a new thermally activated delayed fluorescence (TADF) emitter 10‐(4‐(2,6‐di(pyrimidin‐5‐yl)pyridin‐4‐yl)phenyl)‐10H‐phenoxazine (DPmP‐PXZ) is investigated. Endowing with suitable intermolecular hydrogen bonds linking the ends of neighboring DPmP‐PXZ molecules, the molecules tend to form extended 1D molecular chain in solid. This 1D structure effectively keeps the electron‐rich PXZ cores in neighboring molecules apart from each other such that triplet‐related exciton quenching can be well suppressed. In addition, it also improves the balance of carrier mobilities and the optical out‐coupling from the emitting layer. With these merits, OLEDs using DPmP‐PXZ as a dopant emitter, from 10 to 100 wt%, can maintain high external quantum efficiencies (EQEs) of over 20%. More importantly, its nondoped OLED shows a yellow emission and an excellent maximum EQE of 21.8% with little efficiency roll‐off which is even comparable with state‐of‐the‐art yellow OLEDs. These results provide new insight on the role of hydrogen bonding in molecular packing and its subsequent influences on the performance of OLEDs.
Suitable intermolecular hydrogen bonding interactions of 10‐(4‐(2,6‐di(pyrimidin‐5‐yl)pyridin‐4‐yl)phenyl)‐10H‐phenoxazine (DPmP‐PXZ) enable the formation of 1D directional supramolecular extension, which not only effectively restricts the triplet‐related exciton quenching, but also balances the carrier mobilities and favors high out‐coupling efficiency. Thus, the organic light‐emitting diodes (OLEDs) using DPmP‐PXZ as the dopant maintain high external quantum efficiencies (EQEs) of over 20% with neglectable concentration quenching effect.
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BFBNIB, FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SAZU, SBCE, SBMB, UL, UM, UPUK
Thermally activated delayed fluorescence (TADF) emitters with aggregation-induced emission (AIE) features are hot candidates for non-doped organic light-emitting diodes (OLEDs), as they are highly ...emissive in solid states upon photoexcitation. Nevertheless, not every AIE-TADF emitter in the past had guaranteed decent efficiencies in non-doped devices, indicating that the AIE character alone does not necessarily afford ideal non-doped TADF emitters. As intermolecular electron-exchange interaction that involves long-lived triplet excitons plays a dominant role in the whole quenching process of TADF, we anticipate that it is the main reason for the different electroluminescence performances of AIE-TADF emitters. Therefore, in this work, we designed two TADF emitters SPBP-DPAC and SPBP-SPAC by modifying a reported less successful emitter BP-DPAC with extra fluorenes to increase intermolecular distances and attenuate this electron-exchange interaction. With the fluorene lock as steric hindrance, SPBP-DPAC and SPBP-SPAC exhibit significantly higher exciton utilization in non-doped films due to the suppressed concentration quenching. The non-doped OLEDs based on SPBP-DPAC and SPBP-SPAC show an excellent maximum external quantum efficiency (EQE) of 22.8% and 21.3% respectively, and what's even more promising is that ignorable roll-offs at practical brightness (
e.g.
, 1000 and 5000 cd m
−2
) were realized. These results reveal that locking the phenyl rings as steric hindrance can not only enhance the molecular rigidity, but also cause immediate relief of concentration quenching, and result in significant performance improvement under non-doped conditions. Our approach proposes a feasible molecular modification strategy for AIE-TADF emitters, potentially increasing their applicability in OLEDs.
Two TADF emitters were developed by modifying a reported less successful emitter BP-DPAC with fluorene to suppress concentration quenching. Their non-doped OLEDs displayed excellent EQEs of 22.8% and 21.3% with well-suppressed roll-off.
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IJS, KILJ, NUK, UL, UM, UPUK
Itch is an annoying sensation consisting of both sensory and emotional components. It is known to involve the parabrachial nucleus (PBN), but the following transmission nodes remain elusive. The ...present study identified that the PBN-central medial thalamic nucleus (CM)-medial prefrontal cortex (mPFC) pathway is essential for itch signal transmission at the supraspinal level in male mice. Chemogenetic inhibition of the CM-mPFC pathway attenuates scratching behavior or chronic itch-related affective responses. CM input to mPFC pyramidal neurons is enhanced in acute and chronic itch models. Specifically chronic itch stimuli also alter mPFC interneuron involvement, resulting in enhanced feedforward inhibition and a distorted excitatory/inhibitory balance in mPFC pyramidal neurons. The present work underscores CM as a transmit node of the itch signal in the thalamus, which is dynamically engaged in both the sensory and affective dimensions of itch with different stimulus salience.
Breast cancer is one of the most common cancer with high morbidity and mortality in women. This study aimed to explore the potential mechanism of costunolide inducing MCF‐7 cells apoptosis by ...multi‐spectroscopy, molecular docking, and cell experiments. The results manifested that costunolide interacted with calf thymus DNA (ct‐DNA) in a spontaneous manner, and the minor groove as the preferential binding mode. Furthermore, costunolide inhibited cell proliferation and colony formation. Hoechst 33258 staining showed that cell apoptosis induced by costunolide might be related to DNA damage. The apoptosis mechanism relied on regulating the protein expression of Bax, Bcl‐2, p53, Caspase‐3 and the activation of p38MAPK and nuclear factor κB (NF‐κB) pathways. This study will provide some experimental basis and potential therapeutic strategy for breast cancer treatment.
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BFBNIB, FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SAZU, SBCE, SBMB, UL, UM, UPUK
Abstract
Resolvin D2 (RvD2), an endogenous lipid mediator derived from docosahexaenoic acid, has been demonstrated to have analgesic effects. However, little is known about the mechanism underlying ...RvD2 in pain relief. Herein, we demonstrate that RvD2 targeted the P2X3 receptor as an analgesic. The electrophysiological activity of P2X3 receptors was suppressed by RvD2 in rat dorsal root ganglia (DRG) neurons. RvD2 pre‐application dose‐dependently decreased α,β‐methylene‐ATP (α,β‐meATP)‐induced inward currents. RvD2 remarkably decreased the maximum response to α,β‐meATP, without influencing the affinity of P2X3 receptors. RvD2 also voltage‐independently suppressed ATP currents. An antagonist of the G protein receptor 18 (GPR18), O‐1918, prevented the RvD2‐induced suppression of ATP currents. Additionally, intracellular dialysis of the Gα
i/o
‐protein antagonist pertussis toxin (PTX), the PKA antagonist H89, or the cAMP analog 8‐Br‐cAMP also blocked the RvD2‐induced suppression. Furthermore, α,β‐meATP‐triggered depolarization of membrane potential along with the action potential bursts in DRG neurons were inhibited by RvD2. Lastly, RvD2 attenuated spontaneous nociceptive behaviors as well as mechanical allodynia produced by α,β‐meATP in rats via the activation of the peripheral GPR18. These findings indicated that RvD2 inhibited P2X3 receptors in rat primary sensory neurons through GPR18, PTX‐sensitive Gα
i/o
‐proteins, and intracellular cAMP/PKA signaling, revealing a novel mechanism that underlies its analgesic effects by targeting P2X3 receptors.
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BFBNIB, FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SAZU, SBCE, SBMB, UL, UM, UPUK
Two novel thermally activated delayed fluorescence (TADF) emitters, 3-phenylquinolino3,2,1-deacridine-5,9-dione (3-PhQAD) and 7-phenylquinolino3,2,1-deacridine-5,9-dione (7-PhQAD), were designed ...and synthesized based on a rigid quinolino3,2,1-deacridine-5,9-dione (QAD) framework. With the effective superimposed resonance effect from electron-deficient carbonyls and electron-rich nitrogen atom, both emitters realize significant TADF characteristics with small ΔE STs of 0.18 and 0.19 eV, respectively. And, molecular relaxations were dramatically suppressed for both emitters because of their conjugated structure. In the devices, 3-PhQAD realizes superior performance with a maximum external quantum efficiency (EQE) of 19.1% and a narrow full width at half-maximum (FWHM) of 44 nm, whereas a maximum EQE of 18.7% and an extremely narrow FWHM of 34 nm are realized for 7-PhQAD. These superior results reveal that apart from nitrogen and boron-aromatic systems, QAD framework can also act as a TADF matrix with effective resonance effect, and QAD derivatives are ideal candidates to develop TADF emitters with narrow FWHMs for practical applications.
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IJS, KILJ, NUK, PNG, UL, UM
Scheme 1. Schematic representation of the HIPPEs stabilized solely by SPI-PA complexes. SPI-PA complexes -stabilized HIPPEs with φ = 0.8.
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•A green facile method to construct SPI-PA ...complexes as stabilizer was reported.•A HIPPEs stabilized by SPI-PA complexes was successfully constructed.•The HIPPEs have outstanding emulsifying and antioxidative properties.
Protein-based high internal phase Pickering emulsions (HIPPEs) feature numerous multi-functionalities and widespread applications. However, the direct use of native proteins for the constructions of HIPPEs is limited since it is fragile under various conditions. Here, cold plasma was used to modify soy protein isolates (SPI) to improve their surficial properties. Meanwhile, proanthocyanidins (PA) were applied to interact with cold plasma-treated SPI to form complex. Furthermore, the well-prepared SPI-PA complex was used to construct novel HIPPEs. Results showed cold plasma treatment significantly improved the functionalities of SPI, which were confirmed by surface hydrophobicity (H0 ＜ 500), sulfhydryl (SH) groups and spectral analysis. Further, the emulsification and oxidation resistance of cold plasma treated SPI were enhanced after forming complex with PA. Soybean oils can be stabilized by SPI-PA complexes to form HIPPEs with a lipid oxidation inhibition rate of ＞ 65%, creaming index (CI) ＞ 80%, excellent rheological properties and better stability compared with conventional emulsion systems. Overall, this SPI-PA complexes provides a unique approach to improve the emulsification and oxidation resistance to engineer HIPPEs with versatile applications.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
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