Summary
In angiosperms, the timely delivery of sperm cell nuclei by pollen tube (PT) to the ovule is vital for double fertilization. Penetration of PT into maternal stigma tissue is a critical step ...for sperm cell nuclei delivery, yet little is known about the process. Here, a male‐specific and sporophytic mutant xt6, where PTs are able to germinate but unable to penetrate the stigma tissue, is reported in Oryza sativa. Through genetic study, the causative gene was identified as Chalcone synthase (OsCHS1), encoding the first enzyme in flavonoid biosynthesis. Indeed, flavonols were undetected in mutant pollen grains and PTs, indicating that the mutation abolished flavonoid biosynthesis. Nevertheless, the phenotype cannot be rescued by exogenous application of quercetin and kaempferol as reported in maize and petunia, suggesting a different mechanism exists in rice. Further analysis showed that loss of OsCHS1 function disrupted the homeostasis of flavonoid and triterpenoid metabolism and led to the accumulation of triterpenoid, which inhibits significantly α‐amylase activity, amyloplast hydrolysis and monosaccharide content in xt6, these ultimately impaired tricarboxylic acid (TCA) cycle, reduced ATP content and lowered the turgor pressure as well. Our findings reveal a new mechanism that OsCHS1 modulates starch hydrolysis and glycometabolism through modulating the metabolic homeostasis of flavonoids and triterpenoids which affects α‐amylase activity to maintain PT penetration in rice, which contributes to a better understanding of the function of CHS1 in crop fertility and breeding.
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BFBNIB, DOBA, FZAB, GIS, IJS, IZUM, KILJ, NLZOH, NUK, OILJ, PILJ, PNG, SAZU, SBCE, SBMB, UILJ, UKNU, UL, UM, UPUK
We report the results of our systematic survey for Galactic 6.7 GHz Class II CH3OH maser emission toward a sample of young stellar objects. The survey was conducted with the Shanghai Tianma Radio ...Telescope (TMRT). The sample consists of 3348 sources selected from the all-sky Wide-field Infrared Survey Explorer (WISE) point-source catalog. We discussed the selection criteria in detail and the detection results of those at high Galactic latitudes (i.e., ) in a previous paper (Yang et al. 2017). Here, we present the results from the survey of those at low Galactic latitudes, i.e., . Of 1875 selected WISE point sources, 291 positions that were actually associated with 224 sources that were detected with CH3OH maser emission. Among them, 32 are newly detected. A majority of the newly detected sources are associated with bright WISE sources. The majority of the detected sources (209/224 = 93.3%) are quite close to the Galactic Plane ( ) and lie on the inner spiral arms with positive local standard of rest velocities. The detection rate and the color-color distribution of our detection are all matched with our anticipation. Combining with detections from previous surveys, we compile a catalog of 1085 sources with 6.7 GHz CH3OH maser emission in our Galaxy.
Compared with the widely used constant switching frequency pulse-width-modulation (PWM) method, variable switching frequency PWM can benefit more because of the extra freedom. Based on the analytical ...expression of current ripple of three-phase converters, variable switching frequency control methods are proposed to satisfy different ripple requirements. Switching cycle T_{s} is updated in DSP in every interruption period based on the ripple requirement. Two methods are discussed in this paper. The first method is designed to arrange the current ripple peak value within a certain value and can reduce the equivalent switching frequency and electromagnetic interference (EMI) noise; the second method is designed to keep ripple current RMS value constant and reduce the EMI noise. Simulation and experimental results show that variable switching frequency control could improve the performance of EMI and efficiency without impairing the power quality.
Circular RNAs (circRNAs) have been shown to interact with microRNAs (miRNA) as competitive endogenous RNAs (ceRNAs) to regulate target gene expression and participate in tumorigenesis. However, the ...role of circRNA-mediated ceRNAs in bladder cancer (BC) remains unknown. Accordingly, the aim of this study was to elucidate the regulatory mechanisms in BC based on construction of the ceRNA network.
The RNA expression profiles were obtained from public datasets in the Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) database, and were used to establish a circRNA-miRNA-mRNA network. The interactions among proteins were analyzed using the STRING database and hubgenes were extracted using the cytoHubba application. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses of differentially expressed mRNAs in BC and normal tissue samples were performed to determine the functions of the intersecting mRNAs.
A total of 27 circRNAs, 76 miRNAs, and 4744 mRNAs were found to be differentially expressed between BC and normal tissues. The circRNA-miRNA-mRNA ceRNA network was established based on 21 circRNAs, 14 miRNAs, and 150 mRNAs differentially expressed in BC. We also established a protein-protein interaction network and identified 10 hubgenes, which were used to construct circRNA-miRNA-hubgene regulatory modules. The most enriched biological process GO term was strand displacement (P<0.05), and the homologous recombination and Fanconi anemia pathways were significantly enriched (P<0.05) for the differentially expressed genes in BC.
We screened several dysregulated circRNAs and established a circRNA-associated ceRNA network by bioinformatics analysis. The identified ceRNAs are likely critical in the pathogenesis of BC and may serve as future therapeutic biomarkers.
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DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Electrocatalytic reduction of carbon dioxide to valuable chemicals is a sustainable technology that can achieve a carbon‐neutral energy cycle in the environment. Electrochemical CO2 reduction ...reaction (CO2RR) processes using metal–organic frameworks (MOFs), featuring atomically dispersed active sites, large surface area, high porosity, controllable morphology, and remarkable tunability, have attracted considerable research attention. Well‐defined MOFs can be constructed to improve conductivity, introduce active centers, and form carbon‐based single‐atom catalysts (SACs) with enhanced active sites that are accessible for the development of CO2 conversion. In this review, the progress on pristine MOFs, MOF hybrids, and MOF‐derived carbon‐based SACs is summarized for the electrocatalytic reduction of CO2. Finally, the limitations and potential improvement directions with respect to the advancement of MOF‐related materials for the field of research are discussed. These summaries are expected to provide inspiration on reasonable design to develop stable and high‐efficiency MOFs‐based electrocatalysts for CO2RR.
With many advantages including atomically dispersed active sites and controllable structures, metal–organic frameworks (MOFs)‐related single‐atom catalysts have been identified as promising catalysts for the electrochemical CO2 reduction. The progress on MOF‐related single‐atom catalysts for CO2 reduction is highlighted. The challenges and potential improvement directions concerning the advancement of MOF‐related materials for CO2 reduction are proposed.
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BFBNIB, FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SBCE, SBMB, UL, UM, UPUK
The three-phase pulsewidth-modulation (PWM) converter is one of the most widely used topologies for power conversion. In order to design PWM methods, the influence of PWM methods on the current ...ripple is needed. This paper studies the current ripple of a three-phase PWM converter with general PWM methods for the design and control of this kind of converter. The current ripple is analyzed with eight different Thevenin equivalent circuits for the eight different voltage vectors. Then, the current-ripple slope and effective time could be achieved for every period. The current ripple could be predicted with both peak and rms values. Analytical predicted results show that discontinuous PWM could generate obviously bigger current ripples than space vector PMW for both peak and rms values with the same conditions. Simulation and experiments are built to verify the analytical results, proving that the theoretical prediction is valid. This analysis provides the basis for the design and control of the PWM method for converters.
This paper introduces series work of common-mode (CM) voltage reduction for the paralleled inverters. The paralleled inverters' phase-legs are connected through coupling inductors and the combined ...three-phase currents are provided to the load. Interleaving is an approach to reduce the CM voltage for the paralleled inverters but it cannot eliminate CM voltage. A novel pulse-width-modulation (PWM) method for paralleled inverters which can theoretically achieve zero CM voltage is developed. Considering the basic voltage vectors in each inverter, novel paralleled voltage vectors which have zero CM voltage are proposed to combine the reference voltage vector. The action time's distribution and voltage vectors' sending sequence for each inverter are also introduced. The proposed PWM method can make sure the voltage of the two inverters are balanced in each switching cycle and limits the circulating current through small coupling inductors. Similar to interleaving space vector PWM, the proposed zero CM PWM also has the ability to reduce the output current ripple and electromagnetic interference (EMI). Simulation and experimental results are provided to show the advantage of paralleled inverters in CM voltage reduction and validate the proposed method has good performance to reduce CM current and CM EMI noise.
Previous genome-wide association studies have found two single nucleotide polymorphisms (SNP) rs7692387 and rs1842896 located on or near the GUCY1A3 gene were associated with coronary artery disease ...(CAD). GUCY1A3 was considered to be involved in the process of atherosclerosis, but there was little information about the association between genotypic polymorphisms of the GUCY1A3 and large artery atherosclerotic (LAA) stroke. This study aimed to investigate the associations between the GUCY1A3 rs7692387, rs1842896 polymorphisms and LAA stroke susceptibility.
A total of 298 LAA stroke patients and 300 control subjects from a southern Chinese Han population were included. SNaPshot technique was used for genotype analysis. Associations between genotypes and LAA stroke susceptibility were analyzed with logistic regression model.
Our study found that under the recessive model (TT vs. GT + GG), the GUCY1A3 rs1842896 polymorphism was significantly correlated with LAA stroke (OR = 1.48, 95%CI: 1.07-2.04, P = 0.018). After adjustment for its effects on age, gender, cigarette smoking, total cholesterol, low-density lipoprotein cholesterol, HbA1c, hypertension, diabetes mellitus, and CAD, the rs1842896 TT genotype retained association with increased susceptibility to LAA stroke (recessive model: adjusted OR = 1.96, 95%CI: 1.22-3.17, P = 0.006). However, association between rs7692387 polymorphism with LAA stroke was not observed.
Our results indicate that the GUCY1A3 rs1842896 polymorphism is an LAA stroke risk factor in Southern Han Chinese.
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DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Poly(vinylidene fluoride) was grafted with maleic anhydride through reactive extrusion by using diisopropyl benzene peroxide as an initiator and 9-vinyl anthracene as a stabilizer. Effects of various ...parameters on grafting degree were investigated including the amounts of monomer, initiator and stabilizer. The maximum extent of grafting achieved was 0.74%. The graft polymers were characterized using FTIR, water contact angle, thermal, mechanical and XRD studies. Improved hydrophilic and mechanical properties were observed for graft polymers.
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IZUM, KILJ, NUK, PILJ, PNG, SAZU, UL, UM, UPUK
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