A rare case of psoriasiform graft-versus-host disease is reported. The patient suffered from acute B-cell leukemia for more than 1 year. Nine months after allogenic hematopoietic stem cell ...transplantation, the patient developed red papules and plaques, with Auspitz sign positive and silver-white scales on the head, face, limb and trunk. The histopathology showed both psoriatic and interfacial dermatitis. Immunohistochemical staining demonstrated infiltration of CD4+ and CD8+ T cells. Diagnosis was psoriasiform graft-versus-host disease. Lesions were improved by topical 0.1% tacrolimus ointment.
A physical model is proposed to study the heat transfer and oil flow of a buried hot oil pipeline under normal operation. With certain physically reasonable assumptions, the governing equations for ...the thermal analyses are derived. An approach combining unstructured-finite-volume
1 and finite difference methods is applied to solve the governing equations, in which the soil domain was discretized by unstructured grids. Numerical simulations in a wide range of operating conditions are conducted. The operating conditions cover 5 months (April, May, June, October and November) with throughputs ranging from 15,007 tons per day to 27,451 tons per day and outlet temperatures varying from 40.6 °C to 64.8 °C. Measured data are provided for comparison. A good agreement between numerical simulations and field measurement suggests that the proposed numerical scheme is a suitable method to simulate the heat transfer and oil flow of buried hot crude oil pipelines. We also analyze a number of influential factors on the temperature distribution of oil along the pipeline.
► Advanced numerical methods applied based on the appropriate models. ► Satisfactory results obtained via the software verified by field data. ► Practical research achievements provide reference to pipeline operation.
Full text
Available for:
GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK
•A new method to predict and monitor concrete strength development.•The method adopts the state-of-the-art embedded smart aggregates, electromechanical impedance technique, and machine ...learning.•Conductance resonant frequency (CRF) and conductance resonant peak (CRP) are good concrete strength indicators.•The combined model with linear regression and convolutional neural network, has achieved the highest prediction accuracy.
It is crucial to monitor the real-time strength development of early-age concrete, which is related to the structure safety and construction efficiency of concrete. This paper proposed a new method to predict and monitor concrete strength development. The basic idea is using embedded smart aggregates (SAs), combining the electromechanical impedance (EMI) technology and machine learning to monitor the strength development of early-age concrete. An EMI model of the embedded SA was firstly established and the relationship between the conductance resonant frequency (CRF) and conductance resonant peak (CRP) of the SA and the development of concrete strength was investigated. The conductance signatures were continuously monitored and the compressive strength of the specimens at different ages was tested. Hence, the EMI model results that the CRF decreases but CRP increases as the compressive strength of concrete increases were verified by analyzing the collected data. The four different models of a linear regression model (LR), a convolutional neural network (CNN), a hybrid (LR-CNN) model, and an empirical equation were established. The conductance of SAs as the developed models inputs, the compressive strength of specimens as outputs. Finally, these four models were compared with each other. Meanwhile, the performance indicators of the LR-CNN model and other hybrid models were compared. The results show that the LR-CNN model has excellent performance and successfully achieves quantitative prediction of concrete strength development. The proposed method for evaluating and predicting the compressive strength of concrete is simple, accurate, quantitative and reliable, and has promising application.
Full text
Available for:
GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
The dimorphic fungus Talaromyces marneffei (TM) is a common cause of HIV-associated opportunistic infections in Southeast Asia. Cotrimoxazole (CTX) inhibits folic acid synthesis which is important ...for the survival of many bacteria, protozoa, and fungi and has been used to prevent several opportunistic infections among HIV/AIDS patients. We question whether CTX is effective in preventing TM infection. To investigate this question, we conducted an 11-year (2005-2016) retrospective observational cohort study of all patients on the Chinese national antiretroviral therapy (ART) programme in Guangxi, a province with high HIV and TM burden in China. Survival analysis was conducted to investigate TM cumulative incidence, and Cox regression and propensity score matching (PSM) were used to evaluate the effect of CTX on TM incidence. Of the 3359 eligible individuals contributing 10,504.66 person-years of follow-up, 81.81% received CTX within 6 months after ART initiation, and 4.73% developed TM infection, contributing 15.14/1,000 person-year TM incidence rate. CTX patients had a significantly lower incidence of TM infection than non-CTX patients (4.11% vs. 7.53%; adjusted hazard ratio (aHR) = 0.50, 95% CI 0.35-0.73). CTX reduced TM incidence in all CD4
+
cell subgroups (<50 cells/μL, 50-99 cells/μL, 100-199 cells/μL), with the highest reduction observed in patients with a baseline CD4
+
cell count <50 cells/μL in both Cox regression and the PSM analyses. In conclusion, in addition to preventing other HIV-associated opportunistic infections, CTX prophylaxis has the potential to prevent TM infection in HIV/AIDS patients receiving ART.
Fluoride anion plays an important role in dental health and the treatment of osteoporosis. On the other hand, over exposure to fluoride may lead to severely diseases. Therefore, quantitative analysis ...of fluoride ions with high specificity and sensitivity by facile and rapid method is in urgent demand. In this paper, an amino-group functionalized MOFs NH2-UiO-66 is synthesized by treating Zr4+ ions and 2-aminaterephthalic acid under hydrothermal condition. A drastic fluorescence enhancement of NH2-UiO-66 at 455 nm induced by fluoride ions is demonstrated. Using NH2-UiO-66 as a receptor, a high selective and sensitive fluorescent detection of F- ions in aqueous solution is achieved. This detection is free from interferences of the co-existing anions and cations, such as Cl-, Br- and I-, NO3-, SO42-, PO43-, CO32-, metal ions and humic acid, etc, for which the allowed amount is up to 1.0 g L−1. The detection limit of F- anion is 0.229 mg L−1. The F- anion-enhanced fluorescent sensing mechanism can be ascribed to the formation of hydrogen bonds between the guest F- anions and the free amino groups in NH2-UiO-66, which result in incremental electron transfer from organic ligands to Zr-oxo clusters. These studies indicate that the luminescent MOF material of NH2-UiO-66 as fluorescent probes may hold promising application in highly selective and sensitive detection of F- anions together with facile receptor fabrication and regeneration.
Full text
Available for:
GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK
Compared to terrestrial environments, the oceans harbor a variety of environments, creating higher biodiversity, which gives marine natural products a high occurrence of significant biology and novel ...chemistry. However, traditional bioassay-guided isolation and purification strategies are severely limiting the discovery of additional novel natural products from the ocean. With an increasing number of marine microorganisms being sequenced, genome mining is gradually becoming a powerful tool to retrieve novel marine natural products. In this review, we have summarized genome mining approaches used to analyze key enzymes of biosynthetic pathways and predict the chemical structure of new gene clusters by introducing successful stories that used genome mining strategy to identify new marine-derived compounds. Furthermore, we also put forward challenges for genome mining techniques and their proposed solutions. The detailed analysis of the genome mining strategy will help researchers to understand this novel technique and its application. With the development of a genome sequence, genome mining strategies will be applied more widely, which will drive rapid development in the field of marine natural product development.
Full text
Available for:
BFBNIB, DOBA, GIS, IJS, IZUM, KILJ, KISLJ, NUK, PILJ, PNG, SAZU, UILJ, UKNU, UL, UM, UPUK
Alginate lyases with unique characteristics for degrading alginate into size-defined oligosaccharide fractions, were considered as the potential agents for disrupting Pseudomonas aeruginosa biofilms. ...In our study, a novel endolytic PL-7 alginate lyase, named AlyG2, was cloned and expressed through Escherichia coli. This enzyme exhibited excellent properties: it maintained more than 85% activity at low temperatures of 4 °C and high temperatures of 70 °C. After 1 h of incubation at 4 °C, it still retained over 95% activity, demonstrating the ability to withstand low temperature. The acid-base and salt tolerance properties shown it preserves more than 50% activity in the pH range of 5.0 to 11.0 and in a high salt environment at 3000 mM NacCl, indicating its high stability in several aspects. More importantly, AlyG2 in our research was revealed to be effective at removing mature biofilms and inhibiting biofilm formation produced by Pseudomonas aeruginosa, and the inhibition and disruption rates were 47.25 ± 4.52% and 26.5 ± 6.72%, respectively. Additionally, the enzyme AlyG2 promoted biofilm disruption in combination with antibiotics, particularly manifesting the synergistic effect with erythromycin (FIC=0.5). In all, these results offered that AlyG2 with unique characteristics may be an effective technique for the clearance or disruption of biofilm produced by P. aeruginosa.
Display omitted
•A novel PL-7 family (subfamily 6) alginate lyase was characterized.•AlyG2 shown high stability in temperature, various metal ions and wide pH conditions.•AlyG2 was a bifunctional endolytic enzyme and mainly active on polyM.•AlyG2 shown anti-biofilm effect on Pseudomonas aeruginosa and synergistic effect with EM.
Full text
Available for:
GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
PDF
