Provider: - Institution: - Data provided by Europeana Collections- München, Bayerische Staatsbibliothek -- 2 Oecon. 110- All metadata published by Europeana are available free of restriction under ...the Creative Commons CC0 1.0 Universal Public Domain Dedication. However, Europeana requests that you actively acknowledge and give attribution to all metadata sources including Europeana
Provider: Manuscriptorium - National Library of the Czech Republic - Institution: - Data provided by Europeana Collections- Beytrag zur teutschen holzgerechten Forstwissenschaft, die Anpflanzung ...Nordamericanischer Holzarten, mit Anwendung auf teutsche Forste, betreffend /- Údaje o autorovi dle CERLu- All metadata published by Europeana are available free of restriction under the Creative Commons CC0 1.0 Universal Public Domain Dedication. However, Europeana requests that you actively acknowledge and give attribution to all metadata sources including Europeana
Bildnis des Carl Weishaupt Adam, Julius; Kunst- und Landkartenhandlung Mey und Widmayer <München>
1845/1855
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New regulatory roles continue to emerge for both natural and engineered noncoding RNAs, many of which have specific secondary and tertiary structures essential to their function. Thus there is a ...growing need to develop technologies that enable rapid characterization of structural features within complex RNA populations. We have developed a high-throughput technique, SHAPE-Seq, that can simultaneously measure quantitative, single nucleotide-resolution secondary and tertiary structural information for hundreds of RNA molecules of arbitrary sequence. SHAPE-Seq combines selective 2'-hydroxyl acylation analyzed by primer extension (SHAPE) chemistry with multiplexed paired-end deep sequencing of primer extension products. This generates millions of sequencing reads, which are then analyzed using a fully automated data analysis pipeline, based on a rigorous maximum likelihood model of the SHAPE-Seq experiment. We demonstrate the ability of SHAPE-Seq to accurately infer secondary and tertiary structural information, detect subtle conformational changes due to single nucleotide point mutations, and simultaneously measure the structures of a complex pool of different RNA molecules. SHAPE-Seq thus represents a powerful step toward making the study of RNA secondary and tertiary structures high throughput and accessible to a wide array of scientific pursuits, from fundamental biological investigations to engineering RNA for synthetic biological systems.
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The widespread natural ability of RNA to sense small molecules and regulate genes has become an important tool for synthetic biology in applications as diverse as environmental sensing and metabolic ...engineering. Previous work in RNA synthetic biology has engineered RNA mechanisms that independently regulate multiple targets and integrate regulatory signals. However, intracellular regulatory networks built with these systems have required proteins to propagate regulatory signals. In this work, we remove this requirement and expand the RNA synthetic biology toolkit by engineering three unique features of the plasmid pT181 antisense-RNA-mediated transcription attenuation mechanism. First, because the antisense RNA mechanism relies on RNA-RNA interactions, we show how the specificity of the natural system can be engineered to create variants that independently regulate multiple targets in the same cell. Second, because the pT181 mechanism controls transcription, we show how independently acting variants can be configured in tandem to integrate regulatory signals and perform genetic logic. Finally, because both the input and output of the attenuator is RNA, we show how these variants can be configured to directly propagate RNA regulatory signals by constructing an RNA-meditated transcriptional cascade. The combination of these three features within a single RNA-based regulatory mechanism has the potential to simplify the design and construction of genetic networks by directly propagating signals as RNA molecules.
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Advances in biosensor engineering have enabled the design of programmable molecular systems to detect a range of pathogens, nucleic acids, and chemicals. Here, we engineer and field-test a biosensor ...for fluoride, a major groundwater contaminant of global concern. The sensor consists of a cell-free system containing a DNA template that encodes a fluoride-responsive riboswitch regulating genes that produce a fluorescent or colorimetric output. Individual reactions can be lyophilized for long-term storage and detect fluoride at levels above 2 ppm, the Environmental Protection Agency’s most stringent regulatory standard, in both laboratory and field conditions. Through onsite detection of fluoride in a real-world water source, this work provides a critical proof-of-principle for the future engineering of riboswitches and other biosensors to address challenges for global health and the environment.
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Provider: - Institution: Ghent University Library - Data provided by Europeana Collections- Bijeengezameld, gerangschikt en uitgeg. door hunnen vriend Baron C. F. Sirtema van Grovestins.- All ...metadata published by Europeana are available free of restriction under the Creative Commons CC0 1.0 Universal Public Domain Dedication. However, Europeana requests that you actively acknowledge and give attribution to all metadata sources including Europeana
The expansion of technostress research in the organisational and private IS usage contexts has generated substantial theoretical and empirical insights into the relationship between technostress ...creators and psychological and behavioural outcomes. However, we observe empirical inconsistencies in terms of effect sizes and conceptual inconsistencies regarding the aggregated and disaggregated treatment of technostress creators. Against this background, we argue that a fine-grained estimation and comparison of effect size strengths of technostress creators on outcomes can provide clarity on these essential matters. Using the Hunter and Schmidt method, we integrated and synthesised empirical data from 102 articles, encompassing 113 independent studies with a total of 49,955 observations. Our analysis offers four important contributions to the technostress literature. First, it confirms that technostress is meaningful in terms of its detrimental impact on both psychological and behavioural outcomes. Second, the results provide accurate effect size estimates for technostress creators on different outcomes in organisational and private usage contexts. Third, the results reveal that psychological outcomes are more immediate than behavioural outcomes. Fourth, the findings suggest that in certain contexts, a disaggregated account of technostress creators can reveal meaningful empirical information.
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