The amount of circulating data on the internet has witnessed a considerable increase during the last decades. A web site is the main source that provides users' needs. However, some of the existing ...web sites are not well intentioned by users. Many studies have treated the problem of assessing the web sites' quality of different categories such as ecommerce, education, entertainment, health, etc. The problematic implies a multiple criteria decision making (MCDM) due to the multiple conflicting criteria for assessment. Existing methods are mainly based on making a hierarchy to divide high level criteria, sub-level criteria and alternatives. There is no standard until now that defines important criteria for evaluation. Indeed, this paper presents a process of collecting and extracting data from a list of studies according to a Systematic Literature Review (SLR) method. In fact, it is necessary to know frequent criteria used in the literature for establishing the task of assessment. This paper proposes also a determination of an association rules' set extracted from a set of criteria by applying an Apriori method.
Key Clinical MessageHydatidosis is a parasitic infection caused by the larval form of "Echinococcus granulosis." Bone localization is rare even in endemic areas. We report an unusual case of an ...extensive hydatidosis of the right sacrum and femur with muscle involvement. AbstractWe report the case of a man, with a history of visceral hydatidosis, who developed after 14 years an extensive hydatidosis of the sacrum and femur with muscle involvement. The treatment of visceral hydatidosis was chemotherapy with albendazole for a long term. Conservative surgical treatment is considered for bone locations.
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FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SAZU, SBCE, SBMB, UL, UM, UPUK
In this paper, we present a quality assessment method and model in order to measure the performance of dynamic institutional websites. This method called Fuzz-web is based on Multiple Criteria ...Decision Making. Obviously, a phase of selecting appropriate evaluation criteria is necessary for the decision making process. Since the evaluation process is characterized by subjectivity and imprecision, we attempt to address the problem using fuzzy logic as an intelligent technology. Some tests realized on a set of Tunisian and foreign websites allow us to discuss the proposed reduced fuzzy method and then to validate the decision making result.
Multiple Criteria Decision Making (MCDM) is widely used in everyday life especially to make decisions between conflicted criteria. In this paper, MCDM is explored in the Web domain that plays a major ...role in the modern society. A web site can provide or not users' needs. Assess the quality of web sites requires a list of criteria and sub-criteria, they depend also on web site category. So, to qualify criteria versus others it is important to resort to Multi-Criteria decision Making method. Weighing criteria, ranking web sites or other purposes of assessment can be resolved by MCDM. This paper identifies and discusses some methods and techniques in this area; it proposes also a comparative study between them and concludes with some findings discussions and future issues.
Background: As an oleaginous yeast, Yarrowia lipolytica is able to assimilate hydrophobic substrates. This led to the isolation of several promoters of key enzymes of this catabolic pathway. Less is ...known about the behavior of Y. lipolytica in large bioreactors using these substrates. There is therefore a lack of established know-how concerning high cell density culture protocols of this yeast. Consequently, the establishment of suitable induction conditions is required, to maximize recombinant protein production under the control of these promoters. Results: Human interferon a2b (huIFN a2b) production in Yarrowia lipolytica was used as a model for the enhancement of recombinant protein production under the control of the oleic acid (OA)-inducible promoter POX2. Cell viability and heterologous protein production were enhanced by exponential glucose feeding, to generate biomass before OA induction. The optimal biomass level before induction was determined (73 g L-1), and glucose was added with oleic acid during the induction phase. Several oleic acid feeding strategies were assessed. Continuous feeding with OA at a ratio of 0.02 g OA per g dry cell weight increased huIFNa2b production by a factor of 1.88 (425 mg L-1) and decreased the induction time (by a factor of 2.6, 21 h). huIFN a2b degradation by an aspartic protease secreted by Y. lipolytica was prevented by adding pepstatin (10 μM), leading to produce a 19- fold more active huIFN a2b (26.2 × 107 IU mg-1). Conclusion: Y. lipolytica, a generally regarded as safe (GRAS) microorganism is one of the most promising non conventional yeasts for the production of biologically active therapeutic proteins under the control of hydrophobic substrate-inducible promoter.
Background: As an oleaginous yeast, Yarrowia lipolytica is able to assimilate hydrophobic substrates. This led to the isolation of several promoters of key enzymes of this catabolic pathway. Less is ...known about the behavior of Y. lipolytica in large bioreactors using these substrates. There is therefore a lack of established know-how concerning high cell density culture protocols of this yeast. Consequently, the establishment of suitable induction conditions is required, to maximize recombinant protein production under the control of these promoters. Results: Human interferon a2b (huIFN a2b) production in Yarrowia lipolytica was used as a model for the enhancement of recombinant protein production under the control of the oleic acid (OA)-inducible promoter POX2. Cell viability and heterologous protein production were enhanced by exponential glucose feeding, to generate biomass before OA induction. The optimal biomass level before induction was determined (73 g L-1), and glucose was added with oleic acid during the induction phase. Several oleic acid feeding strategies were assessed. Continuous feeding with OA at a ratio of 0.02 g OA per g dry cell weight increased huIFNa2b production by a factor of 1.88 (425 mg L-1) and decreased the induction time (by a factor of 2.6, 21 h). huIFN a2b degradation by an aspartic protease secreted by Y. lipolytica was prevented by adding pepstatin (10 μM), leading to produce a 19- fold more active huIFN a2b (26.2 × 107 IU mg-1). Conclusion: Y. lipolytica, a generally regarded as safe (GRAS) microorganism is one of the most promising non conventional yeasts for the production of biologically active therapeutic proteins under the control of hydrophobic substrate-inducible promoter.
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