Abstract This study investigated some biological properties of Artemisia campestris aqueous extract (ACAE) as well its global chemical compositions. Twenty four rats were excised on the posterior ...neck skin area and divided into 4 groups, treated respectively with: sterile saline, glycerol, CICAFLORA and ACAE. The wound closure rate, histopathology evolution and the superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA) level in skin tissue were evaluated. Anti-inflammatory activity was studied by carrageenan-induced rat paw edema. Animals were divided into 3 groups pre-treated respectively with sterile saline, acetylsalicylic acid (AA) and ACAE. The antibacterial activity was tested against six bacteria and the antioxidant activity was estimated by the 1,1-diphenyl-2-picrylhydrazyl (DPPH), reducing power and β-carotene activities. Our results demonstrated a significant improvement in wound healing progression and in oxidative stress damage in the wounds tissues of ACAE-treated rats, compared to control. ACAE-treated rats revealed also a significant inhibition of carrageenan-induced hind paws edema as confirmed by the histological analysis. In addition to the antioxidant activity, ACAE showed considerable antibacterial activities. ACAE exhibited important wound healing effect probably due to the anti-inflammatory, antibacterial and antioxidant activities of its phytochemical contents. Therefore, this study confirms its popular use and highlights its promise in the development of new drugs.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK, ZRSKP
This study aimed to evaluate the potentiality of a mineral and antioxidant-rich methanolic extract of the red marine alga
(FRE) against methyl-thiophanate (MT)-induced toxicity in adult rats. The ...animals were allocated into four groups: controls, MT (300 mg/kg), MT + FRE, and FRE-treated group for 7 days. Our results demonstrated severe mineral perturbations due to MT treatment, especially in calcium and phosphorus levels in plasma, urine, and bone. Similarly, the hematological analysis revealed increased red blood cells, platelets, and white blood cells associated with striking genotoxicity. Interestingly, a significant rise in lipid peroxidation and advanced oxidation protein products level in erythrocytes and bone were noted. Meanwhile, a depletion of the antioxidant status in both tissues occurred. These biochemical alterations were in harmony with DNA degradation and histological variation in bone and blood. In the other trend, data showed that treatment with alga improved MT-induced hematotoxicity, genotoxicity, and oxidative stress in the blood and bone. Osteo-mineral metabolism and bone histo-architecture were also noted. In conclusion, these findings demonstrated that the red alga
is a potent source of antioxidant and antibacterial agents, as revealed by the in vitro analysis.
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IZUM, KILJ, NUK, PILJ, PNG, SAZU, UL, UM, UPUK
The present study aimed to evaluate the antioxidant effects of polysaccharide (PS) isolated from Balangu Shirazi (Lallemantia royleana) seeds in vitro as well as on the hypercholesterolemic ...diet-induced liver and kidney injury in adult rats. PS was structurally characterized by Fourier-transformed infrared, which confirmed the presence of bands characteristic of polysaccharides. Functional properties of PS were investigated based on water solubility index, holding and emulsifying capacities. The antioxidant activities were confirmed by DPPH radical scavenging assays, reducing power and the chelating effect assay. The administration of PS to a hypercholesterolemic diet for 30 days in Wistar rats significantly improved the liver and kidney levels in malondialdehyde, advanced oxidation protein products, glutathione, superoxide dismutase, glutathione peroxidase and in vitamin C. The oxidative stress profile was confirmed by hematological and plasma biochemical parameters. In addition, histological alterations were significantly ameliorated in liver and kidney tissues. The study strengthens the hypothesis that the herbal polysaccharide can be used as a novel antioxidant and hypocholesterolemic compound against hyperlipidemia-induced atherosclerosis.
The aerial part of Clematis flammula (Ranunculaceae) has been traditionally used in the treatment of skin diseases including mycotic infection in the Tunisian traditional medicine. The study was ...undertaken to extract and determine the essential oil chemical composition of Clematis flammula aerial parts and to assess the potential of anemonin in wound healing on mechanically wounded wistar rats. The essential oil was obtained by hydrodistillation and analyzed by GC-MS. Anemonin was isolated and then incorporated as active in a cream for which the cytotoxicity was evaluated by methyl thiazolyl tetrazolium (MTT)-based colorimetric assay. Then, its potential in wound healing on mechanically wounded wistar rats was assessed. The GC-MS analysis showed that the major compound was protoanemonin (86.74%) which spontaneously dimerised in part to form the anemonin. The wound healing activity of anemonin cream exhibited a non toxic potential of anemonin at a concentration of 25 µg/mL with a cell migration efficiency that reaches more than 80% after 48 hours of treatment. Wound healing efficiency was evaluated by monitoring morphological and skin histological analyses. Comparable wound surface reduction of the group treated by anemonin cream (p ≥ 0.05) when compared to the reference treated group. The skin histological analysis showed the completely wound closure. Antioxidant activity was assessed by the malondialdehyde (MDA) rates and antioxidant enzymes (glutathione peroxidase (GPx) and catalase) determination. The results provided strong support for the effective wound healing activity of anemonin cream, making it a promising candidate as a therapeutic agent in tissue repairing processes.
Muco‐cutaneous melanosis is a benign entity with no progression. Although, dermoscopic features may help to differentiate melanosis from malignant pigmented diseases, histopathology remains crucial ...for the confirming of melanosis of the nipple and areola. Herein, we represent a new case of melanosis of the areola and we describe its clinico‐pathological aspects.
Muco‐cutaneous melanosis is a benign entity with no progression. Although, dermoscopic features may help to differentiate melanosis from malignant pigmented diseases, histopathology remains crucial for the confirming of melanosis of the nipple and areola. Herein, we represent a new case of melanosis of the areola and we describe its clinico‐pathological aspects.
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FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SBCE, SBMB, UL, UM, UPUK
Bone metastases from hepatocellular carcinoma (HCC) are unusual. Optimal treatment strategies are not well defined. The prognosis of this entity is very poor with a survival that does not exceed 1 ...year. We report here a new case of metastatic HCC with longer overall survival of more than 5 years in a 67‐year‐old man.
We report here a new case of metastatic hepatocellular carcinoma with longer overall survival of more than 5 years in a 67‐year‐old man.
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FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SBCE, SBMB, UL, UM, UPUK
Alveolar adenoma is a rare lung benign tumour originating from type II pneumocytes. It presents as a well-defined nodule. In some cases, it is difficult to differentiate from lung cancer. Few cases ...of this tumour have been reported. We describe here a case of alveolar adenoma in a 63-year-old man discovered incidentally on chest X-ray. The lesion was reported as lepidic adenocarcinoma in bronchoscopic biopsy. The patient underwent a thoracoscopic left lower lobectomy. The histopathological and immunohistochemical examinations resulted in a diagnosis of alveolar adenoma. We report this case to describe its morphological and immunohistochemical characteristics and to emphasize its diagnostic difficulties. Keywords: Lung tumour, Alveolar adenoma, Immunohistochemistry, Differential diagnosis
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IZUM, KILJ, NUK, PILJ, PNG, SAZU, UL, UM, UPUK
Primary gastric malignant lymphoma is a rare tumor. The complications associated with lymphoma are perforation, bleeding, or upper gastrointestinal stricture. While it is well known that perforations ...in gastric lymphoma often occurs during chemotherapy, spontaneous perforation is extremely rare in patients who did not receive chemotherapy. This complication requires a surgical treatment.
Primary gastric malignant lymphoma is a rare tumor. The complications associated with this lymphoma are perforation, bleeding, or upper gastrointestinal stricture. While the perforation in gastric lymphoma during chemotherapy is well known, spontaneous perforation is extremely rare in chemotherapy‐naive patients.
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FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SBCE, SBMB, UL, UM, UPUK
Background
Intestinal lipomas are benign, non-epithelial, intestinal tumors with an exceptionally rare localization at the ileum. Lipomas in the small intestine occur mainly in elderly patients and ...seldom occur in childhood. They are frequently asymptomatic, possibly due to their slow growth. These tumors may act as a lead point of intussusception.
Case presentation
We report a rare case of double compounded ileo-ileal intussusception due to a submucosal intestinal lipoma in an 8-year-old female. To our knowledge, this is only the seventh pediatric case to be reported in the medical literature.
Conclusions
Small intestinal submucosal lipoma should be considered in case of intussusception in pediatric patients. Surgical resection seems sufficient in case of symptomatic intestinal lipoma with low morbidity.
Mitochondrial diseases are a clinically heterogeneous group of multisystemic disorders that arise as a result of various mitochondrial dysfunctions. Autosomal recessive aARS deficiencies represent a ...rapidly growing group of severe rare inherited mitochondrial diseases, involving multiple organs, and currently without curative option. They might be related to defects of mitochondrial aminoacyl t-RNA synthetases (mtARS) that are ubiquitous enzymes involved in mitochondrial aminoacylation and the translation process. Here, using NGS analysis of 281 nuclear genes encoding mitochondrial proteins, we identified 4 variants in different mt
ARS
in three patients from unrelated Tunisian families, with clinical features of mitochondrial disorders. Two homozygous variants were found in
KARS
(c.683C>T) and
AARS2
(c.1150-4C>G), respectively in two patients, while two heterozygous variants in
EARS2
(c.486-7C>G) and
DARS2
(c.1456C>T) were concomitantly found in the third patient. Bio-informatics investigations predicted their pathogenicity and deleterious effects on pre-mRNA splicing and on protein stability. Thus, our results suggest that
mtARS
mutations are common in Tunisian patients with mitochondrial diseases.
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EMUNI, FIS, FZAB, GEOZS, GIS, IJS, IMTLJ, KILJ, KISLJ, MFDPS, NLZOH, NUK, OBVAL, OILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
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