Candida albicans (C. albicans) is the most common cause of candidiasis in humans and animals. This study was established to a new experimental infection model for systemic candidiasis using partridge ...and embryonated partridge egg. First, we tested the induction of systemic candidiasis in partridge and embryonated partridge egg. Finally, interaction between virulence factors of C. albicans and Bcl-2 family members was predicted. We observed that embryonic infection causes a decrease in survival time and at later embryonic days (11-12th), embryos showed lesions. Morphometric analysis of the extra-embryonic membrane (EEM) vasculature showed that vascular apoptotic effect of C. albicans was revealed by a significant reduction in capillary area. In immunohistochemistry assay, low expression of Bcl-2 and increased expression of Bax confirmed apoptosis. The gene expression of Bax and Bcl-2 was also altered in fungi-exposed EEM. Ourin silico simulation has shown an accurate interaction between aspartic proteinase, polyamine oxidase, Bcl-2 and BAX. We observed that the disease was associated with adverse consequences, which were similar to human candidiasis. Acquired results support the idea that partridge and embryonated partridge egg can be utilized as appropriate preclinical models to investigate the pathological effects of candidiasis.
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IZUM, KILJ, NUK, PILJ, PNG, SAZU, UL, UM, UPUK
Clostridium perfringens (C. perfringens) is a serious anaerobic enteric pathogen causing necrotic enteritis (NE) in broiler chickens. Following the ban on antibiotics as growth promoters in animal ...feedstuffs, there has been a remarkable rise in occurrence of NE which resulted in considering alternative approaches, particularly vaccination. The objective of this work was to evaluate the recombinant Lactobacillus casei (L. casei) expressing the C-terminal domain of α-toxin from C. perfringens as a potential probiotic-based vaccine candidate to immunize the broiler chickens against NE.
The broiler chickens immunized orally with recombinant vaccine strain were significantly protected against experimental NE challenge, and developed specific serum anti-α antibodies. Additionally, the immunized birds showed higher body weight gains compared with control groups during the challenge experiment.
The current study showed that oral immunization of broiler chickens with a safe probiotic-based vector vaccine expressing α-toxin from C. perfringens could provide protective immunity against NE in birds.
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DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Diabetes Mellitus is associated with disturbances in male reproductive function and fertility. Studies have shown that oxidative stress with the subsequent inflammation and apoptosis cause these ...complications in diabetes. Garlic (G) (Allium sativum L) and Citrullus colocynthis (L.) Schrad (C) both have antidiabetic and antioxidant properties. Recently, we demonstrated their synergistic effects in alleviating reproductive complications when administered concomitantly. However, as even medicinal plants in long term usage may lead to some unwanted side effects of their own, we examined whether with half the original doses of these two medicinal plants we could achieve the desired results.
Thirty-five male Wistar rats were divided into five groups (n = 7/group): Control, Diabetic, Diabetic + G (0.5 ml/100 g BW), Diabetic + C (5 mg/kg BW) and Diabetic + GC (0.5 ml/100 g BW of garlic and 5 mg/kg BW of C. colocynthis) groups. The experimental period was 30 days.
Oxidative stress, advanced glycation end products (AGEs), immunoexpression of caspase-3, and expression of mRNAs for receptor for advanced glycation end products (RAGE), NADPH oxidase-4 (NOX-4) and nuclear factor kappa B increased in testis of diabetic rats. Treatment with garlic and C. colocynthis alone showed some beneficial effects, but in the combination form the effectiveness was more profound.
We conclude that the combination therapy of diabetic rats with lower doses is still as efficient as higher doses; therefore, the way forward for reducing complications in long term consumption.
Over the last years, there has been a remarkable increase in the number of unresponsive patients with anthroponotic cutaneous leishmaniasis (ACL) reported worldwide. The primary objective of this ...study was to explore the role of demographic, clinical and environmental risk related-factors in the development of treatment failure, relapse and chronic cases compared to responsive patients with ACL. Moreover, molecular, histopathological and immunohistochemical (IHC) findings between these forms were explored. This work was undertaken as a prospective and case-control study in southeastern Iran. Culture media and nested PCR were used to identify the causative agent. Univariate multinomial and multiple multinomial logistic regression models and the backward elimination stepwise method were applied to analyze the data. A P<0.05 was defined as significant. Also, for different groups, skin punch biopsies were used to study the histopathological and immunohistochemical (IHC) profile. All samples showed that L. tropica was the only etiological agent in all unresponsive and responsive patients with ACL. Data analysis represented that 8 major risk factors including nationality, age groups, occupation, marital status, history of chronic diseases, duration of the lesion, the lesion on face and presence of domestic animals in the house were significantly associated with the induction of unresponsive forms. The histopathological and immunohistochemical findings were different from one form to another. The present findings clearly demonstrated a positive relation between ACL and distinct demographic, clinical and environmental risk determinants. Knowledge of the main risk factors for ACL infection is crucial in improving clinical and public health strategies and monitor such perplexing factors.
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DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Camels are important sources of milk, meat, wool and leather, and are widely used in transportation in arid and semi-arid areas. But their illnesses, especially parasitic diseases, have not been ...taken into consideration. The
Dipetalonema evansi
microfilariae are in the blood. Adult nematode is only dedicated to camels and disrupts spermatic arteries, lung arteries, right atrium, and testicles. This study was carried out on testicular samples of camels infected with
D. evansi
referred from slaughterhouse. In each of the control and contaminated groups, 5 samples were examined. In this study, in addition to the qualitative description of parasite histopathologic lesions, the spermatogenesis process was evaluated quantitatively including spermatogenesis process, diameter of the seminiferous tubules and Johnsen ranking and compared with the control group. Histopathological examination of infected testis with
D. evansi
showed lumen obstruction of testicular blood vessels by parasites, hypertrophy of blood vessels, degenerative and necrosis changes in the tubules, decreased spermatogenetic activity, increased interstitial space tubules, destruction of the spermatogenic cells. Also, there was a significant difference in the control and contaminated groups in the parameters of spermatogenesis, diameter of the seminiferous tubules and Johnsen score.
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EMUNI, FIS, FZAB, GEOZS, GIS, IJS, IMTLJ, KILJ, KISLJ, MFDPS, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
Due to the anti-inflammatory and antioxidant properties of Scrophularia striata, it is used to heal wounds. Today, hydrogel based on alginate-polyethylene glycol polymer is mainly used due to its ...drug delivery properties and create of suitable conditions for wound healing. In this study, we tried to investigate the effect of hydrogel based on alginate-polyethylene glycol polymer containing Scrophularia striata extract nanoliposomes on wound healing in rat animal models. Field emission scanning electron microscopy and dynamic light scattering were used to examine nanoliposome and hydrogel. 126 Wistar albino rats were randomly divided into 7 groups (18 rats/group). The rats were anesthetized and their dorsum shaved, a burn wound was created with a cylindrical copper at 100° C. Rats' wounds were treated with hydrogel Scrophularia striata extract and on the 5th, 10th, and 15th days, histopathological evaluation, macroscopic features and wound healing were evaluated and analyzed in different groups. The structure of nanoliposomes was uniform and the size was 80-110 nm. Also, the size of the hydrogel was 320 nm with nanometric size and spherical morphology. Histopathological evaluation, wound area and wound contraction confirmed that the treatment group had a significant difference from other groups and the effect was almost similar to that of zinc oxide. This study showed that alginate-polyethylene glycol polymer containing Scrophularia striata extract caused wound contraction, and reduced wound area, and can be used for wound healing.
Objectives
Methionine is known as an essential amino acid in mammals. Consuming excessive amounts of methionine has toxic effects. This study aimed at evaluating the histomorphometric and ...histopathologic changes of ovaries after methionine administration during follicle formation.
Material and methods
A total of 60 newborn female rats born under similar conditions were selected and randomly assigned into three groups including control, recipients of 50 and 200 mg/kg body weight of methionine for 5 days. On day 120, all 60 female rats were euthanized and the whole left ovary of each animal was taken in order to count the number of primordial, primary, secondary, antral, atretic follicles, as well as corpora lutea and also to conduct histopathologic study.
Results
According to the results, the 50 mg/kg methionine did not significantly change the number of primordial follicles compared to the control group but the 200 mg/kg dose significantly decreased the number of primordial follicles. There were no significant differences between the groups in the number of other types of follicles and also in the number of corpora lutea. There was no histopathological lesion in the groups.
Conclusions
It seems that the high dose of methionine could exacerbate apoptosis of the primordial ovarian follicle during the follicle assembly process. However, the remaining were enough to form later stages of follicles after puberty.
A high dose of methionine could exacerbate apoptosis of the primordial ovarian follicle during the follicle assembly process.
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FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SBCE, SBMB, UL, UM, UPUK
The
Cephalopina titillator
is one of the most important causative agents of nasal myiasis in camels. This study aimed to explore the prevalence, histopathological effects, and molecular ...identification of
C. titillator
infestation in camels of Kerman province, South-Eastern Iran, between 2019 and 2021. The larvae were placed in 10% formalin for histopathological evaluation and species identification. Pieces of larval abdominal segments of
C. titillator
were selected for extraction of DNA. Partial mitochondrial CO1 genes were sequenced for final analysis. Out of the 870 camels examined, 339 (38.9%) were infested with larval stages of
C. titillator
. There was a significant difference between age and infection rate (
P
= 0.001), while no association between males and females (
P
= 0.074) was found. The infection rate was significantly higher in the winter (
P
< 0.001) than in the other seasons. In this study, different lesions depending on duration, locations, and the depth of larval adhesion notably degeneration changes, necrosis, and ulceration were observed. Also, in chronic cases, granulation tissue reactions were organized.
Cephalopina titillator
was confirmed by PCR sequencing analysis using mitochondrial CO1 region. A 582 bp nucleotide sequence was deposited in GenBank under the MW136151 accession number. Phylogenetic analysis of CO1 produced a single uniform sister clade to MZ209004 and MW167083 records from China and Iraq, respectively. The high prevalence of
C. titillator
in camels in this region and other areas of Iran declares that the country is in an endemic status and displays the existence of the potential risk for camels.
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EMUNI, FIS, FZAB, GEOZS, GIS, IJS, IMTLJ, KILJ, KISLJ, MFDPS, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
Onchocerca fasciata is a prevalent filarial species in camelids of Asia and Africa forming nodules in the skin of dromedary and Bactrian camels. In spite of recent advances in the biology and ...epidemiology of this nematode species, a relatively scant number of studies have focussed on the morphology of this parasite. The main objective of this study was to describe morphological characteristics of adults, microfilariae and eggs of O. fasciata by scanning electron microscopy (SEM), staining and histology.
From April 2016 to March 2017 dromedary camels (n = 456) were inspected for infection with O. fasciata in a slaughterhouse in Kerman (south of Iran). Adult worms in nodules were isolated by digestion of nodules in collagenase and used for SEM. Skin nodules were also fixed, sectioned and stained with hematoxylin and eosin for histopathology. Skin microfilariae that were isolated from tissues surrounding the nodules were confirmed as O. fasciata by sequencing of the cytochrome c oxidase subunit 1 (cox1) and 12S rRNA genes and used for SEM and Giemsa staining.
Single or multiple O. fasciata nodules (1.2-2.2 cm in diameter and 507-845 mg in weight) were found in 30.3% of the examined camels. SEM analysis helped identify 18 papillae in the caudal region of the male. Discontinuous longitudinal cuticular crests were observed in the posterior region of the male. In female nematodes, the ridges had a rounded shape with a height/width ratio of 7/16 in longitudinal sections. Unsheathed skin microfilariae with a rounded anterior extremity measured 210.7 × 2.5 μm on average. Developed eggs containing microfilariae measured 35.9 × 31.0 μm and their smooth shell surface had characteristic tongue-like appendages. In addition to inflammatory reactions surrounding the parasites, accumulation of intracellular ceroid pigment, golden-yellow to brown in colour, was observed within macrophages upon histopathological examination.
We found longitudinal crests on the surface of the posterior region of the male nematode. Measurements of the main morphological features of microfilariae and eggs, and the shape index of ridges (height/width) in female nematodes are described for the first time.
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IZUM, KILJ, NUK, PILJ, PNG, SAZU, UL, UM, UPUK
Background: Oxymetholone, an anabolic-androgenic steroid, has been used to treat some diseases. The abuse of this compound, especially for muscle growth, has severe oxidative side effects on the ...liver and kidneys. Oxidative stress and free radicals are responsible for the development of various diseases. Phytochemicals are sources of polyphenols, flavonoids, saponins, etc. and may act as scavengers of free radicals. Saffron (Crocus sativus) has considerable antioxidant properties, which may be useful in reversing or preventing these negative effects. Methods: To evaluate the effects of saffron on the liver and kidneys degenerative changes induced by oxymetholone, 24 male Wistar rats were used. The rats were randomly divided into four groups of six rats each as: a) sham (normal saline, 40 mg/kg/day), b) saffron control (40 mg/kg/day), c) oxymetholone (50 mg/rat), and d) treatment group saffron (40 mg/kg/day)+oxymetholone (50 mg/rat). The course of examination was 30 days. Results: After one month, the sham and saffron control groups had normal histological findings. The treatment group with saffron showed a significant reduction in the hepatic and renal degenerative changes induced by oxymetholone compared to those observed in group. Conclusion: Based on the histopathological results, the use of saffron produced protective effects against the degenerative changes in rats’ liver and kidneys induced by oxymetholone.
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