Ovaj rad se bavi otkrivanjem znanja primjenom postupaka strojnog učenja iz baze podataka web aplikacije iz društvenog područja. Izvršena je dubinska analiza podataka pomoću četiri različite metode na ...skupu podataka vezanih uz prijave i pohađanje poslovnih događaja organiziranih od strane jedne tvrtke u razdoblju od tri godine. Primarni cilj rada je otkrivanje znanja iz postojećeg skupa podataka. Pokazano je kako su se deskriptivnom dubinskom analizom otkrila nova znanja. Za analizu su korištene metode stabla odlučivanja, slučajne šume, indukcija pravila te metoda potpornih vektora. Proučene su razlike između navedenih metodologija, te prednosti i mane svake od njih s naglaskom na točnost i utrošak vremena. Osim toga, u radu je opisan i proces pripreme podataka koji je vremenski najzahtjevniji, te sami brojčani rezultati dobiveni prediktivnom analizom.
Progresivne web aplikacije su trend u svijetu web programiranja jer pružaju moćni skup funkcionalnosti spajajući najbolje strane web i mobilnih aplikacija. Kod planiranja izrade, česta je dilema da ...li se odlučiti za web ili mobilnu aplikaciju, a najčešće je potrebno razviti obje verzije aplikacije kako bi došli do što većeg broja korisnika. Progresivne web aplikacije koriste moderne mogućnosti web preglednika kako bi postigle korisničko iskustvo vrlo slično mobilnim aplikacijama a u okviru klasičnih web aplikacija, time kombinirajući najbolje od oba svijeta. U ovome radu su analizirane funkcionalnosti progresivnih web aplikacija, te je obrazloženo zašto pružaju odlično korisničko iskustvo i zašto su sve češća pojava na tržištu. Osim prednosti su proučene i mane, te su na primjeru gotove progresivne web aplikacije testirane preformanse i značajke takve aplikacije
U ovom radu prikazan je postupak razvoja aplikacije za vođenje online knjižnice te su opisane i komentirane korištene tehnologije. Cilj je stvoriti intuitivnu i jednostavnu aplikaciju za ...katalogizaciju i pretraživanje knjižničke građe, te omogućiti korisnicima da rezerviraju knjige i prate svoje posudbe. Opisani su kriteriji odabira tehnologija kao i glavne funkcionalonosti aplikacije. Aplikacija koristi Firebase kao bazu podataka za pohranu podataka o knjigama i novostima, ali i kao sustav za autentifikaciju korisnika. Za izradu prednjeg dijela aplikacije korišten je radni okvir Vue.js. Rad obuhvaća proces izrade te prikazuje gotov proizvod, uključujući funkcionalnosti aplikacije, vrste korisnika aplikacije te korištenje digitalne članske iskaznice za jednostavniju identifikaciju korisnika.
A series of novel amidino 2-substituted benzimidazoles linked to 1,4-disubstituted 1,2,3-triazoles were synthesized by implementation of microwave and ultrasound irradiation in click reaction and ...subsequent condensation of thus obtained 4-(1,2,3-triazol-1-yl)benzaldehyde with o-phenylenediamines. In vitro antiproliferative screening of compounds performed on human cancer cell lines revealed that p-chlorophenyl-substituted 1,2,3-triazolyl N-isopropylamidine 10c and benzyl-substituted 1,2,3-triazolyl imidazoline 11f benzimidazoles had selective and potent cytostatic activities in the low nM range against non-small cell lung cancer cell line A549, which could be attributed to induction of apoptosis and primary necrosis. Additional Western blot analyses showed different mechanisms of cytostatic activity between compounds 10c and 11f that could be associated with the nature of aromatic substituent at 1-(1,2,3-triazolyl) and amidino moiety at C-5 position of benzimidazole ring. Specifically, compound 11f abrogated the activity of several protein kinases including TGM2, CDK9, SK1 and p38 MAPK, whereas compound 10c did not have profound effect on the activities of CDK9 and TGM2, but instead showed moderate downregulation of SK1 activity concomitant with a significant reduction in p38 MAPK. Further in silico structural analysis demonstrated that compound 11f bound slightly better to the ATP binding site of p38 MAPK compared to 10c, which correlated well with observed stronger decrement in the expression level of phospho-p38 MAPK elicited by 11f in comparison with 10c.
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•Novel amidino 2-substituted benzimidazoles linked to 1,2,3-triazoles were prepared.•N-isopropylamidine 10c and imidazoline 11f benzimidazoles exhibited selective and potent effects against A549 cells in the low nM range.•Molecular docking analyses of 10c and 11f in the ATP binding site of p38 MAPK were carried out.•Inhibitory activity in A549 cells was associated with induction of apoptosis and primary necrosis.•Cytostatic and pro-apoptotic effects of 10c and 11f could be ascribed to multi-target inhibition.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPUK, ZRSKP
Novel halogenated purines and pseudopurines with diverse aryl-substituted 1,2,3-triazoles were prepared. While p-(trifluoromethyl)-substituted 1,2,3-triazole in N-9 alkylated purine and 3-deazapurine ...was critical for strong albeit unselective activity on pancreatic adenocarcinoma cells CFPAC-1,1-(p-fluorophenyl)-1,2,3-triazole derivative of 7-deazapurine showed selective cytostatic effect on metastatic colon cancer cells SW620. Importantly, 1-(p-chlorophenyl)-1,2,3-triazole-tagged benzimidazole displayed the most pronounced and highly selective inhibitory effect in nM range on non-small cell lung cancer A549. This compound revealed to target molecular processes at the extracellular side and inside the plasma membrane regulated by GPLD1 and growth factor receptors PDGFR and IGF-1R leading to the inhibition of cell proliferation and induction of apoptosis mediated by p38 MAP kinase and NF-κB, respectively. Further optimisation of this compound as to reduce its toxicity in normal cells may lead to the development of novel agent effective against lung cancer.
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DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, UILJ, UKNU, UL, UM, UPUK
Acute ischemic stroke (AIS) is the world’s second leading cause of mortality. An established method for treating stroke patients in acute settings is endovascular therapy (EVT). However, the ...correlation of the successful endovascular treatment of AIS with the presence of communicating arteries in the circle of Willis needs to be proven. Our study examined clinical and radiological data of 158 consecutive patients treated with mechanical thrombectomy (MT) at our comprehensive stroke center. We analyzed their CT angiograms and digital subtraction angiography (DSA) to assess anatomical variants of Willis’ circle and formed two groups—collateral-negative and collateral-positive group. The first group included patients with aplasia of both anterior (ACoA) and posterior communicating Artery (PCoA). The second group included patients that have at least one communicating artery (either anterior or posterior). We evaluated their reperfusion outcomes and functional recovery three months later. Our results showed that patients with communicating arteries had smaller areas of infarction on post-interventional CT and higher rates of functional recovery (Modified Rankin Score). The ACoA had a higher impact on early and late outcomes, confirmed by lower control CT scores and more favorable functional recovery. Therefore, anatomic variants of Willis’ circle should be considered as a significant prognostic factor in AIS.
AIMTo assess the differences in clinical and laboratory parameters of non-hospitalized patients with deep venous thrombosis (DVT) according to the SARS-CoV-2 status. METHODSWe retrospectively ...reviewed demographic, clinical, laboratory, and ultrasound data of adult patients admitted to the Emergency Department of University Hospital Split between March 2020 and January 2021. Patients were classified into three groups: recent COVID-19 (<1 month), non-recent COVID-19 (1 to 12 months), and non-COVID-19. RESULTSFifty (47.2%) of 106 patients had a history of SARS-CoV-2 infection (23 patients in the recent COVID-19 and 27 in non-recent COVID-19 group). The three groups did not significantly differ in demographic and clinical parameters, including the location of deep venous thrombosis. The recent COVID-19 group had significantly higher neutrophils and CRP levels, and significantly lower prothrombin than the other two groups. CONCLUSIONOur results confirm the role of elevated inflammatory and coagulation response in DVT development in the first month after the infection, but not in non-recent COVID-19 or non-COVID-19 patients.
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DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Acute ischemic stroke (AIS) is one of the leading causes of morbidity worldwide, thus, early recognition is essential to accelerate treatment. The only definite way to diagnose AIS is radiological ...imaging, which is limited to hospitals. However, two serum neuromarkers, glial fibrillary acidic protein (GFAP) and ubiquitin C-terminal hydrolase-L1 (UCH-L1), have been proven as indicators of brain trauma and AIS. We aimed to investigate the potential utility of these markers in distinguishing between large vessel occlusion (LVO) and small vessel occlusion (SVO), considering differences in treatment. Sixty-nine AIS patients were included in our study and divided into LVO and SVO groups based on radiological imaging. Control group consisted of 22 participants without history of neurological disorders. Results showed differences in serum levels of both GFAP and UHC-L1 between all groups; control vs. SVO vs. LVO (GFAP: 30.19 pg/mL vs. 58.6 pg/mL vs. 321.3 pg/mL; UCH-L1: 117.7 pg/mL vs. 251.8 pg/mL vs. 573.1 pg/mL;
< 0.0001), with LVO having the highest values. Other prognostic factors of stroke severity were analyzed and did not correlate with serum biomarkers. In conclusion, a combination of GFAP and UCH-L1 could potentially be a valuable diagnostic tool for differentiating LVO and SVO in AIS patients.
Couples with infertility issues have been assisted by
fertilization reproduction technologies with high success rates of 50-80%. However, complications associated with ovarian stimulation remain, ...such as ovarian hyperstimulation. Oocyte quality is a significant factor impacting the outcome of
fertilization procedures, but other processes are also critical for fertilization success. Increasing evidence points to aberrant inflammation as one of these critical processes reflected in molecular changes, including glycosylation of proteins. Here we report results from a MALDI-TOF-MS-based glycomic profiling of the total IgG and total proteome N-glycomes isolated from the follicular fluid obtained from patients undergoing fertilization through either (1) assisted reproduction by modified natural cycle or (2) controlled ovarian stimulation (GnRH antagonist, GnRH Ant) protocols. Significant inflammatory-related differences between analyzed N-glycomes were observed from samples and correlated with the ovarian stimulation protocol used in patients.
Circadian clock regulation in mammals is controlled by feedback loops of a set of circadian genes. One of these circadian genes, NPAS2, encodes for a member of the bHLH-PAS class of transcription ...factors and is expressed in the forebrain and in some peripheral organs such as liver and skin. Other biological processes are also regulated by circadian genes. For example, NPAS2 is involved in cell proliferation, DNA damage repair and malignant transformation. Aberrant expression of clock genes has been previously observed in melanoma which led to our effort to sequence the NPAS2 promoter region in this cancer type. The NPAS2 putative promoter and 5′ untranslated region of ninety-three melanoma patients and ninety-six control subjects were sequenced and several variants were identified. Among these is a novel microsatellite comprising a GGC repeat with different alleles ranging from 7 to 13 repeats located in the 5′ untranslated exon. Homozygosity of an allele with nine repeats (9/9) was more prevalent in melanoma than in control subjects (22.6% and 13.5%, respectively, P: 0.0206) suggesting that some NPAS2 variants might contribute to melanoma susceptibility.
Impact statement
This report describes a variable microsatellite repeat sequence located in the 5′ untranslated exon of NSPAS2, a gene encoding a clock transcription factor. Significantly, this study is the first to show that a variant copy number GGC repeat sequence in the NPAS2 clock gene associates with melanoma risk and which may be useful in the assessment of melanoma predisposition.
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