•Structural characterization of two polysaccharides from Dendrobium officinale were described.•DOP performed the hypoglycemic activity via stimulating GLP-1 secretion in STZ-induced diabetic rats and ...STC-1 cells.•Ca2+/CaM/CaMKII and MAPK pathways might involve in the intracellular DOP-induced GLP-1 secretion.•The repeated unit of backbone of DOP-1 and DOP-2 might be the effective unit of DOP-induced GLP-1 secretion.
Two polysaccharides, named DOP-1 and DOP-2, with molecular weights of 6.8 kDa and 14.3 kDa, respectively, were isolated and purified from the stems of Dendrobium officinale. Monosaccharide composition, Fourier-transform infrared spectroscopy, methylation, and nuclear magnetic resonance analyses indicated that DOP-1 and DOP-2 may have a backbone consisted of →4)-β-d-Glcp-(1→, →4)-β-d-Manp-(1→, →4)-2-O-acetyl-β-d-Manp-(1→ and →4)-3-O-acetyl-β-d-Manp-(1→. In vivo assays showed that D. officinale polysaccharides (DOPs) exerted significant hypoglycemic effects accompanying increased serum insulin and glucagon-like peptide-1 (GLP-1) levels in streptozotocin-induced diabetic rats. Further in vitro experiments showed that DOP-induced GLP-1 secretion was inhibited by an intracellular calcium chelator, a Ca2+/calmodulin-dependent protein kinase (CaMK) II inhibitor, a specific calcium-sensing receptor antagonist, and a p38-mitogen-activated protein kinases (MAPK) inhibitor. These results indicated that DOPs may decrease fasting blood sugar levels by stimulating GLP-1 secretion and that intracellular DOP-induced GLP-1 secretion involved the Ca2+/calmodulin/CaMKII and MAPK pathways.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
A novel polysaccharide (MSP-1) was isolated from the fruiting body of Morchella sextelata and purified using DEAE-52 and Sephadex G-75. The molecular weight of MSP-1 was 1.17 × 104 Da, as detected by ...HPLC analysis. The monosaccharide composition of MSP-1 was mannose and glucose at a ratio of 1.00: 1.25. Methylation and NMR results revealed that the backbone of MSP-1 was composed of →4)-β-D-Manp-(1→, →4)-β-D-Glcp-(1→, →4)-α-D-Glcp-(1→, and →4, 6)-α-D-Glcp-(1→. SEM images of MSP-1 presented a dense network structure with porous characterizations. The immunomodulatory activities of MSP-1 were evaluated using RAW264.7 cells, and the results showed that MSP-1 promoted proliferative and phagocytic activity and increased the production of NO, TNF-α and IL-6. These results indicated that MSP-1 exhibited significant immunomodulatory activities.
•A novel heteropolysaccharide (MSP-1) was purified from the fruiting body of Morchella sextelata.•The complete structure of M. sextelata polysaccharide was exhibited for the first time in the present study.•The 3D structure of MSP-1 presented a dense network structure with porous characterizations.•MSP-1 exhibited strong immunomodulatory activities by promoting proliferative and phagocytic activity and obviously increasing the production of NO, TNF-α and IL-6 on RAW264.7 cells.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
The traditional Chinese medicine (TCM) “Shihu” has a long history of medicinal use in China from some species of Dendrobium. D. officinale is a major source of “Shihu” and is widely cultivated in ...south of China and listed separately as “Tiepi Shihu” by the Chinese Pharmacopoeia in now time. Traditionally, D. officinale has been widely used in daily health care and the treatment of diabetes and gastrointestinal diseases.
In order to better develop and utilize D. officinale, we conducted this systematic review of previous studies, showed clear structure of all isolates from D. officinale together with pharmacological progress, hoping to provide references for further research and utilization. In addition, specific display of the chemical components and the research progress of related activities can help to better understand the traditional records and modern pharmaceutical applications of the plant medicine.
Information on phytochemistry and pharmacological studies of D. officinale was collected from various scientific databases including Web of Science, SciFinder, ACS, Springer, Scopus, PubMed, ScienceDirect, Google Scholar and CNKI.
More than 180 compounds isolated from D. officinale, including bibenzyls, phenols, phenylpropanoids, lignans, flavonoids and polysaccharides are listed in this review. Furthermore, modern pharmacological researches such as hypoglycemia, immune regulation, antioxidant, cardiovascular regulation and gastrointestinal protection are summarized.
Based on the summary of the research work of D. officinale, we systematically show the chemical composition of the plant, and concluded the relationship of those composition with plant habitat together with the relationship between the structure of chemical components and pharmacological activity. Moreover, we suggest that some of small molecule compounds could also be quality control of D. officinale besides polysaccharides.
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•D. officinale is widely used in Asia as a precious folk herbal medicine or health food.•Traditional application of D. officinale and modern diseases, especially diabetes, were discussed.•Active ingredients and their action mechanism of D. officinale were systematically summarized.•The relationship between habitat and chemical composition of D. officinale was discussed.•This review intends to provide a comprehensive understanding and appraisal of D. officinale.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Extending the two-dimensional topology of wireless networks to the three-dimensional (3D) structure has recently been recognized as a revolutionary breakthrough for the next generation of wireless ...communication networks. As revealed in the literature, dispatching the unmanned aerial vehicle (UAV) assisted base-station (UBS) can fill in the coverage holes as the low energy-efficiency (EE) BSs on the ground are switched off. De facto, properly deploying the UBSs can further enlarge the flexibility of the user handover and exchange procedures in the sleep strategy. Thus, in this paper, we propose a low-complexity UBS-assisted sleep strategy for the small-cell network (SCN). Verified by the simulation results, it is found that deploying a proper number of capable UBSs can remarkably raise the EE for the 3D SCNs.
Cell-cell communication via ligand-receptor signaling is a fundamental feature of complex organs. Despite this, the global landscape of intercellular signaling in mammalian liver has not been ...elucidated. Here we perform single-cell RNA sequencing on non-parenchymal cells isolated from healthy and NASH mouse livers. Secretome gene analysis revealed a highly connected network of intrahepatic signaling and disruption of vascular signaling in NASH. We uncovered the emergence of NASH-associated macrophages (NAMs), which are marked by high expression of triggering receptors expressed on myeloid cells 2 (Trem2), as a feature of mouse and human NASH that is linked to disease severity and highly responsive to pharmacological and dietary interventions. Finally, hepatic stellate cells (HSCs) serve as a hub of intrahepatic signaling via HSC-derived stellakines and their responsiveness to vasoactive hormones. These results provide unprecedented insights into the landscape of intercellular crosstalk and reprogramming of liver cells in health and disease.
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•Heterogeneity and plasticity of non-parenchymal cells in healthy and NASH liver•Landscape of intrahepatic ligand-receptor signaling at single-cell resolution•Emergence of Trem2+ NASH-associated macrophages (NAMs) in mouse and human NASH•Stellakine secretion and contractile response to vasoactive hormones by HSCs
This work illustrates the heterogeneity of liver non-parenchymal cells (NPCs) and their reprogramming during NASH pathogenesis. Using single-cell RNA-sequencing analysis, the authors mapped the landscape of the intrahepatic ligand-receptor signaling network and revealed two fundamental aspects of HSC biology: stellakine secretion and contractile response to vasoactive hormones. Hepatic vascular dysfunction and emergence of Trem2+ NASH-associated macrophages (NAMs) are two conserved features of mouse and human NASH.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
The performance of gas sensors is greatly influenced under humidity environment due to the dynamic correlation between water and oxygen molecules. In this work, we utilize the charge trapping ...characteristic to study surface gas adsorption and demonstrate the competitive adsorption behaviors between oxygen and water vapor from the perspective of electrical dipole by observing the evolution of second harmonic generation (SHG). By analyzing the trapping time constant of time-dependent SHG spectrum, we propose a dynamic model of the way water molecules weaken oxygen adsorption and develop water adsorption layer. Results show that the electric field created by charge trapping would further align the water molecules and enhance the SHG intensity. Here, a viewpoint of analyzing the net surface dipole change due to the adsorption–desorption on SiO2 surface to realize the energy preferable state is presented and provides a pathway for solving the water–sensor interaction problems in gas sensor.
•H2O molecules influence the performance of gas sensor and complicate the analysis.•Internal photoemission induces charge traps on SiO2 surface via laser irradiation.•Competition between O2 and H2O molecules is observed by second harmonic generation.•Evolution of electric dipoles and charge trapping of adsorption–desorption process.•H2O has more competitive advantage than O2 adsorption on dehydroxylated SiO2.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Previous research has suggested that problematic Internet use (PIU) is associated with impulse control disorder. Although researchers have suggested that impulsivity is a risk factor for PIU, the ...literature lacks longitudinal evidence on the relationship between impulsivity and PIU. We aimed to use a cross‐lagged analytic framework to identify temporal order effects and hypothesised that impulsivity was the precedent factor for PIU. In a panel sample of college students (N = 367), trait impulsivity and PIU were measured in the spring of freshman year and in their junior year. The measures included a self‐developed PIU Scale and the revised Impulsiveness Scale based on Barratt's concept. We found that “non‐planning impulsivity” was not associated with PIU. The “motor impulsivity” subfactor was thus adopted in the cross‐lagged model. The results suggest that motor impulsivity and PIU were stable across time. Motor impulsivity at Time 1 positively predicted PIU at Time 2, but PIU at Time 1 did not predict motor impulsivity at Time 2. A further investigation using gender as a moderator found a gender difference in the temporal relationship. Because motor impulsivity is a risk factor for PIU, potential prevention strategies based on this result are suggested.
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DOBA, FSPLJ, FZAB, GIS, IJS, IZUM, KILJ, NLZOH, NUK, OILJ, PILJ, PNG, SAZU, SBCE, SBMB, SIK, UILJ, UKNU, UL, UM, UPUK
The first total synthesis of gastrodinol, an unprecedented poly-p-cresol-substituted natural product with a rearranged and reconstructed C ring moiety, is reported. Our synthesis features a ...convergent fragment approach. The Sonogashira coupling reaction forges the two segments together to furnish the conjugated ene–yne. Photocatalytic 6π electrocyclization followed by spontaneous aromatization is used to construct the tetrasubstituted B ring at the late stage. Further study shows that gastrodinol exhibits significant cytotoxic activity against five human cancer cell lines in vitro (IC50 2.5–3.8 μM).
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IJS, KILJ, NUK, PNG, UL, UM
This study assessed the performance of visceral adiposity index and body shape index in predicting diabetes mellitus (DM) risk and compared their predictive ability to that of body mass index and ...waist circumference. Among 8249 consecutive subjects who attended the Nationwide Health Check Up System for Senior Citizens (greater than or equai to 65 years) between 2008 and 2018, we examined the associations of several adiposity indices with DM risk and explored gender differences. Among all adiposity indicators, Chinese visceral adiposity index (CVAI) demonstrated the highest discriminatory ability for diabetes mellitus with area under receiver operating characteristic curves (AUC) of 0.65, 0.68, and 0.66 for men, women, and all participants, respectively, and optimal cut-offs set as 126.09 in men and 117.77 in women. Compared with body shape index (ABSI), both CVAI and VAI were strongly associated with baseline DM (adjusted OR: 4.85, 95% CI: 4.05-5.82 and 4.22, 95% CI: 3.53-5.05 for 4th vs 1st quartile groups by CVAI and VAI, P < 0.001), which was more pronounced in older adult women (P.sub.interaction < 0.05). Over a median of 5.25 years (IQR: 3.07-6.44 years) follow-up, Cox regression models showed higher predictive ability of CVAI and VAI compared to ABSI. Further, both CVAI and VAI independently predicted new-onset DM (adjusted HR: 1.29, 95% CI: 1.22-1.37 and 1.16, 95% CI: 1.11-1.21 by CVAI and VAI) and composite endpoint of new DM and death among those without baseline DM. Our population-based data demonstrated that Chinese visceral adiposity index may serve as a superior clinical indicator of diabetes when compared with conventional anthropometric indices among older adult Chinese, especially in women.
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DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK, VSZLJ
Non-destructive analysis of dopant concentration is crucial in the process control of advanced semiconductor fabrication. Here, the dopant concentration of doped Si ultrathin film (DSUTF) which is ...around 10 nm is evaluated non-destructively by second harmonic generation (SHG). The technique is based on analyzing the evolution of the internal photoemission induced charge trapping and the concomitant electric field induced SHG. We further demonstrate a strategy to estimate the dopant concentration by considering the Fermi-Dirac distribution and the tunneling probability, without involving the crystallinity of DSUTF. The dopant concentration between 1017 and 1020 (atom/cm3) is unambiguously evaluated by this method. The unprecedented approach of using non-destructive method to reveal dopant concentration of DSUTF via time-dependent SHG constitutes an important step towards in-line monitoring and optimizing the fabrication conditions.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
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