Long noncoding RNAs (lncRNAs) have gained widespread attention in recent years as a potentially new and crucial layer of biological regulation. lncRNAs of all kinds have been implicated in a range of ...developmental processes and diseases, but knowledge of the mechanisms by which they act is still surprisingly limited, and claims that almost the entirety of the mammalian genome is transcribed into functional noncoding transcripts remain controversial. At the same time, a small number of well-studied lncRNAs have given us important clues about the biology of these molecules, and a few key functional and mechanistic themes have begun to emerge, although the robustness of these models and classification schemes remains to be seen. Here, we review the current state of knowledge of the lncRNA field, discussing what is known about the genomic contexts, biological functions, and mechanisms of action of lncRNAs. We also reflect on how the recent interest in lncRNAs is deeply rooted in biology's longstanding concern with the evolution and function of genomes.
Regulated biophysical cues, such as nanotopography, have been shown to be integral for tissue regeneration and embryogenesis in the stem cell niche. Tissue homeostasis involves the interaction of ...multipotent cells with nanoscaled topographical features in their ECM to regulate aspects of cell behavior. Synthetic nanostructures can drive specific cell differentiation, but the sensing mechanisms for nanocues remain poorly understood. Here, we report that nanotopography-induced human mesenchymal stem cell (hMSC) differentiation through cell mechanotransduction is modulated by the integrin-activated focal adhesion kinase (FAK). On nanogratings with 250 nm line width on polydimethylsiloxane, hMSCs developed aligned stress fibers and showed an upregulation of neurogenic and myogenic differentiation markers. The observed cellular focal adhesions within these cells were also significantly smaller and more elongated on the nanogratings compared to microgratings or unpatterned control. In addition, our mechanistic study confirmed that this regulation was dependent upon actomyosin contractility, suggesting a direct force-dependent mechanism. The topography-induced differentiation was observed on different ECM compositions but the response was not indicative of a direct ECM-induced hMSC differentiation pathway. FAK phosphorylation was required for topography-induced hMSC differentiation while FAK overexpression overruled the topographical cues in determining cell lineage bias. The results indicated that FAK activity had a direct impact on topography-induced gene expression, and that this effect of FAK was independent of cell shape. These findings suggest that hMSC sense and transduce nanotopographical signals through focal adhesions and actomyosin cytoskeleton contractility to induce differential gene expression.
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IJS, KILJ, NUK, PNG, UL, UM
Stacking atomic monolayers of semiconducting transition metal dichalcogenides (TMDs) has emerged as an effective way to engineer their properties. In principle, the staggered band alignment of TMD ...heterostructures should result in the formation of interlayer excitons with long lifetimes and robust valley polarization. However, these features have been observed simultaneously only in MoSe2/WSe2 heterostructures. Here we report on the observation of long-lived interlayer exciton emission in a MoS2/MoSe2/MoS2 trilayer van der Waals heterostructure. The interlayer nature of the observed transition is confirmed by photoluminescence spectroscopy, as well as by analyzing the temporal, excitation power, and temperature dependence of the interlayer emission peak. The observed complex photoluminescence dynamics suggests the presence of quasi-degenerate momentum-direct and momentum-indirect bandgaps. We show that circularly polarized optical pumping results in long-lived valley polarization of interlayer exciton. Intriguingly, the interlayer exciton photoluminescence has helicity opposite to the excitation. Our results show that through a careful choice of the TMDs forming the van der Waals heterostructure it is possible to control the circular polarization of the interlayer exciton emission.
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IJS, KILJ, NUK, PNG, UL, UM
Philosophers and behavioral scientists refer to wisdom as unbiased reasoning that guides one toward a balance of interests and promotes a good life. However, major instruments developed to test ...wisdom appear biased, and it is unclear whether they capture balance-related tendencies. We examined whether shifting from global, de-contextualized reports to state-level reports about concrete situations provides a less biased method to assess wise reasoning (e.g., intellectual humility, recognition of uncertainty and change, consideration of the broader context at hand and perspectives of others, integration of these perspectives or compromise), which may be aligned with the notion of balancing interests. Results of a large-scale psychometric investigation (N = 4,463) revealed that the novel Situated WIse Reasoning Scale (SWIS) is reliable and appears independent of psychological biases (attribution bias, bias blind spot, self-deception, and impression management), whereas global wisdom reports are subject to such biases. Moreover, SWIS scores were positively related to indices of living well (e.g., adaptive emotion regulation, mindfulness), and balancing of cooperative and self-protective interests, goals (influence-vs.-adjustment), and causal inferences about conflict (attribution to the self-vs.-other party). In contrast, global wisdom reports were unrelated or negatively related to balance-related measures. Notably, people showed modest within-person consistency in wise reasoning across situations or over time, suggesting that a single-shot measurement may be insufficient for whole understanding of trait-level wisdom. We discuss theoretical and practical implications for research on wisdom, judgment and decision making, well-being, and prosociality.
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CEKLJ, FFLJ, NUK, ODKLJ, PEFLJ, UPUK
We hypothesized that a wisdom-based reasoning process comprised of epistemic humility, accounting for context, and integrating different perspectives and interests, would be helpful in overcoming ...intergroup bias and attitude polarization in societal conflicts. Here we test the hypothesis using both the Situated Wise Reasoning Scale and experimental induction. In each study, we recruited participants who self-identified as members of a group implicated in an ongoing intergroup situation. In five correlational studies (Studies 1-5) we examined the relations between measured wise reasoning and intergroup positivity and attitude polarization. In two experiments, we tested the effects of a brief online wise-reasoning thought exercise on intergroup positivity and polarization (Studies 6-7), and charitable behaviors to an outgroup (Study 6). We found that wise reasoning relates to more positivity toward outgroups and less attitude polarization across different groups and conflicts. The results have implications for theory and may also have implications for future research on interventions to improve intergroup relations.
Historically, the study of multiple goals has focused on the dynamics between two goals as the prototypical example of multiple goals. This focus on dyadic relations means that many issues central to ...the psychology of more than two goals are still unexplored. We argue that a deeper understanding of multiple-goal issues involves moving beyond two goals. Doing so not only reveals new insights about goal relations (e.g., how one dyadic relation affects another) but also introduces goal structure (how goals and goal relations are positioned relative to each other) as a variable in its own right worthy of study. In our review, we discuss current knowledge gaps, review methodologies both in terms of existing techniques and novel techniques we propose, and highlight new directions from moving beyond two goals—what new questions emerge and what dynamics, including intersectional issues (e.g., between goal properties and goal structure), become possible to explore.
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NUK, OILJ, SAZU, UKNU, UL, UM, UPUK
Ecological dynamics of emerging bat virus spillover Plowright, Raina K.; Eby, Peggy; Hudson, Peter J. ...
Proceedings - Royal Society. Biological sciences/Proceedings - Royal Society. Biological Sciences,
01/2015, Volume:
282, Issue:
1798
Journal Article
Peer reviewed
Open access
Viruses that originate in bats may be the most notorious emerging zoonoses that spill over from wildlife into domestic animals and humans. Understanding how these infections filter through ecological ...systems to cause disease in humans is of profound importance to public health. Transmission of viruses from bats to humans requires a hierarchy of enabling conditions that connect the distribution of reservoir hosts, viral infection within these hosts, and exposure and susceptibility of recipient hosts. For many emerging bat viruses, spillover also requires viral shedding from bats, and survival of the virus in the environment. Focusing on Hendra virus, but also addressing Nipah virus, Ebola virus, Marburg virus and coronaviruses, we delineate this cross-species spillover dynamic from the within-host processes that drive virus excretion to land-use changes that increase interaction among species. We describe how land-use changes may affect co-occurrence and contact between bats and recipient hosts. Two hypotheses may explain temporal and spatial pulses of virus shedding in bat populations: episodic shedding from persistently infected bats or transient epidemics that occur as virus is transmitted among bat populations. Management of livestock also may affect the probability of exposure and disease. Interventions to decrease the probability of virus spillover can be implemented at multiple levels from targeting the reservoir host to managing recipient host exposure and susceptibility.
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BFBNIB, NMLJ, NUK, PNG, SAZU, UL, UM, UPUK
How coherent quasiparticles emerge by doping quantum antiferromagnets is a key question in correlated electron systems, whose resolution is needed to elucidate the phase diagram of copper oxides. ...Recent resonant inelastic X-ray scattering (RIXS) experiments in hole-doped cuprates have purported to measure high-energy collective spin excitations that persist well into the overdoped regime and bear a striking resemblance to those found in the parent compound, challenging the perception that spin excitations should weaken with doping and have a diminishing effect on superconductivity. Here we show that RIXS at the Cu L3-edge indeed provides access to the spin dynamical structure factor once one considers the full influence of light polarization. Further we demonstrate that high-energy spin excitations do not correlate with the doping dependence of Tc, while low-energy excitations depend sensitively on doping and show ferromagnetic correlations. This suggests that high-energy spin excitations are marginal to pairing in cuprate superconductors.
The minimum energy required to transmit one bit of information through a network characterizes the most economical way to communicate in a network. In this paper, we show that, under a layered model ...of wireless networks, the minimum energy-per-bit for multicasting in a mobile ad hoc network can be found by a linear program; the minimum energy-per-bit can be attained by performing network coding. Compared with conventional routing solutions, network coding not only allows a potentially lower energy-per-bit to be achieved, but also enables the optimal solution to be found in polynomial time, in sharp contrast with the NP-hardness of constructing the minimum-energy multicast tree as the optimal routing solution. We further show that the minimum energy multicast formulation is equivalent to a cost minimization with linear edge-based pricing, where the edge prices are the energy-per-bits of the corresponding physical broadcast links. This paper also investigates minimum energy multicasting with routing. Due to the linearity of the pricing scheme, the minimum energy-per-bit for routing is achievable by using a single distribution tree. A characterization of the admissible rate region for routing with a single tree is presented. The minimum energy-per-bit for multicasting with routing is found by an integer linear program. We show that the relaxation of this integer linear program, studied earlier in the Steiner tree literature, can now be interpreted as the optimization for minimum energy multicasting with network coding. In short, this paper presents a unifying study of minimum energy multicasting with network coding and routing.
•70 studies used interventions to reduce the VPD burden among migrants and refugees.•Interventions focused at the individual, community, provider and/or system level.•Effective, interventions were ...designed to overcome barriers to accessing services.•Cultural strategies addressed the heterogeneity of migrant and refugee communities.•Interventions should be tailored to address migrants’ specific needs.•More evaluation research that reflects the current migration context is needed.
Disparities in vaccine-preventable disease (VPD) burden and immunisation coverage between migrants and refugees and their host populations have been described in numerous countries worldwide. Effective strategies are required to reduce the health disparities and immunisation inequities experienced by migrants and refugees.
Using Arksey and O’Malley’s framework, we conducted a scoping review to identify available literature on interventions aimed at reducing VPD burden among migrants and refugees worldwide. We searched for relevant empirical, peer-reviewed literature published in English between 2006 and 2018 using MEDLINE, Ovid EMBASE, CINAHL, Sociological Abstracts, and Web of Science databases. Relevant information from the studies, including intervention type, details and outcomes, were charted in Microsoft Excel and results were summarised using a descriptive analytical method.
Seventy studies met the inclusion criteria. The number of published studies increased over the years. The majority of studies were conducted in high-income countries. More studies were conducted among migrants (not including refugees) (n = 48, 66%) than specifically among refugees (n = 25, 34%). Interventions were implemented in a variety of settings, including health care (n = 31, 42%), community (n = 29, 39%), off-shore (n = 7, 9%), national (n = 4, 5%), school (n = 2, 3%), and workplace (n = 1, 1%). Studies reported interventions focused at the individual (to facilitate uptake of health services) (n = 4, 5%), community (to raise awareness) (n = 25, 34%), provider (to offer health services) (n = 12, 16%) and/or system (to increase compliance with recommendations) (n = 33, 45%) level. To be effective, interventions were designed to overcome commonly identified barriers to accessing services related to language, culture, distance and cost. Engagement with community members and organisations was an effective way to co-design interventions that address migrants’ specific needs.
Studies emphasised the importance of interventions that address the heterogeneity within and between migrant and refugee populations. Considerable variation in practice remains, therefore more evaluation of interventions is needed to inform policy and programme decision-making.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
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