Identification and characterization of protein phosphorylation sites often requires mass spectrometric analysis, which is not trivial or accessible to many laboratories. Here, a targeted strategy to ...mutagenize putative phosphorylation sites within mitogen-activated protein kinase (MAPK) substrates is described. This employs a combination of standard type II with type IIs restriction enzymes to rapidly create individual or multiple phosphorylation site mutant versions of kinase substrates with high efficiency, thereby reducing the cost for screening mutated clones.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK
ABSTRACT Atmosphere is one of the most important noise sources for ground-based cosmic microwave background (CMB) experiments. By increasing optical loading on the detectors, it amplifies their ...effective noise, while its fluctuations introduce spatial and temporal correlations between detected signals. We present a physically motivated 3D-model of the atmosphere total intensity emission in the millimeter and sub-millimeter wavelengths. We derive a new analytical estimate for the correlation between detectors time-ordered data as a function of the instrument and survey design, as well as several atmospheric parameters such as wind, relative humidity, temperature and turbulence characteristics. Using an original numerical computation, we examine the effect of each physical parameter on the correlations in the time series of a given experiment. We then use a parametric-likelihood approach to validate the modeling and estimate atmosphere parameters from the polarbear-i project first season data set. We derive a new 1.0% upper limit on the linear polarization fraction of atmospheric emission. We also compare our results to previous studies and weather station measurements. The proposed model can be used for realistic simulations of future ground-based CMB observations.
1 WHO/FAO/OIE Collaborating Centre for Reference and Research on Leptospirosis, Western Pacific Region, Communicable Diseases, Queensland Health Forensic and Scientific Services, Brisbane, Australia
...2 Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang, Selangor, Malaysia
3 Bacterial Zoonoses Branch, Coordinating Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA
4 Virology Department, Communicable Diseases, Queensland Health Forensic and Scientific Services, Brisbane, Australia
Correspondence Lee D. Smythe Lee_smythe{at}health.qld.gov.au
A single Leptospira strain (designated Bejo-Iso9 T ) was isolated from a soil sample taken in Johor, Malaysia. The isolate showed motility and morphology typical of the genus Leptospira under dark-field microscopy. Cells were found to be 10–13 µm in length and 0.2 µm in diameter, with a wavelength of 0.5 µm and an amplitude of approximately 0.2 µm. Phenotypically, strain Bejo-Iso9 T grew in Ellinghausen–McCullough–Johnson–Harris medium at 13, 30 and 37 °C, and also in the presence of 8-azaguanine. Serologically, strain Bejo-Iso9 T produced titres towards several members of the Tarassovi serogroup, but was found to be serologically unique by cross-agglutinin absorption test and thus represented a novel serovar. The proposed name for this serovar is Malaysia. Phylogenetic analysis of 16S rRNA gene sequences placed this novel strain within the radiation of the genus Leptospira , with sequence similarities within the range 90.4–99.5% with respect to recognized Leptospira species. DNA–DNA hybridization against the three most closely related Leptospira species was used to confirm the results of the 16S rRNA gene sequence analysis. The G+C content of the genome of strain Bejo-Iso9 T was 36.2 mol%. On the basis of phenotypic, serological and phylogenetic data, strain Bejo-Iso9 T represents a novel species of the genus Leptospira , for which the name Leptospira kmetyi sp. nov. is proposed. The type strain is Bejo-Iso9 T (=WHO LT1101 T =KIT Bejo-Iso9 T ).
Abbreviations: CAAT, cross-agglutinin absorption test; EMJH, Ellinghausen–McCullough–Johnson–Harris; MAT, microscopic agglutination test
The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA, DNA gyrase subunit B and RNA polymerase subunit B gene sequences of Leptospira kmetyi serovar Malaysia strain Bejo-Iso9 T are AB279549 , AB279550 and AB291211 , respectively.
Supplementary tables are available with the online version of this paper.
Exogenous ethylene inhibited nodulation on the primary and lateral roots of pea, Pisum sativum L. cv Sparkle. Ethylene was more inhibitory to nodule formation than to root growth; nodule number was ...reduced by half with only 0.07 microliters/L ethylene applied continually to the roots for 3 weeks. The inhibition was overcome by treating roots with 1 micromolars Ag+ an inhibitor of ethylene action. Exogenous ethylene also inhibited nodulation on sweet clover (Melilotus alba) and on pea mutants that are hypernodulating or have ineffective nodules. Exogenous ethylene did not decrease the number of infections per centimeter of lateral pea root, but nearly all of the infections were blocked when the infection thread was in the basal epidermal cell or in the outer cortical cells.
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