Silver cluster-assembled materials (SCAMs), by virtue of their tunable structure, accessible surface area and excellent stability, hold great promise as highly efficient catalysts. Herein, we report ...a new SCAM Ag12(S t Bu)6(CF3COO)3(TPyP) n (denoted as Ag12TPyP) composed of a Ag12 chalcogenolate cluster core stabilized by porphyrinic ligands. Ag12TPyP showed superior sulfur mustard simulant (2-chloroethyl ethyl sulfide, CEES) degradation efficiency and achieved a half lifetime (t 1/2) of 1.5 min with 100% selectivity. The experimental results demonstrated that synergistic effects between the silver cluster and photosensitizer ligand promote the efficiency of the generation of singlet oxygen (1O2), which accelerates the decontamination rate. Additionally, benefiting from strong affinity between the silver cluster and CEES, Ag12TPyP exhibits a CEES uptake of 74.2 mg g–1. This work demonstrates that SCAMs offer a new route to the rational design of novel materials for the detoxification of mustard gas.
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IJS, KILJ, NUK, PNG, UL, UM
Motivated by recent experimental developments of graphitic-CN (g-CN) sheets, we investigate the suitability of hydrogen storage on Li decorated g-CN
via
first-principles calculations. We find that ...the binding energies of Li atoms are very large, ranging from 2.70 to 4.73 eV, which are significantly higher than the cohesive energy of bulk Li. Lithium atoms therefore tend to form 2D rather than 3D patterns on g-CN, promoting reversible hydrogen adsorption and desorption. Remarkably, the average adsorption energy of H
2
molecules falls in the 0.14-0.23 eV range, and the Li decorated CN shows a high theoretical gravimetric density of 10.81 wt%, which is favorable for massive hydrogen storage. Our results suggest that the Li decorated CN could be a promising hydrogen storage material under realistic conditions.
Motivated by recent experimental developments of graphitic-CN (g-CN) sheets, we investigate the suitability of hydrogen storage on Li decorated g-CN
via
first-principles calculations.
The primary goal of the Internet of things(IoT) is to provide people with anywhere services in real life. But intelligent IoT shouldn’t only provide services, but also consider how to allocate ...heterogeneous resources reasonably, which has become a very challenging problem. To obtain the best resource allocation scheme, it is crucial to minimize the service cost and service time. Since the two objectives are contradictory, we have modelled IoT services as a dynamic multi-objective optimization problem. Then a dynamic multi-objective evolutionary algorithm for dynamic IoT services(dMOEA/DI) is proposed. In dMOEA/DI, we have designed operators such as the appropriate encoding method, dynamic detection operator, filtering strategy, differential evolution, and polynomial mutation. Based on the single service strategy and collaborative service strategy, experimental research is performed on the agricultural IoT services with dynamic requests under different distributions. The simulation experimental results prove that dMOEA/DI performs better than the contrasted algorithms on the IoT service optimization problems.
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CEKLJ, EMUNI, FIS, FZAB, GEOZS, GIS, IJS, IMTLJ, KILJ, KISLJ, MFDPS, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
Taurine upregulated gene1 (TUG1) as a 7.1-kb lncRNA, has been shown to play an oncogenic role in various cancers. However, the biological functions of lncRNA TUG1 in small cell lung cancer (SCLC) ...remain unknown. The aim of this study is to explore the roles of TUG1 in cell growth and chemoresistance of SCLC and its possible molecular mechanism.
The expression of TUG1 in thirty-three cases of SCLC tissues and SCLC cell line were examined by quantitative RT-PCR (qRT-PCR). The functional roles of TUG1 in SCLC were demonstrated by CCK8 assay, colony formation assay, wound healing assay and transwell assay, flow cytometry analysis and in vivo study through siRNA or shRNA mediated knockdown. Western blot assays were used to evaluate gene and protein expression in cell lines. Chromatin immunoprecipitation (ChIP) and RNA binding protein immunoprecipitation (RIP) were performed to confirm the molecular mechanism of TUG1 involved in cell growth and chemoresistance of small cell lung cancer.
We found that TUG1 was overexpressed in SCLC tissues, and its expression was correlated with the clinical stage and the shorter survival time of SCLC patients. Moreover, downregulation of TUG1 expression could impair cell proliferation and increased cell sensitivity to anticancer drugs both in vitro and in vivo. We also discovered that TUG1 knockdown significantly promoted cell apoptosis and cell cycle arrest, and inhibited cell migration and invasion in vitro . We further demonstrated that TUG1 can regulate the expression of LIMK2b (a splice variant of LIM-kinase 2) via binding with enhancer of zeste homolog 2 (EZH2), and then promoted cell growth and chemoresistance of SCLC.
Together, these results suggested that TUG1 mediates cell growth and chemoresistance of SCLC by regulating LIMK2b via EZH2.
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DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Comparison of the degradation mechanisms of RhB over g-C3N4 in the absence (a) and presence (b) of acid.
•Degradation of RhB over g-C3N4 was greatly enhanced in the presence of acid.•O2− is the ...predominant ROSs responsible for the efficient degradation of RhB.•Acidification of g-C3N4 results in the formation of a new surface state.•The formed surface state acts as a trapping site for photo-generated electrons.
Low quantum efficiency has hampered the practical application of graphitic carbon nitride (g-C3N4). In this study, the effect of acid (H2SO4 and HF) on the photocatalytic degradation of Rhodamine B (RhB) over g-C3N4 was studied. It was found that the degradation of RhB was greatly enhanced in the presence of acid, and superoxide (O2−) is the predominant reactive oxygen species (ROSs) that are responsible for the efficient degradation of RhB. It is proposed that acidification of g-C3N4 results in the formation of a new surface state, which is 0.3eV below the conduction band position of g-C3N4. The formed surface state can act as a trapping site for photo-generated electrons, which retards the recombination of the electron–hole pairs, enhancing the photocatalytic activity of g-C3N4.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK
Long-term manganese exposure causes a neurodegenerative disorder referred to as manganese poisoning, but the mechanism remains unclear and no specific treatment is available. Oxidative stress is ...widely recognised as one of the main causes of manganese-induced neurotoxicity. In recent years, the role of histone acetylation in neurodegenerative diseases has been widely concerned. curcumin is a natural polyphenol compound extracted from the rhizome of turmeric and exhibits both antioxidant and neuroprotective properties. Therefore, we aimed to investigate whether and how curcumin protects against manganese-induced neurotoxicity from the perspective of histone acetylation, based on the reversibility of histone acetylation modification. In this study, rats were treated with or without curcumin and subjected to long-term manganese exposure. Results that treatment of manganese decreased the protein expression of H3K18 acetylation and H3K27 acetylation at the promoters of oxidative stress-related genes and inhibited the expression of these genes. Nevertheless, curcumin increased the H3K27 acetylation level at the manganese superoxide dismutase (SOD2) gene promoter and promoted the expression of SOD2 gene. Oxidative damage in the rat striatum as well as learning and memory dysfunction were ameliorated after curcumin treatment. Taken together, our results suggest that the regulation of oxidative stress by histone acetylation may be a key mechanism of manganese-induced neurotoxicity. In addition, curcumin ameliorates Mn-induced neurotoxicity may be due to alleviation of oxidative damage mediated by increased activation of H3K27 acetylation at the SOD2 gene promoter.
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•Down-acetylation levels of H3K27 and H3K18 may inhibit the expression of OSR genes.•Curcumin ameliorates Mn-induced neurotoxicity in rat.•Curcumin increases the expression of SOD2 gene by the up-acetylation level of H3K27.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Long‐term arsenic exposure is a worldwide public health problem that causes serious harm to human health. The liver is the main target organ of arsenic toxicity; arsenic induces disruption of the DNA ...damage repair pathway, but its mechanisms remain unclear. In recent years, studies have found that epigenetic mechanisms play an important role in arsenic‐induced lesions. In this study, we conducted experiments in vitro using normal human liver cells (L‐02) to explore the mechanism by which the histone demethylase JHDM2A regulates H3K9 dimethylation (me2) in response to arsenic‐induced DNA damage. Our results indicated that arsenic exposure upregulated the expression of JHDM2A, downregulated global H3K9me2 modification levels, increased the H3K9me2 levels at the promoters of base excision repair (BER) genes (N‐methylpurine‐DNA glycosylase MPG, XRCC1 and poly(ADP‐ribose)polymerase 1) and inhibited their expression levels, causing DNA damage in cells. In addition, we studied the effects of overexpression and inhibition of JHDM2A and found that JHDM2A can participate in the molecular mechanism of arsenic‐induced DNA damage via the BER pathway, which may not be involved in the BER process because H3K9me2 levels at the promoter region of the BER genes were unchanged following JHDM2A interference. These results suggest a potential mechanism by which JHDM2A can regulate the MPG and XRCC1 genes in the process of responding to DNA damage induced by arsenic exposure and can participate in the process of DNA damage repair, which provides a scientific basis for understanding the epigenetic mechanisms and treatments for endemic arsenic poisoning.
Arsenic poisoning can lead to abnormal DNA damage and repair, which is related to the regulation of histone methylation. JHDM2A is the histone demethylase that specifically removes the methyl group from histone lysine H3K9, which is overexpressed in various tumors. After inhibition of JHDM2A, it can increase the expression of H3K9me2 and inhibit the proliferation of cancer cells. Our study shows that arsenic can upregulate JHDM2A and aggravate DNA damage, and JHDM2A inhibition can reduce levels of DNA damage.
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BFBNIB, FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SAZU, SBCE, SBMB, UL, UM, UPUK
Internet of Things (IoT) aims to provide ubiquitous services in real life. When different service requests arrive, how to assign them to proper service providers has become a challenging problem, ...especially in large-scale IoT service circumstances. In order to obtain the best service matching scheme, it is crucial to minimize total service cost and service time. Since both goals are conflicting, we have modeled IoT service as a multiobjective problem. Thus, we propose an improved decomposition-based multiobjective evolutionary algorithm for the IoT service (I-MOEA/D-IoTS). We have designed appropriate operators, such as array encoding, population initialization, Tchebycheff decomposition approach, local improvement, simulated binary crossover, and Gaussian mutation. In order to verify the effectiveness of the proposed algorithm, we apply it in three different scenarios of the agricultural IoT service. The simulation experimental results show that the proposed algorithm can achieve better tradeoff of solutions for IoT service and reduce total service cost and shorten service time.
We evaluated the effects of T helper cell differentiation in a mite-allergic animal model treated with inhaled heparins of different molecular weight.
BALB/c mice were divided into four groups: 1. ...Control, 2. Mite intratracheal (mIT), 3. Inhaled heparin (hIN), 4. Inhaled low-molecular-weight heparin (lmwhIN). Groups 2, 3, and 4 were sensitized twice with Der p allergen subcutaneously on day 1 and day 8. Der p allergen was administered intratracheally on day 15. Groups 3 and 4 were treated with heparin or low-molecular-weight (lmw) heparin intranasally from day 1 to 22. Splenocytes from sacrificed mice stimulated with 16 µg/ml of Der p were cultured for 72 hours. Supernatants of splenocyte were collected to analyze the effect of Interleukin (IL)17-A/F, Interferon(IFN)-γ, IL-4, IL-13, and IL-10. Serum was also collected for Der P-specific IgE level on day 23. Total RNA was extracted from spleen tissue for mRNA expression. Gene expression of Foxp3, IL-10 IFN-γ, GATA3, IL-5, and RORγt were analyzed.
Both hIN and lmwhIN groups had lower serum IgE level than that of the mIT group (both p<0.0001). Both hIN and lmwhIN groups showed significantly decreased transcripts of GATA-3, IFN-γ, IL-5, and RORγt mRNA in their spleen. Regarding the supernatant of splenocyte culture stimulated with Der p, compared with the mIT group, there were significant decreases in IL-17A/F, IFN-γ, IL-4, IL-13, and IL-10 secretion in inhaled hIN and lmwhIN groups.
From this balb/c mice study, the analyses of mRNA and cytokines revealed that both intranasal heparin and lmw heparin treatment decreased the expression of Th1, Th2, and Th17 in spleen. The underlying mechanism(s) warrant further studies.
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DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
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