is a common bacterial genus in marine sediments and deep seas, with a variety of metabolic abilities, suggesting its important roles in the marine biogeochemical cycles. In this study, a novel lytic ...Shewanella phage, vB_SInP-X14, was isolated from the surface coastal waters of Qingdao, China. The vB_SInP-X14 contains a linear, double-strand 36,396-bp with the G + C content of 44.1% and harbors 40 predicted open reading frames. Morphological, growth, and genomic analysis showed that it is the first isolated podovirus infecting
, with a short propagation time (40 min), which might be resulted from three lytic-related genes. Phylogenetic analysis suggested that vB_SInP-X14 could represent a novel viral genus, named Bocovirus, with four isolated but not classified phages. In addition, 14 uncultured viral genomes assembled from the marine metagenomes could provide additional support to establish this novel viral genus. This study reports the first podovirus infecting
, establishes a new interaction system for the study of virus-host interactions, and also provides new reference genomes for the marine viral metagenomic analysis.
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•Eriodictyol has a therapeutic effect on NAFLD.•Eriodictyol improves NAFLD by regulating UBA52-mediated autophagy.•Eriodictyol improves NAFLD by upregulating Nrf2/HO-1 to inhibit ...oxidative stress.•Eriodictyol improves NAFLD by inhibiting NF-κB to relieve inflammation.
Eriodictyol (Eri) is a widely distributed flavonoid compound. We explored the effect of Eri on non-alcoholic fatty liver disease (NAFLD) and the underlying mechanism. Eri reduced hepatic lipid accumulation and alanine aminotransferase, aspartate aminotransferase, cholesterol, and triglyceride levels in high-fat diet-induced NAFLD mice. Proteomics analysis showed that Eri regulated oxidative stress- and autophagy-related signaling pathways. Eri treatment upregulated MAP1LC3 (LC3)II/I, nuclear factor E2-related factor (Nrf2), and heme oxygenase 1 (HO-1), downregulated Ubiquitin A-52 residue ribosomal protein fusion product 1 (UBA52) and SQSTM1 (p62), inhibited p-nuclear factor-κB (p-NF-κB), and reduced tumor necrosis factor-α (TNF-α) and interleukin 6 (IL-6) release. UBA52 knockdown or Eri treatment promoted autophagy in oil acid-induced HepG2 cells. UBA52 knockdown combined with Eri treatment produced a more prominent effect than either of the treatments alone. Eri also significantly ameliorated hepatic lipid accumulation in db/db mice. Thus, Eri ameliorated NAFLD by downregulating UBA52 to promote autophagy and activated Nrf2/HO-1 to inhibit oxidative stress. Eri might be a potential compound for treating NAFLD.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
The scaling law of divertor heat flux width is one of the key topics of magnetic confinement fusion, which is almost inversely proportional to the poloidal magnetic field on some opened divertor ...tokamaks. This work focuses on the scaling laws of the closed divertor heat flux width in the HL-2A tokamak under different discharge conditions, such as the Ohmic, L- and H-modes. The results indicate that there are basic similarities of the scaling laws of the heat flux width between the opened and closed divertors. However, a larger spreading width in the private flux region is found, which is relevant to a small expansion factor of the magnetic flux in the closed divertor.
Abstract
Applications of organic–inorganic formamidinium (FA) lead triiodide (FAPbI
3
) perovskites in high-efficiency solar cells often suffer from spontaneous α-to-δ phase transitions. However, ...current efforts to inhibit this phenomenon based on simple cation and anion alloying strategies continue to suffer from unintended consequences, such as unfavorable shifts in the bandgap energy and unwanted phase separation during operation. The present work compares the effects of bromine (Br) anion and dimethylamine (DMA) cation alloying on the structure and properties of FAPbI
3
perovskite in detail. DMA-incorporated FAPbI
3
perovskites show significantly improved structural stability and photovoltaic performance, while the inherent bandgap energy of the original material is maintained. Rigorous analyses demonstrate that the relatively large size and free isotropic motion of the incorporated DMA cations constrain the dynamic space of neighboring FA cations, which increases the degree to which the FA cations interact with the inorganic lattice and therefore stabilizes the PbI
6
lattice structure without significant lattice distortion. Hence, this work demonstrates an efficient method for improving the phase stability of FAPbI
3
perovskite materials while providing a plausible molecular mechanism for the stability engendered by the alloying of DMA and FA.
Coronary heart disease is a highly prevalent inflammatory disease caused by coronary atherosclerosis. Numerous studies have revealed that perivascular adipose tissue is closely associated with ...atherosclerosis. Here, we conducted a comprehensive analysis of long non-coding RNAs and mRNAs differentially expressed in perivascular adipose tissue in patients with coronary heart disease.BackgroundCoronary heart disease is a highly prevalent inflammatory disease caused by coronary atherosclerosis. Numerous studies have revealed that perivascular adipose tissue is closely associated with atherosclerosis. Here, we conducted a comprehensive analysis of long non-coding RNAs and mRNAs differentially expressed in perivascular adipose tissue in patients with coronary heart disease.We conducted Gene Ontology term and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses of the differentially expressed genes. Furthermore, single sample gene set enrichment analysis, immune infiltration analysis, and co-expression analysis of differentially expressed long non-coding RNAs and immune gene sets were performed. Finally, the starBase and miRTarBase databases were used to construct a competing endogenous RNA network.MethodsWe conducted Gene Ontology term and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses of the differentially expressed genes. Furthermore, single sample gene set enrichment analysis, immune infiltration analysis, and co-expression analysis of differentially expressed long non-coding RNAs and immune gene sets were performed. Finally, the starBase and miRTarBase databases were used to construct a competing endogenous RNA network.The results show that aortic perivascular adipose tissue has higher inflammation and immune infiltration levels in patients with coronary heart disease. Dysregulated long non-coding RNAs may be related to immunity, inflammation, and hypoxia.ResultsThe results show that aortic perivascular adipose tissue has higher inflammation and immune infiltration levels in patients with coronary heart disease. Dysregulated long non-coding RNAs may be related to immunity, inflammation, and hypoxia.The findings of this study provide new insights into atherosclerosis and coronary heart disease.ConclusionsThe findings of this study provide new insights into atherosclerosis and coronary heart disease.
Hawthorn, one of the widely‐used Chinese herbal medicines, has been used to treat blood stasis syndrome in the clinic, but its blood‐activating components are unclear. This study combined the ...ultra‐high‐performance liquid chromatography‐quadruple exactive‐orbitrap mass spectrometry with chemometrics to identify the blood‐activating components of hawthorn. Different polar fractions of hawthorn aqueous extracts were extracted and mixed to prepare 14 samples. The contents of 25 chemical components for 14 samples were determined by the proposed quantitative method which was validated in terms of linearity, precision, stability, repeatability, and recovery, while the blood‐activating effect was evaluated by measuring the whole blood viscosity, plasma viscosity, and plasma fibrinogen levels. Then the partial least squares model was established on the spectrum‐effect relationship. The result showed that vitexin‐2″‐O‐rhamnoside, rutin, citric acid, malic acid, gallic acid, and fumaric acid could reduce the whole blood viscosity, plasma viscosity, and plasma fibrinogen levels in blood stasis model rats, and these components were the blood‐activating components of hawthorn. This study provided a scientific basis for clarifying the blood‐activating components of hawthorn, and the spectrum‐effect approach proved to be an effective approach to discovering the bioactive components of Chinese herbal medicines.
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BFBNIB, FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SAZU, SBCE, SBMB, UL, UM, UPUK
Formamidinium (CH(NH2)2, FA) lead halide perovskites exhibit excellent optoelectronic properties for the applications of light‐emitting diodes and lasers. However, the reported formamidinium lead ...bromide (FAPbBr3) perovskite‐based lasers mainly depend on the external feedback or Fabry–Pérot mode cavities and suffer from moderate quality (Q) factors. Herein, solution‐processed square and quasi‐circular FAPbBr3 microdisks are developed to achieve room‐temperature naturally facet‐formed whispering gallery mode (WGM) lasers. These microdisks exhibit relatively high Q factor and tunable laser modes with threshold of 10–70 µJ cm−2 under 400 nm excitation. Although the synthesized circular microdisk is not regular enough, it still shows higher Q factor of 3455 than that of square microdisk due to the less boundary wave and pesudointegrable leakage. This simple and low‐cost solution processed method can tackle the complicated and expensive conventional preparation techniques of circular microdisks. Power dependent photoluminescence decay transients indicate that the band edge emission of the FAPbBr3 microdisks originates from free carrier recombination. In addition, these microdisks show high photostability and that room temperature lasing can sustain over 50 min. These results suggest that FAPbBr3 microdisks can serve as promising WGM lasers with excellent light emission capability and stability toward practical optoelectronic applications.
Solution‐processed square and quasi‐circular formamidinium lead bromide microdisks are developed to achieve room‐temperature naturally facet‐formed whispering gallery mode lasers. Although the synthesized circular microdisk is not regular enough, it still shows higher quality (Q) factor of 3455 than that of square microdisk. These microdisks exhibit tunable laser modes with threshold of 10–70 µJ cm−2 under 400 nm excitation.
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BFBNIB, FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SAZU, SBCE, SBMB, UL, UM, UPUK
Marinobacter is the abundant and important algal-associated and hydrocarbon biodegradation bacteria in the ocean. However, little knowledge about their phages has been reported. Here, a novel ...siphovirus, vB_MalS-PS3, infecting
Marinobacter algicola
DG893(T), was isolated from the surface waters of the western Pacific Ocean. Transmission electron microscopy (TEM) indicated that vB_MalS-PS3 has the morphology of siphoviruses. VB_MalS-PS3 was stable from −20 to 55°C, and with the latent and rise periods of about 80 and 10 min, respectively. The genome sequence of VB_MalS-PS3 contains a linear, double-strand 42,168-bp DNA molecule with a G + C content of 56.23% and 54 putative open reading frames (ORFs). Nineteen conserved domains were predicted by BLASTp in NCBI. We found that vB_MalS-PS3 represent an understudied viral group with only one known isolate. The phylogenetic tree based on the amino acid sequences of whole genomes revealed that vB_MalS-PS3 has a distant evolutionary relationship with other siphoviruses, and can be grouped into a novel viral genus cluster with six uncultured assembled viral genomes from metagenomics, named here as
Marinovirus.
This study of the Marinobacter phage vB_MalS-PS3 genome enriched the genetic database of marine bacteriophages, in addition, will provide useful information for further research on the interaction between Marinobacter phages and their hosts, and their relationship with algal blooms and hydrocarbon biodegradation in the ocean.
•MAPbBr3 nanocrystals (NCs) can modulate the growth of quasi-2D perovskite films.•NCs-mediated film exhibits smooth surface morphology and uniform PL emission.•The carrier funneling is enhanced in ...the NCs-mediated film.•NCs-mediated PeLED delivers improved efficiency and stability.
Quasi-2D halide perovskites have recently emerged as a promising family of semiconducting materials for light-emitting applications. However, these materials usually consist of mixed 2D-3D perovskite phases with rarely controlled distributions when processed into thin films. Such film structures can be detrimental to carrier transport and transfer, limiting the electroluminescent properties and light-emitting device performance. In this work, we tailor the solution crystallization of quasi-2D perovskites with a nanocrystals-mediation strategy, which leads to high-quality, low-defect-density quasi-2D perovskite films with uniformly mixed 2D-3D perovskite phases. Mechanistic insights gained from ultrafast spectroscopic studies reveal a highly enhanced carrier funnelling process in such nanocrystals-mediated films, which results in a threefold increase in the electroluminescence efficiency compared with the pristine thin pristine films. This work demonstrates the promise of a potentially versatile approach in using nanocrystals to manipulate the functional properties of quasi-2D perovskite thin films.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
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