Backgrounds
Colorectal carcinoma (CRC) is a common malignant tumor. Increasing evidences indicated that CRC showed a resistance to 5‐fluorouracil (5‐FU) and further resulted in a poor prognosis. In ...this study, we aim to investigate the effect of long noncoding RNA nuclear paraspeckle assembly transcript 1 (LncRNA NEAT1) on cell viability, sensitivity to 5‐FU, and autophagy of CRC cell lines.
Methods
MTT (3‐(4,5‐dimethyl‐2‐thiazolyl)‐2,5‐diphenyl‐2‐Htetrazolium bromide) was used to detect cell viability, immunofluorescent staining was used to detect autophagy puncta, and luciferase reporter system was used to determine binding ability between miR‐34a and NEAT1 or putative targets. Additionally, indicated mRNAs and protein expressions were determined by qRT‐PCR or western blotting, respectively.
Results
We found that NEAT1 expression was increased in CRC tissues and cells, which showed a negative correlation with miR‐34a expression. In addition, NEAT1 knockdown noticeably inhibited the proliferation of CRC cells and enhanced 5‐FU sensitivity. It revealed that NEAT1 knockdown suppressed the LC3 puncta and the expressions of Beclin‐1, ULK1, and ratio of LC3II/I. Overexpression of miR‐34a showed similar trends with NEAT1 knockdown. miR‐34a was validated to target the putative binding sites in 3′‐UTR of HMGB1, ATG9A, and ATG4B, which are involved in the activation of autophagy. Inhibition of miR‐34a or overexpression of HMGB1 could effectively reverse elevated 5‐FU sensitivity upon NEAT1 knockdown. In addition, 3‐MA reversed NEAT1 overexpression‐induced resistance in HT29 cells.
Conclusion
These findings indicate that LncRNA NEAT1 could target miR‐34a and promote autophagy to facilitate 5‐FU chemoresistance in CRC.
NEAT1 knockdown attenuates autophagy to elevate 5‐FU sensitivity in CRC cell lines. NEAT1 is an endogenous compete RNA of miR‐34a in CRC.
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FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SBCE, SBMB, UL, UM, UPUK
Gel polymer electrolyte (GPE), possessing a polymer matrix and embedded liquid electrolyte, is a critical component of lithium-ion batteries. To enhance GPE performance, in this work a ...self-crosslinkable poly(vinylidene difluoride) (PVdF) graft copolymer possessing Meldrum's acid (MA) groups as reactive sites has been prepared with atom transfer radical polymerization and utilized as a polymer matrix for GPEs. Compared to neat PVdF based GPEs, incorporation of MA groups to PVdF chains simultaneously brings crosslinking characteristic and high polarity to PVdF chains so as to enhance the thermal stability, mechanical strength, and polymer matrix-electrolyte affinity of the corresponding GPEs. The prepared GPE has an ionic conductivity of about 1.48 mS cm−1, which is higher than the value of 0.98 mS cm−1 measured on commercial Celgard 2325® separator. Moreover, good Li-ion battery performance, including high discharge capacity (136 mAh g−1 at 0.2 C) and high capacity retention at high C-rates (66% at 10 C and 50% at 15 C), has been recorded with the prepared GPE based LIBs, demonstrating the application potential of the developed GPE for fast-charging lithium-ion batteries.
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•Crosslinkable PVdF is prepared with atom transfer radical polymerization.•Crosslinked PVdF based fiber mat is obtained as a matrix of gel polymer electrolytes.•The matrix shows attractive thermal stability and electrolyte compatibility.•The battery employing the gel polymer electrolyte retains 50% capacity at high charge rate (15C).
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK, ZRSKP
The biochemical response to ursodeoxycholic acid (UDCA) in primary biliary cirrhosis is a strong predictor of long‐term outcome and thus facilitates the rapid identification of patients needing new ...therapeutic approaches. Numerous criteria for predicting outcome of treatment have been studied based on biochemical response to UDCA at 1 year. We sought to determine whether an earlier biochemical response at 3 or 6 months could as efficiently identify patients at risk of poor outcome, as defined by liver‐related death, liver transplantation, and complications of cirrhosis. We analyzed the prospectively collected data of 187 patients with a median follow‐up of 5.8 years (range, 1.3‐14 years). The survival rates without adverse outcome at 5 years and 10 years were 86% and 63%. Under UDCA therapy, laboratory liver parameters experienced the most prominent improvement in the first 3 months (P < 0.0001) and then stayed relatively stable for the following months. The Paris, Barcelona, Toronto, and Ehime definitions, but not the Rotterdam definition, applied at 3, 6, and 12 months significantly discriminated the patients in terms of long‐term outcome. Compared with biochemical responses evaluated after 1 year of UDCA therapy, biochemical responses at the third month demonstrated higher positive predictive value (PPV) but lower negative predictive value (NPV) and increased negative likelihood ratio (NLR) by all definitions; biochemical responses at the sixth month showed higher or the same PPV and NPV and lower NLR by all definitions. Conclusion: For the previously published criteria, biochemical responses at the sixth month can be used in place of those evaluated after 1 year of UDCA therapy. Our findings justify a more rapid identification of patients who need new therapeutic approaches. (HEPATOLOGY 2013)
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BFBNIB, FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SBCE, SBMB, UL, UM, UPUK
In this letter, we present a 1.55-<inline-formula> <tex-math notation="LaTeX">\mu \text{m} </tex-math></inline-formula> DFB laser that possesses characteristics including high power, low noise, and ...high-speed direct modulation capabilities across a wide temperature range. The DFB laser demonstrates an optical power of 125.76-mW and sustains power exceeding 45-mW, with an SMSR surpassing 45-dB at 85°C. It exhibits the ability to modulate signals at frequencies exceeding 5.26-GHz and transmits a clear 10-Gb/s eye pattern. The RIN maintains as low as -153.51-dB/Hz within the dynamic range of 0-10-GHz, and even with the operating temperature increases, it still stays impressively low at -141.47-dB/Hz. Consequently, these outcomes indicate the 1.55-<inline-formula> <tex-math notation="LaTeX">\mu \text{m} </tex-math></inline-formula> DFB laser offers several advantages, reinforcing its potential as a promising device for satellite optical links.
Organic light-emitting diode (OLED) is an attractive technology for display applications in high-end electronics. Tetradentate Pt(
ii
) complexes are phosphorescence emitters of great importance for ...OLED fabrication. A novel series of N-heterocyclic carbene (NHC), benzocarbene (Ph/NHC) and pyridinocarbene (Py/NHC)-based tetradentate Pt(
ii
) complexes with fused 5/6/6 metallocycles was designed and developed. Their electrochemical and photophysical properties were systematically studied through experimental and theoretical investigations, which were greatly affected by the NHC, Ph/NHC and Py/NHC. Pt(NHC-1) shows a dominant emission peak at 509 nm with a quantum efficiency of 40% and an excited lifetime of 1.8 μs in a 5 wt% doped PMMA film at room temperature. A Pt(NHC-1)-doped green OLED using 26mCPy as the host material demonstrated a very high
L
max
of 64 416 cd m
−2
, which was a record-high value for the reported Pt(
ii
) complex-based phosphorescent OLED. The OLED also achieved a peak EQE of 13.1% with small efficiency roll-off, which still maintained EQEs of 12.8%, 11.6% and 8.6% at a high brightness of 100, 1000 and 10 000 cd m
−2
.
A novel series of NHCs-based tetradentate Pt(
II
) complexes were designed and developed. Pt(NHC-1)-based green OLED achieved a maximum luminescence of 64 416 cd m
−2
, which was a record-high value for reported Pt(
II
) complexes-based phosphorescent OLEDs.
Abstract
Background
Periodontal ligament stem cells (PDLSCs) are the most potential cells in periodontal tissue regeneration and bone tissue regeneration. Our prior work had revealed that WD ...repeat‐containing protein 72 (WDR72) was crucial for osteogenic differentiation of PDLSCs. Here, we further elucidated its underlying mechanism in PDLSC osteogenic differentiation.
Methods
Human PDLSCs, isolated and identified by flow cytometry, were prepared for osteogenic differentiation induction. Levels of WDR72, long non‐coding RNA X‐Inactive Specific Transcript (XIST), upstream stimulatory factor 2 (USF2), and osteogenic marker genes (Runx2, Osteocalcin, and Collagen I) in human PDLSCs and clinical specimens were detected by RT‐qPCR. Protein expressions of WDR72, Runx2, Osteocalcin, and Colla1 were tested by Western blot. The interactions among the molecules were verified by RIP, RNA pull‐down, ChIP, and luciferase reporter assays. Osteogenic differentiation was evaluated by alkaline phosphatase (ALP) and alizarin red staining (ARS).
Results
WDR72 was decreased in periodontal tissues of periodontitis patients, and overexpression reversed TNF‐α‐mediated suppressive effects on PDLSC osteogenic differentiation. Mechanically, XIST recruited the enrichment of USF2 to the WDR72 promoter region, thereby positively regulating WDR72. WDR72 silencing overturned XIST‐mediated biological effects in PDLSCs.
Conclusion
WDR72, regulated by the XIST/USF2 axis, enhances osteogenic differentiation of PDLSCs, implying a novel strategy for alleviating periodontitis.
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FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SBCE, SBMB, UL, UM, UPUK
Spatially confined photocatalysis has emerged as a viable strategy for the intensification of various redox reactions, but the influence of confined structure on reaction behavior is always ...overlooked in gas‐solid reactions. Herein, we report a nanomembrane with confining Cs3Bi2Br9 nanocrystals inside vertical channels of porous insulated silica thin sheets (CBB@SBA(⊥)) for photocatalytic nitric oxide (NO) abatement. The ordered one‐dimensional (1D) pore channels with mere 70 nm channel length provide a highly accessible confined space for catalytic reactions. A record‐breaking NO conversion efficiency of 98.2 % under a weight hourly space velocity (WHSV) of 3.0×106 mL g−1 h−1, as well as exceptionally high stability over 14 h and durability over a wide humidity range (RH=15–90 %) was realized over SBA(⊥) confined Cs3Bi2Br9, well beyond its nonconfined analogue and the Cs3Bi2Br9 confine in Santa Barbara Amorphous (SBA‐15). Mechanism studies suggested that the insulated pore channels of SBA(⊥) in CBB@SBA(⊥) endow concentrated electron field and enhanced mass transfer that render high exposure of reactive species and lower reaction barrier needs for ⋅O2− formation and NO oxidation, as well as prevents structural degradation of Cs3Bi2Br9. This work expands an innovative strategy for designing efficient photocatalysts for air pollution remediation.
Nanoconfined photocatalysis promises to achieve challenging chemical transformation, but the influence of confined structure has been ignored. Here, we propose a confinement model by encapsulating Cs3Bi2Br9 into one‐dimensional insulated channels of porous silica nanomembranes, rendering the concentrated electric field, high exposure of ⋅O2− and enhanced mass transfer for exceptional photocatalytic nitric oxide (NO) abatement.
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BFBNIB, FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SBCE, SBMB, UL, UM, UPUK
Several in vitro and in vivo studies have demonstrated the toxicity of perfluoroalkyl and polyfluoroalkyl substances (PFASs) alternatives, however, relevant epidemiological findings remain to be ...performed. In addition, the association between PFASs alternatives and blood pressure has not been explored. To address this gap, we quantified serum levels of alternatives and legacy PFAS in 1273 healthy Chinese, aged 34–94 years, from “isomers of C8 health project”. Our results showed that an increase of serum PFASs levels was correlated with elevated blood pressure and higher prevalence of hypertension: per natural log unit (ng/mL) increase of 6:2 chlorinated polyfluorinated ether sulfonic acids (Cl-PFESA) elevated 1.31 (95%CI: 0.13, 2.50) mmHg of diastolic pressure (DBP). Adjusted odds ratios (aORs) for hypertension with per natural log increase of 6:2 and 8:2 Cl-PFESA were 2.57 (95%CI: 1.86, 3.56) and 1.18 (95%CI: 1.06, 1.32), respectively. When stratified by sex, the effects of PFASs alternatives on increased blood pressure and hypertension were stronger in women. Meanwhile, the association between 6:2 Cl-PFESA (aOR = 6.81; 95%CI: 3.54, 13.09) and hypertension was stronger than perfluorooctanoic acid (PFOA) (aOR = 2.32, 95%CI: 1.38, 3.91) in women. In conclusion, our pilot study demonstrates that serum concentrations of PFASs alternatives are positively associated with blood pressure. Moreover, women seem to be more susceptible, and alternatives exhibited stronger effects than legacy PFASs.
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•A large population-based study was conducted to assess associations between PFASs alternatives exposure and blood pressure.•Exposure to PFASs alternatives increases the estimates of blood pressure and the odds ratio of hypertension.•PFASs alternatives exhibit stronger effects than legacy PFASs on blood pressure, and women are at greater health risk.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Acral melanoma is a dismal subtype of melanoma occurring in glabrous acral skin, and has a higher incidence in East Asians. We perform single-cell RNA sequencing for 63,394 cells obtained from 5 ...acral and 3 cutaneous melanoma samples to investigate tumor heterogeneity and immune environment. We define 5 orthogonal functional cell clusters that are involved in TGF-beta signaling, Type I interferon, Wnt signaling, Cell cycle, and Cholesterol efflux signaling. Signatures of enriched TGF-beta, Type I interferon, and cholesterol efflux signaling are significantly associated with good prognosis of melanoma. Compared with cutaneous melanoma, acral melanoma samples have significantly severe immunosuppressive state including depletion of cytotoxic CD8+ T cells, enrichment of Treg cells, and exhausted CD8+ T cells. PD1 and TIM-3 have higher expression in the exhaustive CD8+ T cells of acral melanoma. Key findings are verified in two independent validation sets. This study contributes to our better understanding of acral melanoma.
Turning insulating silk fibroin materials into conductive ones turns out to be the essential step toward achieving active silk flexible electronics. This work aims to acquire electrically conductive ...biocompatible fibers of regenerated Bombyx mori silk fibroin (SF) materials based on carbon nanotubes (CNTs) templated nucleation reconstruction of silk fibroin networks. The electronical conductivity of the reconstructed mesoscopic functional fibers can be tuned by the density of the incorporated CNTs. It follows that the hybrid fibers experience an abrupt increase in conductivity when exceeding the percolation threshold of CNTs >35 wt%, which leads to the highest conductivity of 638.9 S m−1 among organic‐carbon‐based hybrid fibers, and 8 times higher than the best available materials of the similar types. In addition, the silk‐CNT mesoscopic hybrid materials achieve some new functionalities, i.e., humidity‐responsive conductivity, which is attributed to the coupling of the humidity inducing cyclic contraction of SFs and the conductivity of CNTs. The silk‐CNT materials, as a type of biocompatible electronic functional fibrous material for pressure and electric response humidity sensing, are further fabricated into a smart facial mask to implement respiration condition monitoring for remote diagnosis and medication.
A new conceptual silk meso‐fibrous material for biocompatible electronic applications is developed by carbon nanotubes meso reconstruction. It can be adopted to fabricate various fibrous sensors, i.e., electronic humidity sensors. In combination with internet of things (IoTs) and artificial intelligence technologies, a remote respiratory condition monitoring and diagnosis can be achieved.
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BFBNIB, FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SBCE, SBMB, UL, UM, UPUK
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