Radical cascade cyclization of β,γ‐unsaturated hydrazones/oximes has recently emerged as an efficient and powerful protocol for the construction of diverse and valuable functionalized pyrazolines and ...isoxazolines. In this review, three catalytic ways of radical cascade cyclization of β,γ‐unsaturated hydrazones/oximes are summarized and classified; these are transition‐metal‐catalyzed systems, transition‐metal‐free systems, and photo‐/electrocatalytic systems, respectively. Through these methods, various functional groups are installed on the pyrazoline and isoxazoline skeletons to enrich the small organic molecule library.
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BFBNIB, FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SAZU, SBCE, SBMB, UL, UM, UPUK
A convenient radical cascade cyclization of aniline‐linked 1,7‐enynes with 1,3‐dicarbonyl compounds was developed to synthesize cyclopentacquinolines in the presence of AgNO3/K2S2O8. A wide range of ...cyclopentacquinolines were prepared in moderate to excellent yields.
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BFBNIB, FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SAZU, SBCE, SBMB, UL, UM, UPUK
We generated PUMCi005-A, an induced pluripotent stem cell (iPSC) line, from dermal fibroblasts of a 32-year-old female Perrault syndrome patient with double heterozygous (794 G > A and 1181 G > A) ...mutations in the TWNK gene using Sendai viral delivery of OCT4, SOX2, KLF4, and c-MYC. The PUMCi005-A iPSC line carried the TWNK mutations, displayed typical iPSC morphology, expressed pluripotent stem cell markers, did not have integration of Sendai virus, and exhibited a normal karyotype and differentiation into three germ layers.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
A KD-control human induced pluripotent stem cells (iPSCs) line (PUMCi002-A) was generated from dermal fibroblasts of a Krabbe patient’s father with a c.461C>A mutation in Galactocerebrosidase (GALC) ...gene. The pluripotency, in vitro differentiation potential and karyotype stability of generated iPSC line were analyzed and confirmed. This cell line can be exploited as a control iPSC line to better understand the mechanisms involved in GALC-associated Krabbe disease and provide plausible new therapeutic directions.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
The aim of this study is to evaluate an AAV vector that can selectively target breast cancer cells and to investigate its specificity and anti-tumor effects on breast cancer cells both in vitro and ...in vivo, offering a new therapeutic approach for the treatment of EpCAM-positive breast cancer.
In this study, a modified AAV2 viral vector was used, in which EpCAM-specific DARPin EC1 was fused to the VP2 protein of AAV2, creating a viral vector that can target breast cancer cells. The targeting ability and anti-tumor effects of this viral vector were evaluated through in vitro and in vivo experiments.
The experimental results showed that the AAV2M
virus could specifically infect EpCAM-positive breast cancer cells and accurately deliver the suicide gene HSV-TK to tumor tissue in mice, significantly inhibiting tumor growth. Compared to the traditional AAV2 viral vector, the AAV2M
virus exhibited reduced accumulation in liver tissue and had no impact on tumor growth.
This study demonstrates that AAV2M
is a gene delivery vector capable of targeting breast cancer cells and achieving selective targeting in mice. The findings offer a potential gene delivery system and strategies for gene therapy targeting EpCAM-positive breast cancer and other tumor types.
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DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Removal of ciprofloxacin (CIP) from wastewater is of significance to protect the environment. In this study, phosphomolybdic acid (H
3
PMo
12
O
40
) -modified metal–organic framework (UiO-66-NH
2
) ...materials were prepared by the solvothermal method for the removal of CIP from wastewater. The adsorption performance of H
3
PMo
12
O
40
@ UiO-66-NH
2
on CIP was remarkably superior to that of pure UiO-66-NH
2
. Several experiments with altered conditions, including CIP concentration, contact time, and pH, were explored to understand the adsorption performance of H
3
PMo
12
O
40
@ UiO-66-NH
2
on CIP solutions. The experimental results show that when pH = 7,
T
= 323 K, the amount of H
3
PMo
12
O
40
is 0.14 g, which is the best CIP adsorption condition for H
3
PMo
12
O
40
@UiO-66-NH
2
, and the adsorption amount is 164.28 mg g
−1
. The adsorption kinetics and isotherms of CIP molecules on the modified UiO-66-NH
2
adsorbent showed that they were basically in agreement with the pseudo-second-order kinetic model and Langmuir model, respectively. Thermodynamic parameters indicate that the adsorption process is spontaneous endothermic. After five cycles of experiments, the adsorbent can still remove 81% of the CIP, indicating that it is reproducible and practical for CIP. Hence, the H
3
PMo
12
O
40
@ UiO-66-NH
2
has potential application value as an antibiotic’s adsorbent.
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EMUNI, FIS, FZAB, GEOZS, GIS, IJS, IMTLJ, KILJ, KISLJ, MFDPS, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
An efficient and convenient silver‐catalyzed procedure was developed for the synthesis of various phosphoryl‐substituted benzimidazo2,1‐aisoquinoline‐6(5H)‐ones from diarylphosphine oxides and ...2‐arylbenzoimidazoles. Moreover, the phosphoryl‐substituted indolo2,1‐aisoquinolin‐6(5H)‐ones could also be achieved by using this strategy under mild reaction conditions in the presence of AgNO3/K2S2O8.
Phosphorylation cyclization of 2‐arylbenzoimidazoles and 2‐arylindoles derivatives was achieved by AgNO3/K2S2O8 catalytic system. Various phosphoryl‐substituted polycycels (benzimidazo2,1‐aisoquinoline‐6(5H)‐ones and indolo2,1‐aisoquinolin‐6(5H)‐ones) were synthesized by using this strategy under mild reaction conditions.
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FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SAZU, SBCE, SBMB, UL, UM, UPUK
We have generated an iPSCs line (CTGUi001-A) from dermal fibroblasts of a 16-year-old male Fabry disease patient with a novel GLA gene mutation (c.156C > A) using Sendai virus encoding the four ...Yamanaka factors OCT4, SOX2, KLF4, and c-MYC. The CTGUi001-A iPSC line displayed typical embryonic stem cell-like morphology, carried the GLA gene mutation, expressed several pluripotent stem cell makers, retained normal karyotype (46, XY) and was capable of forming teratomas containing three germ layers.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
We have generated PUMCi001-A, an induced pluripotent stem cells (iPSC) line from dermal fibroblasts of a 13-year-old male Krabbe disease patient with two hemizygous (461C > A and 1244G > A) mutations ...in Galactocerebrosidase (GALC) gene using a Sendai viral delivery of OCT4, SOX2, KLF4, and c-MYC. The PUMCi001-A iPSC line carried the GALC mutations, displayed typical iPSC morphology, expressed pluripotent stem cell makers, exhibited a normal karyotype and differentiation capacity into three germ layers.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
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