Plant cell cultures have emerged as a promising tool for producing active molecules due to their numerous advantages over traditional agricultural methods. Flavonols, and anthocyanin pigments in ...particular, together with other phenolic compounds such as chlorogenic acid, are known for their beneficial health properties, mainly due to their antioxidant, antimicrobial, and anti-inflammatory activities. The synthesis of these molecules is finely regulated in plant cells and controlled at the transcriptional level by specific MYB and bHLH transcription factors that coordinate the transcription of structural biosynthetic genes. The co-expression of peach
and
in tobacco was used to develop tobacco cell lines showing high expression of both the peach transgenes and the native flavonol structural genes. These cell lines were further selected for fast growth. High production levels of chlorogenic acid, anthocyanins (mainly cyanidin 3-rutinoside), and other phenolics were also achieved in pre-industrial scale-up trials. A single-column-based purification protocol was developed to produce a lyophile called ANT-CA, which was stable over time, showed beneficial effects on cell viability, and had antioxidant, anti-inflammatory, antibacterial, and wound-healing activities. This lyophile could be a valuable ingredient for food or cosmetic applications.
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IZUM, KILJ, NUK, PILJ, PNG, SAZU, UL, UM, UPUK
•Patients with MCL experiencing late relapse benefit from BTK-inhibitors over immunochemotherapies.•Overall, immunochemotherapies as second-line treatment are discouraged in the era of chimeric ...antigen receptor T-cell therapies.
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Patients with mantle cell lymphoma (MCL) who experience first relapse/refractoriness can be categorized into early or late progression-of-disease (POD) groups, with a threshold of 24 months from the initial MCL diagnosis. Bruton tyrosine kinase inhibitors (BTKi) are the established standard treatment at first relapse, but their effectiveness compared with chemoimmunotherapy (CIT) in late-POD patients remains unknown. In this international, observational cohort study, we evaluated outcomes among patients at first, late POD beyond 24 months. Patients treated upfront with BTKi were excluded. The primary objective was progression-free survival from the time of second-line therapy (PFS-2) of BTKi vs CIT. After accrual, all patients were prospectively followed up. Overall, 385 late-POD patients were included from 10 countries. Their median age was 59 years (range, 19-70), and 77% were male. Median follow-up from the time of first relapse was 53 months (range, 12-144). Overall, 114 patients had second-line BTKi, whereas 271 had CIT, consisting of rituximab-bendamustine (R-B; n = 101), R-B and cytarabine (R-BAC; n = 70), or other regimens (mostly cyclophosphamide-hydroxydaunorubicin-vincristine-prednisone- or platinum-based; n = 100). The 2 groups were balanced in clinicopathological features and median time to first relapse (48 months for both). Overall, BTKi was associated with significantly prolonged median PFS-2 than CIT (not reached NR vs 26 months, respectively; P = .0003) and overall survival (NR and 56 months, respectively; P = .03). Multivariate analyses showed that BTKi was associated with lower risk of death than R-B and other regimens (hazard ratio, 0.41 for R-B and 0.46 for others), but similar to that associated with R-BAC. These results may establish BTKi as the preferable second-line approach in patients with BTKi-naïve MCL.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Summary
Ursu Lake is located in the Middle Miocene salt deposit of Central Romania. It is stratified, and the water column has three distinct water masses: an upper freshwater‐to‐moderately saline ...stratum (0–3 m), an intermediate stratum exhibiting a steep halocline (3–3.5 m), and a lower hypersaline stratum (4 m and below) that is euxinic (i.e. anoxic and sulphidic). Recent studies have characterized the lake's microbial taxonomy and given rise to intriguing ecological questions. Here, we explore whether the communities are dynamic or stable in relation to taxonomic composition, geochemistry, biophysics, and ecophysiological functions during the annual cycle. We found: (i) seasonally fluctuating, light‐dependent communities in the upper layer (≥0.987–0.990 water‐activity), a stable but phylogenetically diverse population of heterotrophs in the hypersaline stratum (water activities down to 0.762) and a persistent plate of green sulphur bacteria that connects these two (0.958–0.956 water activity) at 3–3.5 to 4 m; (ii) communities that might be involved in carbon‐ and sulphur‐cycling between and within the lake's three main water masses; (iii) uncultured lineages including Acetothermia (OP1), Cloacimonetes (WWE1), Marinimicrobia (SAR406), Omnitrophicaeota (OP3), Parcubacteria (OD1) and other Candidate Phyla Radiation bacteria, and SR1 in the hypersaline stratum (likely involved in the anaerobic steps of carbon‐ and sulphur‐cycling); and (iv) that species richness and habitat stability are associated with high redox‐potentials. Ursu Lake has a unique and complex ecology, at the same time exhibiting dynamic fluctuations and stability, and can be used as a modern analogue for ancient euxinic water bodies and comparator system for other stratified hypersaline systems.
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BFBNIB, FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SBCE, SBMB, UL, UM, UPUK
We have identified EMS-induced mutations in
Drosophila Miro (dMiro), an atypical mitochondrial GTPase that is orthologous to human Miro (hMiro). Mutant
dmiro animals exhibit defects in locomotion and ...die prematurely. Mitochondria in
dmiro mutant muscles and neurons are abnormally distributed. Instead of being transported into axons and dendrites, mitochondria accumulate in parallel rows in neuronal somata. Mutant neuromuscular junctions (NMJs) lack presynaptic mitochondria, but neurotransmitter release and acute Ca
2+ buffering is only impaired during prolonged stimulation. Neuronal, but not muscular, expression of dMiro in
dmiro mutants restored viability, transport of mitochondria to NMJs, the structure of synaptic boutons, the organization of presynaptic microtubules, and the size of postsynaptic muscles. In addition, gain of dMiro function causes an abnormal accumulation of mitochondria in distal synaptic boutons of NMJs. Together, our findings suggest that dMiro is required for controlling anterograde transport of mitochondria and their proper distribution within nerve terminals.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
In this work, we demonstrate the two-photon fluorescence of covellite-phase copper sulfide nanodisks and investigate the role of the surface plasmon resonance on emission. Using selenium doping, we ...blue-shift the plasmon resonance toward the two-photon absorption edge. We observed a 3-fold enhancement of emission in these samples and report two-photon action cross sections that are an order of magnitude greater than conventional fluorophores. These nanomaterials offer a novel “all-in-one” platform for engineering plasmon–exciton coupling in the absence of a physical or chemical interface.
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IJS, KILJ, NUK, PNG, UL, UM
The COVID-19 pandemic and consequent physical distancing has made it difficult to provide care for those with Treatment-Resistant Depression (TRD). As a secondary analysis of a clinical trial, the ...aim of this study was to explore potential mechanisms through which three online-delivered approaches, added to treatment as usual, improve depressive symptoms in TRD patients.
The three approaches included (a) Minimal Lifestyle Intervention (MLI), (b) Mindfulness-Based Cognitive Therapy (MBCT), and (c) Lifestyle Modification Program (LMP). Sixty-six participants with TRD completed assessments pre-post intervention (mindfulness skills FFMQ; self-compassion SCS; and experiential avoidance AAQ-II) and pre-intervention to follow-up (depressive symptoms BDI-II). Data were analyzed using within-subjects regression models to test mediation.
Mindfulness skills mediated the effect of MBCT on depressive symptoms (
= -4.69, 95% CI = -12.93 to-0.32), whereas the lack of experiential avoidance mediated the effect of LMP on depressive symptoms (
= -3.22, 95% CI = -7.03 to-0.14).
Strengthening mindfulness skills and decreasing experiential avoidance may promote recovery in patients with TRD, MBCT, and LMP have demonstrated that they may help increase mindfulness skills and decrease experiential avoidance, respectively. Future work will need to unpick the components of these interventions to help isolate active ingredients and increase optimization.
A complex interplay between the crystal structure and the electron behavior within borophene renders this material an intriguing 2D system, with many of its electronic properties still undiscovered. ...Experimental insight into those properties is additionally hampered by the limited capabilities of the established synthesis methods, which, in turn, inhibits the realization of potential borophene applications. In this multimethod study, photoemission spectroscopies and scanning probe techniques complemented by theoretical calculations have been used to investigate the electronic characteristics of a high-coverage, single-layer borophene on the Ir(111) substrate. Our results show that the binding of borophene to Ir(111) exhibits pronounced one-dimensional modulation and transforms borophene into a nanograting. The scattering of photoelectrons from this structural grating gives rise to the replication of the electronic bands. In addition, the binding modulation is reflected in the chemical reactivity of borophene and gives rise to its inhomogeneous aging effect. Such aging is easily reset by dissolving boron atoms in iridium at high temperature, followed by their reassembly into a fresh atomically thin borophene mesh. Besides proving electron-grating capabilities of the boron monolayer, our data provide comprehensive insight into the electronic properties of epitaxial borophene which is vital for further examination of other boron systems of reduced dimensionality.
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IJS, KILJ, NUK, PNG, UL, UM
Memoria (licenciado en ciencias jurídicas y sociales)
En la parte inicial del primer capítulo de esta memoria nos abocaremos a estudiar entre otros temas su concepto, características, tipos, ...funciones y los cambios que ha experimentado en el último siglo. También señalaremos su naturaleza jurídica y la noción que de ella entrega nuestra Constitución Política. ;
La segunda parte del primer capítulo consistirá en retratar los fundamentos y aspectos históricos de la protección familiar por la Seguridad Social, para ello haremos un breve estudio de esta rama del Derecho, seguido del análisis de los fundamentos que hacen merecedora a la familia de resguardo por parte de la Seguridad Social. Finalmente concluiremos este primer capítulo con una descripción de la evolución histórica que ha tenido tanto nuestro Régimen de Seguridad Social como las más importantes prestaciones que éste ha otorgado a la familia.;
El segundo capítulo lo dedicaremos en su totalidad a tratar las diferentes herramientas de protección familiar existentes en el Derecho de la Seguridad Social. Para esto dividiremos nuestro estudio en 5 secciones, cada una de las cuales estará dedicada al estudio de las prestaciones que los diferentes regímenes existentes en nuestro Sistema de Seguridad Social conceden a la familia. Así en la primera y segunda sección analizaremos los aspectos más relevantes del Régimen de Asignación Familiar Regular y del Régimen de Subsidio Familiar Asistencial, respectivamente, en donde se examinarán sus conceptos, características, beneficiarios, formas de obtención y de pago, entre otros puntos. La tercera y cuarta sección se concentrarán en el estudio de las prestaciones que los Regímenes de Pensiones y de la Ley de Accidentes del Trabajo y Enfermedades Profesionales, respectivamente, entregan a la familia, constituidas principalmente por la denominada Pensión de Sobrevivencia. Finalmente, la quinta sección estará dedicada a los beneficios otorgados a la institución familiar por el Régimen Público de Salud.;
El tercer y último capítulo indagará en dos de la problemáticas más significativas que enfrenta la familia moderna en el Sistema de Seguridad Social actual. Nos referimos a la equidad de género en el Sistema Previsional y al tratamiento jurídico de las uniones de hecho y sus beneficios previsionales.
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