Chokeberries (
) are rarely used in diet in Croatia but they have high content of polyphenolic compounds and one of the highest
antioxidant activities among fruits. The aim of this study is to ...compare the quality, phenolic content and antioxidant capacity of different chokeberry products (juices, powders, fruit tea, capsules and dried berries). It can be expected that processing influences antioxidant activity and phenolic content of final products reaching consumers. Characterisation of phenolic compounds was carried out by using spectroscopic methods (Folin-Ciocalteu and pH differential methods). Antioxidant activity of chokeberry products was determined using 2,2-diphenyl-2-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) methods. The results show that the investigated products contain high amount of phenols (3002 to 6639 mg per L and 1494 to 5292 mg per 100 g of dry matter) and lower amount of total anthocyanins (150 to 1228 mg per L and 141 to 2468 mg per 100 g of dry matter). The examined juices and other chokeberry products possess high antioxidant capacity (12.09 to 40.19 mmol per L or 58.49 to 191.31 mmol per 100 g of dry matter, respectively) and reducing power (38.71 to 79.86 mmol per L or 13.50 to 68.60 mmol per 100 g of dry matter, respectively). On the basis of phenolic content and antioxidant activity, capsules and powders stand out among other products. The study indicates that there are significant differences (p<0.05) in the quality, phenolic content and antioxidant capacity among examined products.
: Total knee arthroplasty (TKA) has become the treatment of choice for advanced osteoarthritis. The aim of this paper was to show the possibilities of optimizing the Perth CT protocol, which is ...highly effective for preoperative planning and postoperative assessment of alignment.
: The cross-sectional study comprised 16 patients for preoperative planning or postoperative evaluation of TKA. All patients were examined with the standard and optimized Perth CT protocol using advance techniques, including automatic exposure control (AEC), iterative image reconstruction (IR), as well as a single-energy projection-based metal artifact reduction algorithm for eliminating prosthesis artifacts. The effective radiation dose (E) was determined based on the dose report. Imaging quality is determined according to subjective and objective (values of signal to noise ratio (SdNR) and figure of merit (FOM)) criteria.
: The effective radiation dose with the optimized protocol was significantly lower compared to the standard protocol (
< 0.001), while in patients with the knee prosthesis, E increased significantly less with the optimized protocol compared to the standard protocol. No significant difference was observed in the subjective evaluation of image quality between protocols (
> 0.05). Analyzing the objective criteria for image quality optimized protocols resulted in lower SdNR values and higher FOM values. No significant difference of image quality was determined using the SdNR and FOM as per the specified protocols and parts of extremities, and for the presence of prothesis.
: Retrospecting the ALARA ('As Low As Reasonably Achievable') principles, it is possible to optimize the Perth CT protocol by reducing the kV and mAs values and by changing the collimation and increasing the pitch factor. Advanced IR techniques were used in both protocols, and AEC was used in the optimized protocol. The effective dose of radiation can be reduced five times, and the image quality will be satisfactory.
The objective of this paper is to emphasize, branding as one of the possible ways to take concrete steps towards reaffirming the local values and traditional knowledge of rural populations in Serbia. ...Based on information gathered in the field, as well as from existing literature sources, the main goal of this study is to promote products from the Visok microregion. Due to very alarming demographic data, this may be the last chance for revitalizing this region. In this study, we highlighted the following products: Pirot kilim (Pirot rug), Pirot/Stara Planina lamb, Pirot Kachkaval cheese, Stara Planina honey, and Stara Planina potato. Besides agriculture, micr oregion Visok also has exceptional tourist potential. The conclusion of this paper emphasizes that the only viable path to revitalization is through planned investment. Such investment would facilitate the development of local infrastructure and help restore the population, thus enabling the creation of sustainable development.
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CEKLJ, NUK, ODKLJ, UL, UM, UPUK
Electronic nose (e-nose) and electronic tongue (e-tongue) instrumental systems were designed to crudely mimic human olfactory and taste sensory organs and are composed of an array of sensors. Complex ...data sets from electronic nose and electronic tongue signals combined with multivariate statistics represent rapid and efficient tools for classification, discrimination, recognition and identification of samples, as well as for the prediction of concentrations of different compounds. A wide variety of sensors can be employed into the design of these instrumental systems, especially that of electronic tongues, offering numerous practical applications. In this study, characteristics of sensors and possibilities of electronic nose and electronic tongue applications in the dairy industry were reviewed.
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IZUM, KILJ, NUK, PILJ, PNG, SAZU, UL, UM, UPUK
Multiculturalism policy in Serbia is an example of compromises made by monoculturalists between the issues surpassing the conservative paradigm of tolerance for ethnic and cul-tural differences and ...the normative protection of their identities. An unsystematised ap-proach to shaping multiculturalism policy led to disregard or misinterpretation of demo-graphic factors. Through the examples of how the rights to ethnic and cultural identities are obstructed for the Bosniak population in Priboj and Aromanians in Serbia, and the analysis of problems stemming from the centralist organisation of minority self-governments, the paper points to the weaknesses of the current multiculturalism policy and the need for introducing demographic criteria for it to be brought into line with the nature of multiethnicity in the country. The paper points to the issues and difficulties arising from this for the Roma national minority in the realisation of their rights. Despite of being a large national minority, its members are not able to enjoy full cultural autonomy because the realisation of rights is not in line with the demographic characteristics of the Roma. A reform of the multiculturalism policy would remove the existing obstacles and enable effective protection of ethnic, cultural and linguistic identities of minorities.
Isolated cell walls from maize (Zea mays L.) roots exhibited ionically and covalently bound NAD-specific malate dehydrogenase activity. The enzyme catalyses a rapid reduction of oxaloacetate and much ...slower oxidation of malate. The kinetic and regulatory properties of the cell wall enzyme solubilized with 1M NaCl were different from those published for soluble, mitochondrial or plasma membrane malate dehydrogenase with respect to their ATP, Pi, and pH dependence. Isoelectric focusing of ionically-bound proteins and specific staining for malate dehydrogenase revealed characteristic isoforms present in cell wall isolate, different from those present in plasma membranes and crude homogenate. Much greater activity of cell wall-associated malate dehydrogenase was detected in the intensively growing lateral roots compared to primary root with decreased growth rates. Presence of Zn2+ and Cu2+ in the assay medium inhibited the activity of the wall-associated malate dehydrogenase. Exposure of maize plants to excess concentrations of Zn2+ and Cu2+ in the hydroponic solution inhibited lateral root growth, decreased malate dehydrogenase activity and changed isoform profiles. The results presented show that cell wall malate dehydrogenase is truly a wall-bound enzyme, and not an artefact of cytoplasmic contamination, involved in the developmental processes, and detoxification of heavy metals.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPUK
As the loss of Kosovo has been de facto re-inflicted in recent years, the question should be raised as to how great an influence the Kosovo myth wields today. It is particularly interesting to ...consider the issue in the context of European integration and the fact that recognition of Kosovo and Metohija’s self-proclaimed independence is usually stated as an essential condition and a key obstacle to Serbia’s accession to the EU. It is an undisputable fact that Kosovo and Metohija and the Kosovo myth are a part of culture, history, religion, and folklore of Serbian people, and it is without doubt difficult to renounce all that, even if personal and collective prosperity is offered in lieu of it, which is deemed by a certain portion of the Serbian public to come as a result of the accession to the EU. The aim of this paper is to establish which portion of public opinion is willing to make such a sacrifice in relation to the current attempts at resolution of the Kosovo question. It is the authors’ ambition to answer the following question: To what extent does the position on the status of Kosovo and Metohija influence the public opinion on Serbia’s membership in the European Union? With an intention of answering the research question the authors rely on the data acquired through empirical-quantitative study The Notions of the EU and Russia in the Serbian Public 2018, Institute of Social Sciences Belgrade, where a structured on-line questionnaire was used as a means of data collection. The study results clearly indicate the popular support for Serbia’s accession to the EU while at the same time the method of resolution of the Kosovo question deeply impacts the public opinion on Serbia’s membership in the EU. A conclusion can be drawn that, if the public is presented with the choice between Kosovo and the EU, the certainty of citizens opting for the EU will rapidly decline.
Monitoring genetic uniformity and identity is very important in maize breeding and seed production. Due to practical reasons, molecular and biochemical methods for the determination of genetic purity ...and uniformity should reliably reflect genetic differences associated with traditional morphological traits, even when they are not agriculturally important. A comparison of genetic purity and uniformity of one maize hybrid lot, based on morphological UPOV markers and the UTLIEF (Ultrathin-layer isoelectric focusing) method is performed in this research. Tested hybrid samples expressed uniform protein markers profiles, but on the other hand, unsatisfactory uniformity of morphological markers in the field, indicating some laches in seed production. Although the UTLIEF method, standardized by ISTA rules, provides enough accurate distinction between F1 seed and self-pollinated maternal seed, a "clean" isozyme or protein profile will not necessarily correlate with morphological homogeneity. It is most likely that the non-uniformity of the tested hybrid lot originates from the non-uniformity of one of the parental lines. Therefore, to establish where the laches occurred, it is necessary to perform post-control tests with reference samples of maize hybrid and parental lines, as well as, the insight into their official descriptions according to the UPOV descriptor. Thus, it is very important to require the deposition of referent samples of hybrids and varieties whose seed production is allowed in Serbia, as well as to have harmonised protocols for conduction of laboratory and field post-control tests.
Total flavonoid and total phenolic content were studied in acacia and multifloral honey for 12 months in 6-month intervals. DPPH (1,1-diphenyl-2-picrylhydrazyl) and FRAP (ferric reducing antioxidant ...power) methods were used to determine total antioxidant activity in honey samples during the same period of time. Samples were stored in transparent glass containers at room temperature, on shelves exposed to natural light during daytime and in the dark during nighttime. Two types of honey from four different regions in Varazdin county, Croatia, were investigated: monofloral--acacia (Robinia pseudoacacia L.) and multifloral. Of the total of 40 samples, there were 20 of each type of honey (5 from each region). The goal of this study is primarily to demonstrate the changes in the antioxidant activity of the two investigated types of honey during one year of storage, and not to make comparisons between them. According to the obtained data, the rate of decrease in the content of total flavonoids and phenolics was determined and changes in the antioxidant activity in honey samples were measured. After one year of storage, total phenolic content decreased by 91.8 % in acacia honey, and by 88.6 % in multifloral honey. Total flavonoid content also decreased in both types of honey, by 45.6 % in acacia honey and by 43.8 % in multifloral honey. During the same period, an increase from 12.20 to 16.73 mg/mL (i.e. by 37.1 %) was recorded in the IC50 values in multifloral honey, while in acacia honey this increase was from 44.64 to 407.01 mg/mL (i.e. by 811.7 %). Decrease in the antioxidant activity measured by FRAP method was also bigger in acacia honey than in multifloral honey (by 428.0 and 72.5 %, respectively), which corresponds well with the results obtained by DPPH method. Simple correlations were made to determine how each of the investigated parameters affects the others. The analysis of variance was used in order to determine the influence of the region, honey type and storage time on different parameters of antioxidant activity as well as on the total phenolic and total flavonoid content in honey samples. Key words: antioxidant activity, phenolic compounds, honey, storage
Information regarding the adaptation of maize (Zea mays L .) inbred lines to various field conditions and seed longevity is essential for seed companies. The objective of this work was to evaluate ...different vigour tests for detecting differences among naturally aged seeds and to assess the relationship between seed quality parameters and pedigree data of maize inbred lines. A seed of 15 maize inbred lines, selected at Maize Research Institute Zemun Polje, belonging to different heterotic groups was produced during 2011. Seed quality was assessed in 2015 by standard germination , radical emergence, accelerated ageing, and cold test. Root length of 4-day old seedlings was measured in all applied tests. Analysis of variance showed that genotype and storage conditions had a significant effect on all seed quality parameters. Large variation among inbred lines was detected in seed vigour determined by three tests, in particular by the accelerated ageing test. Cold test results showed very good chilling tolerance of tested genotypes, except two inbred lines with Lancaster background. The correlation between the cold test and the accelerated ageing test was significant, while the radical emergence test had a poor correlation with them. Obtained results indicate that the application of either cold test or accelerated ageing test is reliable for screening seed vigour of maize inbred lines.
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