Aim
Recent studies point at the crucial role of epigenetic mechanisms in the development of multifactorial diseases such as periodontitis and diabetes mellitus (DM) type 2. In addition, circulatory ...microRNAs (miRs) have emerged as novel biomarkers for various diseases. Aim of this study was to investigate the levels of miR‐146a and miR‐155 and superoxide dismutase (SOD) activity in gingival crevicular fluid (GCF) of periodontitis patients with (CPDM) and without (CP) DM type 2 as well as in periodontally healthy, control groups (PHDM and PH, respectively).
Material and methods
miR modulation was analysed using quantitative real‐time PCR while SOD activity was measured spectrophotometrically.
Results
The upregulation of miR‐146a and miR‐155 was observed in CP and CPDM patients’ baseline, while the levels decreased after 6 weeks of the non‐surgical therapy to the levels comparable to PH and PHDM, respectively. Expression levels of miRs positively correlated with SOD activity. Levels of miR‐146a were higher in PHDM compared to PH patients. Multivariate analysis revealed that levels of miR‐146a and miR‐155 were significantly associated with periodontitis when adjusting for age and gender.
Conclusions
miR‐146a and miR‐155 may be considered as possible novel biomarkers for periodontitis in non‐diabetic and type 2 diabetic patients.
Full text
Available for:
BFBNIB, CMK, FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SAZU, SBCE, SBMB, UL, UM, UPUK
It is a standard view that concessive clauses (concessives) cross-linguistically cannot be used in the scope of focus particles such as only, even, just, unlike the majority of other adverbial ...clauses (e.g. König 2006; Mizuno 2007). It is usually assumed that this is due to their lesser degree of integration with the main clause compared to other adverbial clauses (König 2006). We argue for an alternative view: standard concessives (concessives marking the standard concessive relation, cf. König 2006) are inherently focused, i.e. they compositionally include a (c)overt focus particle responsible for the scalar ranking of the asserted and presupposed propositions. We build on Lund’s (2017) decompositional analysis of even though in English to provide theoretical and quantitative evidence from Serbian that standard concessives include a focus particle, which bans their combination with other focus particles, due to complementary distribution. Specifically, we analyze three main concessive conjunctions in Serbian – iako, mada and premda (traditionally considered fully synonymous), addressing the question of why only iako is compatible with the focus particle čak ‘even’. We argue that this is a consequence of čak being an integral part of constructions with standard iako-concessives. We show that properties of concessives introduced by the three concessive conjunctions under discussion correlate with the meaning of (c)overt focus particles contained in them. We also sketch some consequences for the syntax of concessives.
In this study, we demonstrate for the first time, that a discrete metal-oxo cluster α-/β-K
P
W
O
(WD-POM) exhibits superior performance as a computed tomography (CT) contrast agent, in comparison to ...the standard contrast agent iohexol. A toxicity evaluation of WD-POM was performed according to standard toxicological protocols using Wistar albino rats. The maximum tolerable dose (MTD) of 2000 mg/kg was initially determined after oral WD-POM application. The acute intravenous toxicity of single WD-POM doses (1/3, 1/5, and 1/10 MTD), which are at least fifty times higher than the typically used dose (0.015 mmol W kg
) of tungsten-based contrast agents, was evaluated for 14 days. The results of arterial blood gas analysis, CO-oximetry status, electrolyte and lactate levels for 1/10 MTD group (80% survival rate) indicated the mixed respiratory and metabolic acidosis. The highest deposition of WD-POM (0.6 ppm tungsten) was found in the kidney, followed by liver (0.15 ppm tungsten), for which the histological analysis revealed morphological irregularities, although the renal function parameters (creatinine and BUN levels) were within the physiological range. This study is the first and important step in evaluating side effects of polyoxometalate nanoclusters, which in recent years have shown a large potential as therapeutics and contrast agents.
Full text
Available for:
IZUM, KILJ, NUK, PILJ, PNG, SAZU, UL, UM, UPUK
High elution and diffusion of 2-hydroxylethyl methacrylate (HEMA) and camphorquinone (CQ) through dentinal tubules may induce pulp injury and postoperative sensitivity. We aimed to investigate the ...melatonin protective effect in HEMA- and CQ-treated human dental pulp cells (hDPCs) as well as its relevance in a mechanism for postoperative sensitivity in diabetic patients. hDPCs were exposed to HEMA (5 mM) and/or CQ (1 mM) in the absence and presence of melatonin (MEL) (0.1 mM and 1 mM). Heme oxygenase-1 (HMOX1), NADPH oxidase-4 (NOX4), BCL-2-associated X-protein (BAX), B-cell lymphoma-2 (BCL-2) and caspase-3 (CASP3) gene expression levels, and superoxide dismutase (SOD) activity were measured in hDPCs while inducible nitric oxide synthase (iNOS) and melatonin protein expression were measured in human dental pulp as well, by RT-PCR, by ELISA, and spectrophotometrically. Bioinformatic analyses were performed by using the ShinyGO (v.0.75) application. Type 2 diabetic patients showed a higher incidence of postoperative sensitivity and lower melatonin and higher iNOS content in dental pulp tissue compared with non-diabetic patients. Melatonin, when co-added in hDPC culture, reverses HEMA and CQ cytotoxic effects via anti-apoptotic and anti-inflammatory/antioxidant iNOS-related effects. Enrichment analyses showed that genes/proteins, altered by HEMA and CQ and normalized by melatonin, are the most prominently overrepresented in type 2 diabetes mellitus pathways and that they share subcellular localization in different oligomeric protein complexes consisting of anti- and pro-apoptotic regulators. This is the first evidence of the ability of melatonin to counteract iNOS-mediated inflammatory and stress effects in HEMA- and CQ-treated hDPCs, which could be of significance for the modulation of presently observed immediate postoperative sensitivity after composite restoration in type 2 diabetic patients.
Full text
Available for:
IZUM, KILJ, NUK, PILJ, PNG, SAZU, UL, UM, UPUK
Polyoxotungstate nanoclusters have recently emerged as promising contrast agents for computed tomography (CT). In order to evaluate their clinical potential, in this study, we evaluated the in vitro ...CT imaging properties, potential toxic effects in vivo, and tissue distribution of monolacunary Wells-Dawson polyoxometalate, α
-K
P
W
O
20H
O (mono-WD POM). Mono-WD POM showed superior X-ray attenuation compared to other tungsten-containing nanoclusters (its parent WD-POM and Keggin POM) and the standard iodine-based contrast agent (iohexol). The calculated X-ray attenuation linear slope for mono-WD POM was significantly higher compared to parent WD-POM, Keggin POM, and iohexol (5.97 ± 0.14 vs. 4.84 ± 0.05, 4.55 ± 0.16, and 4.30 ± 0.09, respectively). Acute oral (maximum-administered dose (MAD) = 960 mg/kg) and intravenous administration (1/10, 1/5, and 1/3 MAD) of mono-WD POM did not induce unexpected changes in rats' general habits or mortality. Results of blood gas analysis, CO-oximetry status, and the levels of electrolytes, glucose, lactate, creatinine, and BUN demonstrated a dose-dependent tendency 14 days after intravenous administration of mono-WD POM. The most significant differences compared to the control were observed for 1/3 MAD, being approximately seventy times higher than the typically used dose (0.015 mmol W/kg) of tungsten-based contrast agents. The highest tungsten deposition was found in the kidney (1/3 MAD-0.67 ± 0.12; 1/5 MAD-0.59 ± 0.07; 1/10 MAD-0.54 ± 0.05), which corresponded to detected morphological irregularities, electrolyte imbalance, and increased BUN levels.
Full text
Available for:
IZUM, KILJ, NUK, PILJ, PNG, SAZU, UL, UM, UPUK
6.
The importance of design of experiments Milosavljević, Aleksandra; Pavlov-Kagadejev, Marijana; Stolić, Predrag
Mining and Metallurgy Engineering Bor,
2024, Volume:
2024, Issue:
1
Journal Article
Peer reviewed
Open access
Design of experiments (DOE) is very meaningful and applied in various investigations from science to the industry in order to optimize the process itself. There are several such techniques and each ...of them has its own advantages, so it is very important to know the basics of DOE. Besides that, every problem, technology, product etc. is unique, so the knowledge about those is crucial as the first step. The most relevant fact is the dependence among variables-input factors and output responses as well as mutual connection between factors. In order to demonstrate the usability and adaptability of DOE for various purposes, some examples are given in this paper.
The aim of this study was to quantify the distribution of microvessels and mast cells in all three parts of the trigeminal ganglion (TG). Statistical analyses were applied to investigate possible ...micromorphological regional differences in their density. Five serially sectioned human TGs were prepared for CD34 and mast cell tryptase immunostaining. The following quantifications were performed in microscopic fields of three parts of the TG: microvessel density (MVD), mast cell density (MCD) and ganglionic cell count. The density of CD34-positive microvessels was not significantly different in any of the three observed parts of the TG. The distribution of neurons showed no significant statistical difference in three parts of the TG. There was no difference in the density of tryptase-positive mast cells within the TG, but there was an abundant presence of mast cells in the periganglionic dural and subdural tissues, a finding hitherto not reported. We can say that there is a homogenous vascular pattern within the TG which excludes local predominance in pathogenesis of trigeminal neuralgia. Second, and more important, the finding of peri-trigeminal mast cells indicates their important role in migraine pain and confirms their degranulation as the main therapeutic goal for this condition.
New global tendencies for waste materials reusing in building materials are
imposing the request for improved performances of chemical analysis methods
and the improvements of matrices used. A new ...method for optical emission
spectrometry with inductively coupled plasma (ICP-OES) is developed and
validated for the chemical analysis (35 elements: Al, Be, Cd, So, Cr, Cu,
Fe, Mn, Mo, Ni, V, Mo, Zn, Pb , Bi, Si, Zr, W, As, Se, Sb, Sn, Ti, Ba, B,
Ag, Mg, Ca, K, Na, S, P, Ga, In, Li) in leachate of fly ash. Validation
performances and the uncertainty ?f measurement were resolved. Uncertainty
?f measurements were resolved by three routes: validation procedure,
participation in proficiency testing (PT) schemes and standard method. The
obtained method is a new simple and effective analyzing route for
determination of undesired trace elements and their quantity comprised in
leachates of fly ash, and leachates of building materials with addition of
fly ash (cement binders and mortars). In order to prove its accuracy and
precision, the developed method was employed on laboratory samples of cement
binders and mortars. Results were compared with limit values provided in the
standard. Multivariate analyses, i.e. cluster analysis and principal
component analysis, were applied to establish interrelations between
analyzed samples, and to certify the developed ICP-OES method.
The Institute for Standardization of Serbia has adopted a large number of en standards in the field of bridges as Serbian standards. Unfortunately, technical regulations based on the implementation ...of the adopted en standards in the field of bridges are still not made. The paper presents requirements for railway bridges based on en standards, UIC leaflets and German technical regulations. The aspect of track/bridge interaction is primarily considered. The parameters of track/bridge interaction, principles of calculation, as well as the overview of open points are presented. As the most important parameters of the bridge structure were considered: bridge expansion length, stiffness of the bridge substructure, as well as the bending stiffness and height of the bridge deck. Further, the effects of vertical load, temperature changes, and acceleration/breaking of the vehicle on the mentioned interaction are especially analysed. In addition, the conclusions of the paper apply to both ballasted track and slab track. The aim of the paper is to create a basis for the harmonisation of technical regulations in Serbia with European regulations in order to meet the requirements of interoperability.
Copper-based shape memory alloy with zinc and aluminum was manufactured, plastically deformed, heat treated and characterized in terms of the physico-mechanical, structural and micro-structural ...investigation. Typical martensitic microstructure with twins is revealed by the optical and electron microscopy. The presence of marten site in the structure was further confirmed through an X-ray diffraction. Toughness and hardness of the alloy are investigated, too. Optimal properties are obtained for the condition of the alloy that was subjected to the heat treatment according to the following scheme: annealing at 8500C and 9000C (10 min) + quenched in water + aging at 4000C (1 hour) + air cooling.
PDF
