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•Thirty PC specimen made with FDM were tested contributing to data availability.•Correlation of physical test and Finite Element Analysis has been performed.•FEA isotropic model may ...be better than orthotropic model for elastic region.•Manufacturing parameters affect mechanical response besides building orientation.
Building end-use functional parts with additive manufacturing (AM) technologies is a challenging task. Several factors influence their surface finish, dimensional accuracy, mechanical properties and cost. Their orientation inside the building chamber is one of the most significant factors in AM processes. When using Fused Deposition Modeling (FDM) to build such parts, additional factors must be considered.
This paper aims to accomplish two purposes: finding a good model to simulate FDM parts and correlating a finite element analysis (FEA) simulation with physical testing.
The first objective was achieved by experimental tensile test of specimens to determine the nine mechanical constants that defines the stiffness matrix of an orthotropic material. Three Young’s modulus, three Poisson’s ratio and three shear modulus were experimentally obtained as well as yield tensile and ultimate strength of each specimen.
A simple part was designed and manufactured in different orientations to be physically tested and simulated to achieve the second objective. Polycarbonate (PC) was used as part material. Combined loading including bending and torsion was used. Differences on mechanical response were observed during the physical test of the parts depending on the building direction. Conclusions comment results and the convenience of using a different constitutive model depending on the design and use specifications.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK
Due to the anatomical continuity of the uterine cavity with the cervix, genomic exploitation of material from routine Pap smears and other noninvasive sampling methods represent a unique opportunity ...to detect signs of disease using biological material shed from the upper genital tract. Recent research findings offer a promising perspective in the detection of endometrial cancer, but certain questions need to be addressed in order to accelerate the implementation of novel technologies in a routine screening or clinical setting. We discuss here new perspectives on detection of endometrial cancer using genomic and other biomarkers in minimally invasive sampling methods with a special focus on public health classic screening criteria, highlighting current gaps in knowledge.
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BFBNIB, FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SBCE, SBMB, UL, UM, UPUK
The conformational flexibility of the expanded porphyrins allows them to achieve different topologies with distinct aromaticities and nonlinear optical properties (NLOP). For instance, it is possible ...to switch between Möbius and Hückel topologies applying only small changes in the external conditions or in the structure of the ring. In this work, we evaluate the electronic and vibrational contributions to static and dynamic NLOP of the Hückel and Möbius conformers of A,D-di-p-benzi28hexaphyrin(1.1.1.1.1.1) synthesized by Latos-Grażyński and co-workers Angew. Chem., Int. Ed. 46, 7869 (2007). Calculations are performed at the HF, M052X, and CAM-B3LYP levels using the 6-31G, 6-311G(d), and 6-31+G(d) basis sets. Our results conclude that M052X∕6-31G and CAM-B3LYP/6-31G methods provide a correct qualitative description of the electronic and vibrational contributions for the NLOP of expanded porphyrins. The studied systems show high NLOP with large differences between the Möbius and Hückel conformations (around 1 × 10(6) a.u. for γ). The obtained results indicate that the expanded porphyrins are promising systems to manufacture Hückel-to-Möbius topological switches.
Induced pluripotent stem cells (iPSC) offer an unprecedented opportunity to model human disease in relevant cell types, but it is unclear whether they could successfully model age‐related diseases ...such as Parkinson's disease (PD). Here, we generated iPSC lines from seven patients with idiopathic PD (ID‐PD), four patients with familial PD associated to the G2019S mutation in the Leucine‐Rich Repeat Kinase 2 (LRRK2) gene (LRRK2‐PD) and four age‐ and sex‐matched healthy individuals (Ctrl). Over long‐time culture, dopaminergic neurons (DAn) differentiated from either ID‐PD‐ or LRRK2‐PD‐iPSC showed morphological alterations, including reduced numbers of neurites and neurite arborization, as well as accumulation of autophagic vacuoles, which were not evident in DAn differentiated from Ctrl‐iPSC. Further induction of autophagy and/or inhibition of lysosomal proteolysis greatly exacerbated the DAn morphological alterations, indicating autophagic compromise in DAn from ID‐PD‐ and LRRK2‐PD‐iPSC, which we demonstrate occurs at the level of autophagosome clearance. Our study provides an iPSC‐based in vitro model that captures the patients' genetic complexity and allows investigation of the pathogenesis of both sporadic and familial PD cases in a disease‐relevant cell type.
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FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SBCE, SBMB, UL, UM, UPUK
Recently, much attention has been focused on the design and synthesis of molecules with aromatic Möbius topology. One of the most promising applications is the manufacture of Hückel-to-Möbius ...topological optical switches with high nonlinear optical properties. In this work, we evaluate the electronic and vibrational contributions to static and dynamic nonlinear optical properties of the CS Hückel and C2 Möbius topologies synthesized by Herges and co-workers (Ajami, D. et al. Nature2003, 426, 819). Calculations are performed at the HF, B3LYP, BHandHLYP, BMK, M052X, CAM-B3LYP, and MP2 levels with the 6-31+G(d) basis set. Our results conclude that the BHandHLYP, M052X, and CAM-B3LYP methods correctly reproduce the X-ray crystal structure and provide similar nonlinear optical properties.
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IJS, KILJ, NUK, PNG, UL, UM
Thin, condensed coarse‐grained shallow marine successions can be difficult to describe and interpret, especially in the subsurface since the recognition of finer‐grained intervals, typically ...associated with sequence stratigraphic surfaces, is challenging. This lack of mudstones and siltstones means that they also typically make excellent reservoir intervals. The Oxfordian to Volgian intra‐Draupne Formation sandstones in the Johan Sverdrup Field, southern Utsira High, represent such a system. This study presents a new sequence stratigraphic model for the Johan Sverdrup Field that unravels the detailed depositional history of the succession and places its formation within a regional Late Jurassic tectonostratigraphic framework. The intra‐Draupne Formation sandstones comprise four parasequences deposited following a regional Kimmeridgian marine flooding event. Sediments were mainly supplied through West‐derived fan deltas from the Haugaland High and NW‐SE‐directed tidal currents reworking the Augvald Graben and the Avaldsnes High at the East. The oldest parasequence shows a distinctive suite of facies consisting of fine‐grained and mud‐rich bioturbated sandstones deposited in a semi‐restricted lagoon. Subsequent parasequences lack fine‐grained sediments and are dominated by bidirectional cross‐stratified, very coarse‐to coarse‐grained sandstones and gravels deposited in a tidal strait. A progressive reduction of fault‐related subsidence in the Middle Volgian along with Late Volgian‐Ryazanian sea‐level rise and inversion of pre‐existing structures promoted backstepping of the feeder systems, sediment starvation and the progressive deposition of the black and green‐red shales of the Draupne and Asgard formations. The results of this study account for features previously unidentified in the Johan Sverdrup Field and which have implications for understanding the deposition of coarse‐grained shallow marine successions around the Utsira High and other transgressed basement highs.
Evolution of the different Late Jurassic‐Early Cretaceous depositional environments identified in the Johan Sverdrup Field.
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FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SBCE, SBMB, UL, UM, UPUK
The Catalan Cancer Plan (CCP) undertakes periodic audits of cancer treatment outcomes, including organ/space surgical site infections (O/S-SSI) rates, while the Catalan Healthcare-associated ...Infections Surveillance Programme (VINCat) carries out standardized prospective surveillance of surgical site infections (SSI) in colorectal surgery. This cohort study aimed to assess the concordance between these two monitoring systems for O/S-SSI following primary rectal cancer surgery.
The study compared O/S-SSI incidence data from CCP clinical audits versus the VINCat Programme in patients undergoing surgery for primary rectal cancer, in 2011-12 and 2015-16, in publicly funded centres in Spain. The main outcome variable was the incidence of O/S-SSI in the first 30 days after surgery. Concordance between the two registers was analysed using Cohen's kappa. Discordant cases were reviewed by an expert, and the main reasons for discrepancies evaluated.
Pooling data from both databases generated a sample of 2867 patients. Of these, O/S-SSI was detected in 414 patients-235 were common to both registry systems, with satisfactory concordance (κ = 0.69, 95% confidence interval 0.65-0.73). The rate of discordance from the CCP (positive cases in VINCat and negative in CCP) was 2.7%, and from VINCat (positive in CCP and negative in VINCat) was 3.6%. External review confirmed O/S-SSI in 66.2% of the cases in the CCP registry and 52.9% in VINCat.
This type of synergy shows the potential of pooling data from two different information sources with a satisfactory level of agreement as a means to improving O/S-SSI detection.
gov Identifier: NCT06104579. Registered 30 November 2023.
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IZUM, KILJ, NUK, PILJ, PNG, SAZU, UL, UM, UPUK
Screening for colorectal cancer (CRC) is effective in the population at average risk. The most extended strategy in organized programs involves the fecal immunochemical test, which is limited by low ...sensitivity for the detection of advanced adenomas (AAs). We aimed to identify microRNA (miRNA) signatures in fecal samples that identify patients with AAs or CRC and might be used in noninvasive screening.
Our study comprised 4 stages. In the discovery phase, we performed genome-wide miRNA expression profiling of 124 fresh, paired colorectal tumor and nontumor samples (30 CRC; 32 AAs) from patients in Spain. In the technical validation stage, miRNAs with altered expression levels in tumor vs nontumor tissues were quantified by reverse-transcription polymerase chain reaction in fecal samples from a subset of patients included in the discovery phase (n = 39) and individuals without colorectal neoplasms (controls, n = 39). In the clinical validation stage, the miRNAs found to be most significantly up-regulated by quantitative reverse transcription polymerase chain reaction analysis were measured in an independent set of fecal samples (n = 767) from patients with positive results from fecal immunochemical tests in a CRC screening program. Finally, we developed a model to identify patients with advanced neoplasms (CRCs or AAs) based on their miRNA profiles, using findings from colonoscopy as the reference standard.
Among 200 and 324 miRNAs significantly deregulated in CRC and AA tissues, respectively, 7 and 5 of these miRNAs were also found to be deregulated in feces (technical validation). Of them, MIR421, MIR130b-3p, and MIR27a-3p were confirmed to be upregulated in fecal samples from patients with advanced neoplasms. In our model, the combination of fecal level of MIR421, MIR27a-3p, and hemoglobin identified patients with CRC with an area under the curve (AUC) of 0.93, compared with an AUC of 0.67 for fecal hemoglobin concentration alone.
We found that increased levels of 2 miRNAs and hemoglobin in feces can identify patients with AAs or CRC more accurately than fecal hemoglobin concentration alone. Assays for these miRNAs might be added to fecal tests for the detection of CRC or AAs.
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