Abstract
The major histocompatibility complex (
MHC
) is most closely associated with nasopharyngeal carcinoma (
NPC
), but the complexity of its genome structure has proven challenging for the ...discovery of causal
MHC
loci or genes. We conducted a targeted
MHC
sequencing in 40 Cantonese
NPC
patients followed by a two‐stage replication in 1065
NPC
cases and 2137 controls of Southern Chinese descendent. Quantitative
RT
‐
PCR
analysis (
qRT
‐
PCR
) was used to detect gene expression status in 108
NPC
and 43 noncancerous nasopharyngeal (
NP
) samples. Luciferase reporter assay and chromatin immunoprecipitation (Ch
IP
) were used to assess the transcription factor binding site. We discovered that a novel
SNP
rs117565607_A at
TRIM
26
displayed the strongest association (
OR
= 1.909, Pcombined = 2.750 × 10
−19
). We also observed that
TRIM
26
was significantly downregulated in
NPC
tissue samples with genotype
AA
/
AT
than
TT
. Immunohistochemistry (
IHC
) test also found the
TRIM
26
protein expression in
NPC
tissue samples with the genotype
AA
/
AT
was lower than
TT
. According to computational prediction, rs117565607 locus was a binding site for the transcription factor Yin Yang 1 (
YY
1). We observed that the luciferase activity of
YY
1 which is binding to the A allele of rs117565607 was suppressed. Ch
IP
data showed that
YY
1 was binding with T not A allele. Significance analysis of microarray suggested that
TRIM
26
downregulation was related to low immune response in
NPC
. We have identified a novel gene
TRIM
26
and a novel
SNP
rs117565607_A associated with
NPC
risk by regulating transcriptional process and established a new functional link between
TRIM
26
downregulation and low immune response in
NPC
.
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FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SBCE, SBMB, UL, UM, UPUK
AIM： TO evaluate the safety, efficacy and management of double balloon enteroscopy （DBE） carried out in those aged individuals with suspicious small intestine diseases. METHODS： DBE is a wonderful ...invention of the past decade and is widely used as an examination tool for the gastrointestinal tract. From January 2003 to July 2011, data from patients who were ≥ 65 years old and underwent DBE examination in the Nanfang Hospital were included in a retrospective analysis. RESULTS： Fifty-nine individuals were found and sub-sequently analyzed. The mean age was 69.63 ＋ 3.89 years （range 65-84）, 34 were males. Indications for DBE were melena/hematochezia （36 cases）, abdominal pain （15 cases）, diarrhea （3 cases）, stool change （1 case）, weight loss （1 case）, vomiting （2 cases）, and de- bilitation （1 case）. The average duration of symptoms was 33.34 ＋ 64.24 mo. Twenty-seven patients suffered from age-related diseases. Severe complications were not found during and after DBE. Comparison between systolic and diastolic blood pressure before and after DBE was statistically significant （mean ＋ SD, P 〈 0.01, P 〈 0.05, respectively）. Small bowel pathologies were found by DBE in 35 patients, definite diagnoses were made in 31 cases, and detection rate and diagnostic yield for DBE were 68.6% and 60.8%, respectively. CONCLUSION： DBE is a safe and effective method for gastrointestinal examination in the aged popula- tion. Aging alone is not a risk factor for elderly patients with suspicious gastrointestinal diseases and thorough preparation prior to the DBE procedure should be made for individuals with multiple diseases especially cardio- pulmonary disorders.
Background
Asthmatic patients with chest tightness as their only presenting symptom (chest tightness variant asthma CTVA) have clinical characteristics of eosinophilic airway inflammation similar to ...those of classic asthma (CA); however, whether CTVA has similar response to antiasthma treatment as compared with CA remains unclear.
Objective
The response of 76 CTVA patients to standard asthma treatments with inhaled corticosteroids with long‐acting beta‐agonists was explored in a 52‐week multicenter, prospective, real‐world study.
Results
After 52 weeks of treatment with therapy regimens used for CA, the mean 5‐point Asthma Control Questionnaire (ACQ‐5) score decreased markedly from 1.38(first administration) to 0.71 (52 weeks, mean decrease: 0.674, 95%CI: 0.447‐0.900, P<.001).The mean asthma quality‐of‐life questionnaire (AQLQ) score increased from 5.77 (first administration) to 6.20 (52 weeks, mean increase: 0.441, 95% CI 0.258‐0.625, P<.001). Furthermore, at week 52, FVC, FEV1%, the diurnal variation in PEFand the PD20‐FEV1 were significantly improved. Subgroup analysis revealed that the patients at first administration in the responsive group had higher ACQ‐5 scores than those in the nonresponsive group (P < .05).
Conclusion
In conclusion, patients with CTVA had a good therapeutic response to the guideline‐recommended routine treatment (containing inhaled corticosteroids). The association between the treatment response and the severity of CTVA suggested that CTVA patients with higher ACQ‐5 scores had better therapeutic effects.
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FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SBCE, SBMB, UL, UM, UPUK
To evaluate the expression of BRCA1, ERCC1, TUBB3 and PRR13 mRNA and their relationship with clinical chemosensitivity in primary ovarian cancer, and to assess the predictive value of joint detection ...of both BRCA1 and ERCC1 genes for the treatment of primary ovarian cancer.
Primary epithelial ovarian tumor samples were collected from 46 patients who underwent cytoreductive surgery. Real-time quantitative PCR was used to analyze the relative expression of BRCA1, ERCC1, TUBB3 and PRR13 mRNA in those cases. The correlation of clinical chemosensitivity and the test results was statistically analyzed. The efficacy of the joint prediction of clinical chemosensitivity by combining the two drug resistance gene detection was evaluated.
The BRCA1 mRNA relative expression logarithm in the clinical-resistant group was 0.673±2.143, and clinical-sensitive group -1.436±2.594 (P=0.008). The ERCC1 mRNA relative expression logarithm in the clinical-resistant group was -0.529±1.982 and clinical-sensitive group -3.188±2.601 (P=0
To investigate the efficacy of adenosine triphosphate (ATP)-tumor chemosensitivity assay (TCA) directed chemotherapy in patients with recurrent epithelial ovarian cancer.
From August 2010 to June ...2012, recurrent epithelial ovarian cancer patients were prospectively enrollmented in Cancer Hospital, Peking Union Medical College,Chinese Academy of Medical Sciences.The entry criteria are as follows: (1) Histologically proven to be epithelial ovarian cancer. (2) Patients of recurrent ovarian cancer with bidimensionally measurable tumor, or ascitic or pleural fluid for testing. (3) Karnofsky performance status > 60. (4) A life expectancy of at least more than 6 months.According to patients desires, they were assigned into two groups: assay-directed therapy group and physician's-choice therapy group, patients' clinical and pathological characteristics, response rate to chemotherapy and progression-free survival (PFS) were compared between two groups.
A total of 113 patients with recurrent epithelial ovarian cancer w
Atomic bonding of the GPB zone and S″ phase of Al–Cu–Mg alloys in early aging stage are calculated using the empirical electron theory (EET) in solid. The results show that not only the covalence ...bond-network is very strong in GPB zone, but the whole covalence bond energy of S″ phase is also very large, and all the primary bond-net framework of these precipitates can consolidate the matrix of alloy. Phase transformation from GPB zone to S″ phase is explained reasonably based on atomic bonding and total binding capacity of Al and Cu atoms in these precipitates.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK
To predict clinical chemotherapy sensitivity of primary ovarian cancer by jointing adenosine triphosphate (ATP)-tumor chemo-sensitivity assay (TCA) method in vitro and detection of drug resistance ...genes, provide reference for clinical treatment.
Forty-seven primary epithelial ovarian tumor samples were collected from the patients who received cytoreductive surgery. Viable ovarian cancer cells obtained from malignant tissue were tested for their sensitivity to carboplatin (CBP), cisplatin (DDP), paclitaxel (PTX) and CBP + PTX using ATP-TCA method in vitro; at same time, real-time quantitative PCR was used to analysis BRCA1 and ERCC1 mRNA relative expression in forty-six specimens (1 frozen tumor samples mRNA were not detected due to serious degradation). The relationship between ATP-TCA test results, clinical indicators, and the effectiveness of the joint prediction on clinical chemo-sensitivity by combining these two methods were statistically analyzed using chi-square test.
(1) The results showns that three
To evaluate the predictive value of the adenosine triphosphate-tumor chemosensitivity assay (ATP-TCA) in the chemotherapy applied in primary epithelial ovarian cancer (PEOC), and to analyze if the ...neoadjuvant chemotherapy have any influence on the postoperative chemosensitivity.
ATP-TCA results from 61 PEOC specimens were analyzed retrospectively. Patients were divided into sensitive group and resistant group according to the ATP-TCA results. Sensitive index (SI) was applied to analyze the ATP-TCA results. The correlation between in vitro results and clinical outcome was assessed by univariate and multivariate analysis.
SI set at > 250 had the highest test sensitivity, specificity, positive and negative predictive value of 91.6%, 73.9%, 84.6% and 85.0%, respectively. The ATP-TCA results had significant correlation with clinical outcome (chi(2) = 26.9, P < 0.001). Patients with tumors shown to be resistant had a higher risk of recurrence in comparison with those who were tested as sensitive (P = 0.030, OR = 0.
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