•A novel VGMS made up of recycled/natural and highly performing materials from the energy/environmental point of view, has been designed, prototyped and in lab/in field monitored through a ...multidisciplinary approach.•The technological issues, biometric parameters, and the acoustic, thermal and mechanical aspects were complimentarily investigated.•Thermal performance analyses showed interesting effect both during heating and cooling seasons.•An acoustic analysis demonstrated that the system acts well as a sound insulation system, and its high sound absorption could be exploited to reduce the urban canyoning effect.
Vegetation in architecture can be considered a proper design strategy that is aimed at improving not only the performances of buildings, but also the outdoor climate. Different technological solutions have been proposed over the years to cover buildings with vegetation, i.e. green roofs, green walls and green balconies. A particular typology of green wall, which has recently been gaining high consensus among designers, is the vertical greenery modular system (VGMS). The positive impact of this type of technology on the performance of buildings is related to several factors, such as the façade orientation, the use of the building, climatic conditions, the type of plants, the substrates and wall assemblies, as well as mechanical and technological issues. A multidisciplinary approach is therefore needed, and different skills have to be joined together right from the early design phase in order to optimize and balance all the aspects that are involved. In this framework, a research project has been carried out in Turin (North West Italy), with the aim of developing a novel VGMS, constituted by a modular box covered with vegetation, made up of recycled/natural and highly performing materials from the energy/environmental point of view. After the design phase, the actual performance of the VGMS was assessed, through laboratory and long-term in field monitoring, and at the same time, the technological issues, biometric parameters, and the acoustic, thermal and mechanical aspects were investigated.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK, ZRSKP
The importance of green areas is increasingly considered for the restoration of urban ecosystems. Worldwide studies concerning people needs in urban landscapes have underlined the fundamental role ...carried out by greening in the cities not only for social and aesthetical reasons, but also for environmental aims. Problems like soil erosion, river flooding, lack of biodiversity are frequent in urban contexts and, in the last decade, ecological planning and design methods were often applied. In particular, the application of bioengineering and ecological principles at the urban scale represents an interesting novelty. For this purpose an interdisciplinary approach is needed, agronomists and architects have to work together in order to solve both ecological and technical problems. Therefore, in this study authors looked at the use of bioengineering techniques in some recent interesting examples in urban green areas in Piedmont (north-western Italy). The proper employ of methods (seeding; plantations, rock walls, piling walls), materials (wood, stone, geotextiles, biotextiles) and species (grasses and herbs, shrubs and trees) were analysed. Differences from criteria used in mountain contexts, advantages and limits of these techniques in urban areas and their possible application along banks of rivers, slopes, roads, footpaths or bike paths were pointed out.
T cells in multiple myeloma (MM) patients are highly susceptible to activation with the anti-CD3 monoclonal antibody (mAb) OKT3. When short-term OKT3 stimulation is carried out on bone marrow ...mononuclear cells (BMMC), large numbers of CD3+ CD25+ HLA-DR+ cells are rapidly generated and autologous malignant plasma cells are killed. OKT3 may thus be exploited in autologous bone marrow transplantation (ABMT) to purge residual plasma cells and simultaneously activate T cells to induce graft-versus-leukemia-like (GVL-like) activity upon reinfusion. However, the possible impact of ex-vivo short-term OKT3 stimulation on haematological recovery is unknown. The aim of this work was to investigate the effect of OKT3 stimulation in vitro on autologous haemopoietic progenitor cells (HPC) of MM patients. Colony formation by granulocyte-macrophage progenitor cells (granulocyte-macrophage colony-forming units, CFU-GM) was highly suppressed, although supernatants of OKT3-activated T cells contained up to 2,500 pg/ml of granulocyte-macrophage colony-stimulating factor (GM-CSF). T cell depletion completely prevented this suppression. Neutralizing antibodies against TNF-alpha, TNF-beta and IFN-gamma (which are also produced by OKT3-activated MM T cells) did not prevent it, and Transwell cultures showed that cell-to-cell contact was the main mechanism involved. OKT3-activated T cells also suppressed erythroid burst-forming units (BFU-E) and CFU-GM generation from HPC responsible for long-term maintenance of in vitro myelopoiesis. When tested on normal allogeneic BM, MM supernatants of OKT3-stimulated BMMC partially suppressed the generation of day 7 CFU-GM, but had no effect on day 14 CFU-GM. These data indicate that short-term stimulation of BMMC with OKT3 can be used to generate anti-tumour effector T cells for autologous adoptive immunotherapy. It is not a feasable approach for ex-vivo purging and activation procedures in ABMT because of its potent inhibition of autologous haemopoiesis.
A large expansion of activated T cells (CD3+CD25+) with the potential to act as anti-tumour effector cells is inducible in multiple myeloma (MM) patients by culturing bone marrow mononuclear cells ...(BMMCs) with the anti-CD3 monoclonal antibody (mAb) OKT3. The aim of this study was to provide a greater characterization of CD3-activated T cells. On day 6, most T cells coexpressed the CD11a, CD18, CD54, CD45R0 antigens and consisted of activated (CD25+) CD4+ and CD8+ cells in nearly equal proportions. Kinetics studies showed that CD4+CD25+ cells proliferated more rapidly and peaked earlier than CD8+CD25+ cells. When experiments were performed with purified subpopulations by removing CD4+ cells (resulting in CD8+ BMMCs) or by removing CD8+ cells (resulting in CD4+ BMMCs). T-cell activation and autologous plasma cell decrease were observed in CD4+ BMMCs only. Transwell cultures showed that CD4 help was necessary to make CD8+ BMMCs susceptible to CD3 stimulation. Relevant amounts of IL-2 were found in the supernatants of CD4+ BMMCs cultures, whereas no secretion of IL-4 was detected, indicating a Th1-like profile of CD3-activated CD4+ cells. These data indicate that CD4+ cells proliferate earlier and provide optimal help to induce the subsequent expansion of CD8+ cells after CD3 stimulation of MM BMMCs. Adequate stimulation of CD4+ cells is therefore essential in any strategy aiming to recover T-cell-mediated immunity in MM.
CD3 engagement has been used as a surrogate for antigen-specific stimulation to trigger T cell effector functions. Exogenous IL-2 has been used to prolong and amplify CD3-induced T cell activation. ...Previous studies have shown that CD3 reactivity is increased in cancer patients with preactivated (> 10% HLA-DR+) T cells in the peripheral blood. In this study, we report 9 courses of a single infusion of anti-CD3 mAb (OKT3) followed by continuos infusion of intermediate dose IL-2 in 4 cancer patients 2 multiple myeloma (MM), 1 B-cell lymphoma (NHL), 1 metastatic melanoma (ME) with advanced disease and > 10% HLA-DR+ T cells in the peripheral blood. An increase of lymphocytes, equally distributed between CD4+ and CD8+ subsets, was observed during treatment. Activation was phenotypically documented by the emergence of CD25+ cells in the peripheral blood. Unexpectedly, functional studies including proliferation to mitogens (PHA, OKT3) and cytotoxicity assays (NK and LAK activities) did not parallel phenotypic data and a slight decrease of all functions was observed after OKT3 and IL-2 treatment.
OKT3 and IL-2 infusions were well tolerated and no limiting toxicity was observed. The treatment did not revert tumor progression in the 2 patients with progressive disease (NHL, ME) and had only minimal effects in the 2 MM patients with stable disease. These data indicate that the sequential administration of OKT3 and IL-2 had no anti-tumor activity in this small series of patients with advanced cancer who were selected for treatment because of an increased number of HLA-DR+ T cells in the peripheral blood. A discrepancy was observed between the emergence of CD25+ T cells and the clinical outcome.
Reconstruction of the heel is a complex problem for which many methods have been proposed. The authors describe a method of applying a medial plantar flap with particular reference to special ...clinical situations of a vascular nature. Based on their clinical experience, they recommended the medial plantar flap as the method of choice in the reconstruction of the heel, particularly in view of the trophic, mechanical and sensory features of the transposed skin and the absence of a significant morphological or functional deficit in the donor site. Moreover, the method involves a single surgical stage and a brief period of hospitalisation.
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