Raman imaging is a hyperspectral approach able to provide information on the spatial distribution of a particular biochemical feature without the use of any staining or sample processing. The ...extraction of the relevant information from the large dataset obtained however is a laborious and complex task that still requires the development of robust chemometric approaches. In this paper, we propose a general framework for analyzing data acquired by a commercial Raman spectrometers. This framework is based both on exploiting spectral information and unsupervised clustering, in order to clearly identify the borders and the compositions of different regions of interest. Finally, we describe an efficient GPU-based parallelization, which ensures a fast image classification.
Compressed ".feather" files including the entire dataset of 319 Raman maps of the same number of cells from 19 patients affected by nine distinct leukemia subtypes.Raw data have been pre-processed as ...follows using custom software (LabVIEW, National Instruments Corp., TX): a) cosmic rays removal by singular value decomposition (SVD); b) camera offset subtraction; c) CCD response correction (intensity and etaloning) using a tungsten halogen light with known emission (Avalight-HAL, Avantes BV, NL)); d) wavenumber calibration using the zero-wavenumber laser line, toluene and argon-mercury emission (CAL-2000, Ocean Optics, Germany); e) denoising by SVD.More details in the open access published article and supplementary material (10.1021/acs.analchem.4c00787).
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In this paper, a straightforward method based on elastic light scattering is shown to provide a sensitive and reliable tool for the quantitative determination of receptor-ligand interactions that ...occur at the surface of suitably designed core-shell nanoparticles. The assay makes use of monodisperse nanocolloids that have minimal optical contrast with the aqueous environment. By properly coating the particles with an appropriate receptor and unreactive amphiphiles, we developed a hybrid system that combines the availability of standard ligands with the necessary bioinvisibility towards the accidental adsorption of nonspecific macromolecules. This system has been used for the study of the interactions between small molecules (Vancomycin - Lipid 2), and between proteins (Avidin - biotinnylated IgG.
Cutaneous squamous cell carcinoma (cSCC) is the second most prevalent form of skin cancer, showing a rapid increasing incidence worldwide. Although most cSCC can be cured by surgery, a sizeable ...number of cases are diagnosed at advanced stages, with local invasion and distant metastatic lesions. In the skin, neurotrophins (NTs) and their receptors (CD271 and Trk) form a complex network regulating epidermal homeostasis. Recently, several works suggested a significant implication of NT receptors in cancer. However, CD271 functions in epithelial tumors are controversial and its precise role in cSCC is still to be defined.
Spheroids from cSCC patients with low-risk (In situ or Well-Differentiated cSCC) or high-risk tumors (Moderately/Poorly Differentiated cSCC), were established to explore histological features, proliferation, invasion abilities, and molecular pathways modulated in response to CD271 overexpression or activation in vitro. The effect of CD271 activities on the response to therapeutics was also investigated. The impact on the metastatic process and inflammation was explored in vivo and in vitro, by using zebrafish xenograft and 2D/3D models.
Our data proved that CD271 is upregulated in Well-Differentiated tumors as compared to the more aggressive Moderately/Poorly Differentiated cSCC, both in vivo and in vitro. We demonstrated that CD271 activities reduce proliferation and malignancy marker expression in patient-derived cSCC spheroids at each tumor grade, by increasing neoplastic cell differentiation. CD271 overexpression significantly increases cSCC spheroid mass density, while it reduces their weight and diameter, and promotes a major fold-enrichment in differentiation and keratinization genes. Moreover, both CD271 overexpression and activation decrease cSCC cell invasiveness in vitro. A significant inhibition of the metastatic process by CD271 was observed in a newly established zebrafish cSCC model. We found that the recruitment of leucocytes by CD271-overexpressing cells directly correlates with tumor killing and this finding was further highlighted by monocyte infiltration in a THP-1-SCC13 3D model. Finally, CD271 activity synergizes with Trk receptor inhibition, by reducing spheroid viability, and significantly improves the outcome of photodynamic therapy (PTD) or chemotherapy in spheroids and zebrafish.
Our study provides evidence that CD271 could prevent the switch between low to high-risk cSCC tumors. Because CD271 contributes to maintaining active differentiative paths and favors the response to therapies, it might be a promising target for future pharmaceutical development.
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Cutaneous squamous cell carcinoma (cSCC) represents the second most frequent skin cancer,recently showing a rapid increase in incidence worldwide, with around >1 million cases/year in theUnited ...States and 2500 deaths 1 ....
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