Response surface methodology was used to estimate the optimum extraction parameters, for which the total phenolic content and total flavonoid content of
Avicennia officinalis
L. extract were the ...highest. Based on the 3D surface plots and regression analysis of the independent variables, the optimal conditions for the ultrasound-assisted extraction were as follows: methanol content, 55.27%; liquid-to-solid ratio, 14:1 (mL/g, v/w); temperature, 48.8 °C, and time, 9.66 min. Optimal extraction conditions were applied to validate the quantification. The bioactive compounds from the
Avicennia officinalis
L. extract were identified through ultra-fast liquid chromatography coupled to diode array detector for the first time. These methods were applied to quantify phenolic acids and flavonoids from
A. officinalis
extracts collected at six different locations in Vietnam. The results showed that the concentrations of these compounds varied from 0.319 ± 0.022 to 3.524 ± 0.125 mg/g in the methanol extract. The result showed that cinnamic acid is the major compound in
A. officinalis
collected in Vietnam. In the present study, an optimized ultrasound-assisted extraction was first designed to improve the content of phenolic acids and flavonoid compounds from
A. officinalis
. Additionally, the bioactive compounds from the
A. officinalis
extract were identified through UFLC-DAD for the first time.
A simple and green chemistry approach for the preparation of reduced graphene oxide nanosheets was successfully demonstrated through the reduction of graphene oxide (GO) using caffeine as the ...reductant. Without using toxic and harmful chemicals, this method is environmentally friendly and suitable for the large-scale production of graphene. The samples of GO, before and after reduction with caffeine have been characterized by X-ray diffraction, Raman, Fourier transform infrared, X-ray photoelectron spectroscopy, thermogravimetric analysis and transmission electron microscopy.
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DOBA, EMUNI, FIS, FZAB, GEOZS, GIS, IJS, IMTLJ, IZUM, KILJ, KISLJ, MFDPS, NLZOH, NUK, OILJ, PILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, UILJ, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
Herein, supercritical CO
2
(SC-CO
2
) technique has been utilized to extract triterpenoid from Vietnamese Ganoderma lucidum (G. lucidum). Box-Behnken design was utilized to study the simultaneous ...effects between factors on triterpenoid content including extraction pressure, extraction time, and extraction temperature. Triterpenoid content was analyzed by using reversed-phase high-performance liquid chromatography with a diode array detector, using Pursuit XRs C18 reversed-phase column, the highest triterpenoid content was quantitatively analyzed for 2.735 ± 0.007 mg/g dry weight of material under optimal extraction conditions. Gas chromatography-mass spectrometry analysis revealed the presence of various compounds such as fatty acids and squalene in the ethanol extract of G. lucidum. Also, scanning electron microscopy images were used to evaluate material surfaces before and after extraction, thus proving the proposed mechanism of the extraction process by SC-CO
2
. The presence of triterpenoid and its derivatives were validated by the result of Fourier transform infrared spectra. SFE-CO2 extract has strong antioxidant, antibacterial and anti-cancer properties. As a result, SC-CO
2
may be considered as a viable technique for triterpenoid extraction from G. lucidum.
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BFBNIB, GIS, IJS, KISLJ, NUK, PNG, UL, UM, UPUK
Antimicrobial pectin/chitosan composite films have potential as wound dressing and food packaging. However, almost biofilms were synthesized through chemical routes, causing eco-unfriendliness, and ...negative impacts on the environment and human health. Green synthesis of composite films incorporating with biosynthesized AgNPs is therefore necessary to enhance mechanical and antimicrobial activities. This study focused on the influence of biosynthesized AgNPs on characteristics of passion fruit peel pectin/chitosan (P/CH) biofilms. Characterization, mechanical, antimicrobial activities of P/CH/AgNPs biofilms were evaluated. Incorporating AgNPs into P/CH-based films significantly exerted apparent color and optical properties of biofilms. As AgNPs concentration increased, P/CH/AgNPs biofilms had higher water content (20.1%), better water vapor permeability (1.28 × 10–9 g/mm h Pa), but lower mechanical, water solubility (43.98%), swelling (630.29 ± 11.79%) indexes. P/CH/AgNPs biofilms exhibited outstanding antibacterial properties against S. aureus, B. cereus, P. aeruginosa, K. pneumonia. As expected, in vivo results consolidated the evidence of excellent wound closure (100%) of P/CH/AgNPs biofilms after 15 days. Our findings recommend P/CH/AgNPs antibacterial biofilms have promising biomedical applications.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Point-of-care testing (POCT) using rapid diagnostic tests for infectious disease can potentially guide appropriate use of antimicrobials, reduce antimicrobial resistance, and economise use of ...healthcare resources. POCT implementation in private retail settings such as pharmacies and drug shops could lessen the burden on public healthcare. We performed a narrative review on studies of POCTs in low- and middle-income countries (LMICs), and explored uptake, impact on treatment, and feasibility of implementation.
We searched MEDLINE/PubMed for interventional studies on the implementation of POCT for infectious diseases performed by personnel in private retail settings. Data were extracted and analysed by two independent reviewers.
Of the 848 studies retrieved, 23 were included in the review. Studies were on malaria (19/23), malaria and pneumonia (3/23) or respiratory tract infection (1/23). Nine randomised controlled studies, four controlled, non-randomised studies, five uncontrolled interventions, one interventional pre-post study, one cross-over interventional study and three retrospective analyses of RCTs were included. Study quality was poor. Overall, studies showed that POCT can be implemented successfully, leading to improvements in appropriate treatment as measured by outcomes like adherence to treatment guidelines. Despite some concerns by health workers, customers and shop providers were welcoming of POCT implementation in private retail settings. Main themes that arose from the review included the need for well-structured training with post-training certification covering guidelines for test-negative patients, integrated waste management, community sensitization and demand generation activities, financial remuneration and pricing schemes for providers, and formal linkage to healthcare and support.
Our review found evidence that POCT can be implemented successfully in private retail settings in LMICs, but comprehensive protocols are needed. High-quality randomised studies are needed to understand POCTs for infectious diseases other than malaria.
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DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Necrotizing pneumonia (NP) is characterized by destruction of pulmonary tissue, resulting in multiple thin-walled cavities. There are limited reports on NP and parapneumonic effusion cases in ...children associated with Pseudomonas aeruginosa. Currently, there is no consensus regarding the optimal timing for video-assisted thoracoscopic surgery (VATS) following failure of chest tube placement and antibiotic treatment. A healthy 20-month-old child was hospitalized with symptoms of community-acquired pneumonia, progressing to severe NP and parapneumonic effusion. Despite receiving broad-spectrum antibiotics and chest tube placement on the third day of treatment, the condition continued to deteriorate, prompting VATS intervention on the sixth day. The presence of a “split pleural sign“ and extensive lung necrosis on chest computed tomography contributed to initial treatment failure. Multidrug resistance P. aeruginosa was identified through nasal trachea aspiration specimens on the eighth day of treatment, leading to an adjustment in antibiotic therapy to high-dose meropenem and amikacin. Subsequently, the patient became afebrile, showed clinical improvement, and was discharged after 35 days of treatment. Through this case, we aim to emphasize an unusual pathogenic bacteria in the context of NP and the need for standardized surgical interventions in pediatric patients with NP.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Ischemia are common conditions related to lack of blood supply to tissues. Depending on the ischemic sites, ischemia can cause different diseases, such as hindlimb ischemia, heart infarction and ...stroke. This study aims to evaluate how extracellular vesicles (EVs) derived from ETV2 transfected fibroblasts affect endothelial cell proliferation and neovascularization in a murine model of hindlimb ischemia. Human fibroblasts were isolated and cultured under standard conditions and expanded to the 3th passage before use in experiments. Human fibroblasts were transduced with a viral vector containing the ETV2 gene. Transduced cells were selected by puromycin treatment. These cells were further cultured for collection of EVs, which were isolated from culture supernatant. Following co-culture with endothelial cells, EVs were evaluated for their effect on endothelial cell proliferation and were directly injected into ischemic tissues of a murine model of hindlimb ischemia. The results showed that EVs could induce endothelial cell proliferation in vitro and improved neovascularization in a murine model of hindlimb ischemia. Our results suggest that EVs derived from ETV2-transfected fibroblasts can be promising non-cellular products for the regeneration of blood vessels.
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EMUNI, FIS, FZAB, GEOZS, GIS, IJS, IMTLJ, KILJ, KISLJ, MFDPS, NLZOH, NUK, OBVAL, OILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
Psoas abscess (PA) is an uncommon condition in children where pus accumulates in the psoas muscle. The disease symptoms are subtle and nonspecific, often causing delayed diagnosis.
Our case describes ...an unusual manifestation of PA caused by methicillin-resistant Staphylococcus aureus (MRSA) when an 11-year-old male patient was admitted with suspected bloodstream infection from the gastrointestinal tract and bowel obstruction following closed trauma from a bicycle fall. An emergency laparotomy revealed turbid abdominal fluid with pseudomembranes and no apparent cause of peritonitis was found upon observing the entire abdominal cavity. On the fourth day after admission, blood, sputum, and peritoneal fluid cultures were all positive for MRSA. Despite broad-spectrum antibiotic treatment targeting MRSA, the patient's clinical condition did not improve. PA was confirmed via abdominal computed tomography on the ninth-day post-hospitalization with a persistent MRSA bloodstream infection. Successful treatment of PA was achieved through open surgery. The patient's condition improved and was discharged on the 40th day after admission.
PA can present as bowel obstruction. Therefore, PA should be ruled out in patients with bowel obstruction of unknown etiology, especially if the patient has a history of trauma to the hip region.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
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