Geopolymer foams, as a new type of potential eco-friendly building material, are increasingly being discussed in the literature. This study reports the synthesis and characterization of geopolymer ...foams using hydrogen peroxide (H2O2) solution as pore-forming agent and oil as the stabilizing agent. The geopolymer foams with low bulk densities (0.37<ρb<0.74g/cm3), low thermal conductivities (0.11<λ<0.17W/(m.K)), high porosity (66<p<83 vol%), and acceptable compressive strength (0.3<σ<11.6MPa) were successfully fabricated at appropriate conditions. Factors that influence the insulating, mechanical, porous, and microstructural properties were investigated. It was found that the content of the stabilizing agent and the blowing agent had a significant influence on the porous structure and associated foam performance.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK, ZRSKP
Since the outbreak of the SARS-CoV-2 pandemic, there have been intense structural studies on purified viral components and inactivated viruses. However, structural and ultrastructural evidence on how ...the SARS-CoV-2 infection progresses in the native cellular context is scarce, and there is a lack of comprehensive knowledge on the SARS-CoV-2 replicative cycle. To correlate cytopathic events induced by SARS-CoV-2 with virus replication processes in frozen-hydrated cells, we established a unique multi-modal, multi-scale cryo-correlative platform to image SARS-CoV-2 infection in Vero cells. This platform combines serial cryoFIB/SEM volume imaging and soft X-ray cryo-tomography with cell lamellae-based cryo-electron tomography (cryoET) and subtomogram averaging. Here we report critical SARS-CoV-2 structural events - e.g. viral RNA transport portals, virus assembly intermediates, virus egress pathway, and native virus spike structures, in the context of whole-cell volumes revealing drastic cytppathic changes. This integrated approach allows a holistic view of SARS-CoV-2 infection, from the whole cell to individual molecules.
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•Novel bioactive composite scaffold with capability to release loaded platelet-derived growth factor in sustained manner was prepared.•Prepared composite scaffolds exhibited in vitro ...antibacterial and osteoconductive activities.•In vivo accelerated formation of cranial bone with prepared composite scaffold established their possible application in clinical settings.
The repair of bone defects has long been a challenging and significant health question. Here, collagen hydrogel incorporating platelet-derived growth factor (PDGF)-loaded photopolymerizable ZIF-8-PDA nanoparticles (PDGF@ZIF-8-PDA@COL hydrogel) was prepared and perfused into 3D printed poly (lactide-co-glycolide)-tricalcium phosphate (PLGA-TCP) scaffolds. The resulting PDGF@ZIF-8-PDA@COL/PLGA-TCP composite scaffolds were applied as a bone substitute for cranial bone defect repair. The photopolymerizable ZIF-8-PDA nanoparticles had a mean size of 226.2 ± 5.3 nm with photothermal conversion capacity. PDGF@ZIF-8-PDA@COL/PLGA-TCP composite scaffolds showed a slower release of PDGF compared to PDGF release from collagen hydrogels. The composite scaffolds exhibited excellent antibacterial properties and good in vitro osteoconductive capacity. The osteoconductive activities of PDGF@ZIF-8-PDA@COL/PLGA-TCP composite scaffolds were also investigated in a rat cranial bone defect model in vivo by micro-CT imaging, hematoxylin and eosin staining, Masson’s trichrome staining, and immunohistochemical staining of osteogenesis-related markers. The PDGF@ZIF-8-PDA@COL/PLGA-TCP composite scaffolds accelerated cranial bone formation and gradually degraded over time. All these results provided strong evidence that PDGF@ZIF-8-PDA@COL/PLGA-TCP composite scaffolds might be a promising system for bone defect repair.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Cryo-electron tomography and subtomogram averaging (STA) has developed rapidly in recent years. It provides structures of macromolecular complexes in situ and in cellular context at or below ...subnanometer resolution and has led to unprecedented insights into the inner working of molecular machines in their native environment, as well as their functional relevant conformations and spatial distribution within biological cells or tissues. Given the tremendous potential of cryo-electron tomography STA in in situ structural cell biology, we previously developed emClarity, a graphics processing unit-accelerated image-processing software that offers STA and classification of macromolecular complexes at high resolution. However, the workflow remains challenging, especially for newcomers to the field. In this protocol, we describe a detailed workflow, processing and parameters associated with each step, from initial tomography tilt-series data to the final 3D density map, with several features unique to emClarity. We use four different samples, including human immunodeficiency virus type 1 Gag assemblies, ribosome and apoferritin, to illustrate the procedure and results of STA and classification. Following the processing steps described in this protocol, along with a comprehensive tutorial and guidelines for troubleshooting and parameter optimization, one can obtain density maps up to 2.8 Å resolution from six tilt series by cryo-electron tomography STA.
This paper investigates the event-triggered tracking control of the nonstrict-feedback nonlinear system with time-varying disturbances. While the fuzzy logic systems (FLSs) approximate the unknown ...dynamics, an event-triggered compound learning algorithm is originally developed to accurately estimate the total uncertainties. By referring to an event-triggered adaptive model, the control laws are derived without provoking the problem of “algebraic loop,” seeing Remark
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. The command filters are employed to generate the continuous substitutes for both the virtual control laws and their derivatives, so as to solve the recently proposed problem of “jumps of virtual control laws” arising in the backstepping-based event-triggered control (ETC). The triggering condition is constructed to guarantee the similarity between the adaptive model and the original system. Estimation of optimal fuzzy weights and compound disturbances follows from the event-triggered update laws. While the satisfactory learning performance is achieved, the proposed control scheme can guarantee the semi-globally uniformly ultimate boundedness (SGUUB) of all the tracking errors. Finally, a numerical experiment verifies the effectiveness of the proposed control scheme.
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EMUNI, FIS, FZAB, GEOZS, GIS, IJS, IMTLJ, KILJ, KISLJ, MFDPS, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
How thylakoid membranes are generated to form a metabolically active membrane network and how thylakoid membranes orchestrate the insertion and localization of protein complexes for efficient ...electron flux remain elusive. Here, we develop a method to modulate thylakoid biogenesis in the rod-shaped cyanobacterium Synechococcus elongatus PCC 7942 by modulating light intensity during cell growth, and probe the spatial-temporal stepwise biogenesis process of thylakoid membranes in cells. Our results reveal that the plasma membrane and regularly arranged concentric thylakoid layers have no physical connections. The newly synthesized thylakoid membrane fragments emerge between the plasma membrane and pre-existing thylakoids. Photosystem I monomers appear in the thylakoid membranes earlier than other mature photosystem assemblies, followed by generation of Photosystem I trimers and Photosystem II complexes. Redistribution of photosynthetic complexes during thylakoid biogenesis ensures establishment of the spatial organization of the functional thylakoid network. This study provides insights into the dynamic biogenesis process and maturation of the functional photosynthetic machinery.
The Jueluotage area, which is located in the southern branch of the Eastern Tianshan and northeast of the Tarim Basin, represents a vital locality for investigating intracontinental reactivation ...induced by the tectonic events at the Eurasian plate margin. This study applies zircon and apatite (U–Th)/He and apatite fission-track thermochronology to the Jueluotage area in the Eastern Tianshan. Our data and thermal history modelling show that the Jueluotage area experienced Triassic–Early Jurassic (˜240–180 Ma) cooling, reflecting the closure of the North Tianshan Ocean and subsequent far-field effects of collision/accretion of the Qiangtang Block and Kunlun terrane. Following this period of fast cooling, a differential exhumation process occurred between the various tectonic belts in the Jueluotage area. The Aqishan–Yamansu belt was exposed at the surface during the Triassic–Early Jurassic cooling phase and experienced subsequent burial, which continued until Early Cretaceous time when a pulse of exhumation occurred. However, the major fault zones (Kanggurtag ductile shear zone and Aqikkuduk Fault) and Central Tianshan arc have remained tectonically stable since Early Jurassic time. No Cenozoic rapid cooling was recorded by the low-temperature thermochronology results in this study, indicating that much of the Jueluotage area was exhumed to the upper crust in the late Mesozoic period.
LA-ICP-MS U–Pb ages of detrital zircons from clastics of the undated fore-arc sedimentary unit (FAS) along the Shangdan Suture and the Middle–Upper Devonian Liuling Group in the South Qinling belt ...are used to establish the maximum depositional age and provenance of these tectonic units which were deposited on both sides of the Shangdan suture zone between the North China Block (NCB) and South China Block (SCB). The new data and geological evidence show that the FAS was deposited in a fore-arc basin with an exclusive source of the clastics in the North Qinling Belt (NQB). The depositional age of FAS is limited by the youngest U–Pb 455Ma-ages of detrital zircons from clastics and the intrusive age of 435±7Ma of mafic dykes between the Late Ordovician–Early Silurian, while the NCB was still separated from the SCB by the Shangdan Ocean. However, detrital zircon U–Pb ages from six samples of the Middle Devonian Liuling Group indicate sources in both NQB and SCB suggesting pre-Middle Devonian collision of NCB and SCB. All results indicate deposition of the FAS in a forearc setting upon an active continental margin during Late Ordovician–Early Silurian, while the Middle–Upper Devonian Liuling Group represents a marine foreland basin after closure of the Shangdan Ocean. Together with the unconformity between Middle Devonian and pre-Devonian strata, this reveals still evolving subduction and accretion on the southern side of the NQB during the Ordovician-Early Silurian, and the Early Devonian collision between the NCB and SCB.
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► Detrital zircon ages reveal ca. 455–435 Ma sedimentation in a for-arc basin. ► For-arc basin exclusively received clastics from North Qinling (North China Block). ► Collision between South and North China blocks occurred during Early Devonian. ► Middle Devonian foreland basin received clastics from both South and North China blocks.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK
Oxidative stress and inflammation are implicated in the development of sepsis-related acute lung injury (ALI). MicroRNA-1224-5p (miR-1224-5p) plays critical roles in regulating inflammatory response ...and reactive oxygen species (ROS) production. The present study is aimed at investigating the role and underlying mechanisms of miR-1224-5p in sepsis-related ALI. Mice were intratracheally injected with lipopolysaccharide (LPS, 5 mg/kg) for 12 h to induce sepsis-related ALI. To manipulate miR-1224-5p level, mice were intravenously injected with the agomir, antagomir, or matched controls for 3 consecutive days. Murine peritoneal macrophages were stimulated with LPS (100 ng/mL) for 6 h to further validate the role of miR-1224-5p in vitro. To inhibit adenosine 5′-monophosphate-activated protein kinase alpha (AMPKα) or peroxisome proliferator activated receptor-gamma (PPAR-γ), compound C or GW9662 was used in vivo and in vitro. We found that miR-1224-5p levels in lungs were elevated by LPS injection, and that the miR-1224-5p antagomir significantly alleviated LPS-induced inflammation, oxidative stress, and ALI in mice. Conversely, the miR-1224-5p agomir aggravated inflammatory response, ROS generation, and pulmonary dysfunction in LPS-treated mice. In addition, the miR-1224-5p antagomir reduced, while the miR-1224-5p agomir aggravated LPS-induced inflammation and oxidative stress in murine peritoneal macrophages. Further findings revealed that miR-1224-5p is directly bound to the 3′-untranslated regions of PPAR-γ and subsequently suppressed PPAR-γ/AMPKα axis, thereby aggravating LPS-induced ALI in vivo and in vitro. We demonstrate for the first time that endogenous miR-1224-5p is a critical pathogenic factor for inflammation and oxidative damage during LPS-induced ALI through inactivating PPAR-γ/AMPKα axis. Targeting miR-1224-5p may help to develop novel approaches to treat sepsis-related ALI.
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FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SBCE, SBMB, UL, UM, UPUK
A novel passive asymmetric quasi-zero stiffness vibration isolator (AQZS-VI) comprising two linear springs acting in parallel with one negative stiffness element (NSE) is proposed, of which the NSE ...is mainly constructed by the combination of cantilever plate spring and L-shaped lever (CPS-LSL). The static model of the isolator is deduced considering the geometrical nonlinearity of the NSE and the bending deformation of plate spring. The nonlinear stiffness properties of the CPS-LSL and the AQZS-VI, as well as the nonlinear damping properties of the AQZS-VI, are discussed. The absolute displacement transmissibility of the AQZS-VI under base displacement excitation is obtained using harmonic balance method, and the effects of different excitation amplitudes and damping factors on the vibration isolation performance are analyzed. Better than other quasi-zero stiffness vibration isolators (QZS-VI) whose NSEs do not provide supporting force at zero stiffness point, the NSE of the AQZS-VI provides more supporting force than the parallel connected linear springs, which is very beneficial for improving the bearing capacity of the isolator. Compared with a typical symmetric QZS-VI with same damping property, the AQZS-VI has longer stroke with low stiffness and lower peak value of displacement transmissibility. The prototype experiments indicate that the AQZS-VI outperforms the linear counterpart with much smaller starting frequency of vibration isolation and lower displacement transmissibility. The proposed AQZS-VI has great potential for applying in various engineering practices with superior vibration isolation performance.
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EMUNI, FIS, FZAB, GEOZS, GIS, IJS, IMTLJ, KILJ, KISLJ, MFDPS, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
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