Context. Transit spectroscopy is one of the most commonly used methods to characterize exoplanets’ atmospheres. From the ground, these observations are very challenging due to the terrestrial ...atmosphere and its intrinsic variations, but high-spectral-resolution observations overcome this difficulty by resolving the spectral lines and taking advantage of the different Doppler velocities of the Earth, the host star, and the exoplanet. Aims. We analyze the transmission spectrum around the Na I doublet at 589 nm of the extrasolar planet WASP-69b, a hot Jupiter orbiting a K-type star with a period of 3.868 days, and compare the analysis to that of the well-known hot Jupiter HD 189733b. We also present the analysis of the Rossiter-McLaughlin (RM) effect for WASP-69b. Methods. We observed two transits of WASP-69b with the High Accuracy Radial velocity Planet Searcher (HARPS-North) spectrograph (R = 115 000) at the Telescopio Nazionale Galileo (TNG). We perform a telluric contamination subtraction based on the comparison between the observed spectra and a telluric water model. Then, the common steps of the differential spectroscopy are followed to extract the transmission spectrum. The method is tested with archival transit data of the extensively studied exoplanet HD 189733b, obtained with the HARPS-South spectrograph at ESO 3.6 m telescope, and then applied to WASP-69b data. Results. For HD 189733b, we spectrally resolve the Na I doublet and measure line contrasts of 0.72 ± 0.05% (D2) and 0.51 ± 0.05% (D1), and full width half maximum (FWHM) values of 0.64 ± 0.04 Å (D2) and 0.60 ± 0.06 Å (D1), in agreement with previously published results. For WASP-69b only the contrast of the D2 line can be measured (5.8 ± 0.3%). This corresponds to a detection at the 5σ-level of excess absorption of 0.5 ± 0.1% in a passband of 1.5 Å. A net blueshift of ~ 0.04 Å is measured for HD 189733b and no shift is obtained for WASP-69b. By measuring the RM effect, we get an angular rotation of 0.24-0.01+0.02 rad/day and a sky-projected angle between the stellar rotation axis and the normal of orbit plane (λ) of 0.4-1.9+2.0° for WASP-69b. Similar results to those previously presented in the literature are obtained for the RM analysis of HD 189733b. Conclusions. Even if sodium features are clearly detected in the WASP-69b transmission spectrum, more transits are needed to fully characterize the line profiles and retrieve accurate atmospheric properties.
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Aims. Most hot Jupiters are expected to spiral in toward their host stars because the angular momentum of the orbital motion is transferred to the stellar spin. Their orbits can also precess as a ...result of planet-star interactions. Calculations show that both effects might be detected for the very-hot exoplanet WASP-12 b using the method of precise transit-timing over a time span of about 10 yr. Methods. We acquired new precise light curves for 29 transits of WASP-12 b, spannning four observing seasons from November 2012 to February 2016. New mid-transit times, together with those from the literature, were used to refine the transit ephemeris and analyze the timing residuals. Results. We find that the transit times of WASP-12 b do not follow a linear ephemeris with a 5σ confidence level. They may be approximated with a quadratic ephemeris that gives a change rate in the orbital period of (−2.56 ± 0.40) × 10-2 s yr-1. The tidal quality parameter of the host star was found to be equal to 2.5 × 105, which is similar to theoretical predictions for Sun-like stars. We also considered a model in which the observed timing residuals are interpreted as a result of the apsidal precession. We find, however, that this model is statistically less probable than the orbital decay.
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Six extant species of non-human great apes are currently recognized: Sumatran and Bornean orangutans, eastern and western gorillas, and chimpanzees and bonobos 1. However, large gaps remain in our ...knowledge of fine-scale variation in hominoid morphology, behavior, and genetics, and aspects of great ape taxonomy remain in flux. This is particularly true for orangutans (genus: Pongo), the only Asian great apes and phylogenetically our most distant relatives among extant hominids 1. Designation of Bornean and Sumatran orangutans, P. pygmaeus (Linnaeus 1760) and P. abelii (Lesson 1827), as distinct species occurred in 2001 1, 2. Here, we show that an isolated population from Batang Toru, at the southernmost range limit of extant Sumatran orangutans south of Lake Toba, is distinct from other northern Sumatran and Bornean populations. By comparing cranio-mandibular and dental characters of an orangutan killed in a human-animal conflict to those of 33 adult male orangutans of a similar developmental stage, we found consistent differences between the Batang Toru individual and other extant Ponginae. Our analyses of 37 orangutan genomes provided a second line of evidence. Model-based approaches revealed that the deepest split in the evolutionary history of extant orangutans occurred ∼3.38 mya between the Batang Toru population and those to the north of Lake Toba, whereas both currently recognized species separated much later, about 674 kya. Our combined analyses support a new classification of orangutans into three extant species. The new species, Pongo tapanuliensis, encompasses the Batang Toru population, of which fewer than 800 individuals survive.
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•We describe a new species of great apes, the Tapanuli orangutan Pongo tapanuliensis•Genomic analyses corroborate morphological distinctiveness of P. tapanuliensis•P. tapanuliensis comprises the oldest evolutionary lineage in the genus Pongo•With fewer than 800 individuals, P. tapanuliensis is among the most endangered great apes
Nater et al. describe a new great ape species, the Tapanuli orangutan Pongo tapanuliensis. An isolated population from Batang Toru is highly distinct from the northern Sumatran and Bornean species, based on morphological variation, corroborated by population genomic analyses. Fewer than 800 individuals of P. tapanuliensis survive in the wild.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
The CRISPR/Cas9 system is a powerful tool for studying gene function. Here, we describe a method that allows temporal control of CRISPR/Cas9 activity based on conditional Cas9 destabilization. We ...demonstrate that fusing an FKBP12-derived destabilizing domain to Cas9 (DD-Cas9) enables conditional Cas9 expression and temporal control of gene editing in the presence of an FKBP12 synthetic ligand. This system can be easily adapted to co-express, from the same promoter, DD-Cas9 with any other gene of interest without co-modulation of the latter. In particular, when co-expressed with inducible Cre-ER
, our system enables parallel, independent manipulation of alleles targeted by Cas9 and traditional recombinase with single-cell specificity. We anticipate this platform will be used for the systematic characterization and identification of essential genes, as well as the investigation of the interactions between functional genes.
We report the first detection of sodium absorption in the atmosphere of the hot Jupiter WASP-52b. We observed one transit of WASP-52b with the low-resolution Optical System for Imaging and ...low-Intermediate-Resolution Integrated Spectroscopy (OSIRIS) at the 10.4 m Gran Telescopio Canarias (GTC). The resulting transmission spectrum, covering the wavelength range from 522 nm to 903 nm, is flat and featureless, except for the significant narrow absorption signature at the sodium doublet, which can be explained by an atmosphere in solar composition with clouds at 1 mbar. A cloud-free atmosphere is stringently ruled out. By assessing the absorption depths of sodium in various bin widths, we find that temperature increases towards lower atmospheric pressure levels, with a positive temperature gradient of 0.88 + or - 0.65 Kkm super(-1), possibly indicative of upper atmospheric heating and a temperature inversion.
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An abundance of high-dimensional data has meant that L1 penalized regression, known as the lasso, has become an indispensable tool of the practitioner. A feature of the lasso is a “tuning” parameter ...that controls the amount of shrinkage applied to the coefficients. In practice, a value for the tuning parameter is chosen using the method of cross-validation. It is shown that the model that is selected by the lasso can be extremely sensitive to the fold assignment used for cross-validation. A consequence of this sensitivity is that the results from a lasso analysis can lack interpretability. To overcome this model-selection instability of the lasso, a method called the percentile-lasso is introduced. The model selected by the percentile-lasso corresponds to the model selected by the lasso, when the lasso is fitted using an appropriate percentile of the possible “optimal” tuning parameter values. It is demonstrated that the percentile-lasso can achieve substantial improvements in both model-selection stability and model-selection error compared to the lasso. Importantly, when applied to real data the percentile-lasso, unlike the lasso, produces interpretable results, that is, results that are robust to the assignment of observations to folds for cross-validation. The percentile-lasso is easily applied to extensions of the lasso and in the context of penalized generalized linear models.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK
Implantable neural prosthetics devices offer a promising opportunity for the restoration of lost functions in patients affected by brain or spinal cord injury, by providing the brain with a ...non-muscular channel able to link machines to the nervous system. Nevertheless current neural microelectrodes suffer from high initial impedance and low charge-transfer capacity because of their small-feature geometry (Abidian et al., 2010; Cui and Zhou, 2007). In this work we have developed PEDOT-modified neural probes based on flexible substrate capable to answer to the three critical requirements for neuroprosthetic device: efficiency, lifetime and biocompatibility. We propose a simple procedure for the fabrication of neural electrodes fully made of Parylene-C, followed by an electropolymerization of the active area with the conductive polymer PEDOT that is shown to greatly enhance the electrical performances of the device. In addition, the biocompatibility and the very high SNR exhibited during signal recording make our device suitable for long-term implantation.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK
Glucosamine-6-phosphate synthase (GlcN-6-P synthase) is known as a promising target for antimicrobial agents and antidiabetics. Several compounds of natural or synthetic origin have been identified ...as inhibitors of this enzyme. This set comprises highly selective l-glutamine, amino sugar phosphate or transition state intermediate cis-enolamine analogues. Relatively low antimicrobial activity of these inhibitors, poorly penetrating microbial cell membranes, has been improved using the pro-drug approach. On the other hand, a number of heterocyclic and polycyclic compounds demonstrating antimicrobial activity have been presented as putative inhibitors of the enzyme, based on the results of molecular docking to GlcN-6-P synthase matrix. The most active compounds of this group could be considered promising leads for development of novel antimicrobial drugs or antidiabetics, provided their selective toxicity is confirmed.
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DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, UILJ, UKNU, UL, UM, UPUK
Many metabolic inhibitors, considered potential antimicrobial or anticancer drug candidates, exhibit very limited ability to cross the biological membranes of target cells. The restricted cellular ...penetration of those molecules is often due to their highhydrophilicity. One of the possible solutions to this problem is a conjugation of an inhibitor with a molecular organic nanocarrier. The conjugate thus formed should be able to penetrate the membrane(s) by direct translocation, endocytosis or active transport mechanisms and once internalized, the active component could reach its intracellular target, either after release from the conjugate or in an intact form. Several such nanocarriers have been proposed so far, including macromolecular systems, carbon nanotubes and dendrimers. Herein, we present a comprehensive review of the current status of rational design and synthesis of macromolecular organic nanocarrier-drug conjugates, with special attention focused on the mode of coupling of a nanocarrier moiety with a "cargo" molecule through linking fragments of non-cleavable or cleavable type.
The review presents synthetic aspects of carbon nanotube and dendrimer conjugation with drugs and other bioactive molecules.
Structures of several dozen of known antibacterial, antifungal or antiprotozoal agents are based on the amino acid scaffold. In most of them, the amino acid skeleton is of a crucial importance for ...their antimicrobial activity, since very often they are structural analogs of amino acid intermediates of different microbial biosynthetic pathways. Particularly, some aminophosphonate or aminoboronate analogs of protein amino acids are effective enzyme inhibitors, as structural mimics of tetrahedral transition state intermediates. Synthesis of amino acid antimicrobials is a particular challenge, especially in terms of the need for enantioselective methods, including the asymmetric synthesis. All these issues are addressed in this review, summing up the current state‐of‐the‐art and presenting perspectives fur further progress.
Structures of several dozen known antibacterial, antifungal or antiprotozoal agents are based on an amino acid scaffold. In most of them, the amino acid skeleton is crucial for antimicrobial activity, as very often they are structural analogs of amino acid intermediates of various microbial biosynthetic pathways. Synthesis of amino acid antimicrobials is a particular challenge, especially in terms of the need for enantioselective methods, including asymmetric synthesis.
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FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SAZU, SBCE, SBMB, UL, UM, UPUK
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