We found that ibuprofen (IBU) had a potential for releasing serum albumin-bound glycyrrhetic acid (GA). Based on this observation, IBU was used to pretreat samples for the determination of serum GA ...levels by an inhibition ELISA. This method, termed IBU method was evaluated by the recovery of GA from human serum albumin (HSA) or normal human serum (NHS) that contained the exogeneously added GA (37-1000 ng/ml). The mean recovery of GA from HSA and NHS samples treated with IBU were 104.7 and 105.2%, respectively, whereas those without IBU pretreatment were 2.8 and 10.7%, respectively. Comparison of IBU method and chloroform extraction method revealed that the GA content of serum samples pretreated by each method were almost the same. These results suggest that IBU method is useful as a serum processing procedure for the determination of serum GA levels by an inhibition ELISA.
Griseolutein acts as a bactericidal agent, its toxicity decreasing with increase in cell density. There is no evidence that griseolutein acts at a specific stage of cell cycle. Inhibition of ...incorporation of radioactive thymidine into acid-insoluble fraction of cells is marked and rapid, while inhibition of incorporation of uridine also takes place. Incorporation of 32Pi into the acid-insoluble fraction of cells is inhibited while the incorporation into the nucleotide pool is not. Concentration of any of the four deoxyribonucleoside triphosphates in griseolutein-treated cells are similar to or higher than those in untreated cells. No extensive degradation of cellualr DNA is caused by griseolutein. DNA synthesis in plasmolyzed cells in not inhibited by griseolutein.
An approximation for the average delay in queue of an entering customer is presented for the M/G/K queuing model with finite capacity. The approximation is obtained by means of an approximation ...relating a joint distribution of remaining service time to the equilibrium service distribution.
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Morphological and chemical characterization of lipopolysaccharides (LPS) isolated from NAG (non-agglutinable) type Vibrio cholerae was carried out and glycine was found in a significant amount in ...lipid A fraction of LPS. Some distinct differences from cholera vibrios in the chemical composition were revealed.
Repetitive cortical stimulation induced rhythmical masticatory jaw movement and masseteric nerve activity in cats with the spinal cord sectioned at the C2 level. Jaw depression alone did not induce ...any rhythmical jaw movement, but it turned the irregular jaw movement evoked by subthreshold cortical stimulation into the regular jaw movement as evoked by suprathreshold cortical stimulation. The cortically induced rhythmical masseseteric nerve activity could be induced even in immobilized condition and after cerebellectomy, although the rhythmical nerve activity was depressed in amplitude and the rhythm became slower than before immobilization. Tonic jaw depression after immobilization facilitated the tonic activity in the masseteric nerve and also induced a remarkable increase in the amplitude of the cortically evoked rhythmical nerve activity and a slight acceleration of the rhythm, leading to the same masticatory rhythm as before immobilization. It was concluded (1) that the cortically induced masticatory rhythm in cats is basically generated centrally and (2) that the tonic inputs from the muscle spindle in the jaw-closing muscles participate in the rhythm formation by supplying tonic excitatory inputs mainly to the jaw-closer motoneuron and also to the central rhythm generator.
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