Inactivation of enveloped viruses (VSV SFV and SHV-1) by surfactin lipopeptides was dependent on the hydrophobicity, i.e. the number of carbon atoms of the fatty acid, and on the charge of the ...peptide moiety as well as on the virus species. Surfactins with fatty acid chains of 13 carbon atoms showed very low antiviral activity in comparison to C14 arid C15 isoforms. C15 surfactin monomethyl ester also inactivated SFV which was resistant to the mixture of surfactin isoforms as produced by Bacillus subtilis. In contrast, the dimethyl ester showed no virus-inactivation capacity. Disintegration of viral structures as determined by electron microscopy after inactivation of VSV and SFV was comparable to the liter reduction. The effect of the surfactin isoforms and methyl esters on erythrocyte hemolysis correlated with the virus-inactivation capacity. Surfactins with a fatty acid chain moiety of 15 carbon atoms and one negative charge showed the highest antiviral activity.
The Langmuir–Blodgett (LB) technique was employed to produce thin LB films using an amphiphilic calix-4-resorcinarene onto different substrates such as quartz, gold coated glass and quartz crystals. ...The characteristics of the calix LB films are assessed by UV–visible, quartz crystal microbalance (QCM) and surface plasmon resonance (SPR) measurements. UV–vis and QCM measurements indicated that this material deposited very well onto the solid substrates with a transfer ratio of >0.95. Using SPR data, the thickness and refractive index of this LB film are determined to be 1.14
nm/deposited layer and 1.6 respectively. The sensing application of calixarene LB films towards volatile organic vapors such as chloroform, benzene, toluene and ethanol vapors is studied by the SPR technique. The response of this LB film to saturated chloroform vapor is much larger than for the other vapors. The response is fast and fully recoverable. It can be proposed that this sensing material deposited onto gold coated glass substrates has a good sensitivity and selectivity for chloroform vapor. This material may also find potential applications in the development of room temperature organic vapor sensing devices.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK, ZRSKP
We present the results of a search for untriggered gamma-ray burst (GRB) afterglows with the Robotic Optical Transient Search Experiment-III (ROTSE-III) telescope array. This search covers ...observations from 2003 September to 2005 March. We have an effective coverage of 1.74 deg super(2) yr for rapidly fading transients that remain brighter than 617.5 mag for more than 30 minutes. This search is the first large-area survey to be able to detect typical untriggered GRB afterglows. Our background rate is very low and purely astrophysical. We have found four previously unknown cataclysmic variables (CVs) and one new flare star. We have not detected any candidate afterglow events or other unidentified transients. We can place an upper limit on the rate of fading optical transients with quiescent counterparts dimmer than 620th magnitude at a rate of less than 1.9 deg super(-2) yr super(-1) with 95% confidence. This places limits on the optical characteristics of off-axis (orphan) GRB afterglows. As a by-product of this search, we have an effective 652 deg super(2) yr of coverage for very slowly decaying transients, such as CVs. This implies an overall rate of outbursts from high Galactic latitude CVs of 0.1 deg super(-2) yr super(-1).
We present the unfiltered ROTSE-III light curve of the optical transient associated with GRB 050319 beginning 4 s after the cessation of g-ray activity. We fit a power-law function to the data using ...the revised trigger time given by Chincarini and coworkers, and a smoothly broken power-law to the data using the original trigger disseminated through the GCN notices. Including the RAPTOR data from Wo zniak and coworkers, the best-fit power-law indices are a = -0.854 c 0.014 for the single power-law and a sub(1) = -0.364 super(+) sub(-) super(0) sub(0) super(.) sub(.) super(0) sub(0) super(2) sub(1) super(0) sub(9), a sub(2) = -0.881 super(+) sub(-) super(0) sub(0) super(.) sub(.) super(0) sub(0) super(3) sub(3) super(0) sub(1), with a break at t sub(b) = 418 super(+) sub(-) super(3) sub(3) super(1) sub(0) s for the smoothly broken fit. We discuss the fit results, with emphasis placed on the importance of knowing the true start time of the optical transient for this multipeaked burst. As Swift continues to provide prompt GRB locations, it becomes more important to answer the question, "when does the afterglow begin?" in order to correctly interpret the light curves.
Within this article, we report the characterization and organic vapor sensing properties of Langmuir–Blodgett (LB) thin films of calix8arenes. Surface pressure–area isotherms show that very stable ...monolayers are formed at the air–water interface. The LB film could be deposited onto different substrates which allowed the films to be characterized by UV, quartz crystal microbalance (QCM), surface plasmon resonance (SPR) and atomic force microscopy (AFM). The results indicate that good quality, uniform LB films can be prepared with transfer ratios of over 0.95. QCM results showed that the deposited mass of calix8arene monolayer onto a quartz crystal decreased from 693 to 204
ng as the number of layers is increased. AFM studies showed a smooth, and void free surface morphology with a rms value of 1.202
nm. The sensing abilities of this LB film towards the development of room temperature organic vapor sensing devices are also studied. Responses of the LB films to various vapors are fast, large, and reversible. It was found that the obtained LB film is significantly more sensitive to chloroform than other vapors. It can be concluded that this molecule could have a potential application in the research area of room temperature vapor sensing devices.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK, ZRSKP
We report on a complete set of early optical afterglows of gamma-ray bursts (GRBs) obtained with the Robotic Optical Transient Search Experiment (ROTSE-III) telescope network from 2005 March through ...2007 June. This set is comprised of 12 afterglows with early optical and Swift/X-Ray Telescope observations, with a median ROTSE-III response time of 45 s after the start of {gamma}-ray emission (8 s after the GCN notice time). These afterglows span 4 orders of magnitude in optical luminosity, and the contemporaneous X-ray detections allow multi-wavelength spectral analysis. Excluding X-ray flares, the broadband synchrotron spectra show that the optical and X-ray emission originate in a common region, consistent with predictions of the external forward shock in the fireball model. However, the fireball model is inadequate to predict the temporal decay indices of the early afterglows, even after accounting for possible long-duration continuous energy injection. We find that the optical afterglow is a clean tracer of the forward shock, and we use the peak time of the forward shock to estimate the initial bulk Lorentz factor of the GRB outflow, and find 100 {approx}< {gamma}{sub 0} {approx}< 1000, consistent with expectations.
In this study, we examined hamster polyomavirus (HaPyV) major capsid protein VP1-derived virus-like particles (VLPs) as a carrier for a human tumor-associated cytotoxic T lymphocyte (CTL) epitope. ...The VP1 tolerated the insertion of an HLA-*A2-restricted CTL epitope from human mucin 1 (MUC1) into two sites independently and simultaneously, without interfering with assembly of chimeric VLPs. Chimeric VLPs did not differ in the entry pathway or maturation potential of human dendritic cells (hDCs) compared to unmodified VLPs. Recently we demonstrated that immunization of BALB/c mice with chimeric VLPs harboring two MUC1 insertions resulted in the generation of MUC1-specific monoclonal antibodies. Here we demonstrate that the monoclonal antibodies generated react specifically with human tumor cells. Co-cultivation of chimeric VLP-primed hDCs with autologous peripheral blood leukocytes resulted in the activation of MUC1 epitope-specific CD8(+) T cells. This was evidenced by IFN-gamma secretion of an expanded MUC1-specific CD8(+) T-cell pool. The induction of epitope-specific T cells in a human in vitro model and of murine MUC1-reactive antibodies in vivo indicate the potential of chimeric HaPyV VP1-derived VLPs as a delivery vehicle for immunotherapeutic targets.
We generated highly immunogenic virus-like particles that are based on the capsid protein VP1 of the hamster polyomavirus (HaPV-VP1) and harbor inserted foreign epitopes. The HaPV-VP1 regions ...spanning amino acids 81–88 (position 1), 222/223 (2), 244–246 (3), and 289–294 (4) were predicted to be surface exposed. An epitope of the pre-S1 region of the hepatitis B virus (designated S1; amino acid sequence DPAFR) was introduced into the predicted positions of VP1. All VP1/S1 fusion proteins were expressed in yeast and generated virus-like particles. Immunoassays using the S1-specific monoclonal antibody MA18/7 and immunization of C57Bl6 mice with different VP1/S1 constructs showed a pronounced reactivity and a strong S1-specific antibody response for particles carrying the insert in position 1, 2, 1+2, and 1+3. Our results suggest that HaPV-VP1 represents a highly flexible carrier moiety for the insertion of foreign sequences offering a broad range of potential uses, especially in vaccine development.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Ever since the 1999 Kocaeli earthquake, in which the Kandilli Observatory and Earthquake Research Institute (KOERI) was not able to correctly reflect the magnitude size in its preliminary report ...because of the saturation effect, a rapid and accurate determination of the earthquake becomes a very important issue. Therefore, in the framework of this study, an automatic determination of the moment magnitude was performed by using the displacement spectra of selected earthquakes in the Marmara Region. For this purpose, 39 three-component broadband stations from KOERI seismic network which recorded 174 earthquakes with magnitudes 2.5 ≤
M
≤ 5.0 in between 2006–2009 were used. Due to the importance of quality factor in determination of the moment magnitude with spectral analysis method, the quality factor was calculated for the whole region in the beginning. Source spectrum which was obtained by converting the velocity records to displacement spectra and moment magnitudes of earthquakes were determined by fitting this spectrum to classical Brune model. For this aim, an automatic procedure was utilized which based on minimizing the differences between observed and synthetic source spectra identified by the S waves. Besides moment magnitude and location parameters, some source parameters such as seismic moment, spectral level, corner frequency and stress drop were also calculated. Application of the method proves that determining the seismic moment from the source spectra is applicable not only for earthquakes with small magnitude but also moderate earthquakes as well.
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EMUNI, FIS, FZAB, GEOZS, GIS, IJS, IMTLJ, KILJ, KISLJ, MFDPS, NLZOH, NUK, OBVAL, OILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
For xenotransplantation, the transplantation of animal cells, tissues and organs into human recipients, to date, pigs are favored as potential donors. Beside ethical, immunological, physiological and ...technical problems, the microbiological safety of the xenograft has to be guaranteed. It will be possible to eliminate all of the known porcine microorgansims in the nearby future by vaccinating or specified pathogen-free breeding. Thus, the main risk will come from the porcine endogenous retroviruses (PERVs) which are present in the pig genome as proviruses of different subtypes. PERVs will therefore be transmitted, with the xenograft, to the human recipient. PERVs can infect numerous different types of human primary cells and cell lines in vitro and were shown to adapt to these cells by serial passaging on uninfected cells. Furthermore, PERVs have high homology to other retroviruses, such as feline leukemia virus (FeLV) or murine leukemia virus (MuLV), which are known to induce tumors or immunodeficiencies in the infected host. To evaluate the potential risk of a trans-species transmission of PERV in vivo, naive and immunosuppressed rats, guinea pigs and minks were inoculated with PERV and screened over a period of 3 months for an antibody reaction against PERV proteins or for the integration of proviral DNA into the genomic DNA of the host's cells. Furthermore, we inoculated three different species of non-human primates, rhesus monkey (
Macaca mulatta), pig-tailed monkey (
Macaca nemestrina) and baboon (
Papio hamadryas) with high titers of a human-adapted PERV. To simulate a situation in xenotransplantation, the animals received a daily triple immunosuppression using cyclosporine A, methylprednisolone and RAD, a rapamycin derivative, presently under development by Novartis. None of the small laboratory animals or the non-human primates showed production of antibodies against PERV or evidence of integration of proviral DNA in blood cells or cells of several organs, 3 months after virus inoculation, despite the observation that cells of the animals used in the experiment were infectible in vitro. This apparent difference in the outcome of the in vitro and the in vivo data might be explained by an efficient elimination of the virus by the innate or adaptive immunity of the animals.
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