Enterospora epinepheli is an intranuclear microsporidian parasite causing serious emaciative disease in hatchery‐bred juvenile groupers (Epinephelus spp.). Rapid and sensitive detection is urgently ...needed as its chronic infection tends to cause emaciation as well as white faeces syndrome and results in fry mortality. This study established a TaqMan probe‐based real‐time quantitative PCR assays targeting the small subunit rRNA (SSU) gene of E. epinepheli. The relationship between the standard curve of cycle threshold (Ct) and the logarithmic starting quantity (SQ) was determined as Ct = −3.177 lg (SQ) + 38.397. The correlation coefficient (R2) was 0.999, and the amplification efficiency was 106.4%. The detection limit of the TaqMan probe‐based qPCR assay was 1.0 × 101 copies/μL and that is 100 times sensitive than the traditional PCR method. There is no cross‐reaction with other aquatic microsporidia such as Ecytonucleospora hepatopenaei, Nucleospora hippocampi, Potaspora sp., Ameson portunus. The intra‐assay and inter‐assay showed great repeatability and reproducibility. In addition, the test of clinical samples showed that this assay effectively detected E. epinepheli in the grouper's intestine tissue. The established TaqMan qPCR assays will be a valuable diagnostic tool for the epidemiological investigation as well as prevention and control of E. epinepheli.
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BFBNIB, FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SBCE, SBMB, UL, UM, UPUK
Organic solar cells (OSCs) have made rapid advances in power conversion efficiency during the past decades, which is boosted partly by the various designs of new materials, especially in donor ...materials. However, as one kind of famous fluorescence materials, 4,4′-difluoro-4-bora-3a,4a-diaza-s-indacenes (BODIPY)-based materials are seldom applied in OSCs until its conjugated molecules have been synthesized and designed as donors recently. Here, we designed a series of BODIPY molecules by terminal modulation based on a reported one and probed their potential promising properties for donor materials in OSCs by means of quantum chemical calculations and molecular dynamics simulations. Many important parameters pertaining to short circuit density (J SC) such as capability of light absorption, dipole moment transition between the ground and excited states, hole mobility evaluated on the basis of predicted crystal structures, charge-transfer rate (k inter‑CT), and charge recombination rate (k inter‑CR) computed based on local interface geometries of donor/fullerene selected from molecular dynamics simulation results except for some elemental properties were investigated in detail from a theoretical perspective. The results reveal that the terminal modulation through increasing π-conjugation length is feasible. Notably, it is beneficial to balance the ability of hole transport and charge transfer when considering the π-conjugation length and planarity of donor materials. Finally, we hope that this work can pave the way to the design of potential near-infrared donor materials.
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IJS, KILJ, NUK, PNG, UL, UM
The hot deformation behavior of Al–6.2Zn–0.70Mg–0.30Mn–0.17Zr alloy was investigated by isothermal compression test on a Gleeble–3500 machine in the deformation temperature range between 623 and 773 ...K and the strain rate range between 0.01 and 20 s−1. The results show that the flow stress decreases with decreasing strain rate and increasing deformation temperature. Based on the experimental results, Arrhenius constitutive equations and artificial neural network (ANN) model were established to investigate the flow behavior of the alloy. The calculated results show that the influence of strain on material constants can be represented by a 6th-order polynomial function. The ANN model with 16 neurons in hidden layer possesses perfect performance prediction of the flow stress. The predictabilities of the two established models are different. The errors of results calculated by ANN model were more centralized and the mean absolute error corresponding to Arrhenius constitutive equations and ANN model are 3.49% and 1.03%, respectively. In predicting the flow stress of experimental aluminum alloy, the ANN model has a better predictability and greater efficiency than Arrhenius constitutive equations.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK, ZRSKP
Cyclosporine A (CsA) is an immunosuppressor widely used for the prevention of acute rejection during solid organ transplantation. However, severe nephrotoxicity has substantially limited its ...long‐term usage. Recently, an impaired autophagy pathway was suggested to be involved in the pathogenesis of chronic CsA nephrotoxicity. However, the underlying mechanisms of CsA‐induced autophagy blockade in tubular cells remain unclear. In the present study, we observed that CsA suppressed the activation and expression of transcription factor EB (TFEB) by increasing the activation of mTOR, in turn promoting lysosomal dysfunction and autophagy flux blockade in tubular epithelial cells (TECs) in vivo and in vitro. Restoration of TFEB activation by Torin1‐mediated mTOR inhibition significantly improved lysosomal function and rescued autophagy pathway activity, suppressing TEC injury. In summary, targeting TFEB‐mediated autophagy flux represents a potential therapeutic strategy for CsA‐induced nephrotoxicity.
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FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SBCE, SBMB, UL, UM, UPUK
Background
Female nurses have been considered as a vulnerable population in the context of mental health, due to the nature of their work, which can be stressful and emotionally taxing. Understanding ...the relationship between depressive symptoms and quality of work life (QWL) can contribute to improving mental health and job performance. However, limited studies have focused on the effect of depressive symptoms on QWL in female nurses.
Objectives
The present study aimed to assess the effect of depressive symptoms on female nurses’ QWL using propensity score matching (PSM).
Methods
A cross-sectional, online study using convenience sampling was conducted among 1,401 female nurses in China. PSM was used to minimize the impact of potential confounders between no depressive symptoms and depressive symptoms. Stepwise multiple linear regression analyses were performed on the PSM samples to explore the effects of depressive symptoms on the QWL.
Results
The results revealed there were 33.5% of the female nurses reported depressive symptoms before PSM. And female nurses in this study had a moderate level of QWL before PSM (122.11 ± 18.15), which remained steady after PSM (118.33 ± 18.04). After PSM, the final sample contained 864 female nurses. Stepwise multiple linear regression results indicated that depressive symptoms were the most strongly associated with QWL (
β
= −0.454,
p
< 0.001).
Conclusion
This study highlights the importance of developing mental health plans and psychological interventions for female nurses to maintain mental health and QWL, which is critical to the nursing workforce’s sustainability.
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•Novel ultra-thin (5 nm) MgAl-LDO was fabricated by the cellulose template.•High removal performance was achieved toward toxic Cd2+, Pb2+ and Cu2+.•HMI-substituted LDH and precipitate ...(base or salt) are recognized in product.•Mg-OH moiety of sample is unraveled to play a key role in the removal process.•Cation-exchange mechanism along with deposition is postulated by experiment and DFT.
To remedy the heavy metal ion (HMI) pollution, unravel the relationship of material microstructure and removal performance and clarify relevant mechanism, magnesium-aluminum layered double oxide (MgAl-LDO) has been fabricated via a template method using cellulose. The variously performed characterizations show that ultra-thin flakes of about 5 nm are successfully accessible with the size of 50 ~ 100 nm. They are smaller than the ones prepared without the addition of cellulose. The distinctive structural feature endows the newly-synthesized LDO with large removal capacity for HMIs. Towards Cd2+, Pb2+ and Cu2+, the removal capacity reaches as high as 1422.3, 1336.8 and 1135.4 mg·g−1, respectively. Moreover, the highly achieved durability makes our LDO applicable to treating the polluted water. The mechanism of cation exchange along with deposition has been postulated. The Mg-OH moiety in the sample is found to dominate the HMI removal. The experimental findings are borne out by the first-principle density functional theory calculations on geometries, thermodynamics and electronic structures.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Cellulose@Mg(OH)2 was prepared via a facile method, which has both good shaping ability and high removal capability. The composite can be made into filter to remove heavy metal ion, and the treated ...water meets the potable water criteria.
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•Water treatment agent cellulose@Mg(OH)2 was prepared via a facile method.•Cellulose guides to form ultrathin Mg(OH)2 by non Van der Waals interaction.•Easy shaping property of our material makes HMIs’ separation practical.•High removal capacity and durability are achieved for HMIs’ removal.•Recovered HMIs can be retained on composite fibers and separated from water.
The utilization of renewable biomass cellulose in treating pollution of heavy metal ions (HMIs) is one of overarching and appealing strategies, because it simultaneously satisfies sustainable development and resolves ever-increasing environmental issue. In this regard, the composite cellulose@Mg(OH)2 was prepared via a facile method and explored for its use as water treatment agent. It is demonstrated that the smaller and thinner hexagonal Mg(OH)2 flakes are constructed onto the cellulose substrate by self-assembling; two components are chemically coupled via hydrogen bonds and Mg-Oc (cellulose oxygen) dative bonds; what’s more, the coupling of cellulose with Mg(OH)2 (101) facet is much more preferential than with (001) facet. The resultant composite material shows remarkable HMI removal performance: large capacities of 734.9, 595.8 and 1473.1 mg g−1 for Cd2+, Cu2+ and Pb2+, respectively. Further assisted by good shaping property of the cellulose substrate, the composite is capable of being made into filter, which practically separates HMIs and purifies wastewater with high removal efficiency (99.99%) for Cd2+ even after operating for 110 days and potable water can be obtained. The mechanism is delineated with removal models and characterizations of HMI-recovered products.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Nickle (Ni)-rich layered oxides as promising candidate of high-capacity cathode materials have aroused extensive concern primarily regarding their higher specific capacity. However, the major hurdle ...of Ni-rich cathodes (Ni content ≥0.7) is their poor cycling stability, which often suffers from the release of oxygen, structural degradation and increased impedance during cycling, especially at high voltage or high temperature. Herein, we demonstrated a feasible solid phase method for easy industrialization based on the Al3+/Zr4+ co-doping in LiNi0.72Co0.05Mn0.23O2 (AXZY-NCM) single-crystal cathode. The optimized A0.5Z0.5-NCM single-crystal material exhibited greatly enhanced structural and thermal stability. XRD refinement results indicated that both Al3+ and Zr4+ were mainly located in transition metal (TM) sites, strengthening the layered structure through strong M-O bonds and inhibiting cation mixing. In-situ XRD revealed that the synergistic rivet effect of Al/Zr co-doping alleviates anisotropic lattice stress-strain during charge/discharge process. Accordingly, the A0.5Z0.5-NCM cathode can output a high initial capacity of 177.3 mAh g−1 at 1 C, and captured a more desirable capacity retention of 85.10 % than that of U-NCM (74.33 %) at 1 C over 200 cycles. Even at a high voltage of 4.5 V, its capacity retention can still reach 82.37 %, significantly surpassing the unmodified U-NCM (31.0 %). The above results demonstrated that well-designed dual doping is highly effective for strengthen structure stability and cycling performance of Ni-rich cathodes.
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•Al/Zr doped LiNi0.72Co0.05Mn0.23O2 are designed to reveal the synergistic effect of Al/Zr co-doping on boosting structural stability.•The optimized A0.5Z0.5-NCM achieves the superior thermal stability with their thermal runaway temperature (229.69 oC).•The A0.5Z0.5-NCM shows excellent cycling stability with 85.1% retention after 200 cycles at 2.8∼4.35 V.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Synchronized EIT and ventilator waveform were recorded in three patients with acute exacerbation of chronic obstructive pulmonary disease using PulmoVista500 and V300 (Draeger Medical, Luebeck, ...Germany). By selecting a threshold of 0.25 for ΔRIF, which was defined by searching the optimal value between 0.1 and 0.4 with a step of 0.05 for highest correlation between the annotation and the ΔRIF-based identification, all the 2945 IEE cycles were correctly identified as IEE by EIT. ...EIT is able to characterize the imbalanced ventilation that may be associated with IEE.
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