Cytochrome P450 aromatase (CYP19) plays an important role in steroid homoeostasis by converting androgens to estrogens. To evaluate the effects of benzo(
a)pyrene (BaP), a model carcinogenic PAH and ...AhR ligand, on aromatase mRNA expression and enzyme activity, adult
Fundulus were exposed to water-borne BaP (1 and 10
μg/L) for 15 days, and embryos were exposed to 10
μg/L for 10 days. Effects of BaP were examined by tissue, gender, and season in adults. Constitutively, the sexes did not have significantly different CYP19A2 mRNA levels, however females had higher brain aromatase activity. Female control killifish had more than 700-fold more CYP19A1 mRNA in their gonads compared to males. Within brain tissue of both sexes, there was 100-fold more CYP19A2 mRNA compared to CYP19A1. In ovary, CYP19A1 predominated by approximately 30-fold over the CYP19A2, but in testis there was relatively more CYP19A2. In embryos there was ∼5-fold higher CYP19A2 expression. Due to high inter-individual variability, a significant effect of BaP treatment by gender, season or age was not observed for either aromatase mRNA. However, ovarian aromatase activity was significantly decreased by 10
μg/L BaP, while female brain activity was increased following winter exposure. These findings suggest that the aromatase enzyme is a potential target for disruption of fish developmental and reproductive physiology by BaP.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK
The procedure for learning and creating a new medicine is widely seen as a drawn-out and costly endeavor. Different rational strategies are considered, depending on their requirements, as potential ...ways; nevertheless, techniques to designing drugs based on structure and ligands are well acknowledged as very practical and potent tactics in drug discovery. Computational approaches help decrease the need for Medicinal research with animals, helping to develop fresh, safe therapeutic concepts via rational design and positioning of existing products and supporting pharmaceutical scientists and medicinal chemists during the medication development process. Computer-aided drug discovery (CADD) methods are useful for reducing the time and cost of drug discovery and development and understanding the molecular mechanisms of drug action and toxicity. Molecular docking is a technique that predicts a ligand's binding mode and affinity to a target protein. At the same time, QSAR is a technique that establishes mathematical relationships between the structural features and biological activities of a series of compounds. This study reviews the current state and applications of CADD methods, focusing on molecular docking and quantitative structure–activity relationship (QSAR) techniques. This study reviews the principles, advantages, limitations, and challenges of these methods, as well as some recent advances and examples of their applications in drug discovery for various diseases. The study also discusses the future prospects and directions of CADD methods in the era of big data and artificial intelligence.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Introduction: Children with a Autism spectrum disorders have impairment in the area of communication and social interaction and often display repetitive and non often display. Their parents having ...stress a to manage their children to education and future purpose. This study has assessed the stress level in parents and manage their stress with coping mechanism.
Aims and Objective: 1) To assess the level of stress among parents of children with autism.2) To find association between level of stress and selected demographic variables.
Methods: The data for main study collection was 30 Parents. Description of the tools refers to the explanation of the content of the tool. The researcher lists the number of items and the scoring for each item in the tool. The tool used for the present study is Parenting stress index - Scale. Descriptive and inferential statistics were applied to analyses the data by using SPSS-20 software. We use purposive sampling technique.
Results: The majority of the parents having a moderate stress (83.3%), and severe stress (10%) and mild stress (6.66%) and nonstress level (0.0%). the stress level analysis showed that stress in parents (R=0.321, p=0.00) at significance of 0.05 level present.
Conclusion: The present study concluded that there is a stress level of parents among children having a moderate stress and there is a no any association between the level of stress and selected demographic variable.
Our goal was to study the induction of CYP1B mRNA expression in channel catfish (
Ictalurus punctatus). CYP1B belongs to the cytochrome P450 superfamily of genes, is involved in the oxidation of ...endogenous and exogenous compounds, and could potentially be a useful biomarker in fish for exposure to AhR ligands. The full-length catfish CYP1B cDNA is 2417 nt to the polyA tail and encodes a putative protein of 536 amino acids. It has 67% amino acid similarity to carp and zebrafish CYP1B and 68% similarity to carp CYP1B2. Male channel catfish were collected from three Mississippi Delta sites: Lake Roebuck, Itta Bena; Bee Lake, Thornton; and Sunflower River, Indianola. Total RNA was isolated from wild-caught catfish gill, blood, gonad and liver tissues. Quantitative real-time reverse transcriptase PCR was used to determine relative induction of CYP1B in wild catfish compared to laboratory control and BaP-exposed catfish (20
mg/kg i.p. after 4 days). BaP exposure significantly induced CYP1B message in blood, gonad, and liver of laboratory catfish. In these same tissues of wild catfish from sites with relatively low sediment contaminants, CYP1B message was not statistically increased relative to laboratory control catfish. CYP1B transcript abundance was higher in gills compared to other tissues in both laboratory and wild catfish. When primary cultured gill cells were treated with increasing concentrations of BaP, TCDD, and PCBs 77, 126 and 169, CYP1B mRNA was induced more than 10-fold while PCB153 and 4,4′DDT did not cause significant CYP1B induction. Our results suggest that catfish CYP1B is induced by the classic AhR ligands.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK
Background: Management of patients with acute pulmonary embolism (PE) with imminent hemodynamic collapse and high risk for intracranial bleeding imposes a challenge to clinicians.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
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