With the increasing growth of data dimensionality, feature selection has become a crucial step in a variety of machine learning and data mining applications. In fact, it allows identifying the most ...important attributes of the task at hand, improving the efficiency, interpretability, and final performance of the induced models. In recent literature, several studies have examined the strengths and weaknesses of the available feature selection methods from different points of view. Still, little work has been performed to investigate how sensitive they are to the presence of noisy instances in the input data. This is the specific field in which our work wants to make a contribution. Indeed, since noise is arguably inevitable in several application scenarios, it would be important to understand the extent to which the different selection heuristics can be affected by noise, in particular class noise (which is more harmful in supervised learning tasks). Such an evaluation may be especially important in the context of class-imbalanced problems, where any perturbation in the set of training records can strongly affect the final selection outcome. In this regard, we provide here a two-fold contribution by presenting (i) a general methodology to evaluate feature selection robustness on class noisy data and (ii) an experimental study that involves different selection methods, both univariate and multivariate. The experiments have been conducted on eight high-dimensional datasets chosen to be representative of different real-world domains, with interesting insights into the intrinsic degree of robustness of the considered selection approaches.
Lignocellulosic wastes, such as brewery spent grain (BSG), represent valuable resources from a biorefinery perspective, being sources of both chemical and energy production. Improving degradation ...rates of these recalcitrant feedstocks is a necessity, and bioaugmentation with specialised microbial consortia can be a viable means of achieving this. Six sources of mixed microbial consortia were screened, using biodegradation activity tests, for their capacity to enhance production of both chemicals (volatile fatty acids or ethanol) and energy (biogas) from BSG. The inocula used were anaerobic granular sludge as i) intact granules and ii) crushed granules, iii) bovine rumen fluid, and a number of animal manures including iv) giraffe, v) rhinoceros, and vi) tiger. Amplicon sequencing was used to investigate prokaryotic communities within these inocula on day 0 and day 21. Despite herbivore dung harbouring the largest hydrolytic community, the best solids destruction was achieved using anaerobic granular sludge, either intact (64.8 ± 11.9%) or crushed (63.4 ± 3.7% VS destruction). Granular sludge also resulted in the highest gas production, followed by giraffe manure. Contrastingly, the highest volatile fatty acid accumulation was achieved using rhinoceros and tiger manure, at 3.86 (±0.55) and 3.20 (±0.74) gCOD L−1, respectively. Despite these two manure samples having the highest relative abundances of bacteria associated with cellulose and hemicellulose degradation, they supported the poorest solids destruction, perhaps due to the VFA accumulation therein. Thus, while some inocula aided BSG degradation, further research is required to determine the inhibitors of hydrolysis and therefore BSG degradation.
•Six different inocula evaluated for degradation of lignocellulose materials.•Highest solid degradation achieved using granular sludge.•Highest volatile fatty acid accumulation with rhinoceros and tiger manure.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
The organic acids (OAs) market has been expanding due to their versatility and wide range of uses in several industrial applications, thus prompting a transition from chemical to biological ...approaches to generate such high-value products. Nevertheless, downstream processes to separate OAs from the fermentation broth represent, to date, the main cost in biological OAs manufacturing. With the goal of limiting this issue, the present study investigated OAs separation from a lactic acid (LA)-rich fermentation broth through physical, i.e., using ethyl acetate (EA), and physico-chemical, i.e., using tributyl phosphate (TBP), solvent extraction. In addition, the salting-out effect of different salts on OAs recovery was investigated. Among the investigated conditions, a combination of pure TBP and 40% ammonium sulfate ensured the highest LA extraction efficiency of 65%. Besides, mixing TBP with EA (50:50) enabled to maintain the same LA extraction efficiency while reducing the process costs, limiting the toxicity of the chemicals involved, and obtaining an extracted OAs mix with lower co-metabolites, i.e. volatile fatty acids, compared to using pure TBP. Overall, solvent extraction assisted by salting-out agents were shown to be a promising method to separate OAs from a fermentation broth in liquid form, especially for fermentation processes operated at low pH.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Fermentation processes have been shown to be a good approach to food waste (FW) management. Among the commodities that can be bioproduced by using FW as an organic substrate and exploiting its ...biodegradability, there is lactic acid (LA). LA has gained the interest of research because of its role in the production of polylactic acid plastics. In this study, the influence of the HRT (2-5 days) used during the fermentation of the liquid fraction (∼12-13 g COD/L) of FW on LA yield and concentration was investigated. Moreover, the changes in the chemical composition (in terms of carbohydrates and organic metabolites concentration) of the influent occurring in the feeding tank were monitored and its influence on the downstream fermentation process was examined. High instability characterized the reactor run with the optimal production yield obtained on day 129 at an HRT 2 days with 0.81 g COD/g COD. This study shows the importance of the fluctuating composition of FW, a very heterogeneous and biologically active substrate, for the LA fermentation process. The non-steady state fermentation process was directly impacted by the unstable influent and shows that a good FW storage strategy has to be planned to achieve high and constant LA production.
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BFBNIB, GIS, IJS, KISLJ, NUK, PNG, UL, UM, UPUK
Food waste (FW) generation has become one of the largest environmental concerns for human society. Thanks to its chemical features and its high biodegradability, FW can be used as starting platform ...to produce biocommodities. Lactic acid (LA) is one of those chemicals that is gaining the attention of industry and research for its important role in polylactic acids production. To exploit better the organic content of FW, several FW pretreatments have been proposed in the literature, though none of them were aimed at influencing LA fermentation. Thermal and ultrasonic pretreatment effects on solubilization rates and LA production yields have been investigated in this batch study. The highest solubilization rate was achieved with 30 minutes ultrasonic pretreated FW resulting in a 15% increment in soluble COD (sCOD). The highest LA yield was obtained after 90-minute thermal pretreatment at 80 and 100°C at a yield of 0.49 g LA•g COD−1. This study shows that ultrasonic pretreatment generally performed better than thermal pretreatment when considering the increase in sCOD but caused a reduction in LA concentrations and yields after fermentation with high production of ethanol. The opposite trend was recorded in the thermal pretreated incubations, in which LA was present for 50% of the sCOD with higher LA concentrations of 2.90 g COD•L−1.
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NUK, OILJ, SAZU, UKNU, UL, UM, UPUK
Lactic acid production through fermentation is an established technology, however, improvements are necessary to reduce the process costs and to decrease its market price. Lactic acid is used in many ...industrial sectors and its market has increased in the last decade for its use as the raw material for polylactic acid product. Using food waste as a cheap and renewable substrate, as well as fermentation at uncontrolled pH, helps to make the production cheaper and to simplify the downstream purification process. Lactic acid production at acidic conditions and the role of varying organic loading rate (OLR) and hydraulic retention time (HRT) were tested in two different semicontinuous batch fermentation systems. Reactor performances indicated that lactic acid fermentation was still possible at pH < 3.5 and even up to a pH of 2.95. The highest lactic acid production was recorded at 14-day HRT, 2.14 g VS/L·day OLR, and pH 3.11 with a maximum lactic acid concentration of 8.72 g/L and a relative yield of 0.82 g lactate/g carbohydrates. The fermentation microbial community was dominated by
Lactobacillus
strains, the organism mainly responsible for lactic acid conversion from carbohydrates. This study shows that low pH fermentation is a key parameter to improve lactic acid production from food waste in a semicontinuous system. Acidic pH favored both the selection of
Lactobacillus
strains and inhibited VFA producers from utilizing lactic acid as primary substrate, thus promoting the accumulation of lactic acid. Finally, production yields tend to decrease with high OLR and low HRT, while lactic acid production rates showed the opposite trend.
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EMUNI, FIS, FZAB, GEOZS, GIS, IJS, IMTLJ, KILJ, KISLJ, MFDPS, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
Mass drug administration (MDA) can rapidly reduce the burden of Plasmodium falciparum (Pf). However, concerns remain about its contribution to select for antimalarial drug resistance.
We used Sanger ...sequencing and real-time PCR to determine the proportion of molecular markers associated with antimalarial resistance (k13, pfpm2, pfmdr1 and pfcrt) in Pf isolates collected before (n = 99) and after (n = 112) the implementation of two monthly MDA rounds with dihydroartemisinin-piperaquine (DHAp) for two consecutive years in Magude district of Southern Mozambique.
None of the k13 polymorphisms associated with artemisinin resistance were observed in the Pf isolates analyzed. The proportion of Pf isolates with multiple copies of pfpm2, an amplification associated with piperaquine resistance, was similar in pre- (4.9%) and post-MDA groups (3.4%; p = 1.000). No statistically significant differences were observed between pre- and post-MDA groups in the proportion of Pf isolates neither with mutations in pfcrt and pfmdr1 genes, nor with the carriage of pfmdr1 multiple copies (p>0.05).
This study does not show any evidence of increased frequency of molecular makers of antimalarial resistance after MDA with DHAp in southern Mozambique where markers of antimalarial resistance were absent or low at the beginning of the intervention.
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DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Background An ultrasensitive malaria rapid diagnostic test (RDT) was recently developed for the improved detection of low-density Plasmodium falciparum infections. This study aimed to compare the ...diagnostic performance of the PfHRP2-based Abbott Malaria Ag P. falciparum ultrasensitive RDT (uRDT) to that of the conventional SD-Bioline Malaria Ag P. falciparum RDT (cRDT) when performed under field conditions. Methods Finger-prick blood samples were collected from adults and children in two cross-sectional surveys in May of 2017 in southern Mozambique. Using real-time quantitative PCR (RT-qPCR) as the reference method, the age-specific diagnostic performance indicators of the cRDT and uRDT were compared. The presence of histidine-rich protein 2 (HRP2) and Plasmodium lactate dehydrogenase (pLDH) antigens was evaluated in a subset from dried blood spots by a quantitative antigen assay. pfhrp2 and pfhrp3 gene deletions were assessed in samples positive by RT-qPCR and negative by both RDTs. Results Among the 4,396 participants with complete test results, the sensitivity of uRDTs (68.2; 95% CI 60.8 to 74.9) was marginally better than that of cRDTs (61.5; 95% CI 53.9 to 68.6) (p-value = 0.004), while the specificities were similar (uRDT: 99.0 95% CI 98.6 to 99.2, cRDT: 99.2 95% CI 98.9 to 99.4, p-value = 0.02). While the performance of both RDTs was lowest in greater than or equai to 15-year-olds, driven by the higher prevalence of low parasite density infections in this group, the sensitivity of uRDTs was significantly higher in this age group (54.9, 95% CI 40.3 to 68.9) compared to the sensitivity of cRDTs (39.2, 95% CI 25.8 to 53.9) (p-value = 0.008). Both RDTs detected P. falciparum infections at similar geometric mean parasite densities (112.9 parasites/muL for uRDTs and 145.5 parasites/muL for cRDTs). The presence of HRP2 antigen was similar among false positive (FP) samples of both tests (80.5% among uRDT-FPs and 84.4% among cRDT-FPs). Only one false negative sample was detected with a partial pfhrp2 deletion. Conclusion This study showed that the uRDTs developed by Abbott do not substantially outperform SD-Bioline Pf malaria RDTs in the community and are still not comparable to molecular methods to detect P. falciparum infections in this study setting. Keywords: Malaria, Diagnostics, Rapid diagnostic test (RDT), Ultrasensitive, Low density parasitaemia, HRP2, Plasmodium falciparum, Mozambique
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DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Malaria programmes use Plasmodium falciparum histidine-rich protein-2 (PfHRP2) based rapid diagnostic tests (RDTs) for malaria diagnosis. The deletion of this target antigen could potentially lead to ...misdiagnosis, delayed treatment and continuation of active transmission.
Plasmodium falciparum isolates (n = 1162) collected in Southern Mozambique were assessed by RDTs, microscopy and/or 18SrRNA qPCR. pfhrp2 and pfhrp3 deletions were investigated in isolates from individuals who were negative by RDT but positive by microscopy and/or qPCR (n = 69) using gene-specific PCRs, with kelch13 PCR as the parasite DNA control.
Lack of pfhrp2 PCR amplification was observed in one of the 69 isolates subjected to molecular analysis 1.45% (95% CI 0.3-7.8%).
The low prevalence of pfhrp2 deletions suggests that RDTs will detect the vast majority of the P. falciparum infections. Nevertheless, active surveillance for changing deletion frequencies is required.
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DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
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