Intestinal parasites of dogs and cats may affect their health with a significant zoonotic risk to public health. Therefore, establishing an efficient control program should pass through the ...determination of the diversity, prevalence, and pathogenicity of those parasites. This study aimed to determine the prevalence of intestinal parasites in dogs and cats and proceed to infection comparisons between young and adult animals. The detection of parasites in fecal samples was determined using flotation and immunofluorescent methods across 320 dogs and 64 cats from the Kvarner region in Croatia. The prevalence was calculated for each detected parasite in its host. Differences in prevalence between young animals and adults were analyzed. Parasites were detected in 32 dogs and 34.4% of cats. In total, 12 different genera were detected; Giardia spp. was the most prevalent parasite in both species, infecting 24.7% and 18.8% of investigated dogs and cats, respectively.
spp. and
had a prevalence of (18.4%) and (6.3%), respectively. Prevalences of
,
., and
. were significantly (
< 0.05) higher in puppies compared to adult dogs.
(
) was detected in one puppy. In addition to the first report of
, a relatively high prevalence of intestinal parasites in dogs and cats in the Kvarner region of Croatia was recorded, posing a potential zoonotic risk.
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IZUM, KILJ, NUK, PILJ, PNG, SAZU, UL, UM, UPUK
Posterolateral lumbar fusion (PLF) is a commonly performed surgical procedure for the treatment of pathological conditions of the lumbosacral spine. In the present study, we evaluated an autologous ...bone graft substitute (ABGS) containing rhBMP6 in autologous blood coagulum (ABC) and synthetic ceramics used as compression resistant matrix (CRM) in the rabbit PLF model. In the pilot PLF rabbit experiment, we tested four different CRMs (BCP 500–1700 μm, BCP 1700–2500 μm and two different TCP in the form of slabs) which were selected based on achieving uniform ABC distribution. Next, ABGS implants composed of 2.5 mL ABC with 0.5 g ceramic particles (TCP or BCP (TCP/HA 80/20) of particle size 500–1700 μm) and 125 μg rhBMP6 (added to blood or lyophilized on ceramics) were placed bilaterally between transverse processes of the lumbar vertebrae (L5-L6) following exposition and decortication in 12 New Zealand White Rabbits observed for 7 weeks following surgery. Spinal fusion outcome was analysed by μCT, palpatory segmental mobility testing and selected specimens were either tested biomechanically (three-point bending test) and/or processed histologically. The total fusion success rate was 90.9% by both μCT analyses and by palpatory segmental mobility testing. The volume of newly formed bone between experimental groups with TCP or BCP ceramics and the different method of rhBMP6 application was comparable. The newly formed bone and ceramic particles integrated with the transverse processes on histological sections resulting in superior biomechanical properties. The results were retrospectively found superior to allograft devitalized mineralized bone as a CRM as reported previously in rabbit PLF. Overall, this novel ABGS containing rhBMP6, ABC and the specific 500–1700 μm synthetic ceramic particles supported new bone formation for the first time and successfully promoted posterolateral lumbar fusion in rabbits.
•A new ABGS containing rhBMP6 in autologous blood coagulum with synthetic ceramics (500-1700 μm) was tested in PLF rabbit model.•The fusion success rate was 90.9% by microCT analysis, palpatory segmental mobility and biomechanical testing.•This novel ABGS for the first time supports new bone formation and promotes PLF in rabbits.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Extracellular matrix (ECM) has been intensively used in cardio surgery. The main goal of this research was to determine if Achilles tendon healing could be promoted by applying extracellular matrix ...scaffold (CorMatrix®, USA). Sixteen (n = 16) New Zealand white mature rabbits (Oryctolagus cuniculus) were randomly allocated into two groups. Following complete surgical transection, rabbits in group A (ECM applied) (n = 8) had their Achilles tendons reconstructed using both, nylon suture and extracellular matrix scaffold, whereas in group B (without ECM) the tendons were reconstructed using nylon suture only. After four weeks, the rabbits were euthanized and tendon samples harvested and stained with hematoxylin eosin, Mallory, and Gomory and subsequently histologically analyzed according to modified Bonnar scale. Group B had significantly stronger inflammatory response, including abundant cell infiltration and neovascularization. In group A collagen fibers were predominantly found, whereas in group B reticular fibers were more abundant. Extracellular matrix scaffold has been found to have the real potential for promoting tendon healing through accelerating collagen formation, which is crucial for restoring biomechanical properties of a tendon, decreasing peritendineous adhesion formation, and reducing inflammatory edema and subsequently pain.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
The incidence of perinatal mortality of Arabian horses in Croatia is in accordance with the high frequency of lethal and sublethal mutations in Arabian horses in other countries. The objective of ...this study was to investigate the frequency of recessive genes causing lavender foal syndrome (LFS) in three Croatian breeds of Arabian horse and to compare these findings with the literature reports. Pedigree and molecular analyses indicated the existence of three distinct breeds of Arabian horses (Traditional Arabian, Shagya Arab, and Pure Arabian horse) in Croatia. Molecular analyses were performed on 100 blood samples (n = 100) of Arabian horses by analyzing isolated and quality-tested genomic DNA. Polymerase chain reaction-restriction fragment length polymorphism analysis of the MYO5A gene was performed for LFS determination. The proportion of heterozygous carriers and the frequency of the LFS allele were lower in Arabian horse breeds in Croatia than in other reports. The LFS allele was found exclusively in the Pure Arabian breed, supporting the findings of other studies on Egyptian Arabian horses. The results are consistent with the interbreeding of Pure Arabian horses in Croatia with Egyptian Arabian horses. The Traditional Arabian and Shagya Arab breeds showed a lower frequency of the LFS allele. Based on these results, screening of the entire population of Arabian horses in Croatia for mutations responsible for multiple inherited genetic disorders should be included in breeding programs.
•Lavender foal syndrome in the Arabian horse breed population in Croatia.•The allele for lavender foal syndrome was found in 9.62% of tested Arabian horses.•Genetic screening of Arabian horses in Croatia to determine mutations is required.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK, ZRSKP
The dairy sector is essential for the world's food systems, playing a vital role in agricultural production. Yet, mastitis, an inflammation of the mammary gland, affects a considerable portion of the ...dairy cow population annually, leading to reduced productivity and sustainability. Insight into therapeutic protocols is essential for a better understanding of the situation on farms with the aim of developing new or harmonizing existing protocols in the mastitis treatment. This study conducted on dairy farm in Serbia aimed to explore the most commonly used antibiotics during bovine mastitis treatment and their association with daily milk yield. Data from 100 lactating cows, including therapeutic protocols, lactation numbers, mastitis episodes, milk yield, and antibiotic usage, were obtained from a computerized database and statistically analysed. The results demonstrated a high prevalence of narrow‐spectrum antibiotics usage, with the majority of cows treated with combination therapy. The route of antibiotic application did not significantly impact daily milk yield, while treatment duration had no discernible effect. Nonetheless, specific antibiotics were associated with milk yield variations, suggesting the need for careful antibiotic selection and management in mastitis therapy. These findings emphasize the importance of prudent antibiotic use to safeguard animal health and long‐term milk production sustainability.
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BFBNIB, FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SAZU, SBCE, SBMB, UL, UM, UPUK
MUSULIN, A., M. SKULAC, M. VUCKOVIC, A. MARTINOVIC, P. KOSTESIC, N. BRKLJACA BOTTEGARO, M. PECIN, D. VNUK: Effects of diazepam/ketamine and xylazine/ketamine sedation in geese. Vet. arhiv 88, ...835-846, 2018.The objective of this paper was to compare the effects of diazepam/ketamine and xylazine/ketamine combinations administered intramuscularly to geese, by measurement of the duration of individual anaesthesia periods, and, by exploring their effect on the respiratory and cardiovascular systems, to ascertain which combination is safer or better to use in geese. Eight geese received the combination of xylazine and ketamine, eight received the combination of diazepam and ketamine, and six received a 0.9% NaCl solution. Body temperature, breathing and pulse rate were monitored, while biochemical testing and determination of the acid-basic state of venous blood was performed before the administration of the agent, 5 minutes after administration, and after geese began to raise their heads. The geese from the control group were subjected to the additional measurements 35 minutes after saline administration. The period from anaesthetic administration to setting the head on the ground was significantly shorter in geese receiving diazepam/ketamine than in the geese receiving xylazine/ketamine. The time period from setting the head on the ground to the time of the first reaction to pressure on the distal phalanx, and the time from administration to the time of standing up was significantly longer in geese receiving diazepam/ketamine than in geese receiving xylazine/ketamine. The venous CO.sub.2 partial pressure was significantly higher during anaesthesia in the xylazine/ketamine group. According to the results of this study, it may be concluded that the anaesthesia of geese with the diazepam/ketamine combination showed more favourable effects than the anaesthesia with the xylazine/ketamine combination.Key words: anaesthesia; geese; cardiorespiratory effects
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