The zymogram technique was applied to a beta-lactamase neutralization assay with anti-TEM-1 and anti-TEM-2 sera. Both were shown to contain neutralizing antibodies directed towards various ...beta-lactamases of Gram-negative bacteria. The quantitative neutralization allowed classification into five groups of the 28 beta-lactamases used as standards and 61 from clinical isolates. In the first were enzymes such as TEM-1 and TEM-2 including TLE-1, SHV-1, SHV-2, penicillinases of Klebsiella pneumoniae and CTX-1. Partial neutralization distinguished two groups containing the CARB group of enzymes, which are different from PSE-2 and PSE-3, and the MAL penicillinases of Levinea malonatica, which are different from L. amalonatica enzymes. Broad spectrum beta-lactamases of K. oxytoca constituted a unique group of partially neutralized enzymes. Among the beta-lactamases not neutralized by either serum were the plasmid-mediated OXA-enzymes, various species-specific beta-lactamases and cephalosporinases. The antigenic similarities of the enzymes appeared to correlate with the extent of similarities of their catalytic properties, namely those of penicillinases. Such comparisons between the beta-lactamase groups provide an indirect approach to the physiological and structural analysis of established and recently evolved beta-lactamases.
L’activité spatiale en Guyane représente un enjeu économique et stratégique important. Le risque industriel est prégnant
et afin d’y répondre une unité élémentaire spécialisée des pompiers de Paris ...est détachée au sein du Centre spatial
guyanais. Un médecin des armées y est affecté depuis presque vingt ans. Au-delà du soutien médical des sapeurspompiers
et de leurs familles, il assure une astreinte permanente pour faire face aux situations d’urgence au sein du
centre spatial, il participe à l’élaboration des plans de secours et exerce dans les hôpitaux de proximité. Cette mission
intéresse donc les praticiens formés à la médecine d’urgence, à la médecine tropicale et à la gestion de crise. Son
originalité la positionne au centre des compétences du médecin des armées.
Mots-clés : Brigade de sapeurs-pompiers de Paris. Centre spatial Guyanais
The whole blood and plasma viscosity, hematocrit and plasma fibrinogen were studied in 26 diabetic patients (11 with and 15 without retinopathy) and in 25 non-diabetic control subjects. Blood ...viscosity, plasma viscosity and plasma fibrinogen were significantly higher in diabetics than in controls, but no significant differences were found when diabetics with retinopathy were compared to diabetics without retinopathy. The red cell deformability was significantly reduced in diabetics if compared with that of controls; the statistical significance of this reduction was confirmed only in the group of diabetics with retinopathy, but failed when the group of diabetics without retinopathy was considered.
