Alpinia oxyphylla is a homology of medicine and food. This study aims to investigate the dominant chemical composition and explore the antioxidant properties of the ethanol extract of the leaves and ...stems of A. oxyphylla (AOE) on juvenile shrimp, Litopenaeus vannamei. An in vitro test showed that AOE and its dominant chemical composition procyanidin B-2 (1) and epicatechin (2) presented DPPH and ABTS radical scavenging activities. A shrimp feeding supplement experiment revealed that shrimp growth parameters and muscle composition were improved significantly when fed with a 200 mg/Kg AOE additive. Meanwhile, the activities of antioxidant enzymes (CAT, GSH-Px, SOD, and T-AOC) in serum and the liver and the expression of related genes (LvMn-SOD, LvCAT, LvproPo, and LvGSH-Px) were enhanced with various degrees in different AOE additive groups while the content of MDA was significantly decreased. Moreover, the antioxidative effect of AOE additive groups on shrimp was also observed in an acute ammonia nitrogen stress test.
N-substituted bis(diphenylphosphanyl)amine RN(PPh2)2 (PNP; R = CH2CH2CH2Si(OEt)3) and its mononuclear nickel(II) ethanedithiolate complexe RN(PPh2)2Ni(SCH2CH2S) (PNS; R = CH2CH2CH2Si(OEt)3) were ...prepared and used as multi-element flame retardants for epoxy resin (EP). Results of thermogravimetric analysis (TGA) revealed that the incorporation of PNP or PNS restrained the decomposition of EP and also improved the stability of char residues. Moreover, results of limited oxygen index (LOI), vertical burning test (UL-94), microscale combustion calorimeter (MCC), and cone calorimeter tests proved that both PNP and PNS endowed EP with good flame retardancy. Different chemical structures and action mechanisms of PNP and PNS resulted in different degrees of flame retardancy for EP. Although EP/PNP samples caused a little higher LOI values than those of EP/PNS ones, the incorporation of 7 wt% PNS endowed EP with a lower peak of heat release rate (PHRR) and total smoke production (TSP), thereby indicating better flame-retardant and smoke suppression effects. Furthermore, thermogravimetry-Fourier transform infrared spectroscopy (TG-FTIR) and pyrolysis gas chromatography/mass spectrometry (Py-GC/MS) were employed to analyze the pyrolysis behavior of EPs and flame retardants. The morphologies of char layers for EPs were examined by scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDX). In comparison to PNP, PNS promoted the formation of a stronger protective char barrier comprising by P/Si/S/Ni elements and also restrained the effects of flammable gases on EP.
•N-substituted bis(diphenylphosanyl)amine (PNP) and its mononuclear nickel(Ⅱ) ethaneditiolate complexe (PNS) are prepared.•PNS show better flame-retardant effect than PNP due to the additional flame-retardant elements S and Ni.•PNS reveals excellent flame retardant and smoke suppression effects for epoxy resin.•Part of P, S and Si and all of Ni from PNS promote the formation of a protective char barrier for EP.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
The extractant tri-n-butyl phosphate (TBP) was used for the separation of Pu(IV) and U(VI) in the PUREX process, which brings phosphorus pollution problems. The ligands in accordance with the “CHON” ...principle will be helpful to reduce environmental pollution and pressure of subsequent waste treatment. In this work, three environmentally friendly BisDGA ligands in accordance with the “CHON” principle were used for the selective extraction Pu(IV) form U(VI) in 1-octanol system. These ligands showed highly extraction ability for Pu(IV), but hardly extracted U(VI) even under high acidity, which proves that the applicability of these extraction systems for selective separation of Pu(IV) from U(VI). The coordination chemistry of Pu(IV) by slope analyses confirmed that the ligands generated both M:L = 1:1 complexes with Pu(IV). The properties and thermodynamics of the PuL(NO
3
)
4
complexes formed with Pu(IV) and BisDGA ligands were also investigated with the density functional theory (DFT) calculations, and the results verified the relevant experimental conclusion that the affinity of TH
i
-PE-BisDGA for Pu(IV) is better than the other two ligands.
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EMUNI, FIS, FZAB, GEOZS, GIS, IJS, IMTLJ, KILJ, KISLJ, MFDPS, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
CD19-targeted chimeric antigen receptor T-cell (CAR-T) therapy is effective in refractory/relapsed (R/R) B-cell acute lymphoblastic leukemia (B-ALL). This review focuses on achievements, current ...obstacles, and future directions in CAR-T research. A high complete remission rate of 68% to 93% could be achieved after anti-CD19 CAR-T treatment for B-ALL. Cytokine release syndrome and CAR-T-related neurotoxicity could be managed. In view of difficulties collecting autologous lymphocytes, universal CAR-T is a direction to explore. Regarding the high relapse rate after anti-CD19 CAR-T therapy, the main solutions have been developing new targets including CD22 CAR-T, or CD19/CD22 dual CAR-T. Additionally, some studies showed that bridging into transplant post-CAR-T could improve leukemia-free survival. Some patients who did not respond to CAR-T therapy were found to have an abnormal conformation of the CD19 exon or trogocytosis. Anti-CD19 CAR-T therapy for R/R B-ALL is effective. From individual to universal CAR-T, from one target to multi-targets, CAR-T-cell has a chance to be off the shelf in the future.
Limestone is a relatively abundant and low-cost material used for producing calcium oxide as a CO2 adsorbent. However, the CO2 capture capacity of limestone decreases rapidly after multiple ...carbonation/calcination cycles. To improve the CO2 capture performance, we developed a process using limestone to transform the material into a rod Ca-based metal–organic framework (Ca-MOF) via a hydrothermal process with the assistance of acetic acid and terephthalic acid (H2BDC). The structural formation of rod Ca-MOF may result from the (200) face-oriented attachment growth of Ca-MOF sheets. Upon heat treatment, a highly stable porous rod network with a calcined Ca-MOF-O structure was generated with a pore distribution of 50–100 nm, which allowed the rapid diffusion of CO2 into the interior of the sorbent and enhanced the CO2 capture capacity with high multiple carbonation–calcination cycle stability compared to limestone alone at the intermediate temperature of 450 °C. The CO2 capture capacity of the calcined porous Ca-MOF-O network reached 52 wt% with a CO2 capture stability of 80% after 10 cycles. The above results demonstrated that rod Ca-MOF can be synthesized from a limestone precursor to form a porous network structure as a CO2 capture sorbent to improve CO2 capture performance at an intermediate temperature, thus suggesting its potential in environmental applications.
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IZUM, KILJ, NUK, PILJ, PNG, SAZU, UL, UM, UPUK
Monitoring spatial and temporal chemical status of water bodies is crucial to assist environmental policy, identify the chemical fingerprints, and further reduce the source orientated pollutants. ...Elbe River is one of the major rivers affected by anthropogenic activities in vicinity countries. This study assessed the spatiotemporal changes in response to source shift of Cd, Cu, Ni, Pb, and Zn in the suspended particulate matter (SPM) at upstream, midstream, and downstream of the Elbe River reach in Saxony state, Germany. The average contents of trace metals in SPM was found in the order of Zn (676 mg/kg) » Pb (79 mg/kg) > Cu (74 mg/kg) > Ni (48 mg/kg) » Cd (3.2 mg/kg). According to the Mann-Kendall trend test, Cd, Cu, Pb, and Zn showed significant declines over 1998–2016. The results of source apportionment indicate industrial, urban, natural, and historical mining sources influencing the metal contents in the Elbe River of Saxony. The contributions of industrial and urban pollution decreased by 58.2% from 1998 to 2007 to 2008–2016. The contribution of the natural source was steady over the last two decades.
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•Long-term in spatiotemporal changes of metals in the Elbe River had been evaluated.•Most metals showed significant declines over 1998–2016.•Primary source shift of metals was identified by PMF receptor model.•Industrial and urban pollution showed decreasing contribution.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Imidacloprid enters the water environment through rainfall and causes harm to aquatic crustaceans. However, the potential chronic toxicity mechanism of imidacloprid in crayfish has not been ...comprehensively studied. In this study, red claw crayfish (Cherax quadricarinatus) were exposed to 11.76, 35.27, or 88.17 μg/L imidacloprid for 30 days, and changes in the physiology and biochemistry, gut microbiota, and transcriptome of C. quadricarinatus and the interaction between imidacloprid, gut microbiota, and genes were studied. Imidacloprid induced oxidative stress and decreased growth performance in crayfish. Imidacloprid exposure caused hepatopancreas damage and decreased serum immune enzyme activity. Hepatopancreatic and plasma acetylcholine decreased significantly in the 88.17 μg/L group. Imidacloprid reduced the diversity of the intestinal flora, increased the abundance of harmful flora, and disrupted the microbiota function. Transcriptomic analysis showed that the number of up-and-down-regulated differentially expressed genes (DEGs) increased significantly with increasing concentrations of imidacloprid. DEG enrichment analyses indicated that imidacloprid inhibits neurotransmitter transduction and immune responses and disrupts energy metabolic processes. Crayfish could alleviate imidacloprid stress by regulating antioxidant and detoxification-related genes. A high correlation was revealed between GST, HSPA1s, and HSP90 and the composition of gut microorganisms in crayfish under imidacloprid stress. This study highlights the negative effects and provides detailed sequencing data from transcriptome and gut microbiota to enhance our understanding of the molecular toxicity of imidacloprid in crustaceans.
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•Imidacloprid strongly inhibited the growth performance of Cherax quadricarinatus.•Imidacloprid suppressed immunity and caused hepatopancreatic damage in C. quadricarinatus.•Imidacloprid reduces the diversity and destroys the balance in the intestinal flora of crayfish.•Imidacloprid may alter gene expression by affecting intestinal flora.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Abstract
Asymmetric cell division (ACD) is a mechanism used by stem cells to maintain the number of progeny. However, the epigenetic mechanisms regulating ACD remain elusive. Here we show that BRD4, ...a BET domain protein that binds to acetylated histone, is segregated in daughter cells together with H3K56Ac and regulates ACD. ITGB1 is regulated by BRD4 to regulate ACD. A long noncoding RNA (lncRNA), LIBR (LncRNA Inhibiting BRD4), decreases the percentage of stem cells going through ACD through interacting with the BRD4 mRNAs. LIBR inhibits the translation of BRD4 through recruiting a translation repressor, RCK, and inhibiting the binding of BRD4 mRNAs to polysomes. These results identify the epigenetic regulatory modules (BRD4, lncRNA LIBR) that regulate ACD. The regulation of ACD by BRD4 suggests the therapeutic limitation of using BRD4 inhibitors to treat cancer due to the ability of these inhibitors to promote symmetric cell division that may lead to tumor progression and treatment resistance.
Graphical Abstract
Graphical Abstract
Antimicrobial peptides (AMPs), which are widely present in animals and plants, have a broad distribution, strong broad-spectrum antibacterial activity, low likelihood of developing drug resistance, ...high thermal stability and antiviral properties. The present study investigated the effects of adding AMPs from Hermetia illucens larvae on the growth performance, muscle composition, antioxidant capacity, immune response, gene expression, antibacterial ability and intestinal microbiota of Cherax quadricarinatus (red claw crayfish). Five experimental diets were prepared by adding 50 (M1), 100 (M2), 150 (M3) and 200 (M4) mg/kg of crude AMP extract from H. illucens larvae to the basal diet feed, which was also used as the control (M0). After an eight-week feeding experiment, it was discovered that the addition of 100–150 mg/kg of H. illucens larvae AMPs to the feed significantly improved the weight gain rate and specific growth rate of C. quadricarinatus. Furthermore, the addition of H. illucens larvae AMPs to the feed had no significant effect on the moisture content, crude protein, crude fat and ash content of the C. quadricarinatus muscle. The addition of 100–150 mg/kg of H. illucens larvae AMPs in the feed also increased the antioxidant capacity, nonspecific immune enzyme activity and related gene expression levels in C. quadricarinatus, thereby enhancing their antioxidant capacity and immune function. The H. illucens larvae AMPs improved the structure and composition of the intestinal microbiota of C. quadricarinatus, increasing the microbial community diversity of the crayfish gut. Finally, the addition of 100–150 mg/kg of H. illucens larvae AMPs in the feed enhanced the resistance of C. quadricarinatus against Aeromonas hydrophila, improving the survival rate of the crayfish. Based on the aforementioned findings, it is recommended that H. illucens larvae AMPs be incorporated into the C. quadricarinatus feed at a concentration of 100–150 mg/kg.
•Dietary AMPs improved the growth performance and non-specific immunity of crayfish.•Dietary AMPs effectively reduced the abundance of Proteobacteria in crayfish intestine.•Dietary AMPs enhanced the crayfish's resistance to A. hydrophila infection.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
This study was performed to investigate the effects of dietary trehalose on growth, muscle composition, non-specific immune responses, gene expression and desiccation resistance of juvenile red claw ...crayfish (Cherax quadricarinatus). A total of 540 (body weight of 0.41 ± 0.05) crayfish were randomly divided into six groups for a feeding experiment. Six diets with trehalose levels at 0 (Diet 1), 1 (Diet 2), 2 (Diet 3), 5 (Diet 4), 10 (Diet 5) and 15 (Diet 6) g kg−1 were prepared to feed juvenile red claw crayfish for 8 weeks. The results showed that the weight gain rate (WGR) and specific growth rate (SGR) of crayfish in Diet 4, Diet 5 and Diet 6 groups were significantly improved compared with the control group (Diet 1). Muscle crude protein contents of crayfish fed Diet 4, Diet 5 and Diet 6 were significantly higher than those of the control group. The activities of superoxide dismutase (SOD) and alkaline phosphatase (AKP) in hepatopancreas and hemolymph of crayfish for Diet 4, Diet 5, and Diet 6 groups were significantly increased while malondialdehyde (MDA) content was significantly reduced when compared with the control. The total antioxidant capacity (T-AOC), catalase (CAT) and glutathione peroxidase (GPx) activities in the hepatopancreas and hemolymph of crayfish fed Diet 5 and Diet 6 were significantly higher than those in the control group. However, acid phosphatase (ACP) activity was not significantly different among all experimental groups. The hepatopancreas and intestine trehalose contents of crayfish showed an upward trend with the increase of dietary trehalose levels. Compared with the control group, supplementation of 5–15 g kg−1 trehalose in the feed up-regulated the expression levels of GPx, C-type lysozyme (C-LZM), antilipolysacchride factor (ALF), facilitated trehalose transporter homolog isoform X2 (Tret1-2) and facilitated trehalose transporter isoform X4 (Tret1-4) mRNA. In addition, supplementation of 5–15 g kg−1 trehalose in the feed could improve the survival rate of red claw crayfish under desiccation stress. These results suggested that supplementation of 5–15 g kg−1 trehalose in feed could significantly improve the growth performance, muscle protein, non-specific immunity and desiccation resistance of juvenile red claw crayfish.
•Dietary trehalose improved growth performance and non-specific immunity of crayfish.•Dietary trehalose increased muscle crude protein content and reduced lipid content.•Dietary trehalose increased trehalose content in hepatopancreas and intestine.•Supplementation of 5–15 g kg−1 trehalose improved the resistance to desiccation.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
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