O artigo discute a poesia de Sor Juana Inés de la Cruz, poetiza monja mexicana que, a partir de uma geografia vulcânica, montanhosa e engastada entre esses dois grandes oceanos, tornou poesia não ...apenas esta geografia, como situação, mas também a história atormentada, sangrenta e milenar do planalto de Anauc. Representante da poesia barroca, seus versos são repletos de imagens do cosmo, com imagens noturnas, da pirâmide projetando a terra sobre a lua e as remotas estrelas. História e geografia se entrelaçam em seu retrato espiritual por espaços alegres, por sua experiência de um mundo e pela busca do saber.
Cocaine is a psychostimulant drug with high addictive proprieties. Evidence suggests that cocaine use leads to critical changes in the immune system, with significant effects on T, B, and natural ...killer (NK) cells and influencing peripheral levels of cytokines. The presence of abstinence-related symptoms during detoxification treatment is known to influence the prognosis. Here, our aim was to investigate immune profiles in women with cocaine use disorder (CUD) according to withdrawal symptoms severity.
Blood samples and clinical data were collected at onset of detoxification treatment of 50 women with CUD. The patients were stratified according to Cocaine Selective Severity Assessment (CSSA) scores in low withdrawal (L-W) and high withdrawal (H-W) categories. In addition, we also included a control group with 19 healthy women as reference to immune parameters. Peripheral blood was collected and lymphocyte subsets were phenotyped by multi-color flow cytometry (B cells, CD4
T, CD8
T, NK cells, and different stages of T-cell differentiation). PBMCs from patients and healthy controls were stimulated
with phytohemagglutinin (1%) for 72 h to assess the production of Th1/Th2/Th17 cytokines.
Following stimulation, lymphocytes from women with CUD produced increased levels of Th1/Th2/Th17 cytokines. However, higher levels of IL-2 and IL-17 were observed only in the L-W group, while higher levels of IL-6 were detected in the H-W group compared to controls. H-W group showed lower percentage of early-differentiated Th cells (CD4
CD27
CD28
), elevated percentage of Th cells (CD3
CD4
), intermediate-differentiated Th cells (CD4
CD27
CD28
), and B cells (CD3
CD19
). Both CUD groups showed decreased percentages of naïve T cells (CD3
CD4
CD45RA
and CD3
CD8
CD45RA
).
Our data demonstrated that CUD can lead to increased production of Th1/Th2/Th17 cytokines and lymphocyte changes.
We investigate extraintestinal pathogenic genes (ExPEC) related to virulence of Escherichia coli in flies from the dairy environment.
We collected 217 flies from nine dairy farms, which were ...submitted to microbiological culture. Fifty-one E. coli were identified using mass spectrometry. Eleven dipteran families were identified, with a predominance of Muscidae, and a minor frequency of Tachinidae, Drosophilidae, Sphaeroceridae, Ulidiidae, Syrphidae, Chloropidae, Calliphoridae, Sarcophagidae, and Piophilidae. A panel of 16 virulence-encoding genes related to ExPEC infections were investigated, which revealed predominance of serum resistance (traT, 31/51 = 60.8%; ompT, 29/51 = 56.9%), iron uptake (irp2, 17/51 = 33.3%, iucD 11/51 = 21.6%), and adhesins (papC, 6/51 = 11.8%; papA, 5/51 = 9.8%).
Our findings reveal Dipterans from milking environment carrying ExPEC virulence-encoding genes also identified in clinical bovine E. coli-induced infections.
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BFBNIB, FZAB, GIS, IJS, KILJ, OILJ, SBCE, SBMB, UPUK
Ants, an ecologically successful and numerically dominant group of animals, play key ecological roles as soil engineers, predators, nutrient recyclers, and regulators of plant growth and reproduction ...in most terrestrial ecosystems. Further, ants are widely used as bioindicators of the ecological impact of land use. We gathered information of ant species in the Atlantic Forest of South America. The ATLANTIC ANTS data set, which is part of the ATLANTIC SERIES data papers, is a compilation of ant records from collections (18,713 records), unpublished data (29,651 records), and published sources (106,910 records; 1,059 references), including papers, theses, dissertations, and book chapters published from 1886 to 2020. In total, the data set contains 153,818 ant records from 7,636 study locations in the Atlantic Forest, representing 10 subfamilies, 99 genera, 1,114 ant species identified with updated taxonomic certainty, and 2,235 morphospecies codes. Our data set reflects the heterogeneity in ant records, which include ants sampled at the beginning of the taxonomic history of myrmecology (the 19th and 20th centuries) and more recent ant surveys designed to address specific questions in ecology and biology. The data set can be used by researchers to develop strategies to deal with different macroecological and region‐wide questions, focusing on assemblages, species occurrences, and distribution patterns. Furthermore, the data can be used to assess the consequences of changes in land use in the Atlantic Forest on different ecological processes. No copyright restrictions apply to the use of this data set, but we request that authors cite this data paper when using these data in publications or teaching events.
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BFBNIB, FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SAZU, SBCE, SBMB, UL, UM, UPUK
Leishmaniasis is a highly prevalent, yet neglected disease caused by protozoan parasites of the genus Leishmania. In the search for newer, safer, and more effective antileishmanial compounds, we ...herein present a study of the mode of action in addition to a detailed structural and biological characterization of LQOF-G6 N-benzoyl-N′-benzyl-N″-(4-tertbutylphenyl)guanidine. X-ray crystallography and extensive NMR experiments revealed that LQOF-G6 nearly exclusively adopts the Z conformation stabilized by an intramolecular hydrogen bond. The investigated guanidine showed selective inhibitory activity on Leishmania major cysteine protease LmCPB2.8ΔCTE (CPB) with ~73% inhibition and an IC50-CPB of 6.0 µM. This compound did not show any activity against the mammalian homologues cathepsin L and B. LQOF-G6 has been found to be nontoxic toward both organs and several cell lines, and no signs of hepatotoxicity or nephrotoxicity were observed from the analysis of biochemical clinical plasma markers in the treated mice. Docking simulations and experimental NMR measurements showed a clear contribution of the conformational parameters to the strength of the binding in the active site of the enzyme, and thus fit the differences in the inhibition values of LQOF-G6 compared to the other guanidines. Furthermore, the resulting data render LQOF-G6 suitable for further development as an antileishmanial drug.
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IZUM, KILJ, NUK, PILJ, PNG, SAZU, UL, UM, UPUK
It is known that antidiabetic drug metformin, which is used worldwide, has anti-cancer effects and can be used to prevent cancer growth. We tested the hypothesis that tumor cell growth can be ...inhibited by early treatment with metformin. For this purpose, adult rats chronically treated with metformin in adolescence or in adulthood were inoculated with Walker 256 carcinoma cells. Adult rats that were treated with metformin during adolescence presented inhibition of tumor growth, and animals that were treated during adult life did not demonstrate any changes in tumor growth. Although we do not have data to disclose a molecular mechanism to the preventive metformin effect, we present, for the first time, results showing that cancer growth in adult life is dependent on early life intervention, thus supporting a new therapeutic prevention for cancer.
Leishmaniasis is a highly prevalent, yet neglected disease caused by protozoan parasites of the genus Leishmania. In the search for newer, safer, and more effective antileishmanial compounds, we ...herein present a study of the mode of action in addition to a detailed structural and biological characterization of LQOF-G6 N-benzoyl-N′-benzyl-N″-(4-tertbutylphenyl)guanidine. X-ray crystallography and extensive NMR experiments revealed that LQOF-G6 nearly exclusively adopts the Z conformation stabilized by an intramolecular hydrogen bond. The investigated guanidine showed selective inhibitory activity on Leishmania major cysteine protease LmCPB2.8ΔCTE (CPB) with ~73% inhibition and an ICsub.50-CPB of 6.0 µM. This compound did not show any activity against the mammalian homologues cathepsin L and B. LQOF-G6 has been found to be nontoxic toward both organs and several cell lines, and no signs of hepatotoxicity or nephrotoxicity were observed from the analysis of biochemical clinical plasma markers in the treated mice. Docking simulations and experimental NMR measurements showed a clear contribution of the conformational parameters to the strength of the binding in the active site of the enzyme, and thus fit the differences in the inhibition values of LQOF-G6 compared to the other guanidines. Furthermore, the resulting data render LQOF-G6 suitable for further development as an antileishmanial drug.
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IZUM, KILJ, NUK, PILJ, PNG, SAZU, UL, UM, UPUK
Leishmaniasis is a highly prevalent, yet neglected disease caused by protozoan parasites of the genus
. In the search for newer, safer, and more effective antileishmanial compounds, we herein present ...a study of the mode of action in addition to a detailed structural and biological characterization of
-benzoyl-
'-benzyl-
″-(4-tertbutylphenyl)guanidine. X-ray crystallography and extensive NMR experiments revealed that
nearly exclusively adopts the
conformation stabilized by an intramolecular hydrogen bond. The investigated guanidine showed selective inhibitory activity on
cysteine protease
CPB2.8ΔCTE (CPB) with ~73% inhibition and an IC
of 6.0 µM. This compound did not show any activity against the mammalian homologues cathepsin L and B.
has been found to be nontoxic toward both organs and several cell lines, and no signs of hepatotoxicity or nephrotoxicity were observed from the analysis of biochemical clinical plasma markers in the treated mice. Docking simulations and experimental NMR measurements showed a clear contribution of the conformational parameters to the strength of the binding in the active site of the enzyme, and thus fit the differences in the inhibition values of
compared to the other guanidines. Furthermore, the resulting data render
suitable for further development as an antileishmanial drug.
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IZUM, KILJ, NUK, PILJ, PNG, SAZU, UL, UM, UPUK
Introdução: O artigo tem como objetivo avaliar o uso de fármacos GLP1 no tratamento de diabetes tipo 1. Serão apresentados conceitos relacionados à diabetes tipo 1 e como os análogos de GLP1 vêm ...sendo indicados em estudos recentes. Objetivos: O objetivo deste trabalho é analisar a eficácia e a segurança dos análogos de GLP1 no controle glicêmico em pacientes com diabetes tipo 1, bem como discutir as recomendações clínicas para o seu uso e as perspectivas futuras da terapia combinada com insulina. Metodologia: Serão apresentados estudos clínicos que avaliaram a eficácia e a segurança dos análogos de GLP1 no tratamento de diabetes tipo 1. Serão discutidos os resultados, efeitos colaterais, recomendações clínicas e possíveis interações medicamentosas desses fármacos. Resultados: Os estudos apresentados demonstram a eficácia dos análogos de GLP1 no controle glicêmico, diminuição da dosagem de insulina necessária, diminuição da hipoglicemia e melhora no perfil lipídico dos pacientes com diabetes tipo 1. Também serão abordados os efeitos colaterais desses fármacos e como eles foram controlados nos estudos avaliados. Discussão: Serão discutidas as recomendações clínicas para o uso de análogos de GLP1 em pacientes com diabetes tipo 1, bem como as possíveis interações medicamentosas e contraindicações. Além disso, serão apresentadas perspectivas futuras de terapia combinada com insulina e a necessidade de mais estudos que avaliem a segurança e eficácia desses fármacos a longo prazo em pacientes com diabetes tipo 1. Conclusão: O uso de análogos de GLP1 pode ser uma opção eficaz no tratamento de pacientes com diabetes tipo 1. A terapia combinada com insulina pode evoluir em um futuro próximo, possibilitando tratamentos personalizados para pacientes com essa patologia. No entanto, é necessário realizar mais estudos para avaliar a segurança e eficácia desses medicamentos a longo prazo.
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