In this study, a new species
belonging to the
species complex is established to accommodate an endophytic fungus isolated from
sp. and collected from Himachal Pradesh. The identity of this isolate ...was confirmed based on the asexual morphs, its cultural characteristics, and phylogenetic analyses. This isolate revealed out to be distinct by showing less similarity with described species in the genus
based on molecular sequence data, approximately 93.9% similarity based on translation elongation factor 1-alpha, and 94.2% similarity based on RNA polymerase II subunit. Furthermore, to increase knowledge about this novel species, whole-genome sequencing was carried out. The results displayed that
NFCCI 5145 possesses a 40.2 Mb genome and 48.39% of GC content. Approximately 12,963 functional protein-coding genes were carefully predicted and annotated using different BLAST databases, such as Uniprot, Kyoto Encyclopedia of Genes and Genomes (KEGG), Gene Ontology (GO), Pathogen Host Interactions (PHI), Clusters of Orthologous Groups (COG), and Carbohydrate-Active enzymes (CAZy). The orthologous proteins were identified using OrthoFinder and used for the phylogenetic analysis. ANIb confirmed that the isolate is closely related to the
species complex. It is known that
strains can produce a wide range of bioactive secondary metabolites. Therefore, in-depth mining for biosynthetic gene clusters for secondary metabolite biosynthesis of
NFCCI 5145 was investigated using Antibiotics and Secondary Metabolites Analysis Shell (AntiSMASH) annotation. AntiSMASH results displayed that this isolate possesses 45 secondary metabolites of biosynthetic gene clusters (BGCs). These findings significantly improved our understanding of the strain
NFCCI 5145 and its possible applications in different sectors including industry for the secondary metabolites and enzymes it can produce.
The taxonomy of the genus Fusarium has been in a flux because of ambiguous circumscription of species-level identification based on morphotaxonomic criteria. In this study, multigene phylogeny was ...conducted to resolve the evolutionary relationships of 88 Indian Fusarium isolates based on the internal transcribed spacer region, 28S large subunit, translation elongation factor 1-alpha, RNA polymerase second largest subunit, beta-tubulin and calmodulin gene regions. Fusarium species are well known to produce metabolites such as beauvericin (BEA) and enniatins. These identified isolates were subjected to fermentation in Fusarium-defined media for BEA production and tested using TLC, HPLC and HRMS. Among 88 isolates studied, 50 were capable of producing BEA, which varied from 0.01 to 15.82 mg/g of biomass. Fusarium tardicrescens NFCCI 5201 showed maximum BEA production (15.82 mg/g of biomass). The extract of F. tardicrescens NFCCI 5201 showed promising antibacterial activity against Staphylococcus aureus MLS16 MTCC 2940 and Micrococcus luteus MTCC 2470 with MIC of 62.5 and 15.63 µg/mL, respectively. Similarly, the F. tardicrescens NFCCI 5201 extract in potato dextrose agar (40 µg/mL) exhibited antifungal activity in the food poison technique against plant pathogenic and other fungi, Rhizoctonia solani NFCCI 4327, Sclerotium rolfsii NFCCI 4263, Geotrichum candidum NFCCI 3744 and Pythium sp. NFCCI 3482, showing % inhibition of 84.31, 49.76, 38.22 and 35.13, respectively. The antibiotic effect was found to synergize when Fusarium extract and amphotericin B (20 µg/mL each in potato dextrose agar) were used in combination against Rhizopus sp. NFCCI 2108, Sclerotium rolfsii NFCCI 4263, Bipolaris sorokiniana NFCCI 4690 and Absidia sp. NFCCI 2716, showing % inhibition of 50.35, 79.37, 48.07 and 76.72, respectively. The extract also showed satisfactory dose-dependent DPPH radical scavenging activity with an IC50 value of 0.675 mg/mL. This study reveals the correct identity of the Indian Fusarium isolates based on multigene phylogeny and also throws light on BEA production potential, suggesting their possible applicability in the medicine, agriculture and industry.
As the continuation of Fungal Diversity Notes series, the current paper is the 16th contribution to this series. A total of 103 taxa from seven classes in
Ascomycota
and
Basidiomycota
are included ...here. Of these 101 taxa, four new genera, 89 new species, one new combination, one new name and six new records are described in detail along with information of hosts and geographic distributions. The four genera newly introduced are
Ascoglobospora
,
Atheliella
,
Rufoboletus
and
Tenuimyces
. Newly described species are
Akanthomyces xixiuensis
,
Agaricus agharkarii
,
A. albostipitatus
,
Amphisphaeria guttulata
,
Ascoglobospora marina
,
Astrothelium peudostraminicolor
,
Athelia naviculispora
,
Atheliella conifericola
,
Athelopsis
subglaucina
,
Aureoboletus minimus
,
A. nanlingensis
,
Autophagomyces incertus
,
Beltrania liliiferae
,
Beltraniella jiangxiensis
,
Botryobasidium coniferarum
,
Calocybella sribuabanensis
,
Calonarius caesiofulvus
,
C. nobilis
,
C. pacificus
,
C. pulcher
,
C. subcorrosus
,
Cortinarius flaureifolius
,
C. floridaensis
,
C. subiodes
,
Crustomyces juniperi
,
C. scytinostromoides
,
Cystostereum subsirmaurense
,
Dimorphomyces seemanii
,
Fulvoderma microporum
,
Ginnsia laricicola
,
Gomphus zamorinorum
,
Halobyssothecium sichuanense
,
Hemileccinum duriusculum
,
Henningsomyces hengduanensis
,
Hygronarius californicus
,
Kneiffiella pseudoabdita
,
K. pseudoalutacea
,
Laboulbenia bifida
,
L. tschirnhausii
,
L. tuberculata
,
Lambertella dipterocarpacearum
,
Laxitextum subrubrum
,
Lyomyces austro-occidentalis
,
L. crystallina
,
L. guttulatus
,
L. niveus
,
L. tasmanicus
,
Marasmius centrocinnamomeus
,
M. ferrugineodiscus
,
Megasporoporia tamilnaduensis
,
Meruliopsis crystallina
,
Metuloidea imbricata
,
Moniliophthora atlantica
,
Mystinarius ochrobrunneus
,
Neomycoleptodiscus alishanense
,
Nigrograna kunmingensis
,
Paracremonium aquaticum
,
Parahelicomyces dictyosporus
,
Peniophorella sidera
,
P. subreticulata
,
Phlegmacium fennicum
,
P. pallidocaeruleum
,
Pholiota betulicola
,
P. subcaespitosa
,
Pleurotheciella hyalospora
,
Pleurothecium aseptatum
,
Resupinatus porrigens
,
Russula chlorina
,
R. chrysea
,
R. cruenta
,
R. haematina
,
R. luteocarpa
,
R. sanguinolenta
,
Synnemellisia punensis
,
Tenuimyces bambusicola
,
Thaxterogaster americanoporphyropus
,
T. obscurovibratilis
,
Thermoascus endophyticus
,
Trechispora alba
,
T. perminispora
,
T. subfarinacea
,
T. tuberculata
,
Tremella sairandhriana
,
Tropicoporus natarajaniae
,
T. subramaniae
,
Usnea kriegeriana
,
Wolfiporiella macrospora
and
Xylodon muchuanensis
.
Rufoboletus hainanensis
is newly transferred from
Butyriboletus
, while a new name
Russula albocarpa
is proposed for
Russula leucocarpa
G.J. Li & Chun Y. Deng an illegitimate later homonym of
Russula leucocarpa
(T. Lebel) T. Lebel. The new geographic distribution regions are recorded for
Agaricus bambusetorum
,
Bipolaris heliconiae
,
Crinipellis trichialis
,
Leucocoprinus cretaceus
,
Halobyssothecium cangshanense
and
Parasola setulosa
. Corresponding to morphological characters, phylogenetic evidence is also utilized to place the above-mentioned taxa in appropriate taxonomic positions. The current morphological and phylogenetic data is helpful for further clarification of species diversity and exploration of evolutionary relationships in the related fungal groups.
Full text
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EMUNI, FIS, FZAB, GEOZS, GIS, IJS, IMTLJ, KILJ, KISLJ, MFDPS, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
Fungal diversity notes is one of the important journal series of fungal taxonomy that provide detailed descriptions and illustrations of new fungal taxa, as well as providing new information of ...fungal taxa worldwide. This article is the 11th contribution to the fungal diversity notes series, in which 126 taxa distributed in two phyla, six classes, 24 orders and 55 families are described and illustrated. Taxa in this study were mainly collected from Italy by Erio Camporesi and also collected from China, India and Thailand, as well as in some other European, North American and South American countries. Taxa described in the present study include two new families, 12 new genera, 82 new species, five new combinations and 25 new records on new hosts and new geographical distributions as well as sexual-asexual reports. The two new families are
Eriomycetaceae
(Dothideomycetes, family
incertae sedis
) and
Fasciatisporaceae
(
Xylariales
, Sordariomycetes). The twelve new genera comprise
Bhagirathimyces
(
Phaeosphaeriaceae
),
Camporesiomyces
(
Tubeufiaceae
),
Eriocamporesia
(
Cryphonectriaceae
),
Eriomyces
(
Eriomycetaceae
),
Neomonodictys
(
Pleurotheciaceae
),
Paraloratospora
(
Phaeosphaeriaceae
),
Paramonodictys
(
Parabambusicolaceae
),
Pseudoconlarium
(Diaporthomycetidae, genus
incertae sedis
),
Pseudomurilentithecium
(
Lentitheciaceae
),
Setoapiospora
(
Muyocopronaceae
),
Srinivasanomyces
(
Vibrisseaceae
) and
Xenoanthostomella
(
Xylariales
, genera
incertae sedis
). The 82 new species comprise
Acremonium chiangraiense
,
Adustochaete nivea
,
Angustimassarina camporesii
,
Bhagirathimyces himalayensis
,
Brunneoclavispora camporesii
,
Camarosporidiella camporesii
,
Camporesiomyces mali
,
Camposporium appendiculatum
,
Camposporium multiseptatum
,
Camposporium septatum
,
Canalisporium aquaticium
,
Clonostachys eriocamporesiana
,
Clonostachys eriocamporesii
,
Colletotrichum hederiicola
,
Coniochaeta vineae
,
Conioscypha verrucosa
,
Cortinarius ainsworthii
,
Cortinarius aurae
,
Cortinarius britannicus
,
Cortinarius heatherae
,
Cortinarius scoticus
,
Cortinarius subsaniosus
,
Cytospora fusispora
,
Cytospora rosigena
,
Diaporthe camporesii
,
Diaporthe nigra
,
Diatrypella yunnanensis
,
Dictyosporium muriformis
,
Didymella camporesii
,
Diutina bernali
,
Diutina sipiczkii
,
Eriocamporesia aurantia
,
Eriomyces heveae
,
Ernakulamia tanakae
,
Falciformispora uttaraditensis
,
Fasciatispora cocoes
,
Foliophoma camporesii
,
Fuscostagonospora camporesii
,
Helvella subtinta
,
Kalmusia erioi
,
Keissleriella camporesiana
,
Keissleriella camporesii
,
Lanspora cylindrospora
,
Loratospora arezzoensis
,
Mariannaea atlantica
,
Melanographium phoenicis
,
Montagnula camporesii
,
Neodidymelliopsis camporesii
,
Neokalmusia kunmingensis
,
Neoleptosporella camporesiana
,
Neomonodictys muriformis
,
Neomyrmecridium guizhouense
,
Neosetophoma camporesii
,
Paraloratospora camporesii
,
Paramonodictys solitarius
,
Periconia palmicola
,
Plenodomus triseptatus
,
Pseudocamarosporium camporesii
,
Pseudocercospora maetaengensis
,
Pseudochaetosphaeronema kunmingense
,
Pseudoconlarium punctiforme
,
Pseudodactylaria camporesiana
,
Pseudomurilentithecium camporesii
,
Pseudotetraploa rajmachiensis
,
Pseudotruncatella camporesii
,
Rhexocercosporidium senecionis
,
Rhytidhysteron camporesii
,
Rhytidhysteron erioi
,
Septoriella camporesii
,
Setoapiospora thailandica
,
Srinivasanomyces kangrensis
,
Tetraploa dwibahubeeja
,
Tetraploa pseudoaristata
,
Tetraploa thrayabahubeeja
,
Torula camporesii
,
Tremateia camporesii
,
Tremateia lamiacearum
,
Uzbekistanica pruni
,
Verruconis mangrovei
,
Wilcoxina verruculosa
,
Xenoanthostomella chromolaenae
and
Xenodidymella camporesii
. The five new combinations are
Camporesiomyces patagoniensis
,
Camporesiomyces vaccinia
,
Camposporium lycopodiellae
,
Paraloratospora gahniae
and
Rhexocercosporidium microsporum
. The 22 new records on host and geographical distribution comprise
Arthrinium marii
,
Ascochyta medicaginicola
,
Ascochyta pisi
,
Astrocystis bambusicola
,
Camposporium pellucidum
,
Dendryphiella phitsanulokensis
,
Diaporthe foeniculina
,
Didymella macrostoma
,
Diplodia mutila
,
Diplodia seriata
,
Heterosphaeria patella
,
Hysterobrevium constrictum
,
Neodidymelliopsis ranunculi
,
Neovaginatispora fuckelii
,
Nothophoma quercina
,
Occultibambusa bambusae
,
Phaeosphaeria chinensis
,
Pseudopestalotiopsis theae
,
Pyxine berteriana
,
Tetraploa sasicola
,
Torula gaodangensis
and
Wojnowiciella dactylidis
. In addition, the sexual morphs of
Dissoconium eucalypti
and
Phaeosphaeriopsis pseudoagavacearum
are reported from
Laurus nobilis
and
Yucca gloriosa
in Italy, respectively. The holomorph of
Diaporthe cynaroidis
is also reported for the first time.
Full text
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EMUNI, FIS, FZAB, GEOZS, GIS, IJS, IMTLJ, KILJ, KISLJ, MFDPS, NLZOH, NUK, OBVAL, OILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
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