Curcumin, a major component of turmeric, is known to exhibit multiple biological functions including antitumor activity. We previously reported that the mitogen-activated protein kinase (MAPK) ...scaffold protein c-Jun NH2-terminal kinase (JNK)-associated leucine zipper protein (JLP) reduces curcumin-induced cell death by modulating p38 MAPK and autophagy through the regulation of lysosome positioning. In this study, we investigated the role of JNK/stress-activated protein kinase-associated protein 1 (JSAP1), a JLP family member, in curcumin-induced stress, and found that JSAP1 also attenuates curcumin-induced cell death. However, JSAP1 knockout showed no or little effect on the activation of JNK and p38 MAPKs in response to curcumin. In addition, small molecule inhibitors of JNK and p38 MAPKs did not increase curcumin-induced cell death. Furthermore, JSAP1 depletion did not impair lysosome positioning and autophagosome-lysosome fusion. Instead, we noticed substantial autolysosome accumulation accompanied by an inefficient autophagic flux in JSAP1 knockout cells. Taken together, these results indicate that JSAP1 is involved in curcumin-induced cell death differently from JLP, and may suggest that JSAP1 plays a role in autophagosome degradation and its dysfunction results in enhanced cell death. The findings of this study may contribute to the development of novel therapeutic approaches using curcumin for cancer.
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IZUM, KILJ, NUK, PILJ, PNG, SAZU, UL, UM, UPUK
The temperature characteristics of carbon spectator fragments formed in carbon collisions with carbon nuclei at a primary momentum of 4.2 GeV/c per nucleon were presented and discussed on corrected ...experimental data. As well as studied the multiplicities formed by the spectator protons, deuterons, and tritons in the inelastic nucleus-nucleus interactions. We found that the temperature absorbed by the spectator fragments is dependent on their mass.
Lysosomes are involved in many cellular functions, and in turn lysosomal dysfunction underlies a variety of diseases, including cancer and neurodegenerative diseases. Lysosomes are distributed ...broadly in the cytoplasm and can move throughout the cell in kinesin- and dynein-dependent manners. Although many mechanisms of lysosomal transport have been reported, how lysosomal transport is regulated has yet to be fully elucidated. In this study we analyzed c-Jun NH
-terminal kinase-associated leucine zipper protein (JLP), an adaptor of kinesin and dynein motor proteins, and found that lysosomes were localized toward the cell periphery in JLP knockdown cells, leading to the impairment of autophagosome-lysosome fusion. Furthermore, we performed rescue experiments using wild-type JLP and its various deletion mutants. The results indicated that JLP may regulate lysosome localization and autophagy through interaction of JLP with kinesin-1 heavy chain, but not with dynactin p150
or lysosomal transmembrane protein 55b. Our findings provide new insights into the mechanisms of lysosomal trafficking regulation. This study contributes to the understanding of how lysosomes exert their multiple functions, potentially leading to the identification of molecular targets for diseases caused by lysosomal dysfunction.
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IZUM, KILJ, NUK, PILJ, PNG, SAZU, UL, UM, UPUK
碩士
崑山科技大學
機械工程研究所
107
The procedure of Reverse Engineering (RE) is provided in this thesis. The associated techniques such as physical part scanning and surface recreation are explained in great ...detail. Whole scale Reverse Engineering were conducted for four real pieces: A computer mouse, a telephone handset, a spray bottle and a bicycle seat frame.
From Prepare the real piece for scan, scan into point cloud with a laser scanner, open the scan file in RE software, editing the original data, create corresponding curves from the model, create surfaces, edit, stitch and complete the surface model, convert into 3D solid model, export to the format other software can open, until use other CAD software to verify the availability of the CAD file recreated through the procedure. Four 3D CAD models were created successfully within acceptable accuracy.
After finished the reverse, change to materials and get a comparison result from simulation. Comparison of carbon fiber VS steel. Results is carbon fiber softer and stronger than steel.
Moreover, from the 3D model obtained with RE, Computer Aided Engineering analysis was conducted for one of the piece: the bicycle seat frame. The advantage of Carbon Fiber Reinforced Plastic material was investigated compared to conventional stainless steel part. The results showed the displacement with the use of CFRP is 4 times less, the factor of safety is 5 times better, and the weight is approximately 7 times less in comparison to the ones with conventional stainless steel. It’s apparent that with the application of reverse engineering on the existed product, the improvements of that product is much easier and faster which is very suitable for the industry.
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