Existing high-throughput methods to identify RNA-binding proteins (RBPs) are based on capture of polyadenylated RNAs and cannot recover proteins that interact with nonadenylated RNAs, including long ...noncoding RNA, pre-mRNAs and bacterial RNAs. We present orthogonal organic phase separation (OOPS), which does not require molecular tagging or capture of polyadenylated RNA, and apply it to recover cross-linked protein-RNA and free protein, or protein-bound RNA and free RNA, in an unbiased way. We validated OOPS in HEK293, U2OS and MCF10A human cell lines, and show that 96% of proteins recovered were bound to RNA. We show that all long RNAs can be cross-linked to proteins, and recovered 1,838 RBPs, including 926 putative novel RBPs. OOPS is approximately 100-fold more efficient than existing methods and can enable analyses of dynamic RNA-protein interactions. We also characterize dynamic changes in RNA-protein interactions in mammalian cells following nocodazole arrest, and present a bacterial RNA-interactome for Escherichia coli. OOPS is compatible with downstream proteomics and RNA sequencing, and can be applied in any organism.
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EMUNI, FIS, FZAB, GEOZS, GIS, IJS, IMTLJ, KILJ, KISLJ, MFDPS, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
Insects have been the subject of recent attention as a potentially environmentally sustainable and nutritious alternative to traditional protein sources. The purpose of this paper is to test the ...hypothesis that insects are nutritionally preferable to meat, using two evaluative tools that are designed to combat over- and under-nutrition.
We selected 183 datalines of publicly available data on the nutrient composition of raw cuts and offal of three commonly consumed meats (beef, pork and chicken), and six commercially available insect species, for energy and 12 relevant nutrients. We applied two nutrient profiling tools to this data: The Ofcom model, which is used in the United Kingdom, and the Nutrient Value Score (NVS), which has been used in East Africa. We compared the median nutrient profile scores of different insect species and meat types using non-parametric tests and applied Bonferroni adjustments to assess for statistical significance in differences.
Insect nutritional composition showed high diversity between species. According to the Ofcom model, no insects were significantly 'healthier' than meat products. The NVS assigned crickets, palm weevil larvae and mealworm a significantly healthier score than beef (P<0.001) and chicken (P<0.001). No insects were statistically less healthy than meat.
Insect nutritional composition is highly diverse in comparison with commonly consumed meats. The food category 'insects' contains some foods that could potentially exacerbate diet-related public health problems related to over-nutrition, but may be effective in combating under-nutrition.
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DOBA, EMUNI, FIS, FZAB, GEOZS, GIS, IJS, IMTLJ, IZUM, KILJ, KISLJ, MFDPS, NLZOH, NUK, OILJ, PILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, SIK, UILJ, UKNU, UL, UM, UPUK, VKSCE, VSZLJ, ZAGLJ
•Potassium release from combusting biomass studied with flame emission spectroscopy.•Biomass ash with differing quantities of an additive investigated.•3 different biomass fuels examined; softwood, ...wheat straw and olive residue.•The additive decreased K-release profiles for all biomass.•Softwood shows a high K-release rate relative to the small quantity of K in this fuel.
This study focuses on the effects of an aluminosilicate additive on the fate of potassium during biomass combustion. Such additives have shown some success in mitigating slagging and fouling problems in boilers and furnaces, and the mobility of potassium in combustion systems is one of the key factors dictating ash behaviour. To investigate this, a flame emission spectroscopy technique was used to evaluate the differences in the gas-phase potassium release profiles during the combustion of 5 mm diameter pellets of different biomass suspended in a methane-air flame. The biomass pellets were evaluated with various mixes of an aluminosilicate based additive (5, 15 and 25 wt%). Potassium emission detection, coupled with high speed video of the combustion process, indicated that potassium evolves over the three stages of volatile combustion (a sharp peak in the emission profile), char combustion (a broader peak) and “ash cooking” (a very broad peak over an extended period, long after the end of combustion). In the absence of additive, the three biomass studied (softwood, wheat straw, olive residue) behaved quite differently in terms of potassium release profiles. When the results are normalized for the amount of potassium in the fuel, it is clear that a large fraction of potassium enters the gas phase during the volatile and char combustion of the softwood. Olive residue, releases a lower fraction of potassium during the volatile and char combustion stages, indicating that more potassium is fixed in the ash. In contrast, wheat straw shows a release of potassium during combustion, and then, after a period of “ash cooking”, a substantial gradual release with continued exposure to hot combustion gases. The difference in the emission profiles can be interpreted in terms of the K:Cl ratios and the K:(Si + Al) ratios: high chlorine and/or low (Si + Al) facilitates the release of KCl or KOH to the gas phase, while high (Si + Al) helps to fix K in the solid phase. The addition of the aluminosilicate additive shows a clear reduction in the potassium released from all the biomass pellets, particularly during the char-oxidation and “ash cooking” stages, and the level of additive required is related to the amount of K in the biomass. The potassium emission experiments were complemented by laboratory-scale preparation of ash at different temperatures, and detection of residual potassium in the ash using Atomic Absorption Spectroscopy (AAS). These results validated the findings and quantified the higher fractions of potassium retained within the ash when additives are used. For the wood ash 70–100% of K is retained in the ash in the presence of additive; for the wheat straw, this figure is 60–80% and for the olive pellets it is 70–100%.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK, ZRSKP
•Small granular starches from rice, quinoa and amaranth were hydrophobized by OSA.•Starch granules from small granule botanical sources have emulsification capacity.•Quinoa and rice starch granules ...have emulsifying capacity in native and modified form.•Emulsifying properties depend on modification level, size and concentration of starch.•Quinoa starch granules had the best emulsifying capacity followed by amaranth and rice.
Small granular starches from rice, quinoa and amaranth were hydrophobized by esterification with octenyl succinic anhydride (OSA) in an aqueous alkaline slurry to obtain series of modified starches at defined intervals (i.e. 0.6, 1.2, 1.8, 2.4, 3.0%). The physical and the physico-chemical properties of the starch particles were characterized by proximate analysis including protein level, amylose level and dry matter. The shape and size of the starch granules were characterized by scanning electron microscopy and light scattering. The gelatinization properties were characterized by differential scanning calorimetry. The degree of modification was determined by titration with NaOH. With regard to the emulsion formulation and in order to assess the emulsifying capacity of the small granular starches, the effect of starch type, degree of modification and starch concentration on the resulting emulsion droplet size were evaluated by light scattering and optical microscopy.
Emulsifying properties were found to depend on the degree of substitution, size of the granules and the starch to oil ratio of the formulation. Quinoa starch granules, in general, had the best emulsifying capacity followed by amaranth and rice. However, in higher starch concentrations (>400mg/mL oil) and adequate levels of OSA (3.0%) amaranth performed best, having the smallest size of starches studied.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK, ZRSKP
RNA-protein interactions play a pivotal role in cell homeostasis and disease, but current approaches to study them require a considerable amount of starting material, favor the recovery of only a ...subset of RNA species or are complex and time-consuming. We recently developed orthogonal organic phase separation (OOPS): a quick, efficient and reproducible method to purify cross-linked RNA-protein adducts in an unbiased way. OOPS avoids molecular tagging or the capture of polyadenylated RNA. Instead, it is based on sampling the interface of a standard TRIzol extraction to enrich RNA-binding proteins (RBPs) and their cognate bound RNA. OOPS specificity is achieved by digesting the enriched interfaces with RNases or proteases to release the RBPs or protein-bound RNA, respectively. Here we present a step-by-step protocol to purify protein-RNA adducts, free protein and free RNA from the same sample. We further describe how OOPS can be applied in human cell lines, Arabidopsis thaliana, Schizosaccharomyces pombe and Escherichia coli and how it can be used to study RBP dynamics.
The consumption of free sugars in the UK is more than double the guideline intake for adults and close to triple for children, with soft drinks representing a significant proportion. The aim of this ...study was to assess how individual soft drink companies and consumers have responded to calls to reduce sugar consumption, including the soft drink industry levy (SDIL), between 2015 and 2018.
This was an annual cross-sectional study using nutrient composition data of 7377 products collected online, paired with volume sales data for 195 brands offered by 57 companies. The main outcome measures were sales volume, sugar content and volume of sugars sold by company and category, expressed in total and per capita per day terms.
Between 2015 and 2018, the volume of sugars sold per capita per day from soft drinks declined by 30%, equivalent to a reduction of 4.6 g per capita per day. The sales-weighted mean sugar content of soft drinks fell from 4.4 g/100 ml in 2015 to 2.9 g/100 ml in 2018. The total volume sales of soft drinks that are subject to the SDIL (i.e. contain more than 5 g/100 ml of sugar) fell by 50%, while volume sales of low- and zero-sugar (< 5 g/100 ml) drinks rose by 40%.
Action by the soft drinks industry to reduce sugar in products and change their product portfolios, coupled with changes in consumer purchasing, has led to a significant reduction in the total volume and per capita sales of sugars sold in soft drinks in the UK. The rate of change accelerated between 2017 and 2018, which also implies that the implementation of the SDIL acted as an extra incentive for companies to reformulate above and beyond what was already being done as part of voluntary commitments to reformulation, or changes in sales driven by consumer preferences.
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DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
There is much concern about the toxicological effects of synthetic hair dyes. As an alternative approach, renewable waste blackcurrant (Ribes nigrum L.) fruit skins from the fruit pressing industry ...were extracted using acidified water with a solid-phase purification stage. Anthocyanin colorants were isolated in good yields (2–3% w/w) and characterized by HPLC. Sorption of anthocyanins onto hair followed a Freundlich isotherm; anthocyanin–anthocyanin aggregation interactions enabled high buildup on the substrate. Sorption energy of cyanidin-3-O-glucoside (monosaccharide) > cyanidin-3-O-rutinoside (disaccharide), but sorption properties of different anthocyanin glucosides were very similar. Intense blue-colored dyeing on hair could be achieved with λmax‑vis at 580 nm, typical of the anionic quinonoid base; it is suggested that hair provides an environment that enables the stabilization of the anionic quinonoid base on adsorption through association with cations in the hair and copigmentation effects. Dyeings were stable to multiple washes.
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IJS, KILJ, NUK, PNG, UL, UM, UPUK
Although the subcellular dynamics of RNA and proteins are key determinants of cell homeostasis, their characterization is still challenging. Here we present an integrative framework to simultaneously ...interrogate the dynamics of the transcriptome and proteome at subcellular resolution by combining two methods: localization of RNA (LoRNA) and a streamlined density-based localization of proteins by isotope tagging (dLOPIT) to map RNA and protein to organelles (nucleus, endoplasmic reticulum and mitochondria) and membraneless compartments (cytosol, nucleolus and cytosolic granules). Interrogating all RNA subcellular locations at once enables system-wide quantification of the proportional distribution of RNA. We obtain a cell-wide overview of localization dynamics for 31,839 transcripts and 5,314 proteins during the unfolded protein response, revealing that endoplasmic reticulum-localized transcripts are more efficiently recruited to cytosolic granules than cytosolic RNAs, and that the translation initiation factor eIF3d is key to sustaining cytoskeletal function. Overall, we provide the most comprehensive overview so far of RNA and protein subcellular localization dynamics.
An increasing number of countries are introducing taxes on unhealthy food and drinks, but will they improve health? Oliver Mytton, Dushy Clarke, and Mike Rayner examine the evidence
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BFBNIB, CMK, NMLJ, NUK, PNG, SAZU, UL, UM, UPUK
Solar radiation along with other weather variables are commonly processed on typical meteorological years (TMYs) to be applied in the design of various energy systems. However, in several regions of ...the world, solar radiation data usually lacks a suitable and/or representative measurement, which leads to its modeling and prediction to properly fill this information in the databases. Consequently, the accuracy of these models can influence the viability and proper design of such energy systems. Within this context, the present contribution aims to assess the quality of solar radiation data included in the most recent TMY databases with Brazilian data and how that quality can influence the selection of months that create TMYs as well as the building performance simulation (BPS) results. Because two different approaches to generate the solar radiation data are used, we evaluate the global horizontal irradiation data in the two latest versions of recent Brazilian TMY databases against the corresponding satellite-derived ones obtained from the POWER database (NASA). Simultaneously, as another alternative approach, global solar radiation data are calculated for the same studied locations and period through the modeling method used to generate the current version of the International Weather for Energy Calculations (IWEC2), and its performance is also compared against the corresponding reanalysis data (POWER). Finally, a set of case studies applying the local building performance regulations are exhaustively analyzed to quantify the impact of the uncertainty of solar radiation models on BPS results throughout Brazil. The results indicate that the accuracy of solar radiation models can highly influence the resulting TMY configurations. These changes can drive differences up to 40% on the prediction of the ideal annual loads of the residential buildings while, regardless of design performance, differences lower than 10% are found for the commercial case studies in most locations. Conversely, the prediction of peak loads for cooling shows to be more sensitive to the climate data changes in the commercial buildings than in the residential ones.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
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