Summary
Solar radiation regulates most biological activities on Earth. Prolonged exposure to solar UV radiation can cause deleterious effects by inducing two major types of DNA damage, namely, ...cyclobutane pyrimidine dimers (CPDs) and pyrimidine 6‐4 pyrimidone photoproducts. These lesions may be repaired by the photoreactivation (Phr) and nucleotide excision repair (NER) pathways; however, the principal UV‐induced DNA repair pathway is not known in the fungal genus Pseudogymnoascus. In this study, we demonstrated that an unweighted UV‐B dosage of 1.6 kJ m−2 d−1 significantly reduced fungal growth rates (by between 22% and 35%) and inhibited conidia production in a 10 d exposure. The comparison of two DNA repair conditions, light or dark, which respectively induced photoreactivation (Phr) and NER, showed that the UV‐B‐induced CPDs were repaired significantly more rapidly in light than in dark conditions. The expression levels of two DNA repair genes, RAD2 and PHR1 (encoding a protein in NER and Phr respectively), demonstrated that NER rather than Phr was primarily activated for repairing UV‐B‐induced DNA damage in these Pseudogymnoascus strains. In contrast, Phr was inhibited after exposure to UV‐B radiation, suggesting that PHR1 may have other functional roles. We present the first study to examine the capability of the Arctic and Antarctic Pseudogymnoascus sp. to perform photoreactivation and/or NER via RT‐qPCR approaches, and also clarify the effects of light on UV‐B‐induced DNA damage repair in vivo by quantifying cyclobutene pyrimidine dimers and pyrimidine 6‐4 pyrimidone photoproducts. Physiological response data, including relative growth rate, pigmentation and conidia production in these Pseudogymnoascus isolates exposed to UV‐B radiation are also presented.
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BFBNIB, FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SBCE, SBMB, UL, UM, UPUK
Invertebrates are an important source of structurally-diverse and biologically-active halogenated metabolites. The sea hare
Rang has long been known to possess halogenated metabolites of dietary ...origin that are used as a self-defense mechanism. The compounds from
Rang are comprised mainly of terpenoids and small percentages of C-15 acetogenins, indoles, macrolides, sterols and alkaloids with potent cytotoxic, anti-microbial and anti-inflammatory properties. For decades the metabolites discovered have been investigated for their medical and pharmaceutical applications, so much so that the ecological role of the metabolites has been overlooked. The interaction between
Rang and its diet that is comprised of seaweed can provide information into the distribution and diversity of the seaweed, the application of bioaccumulated secondary metabolites as part of its defense mechanism and the potential roles of these metabolites for adaptation in the marine environment. This paper compiles the diversity of halogenated secondary metabolites documented from
Rang.
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IZUM, KILJ, NUK, PILJ, PNG, SAZU, UL, UM, UPUK
This study utilizes criteria and indicators to propose a set of sustainable evaluation methods for ecotourism destinations based on perception. The questionnaires are designed according to the index ...system of sustainable evaluation. Taking Malaysia's Pulau Perhentian and China's Weizhou Island as examples, the method is verified and the coordinated development path of island resource protection and ecotourism in two marine parks is proposed according to the results of sustainability evaluation. In this study, 126 valid questionnaires were gotten from Weizhou Island Marine Park (WZMP) in China and 125 from Pulau Perhentian Marine Park (PPMP) in Malaysia. The outcomes show that PPMP has a higher sustainable development index, and both resource protection index and ecotourism development index are higher than WZMP. To the extent the subtypes of coordinated development are concerned, PPMP is underdeveloped, that is, the broadness and profundity of the tourism industry advancement are insufficient to coordinate its resource level, while WZMP belongs to over-development type. In the decision of development path, WZMP ought to adopt a protection priority mode, while PPMP is suitable for the development priority mode. The effects of this study give valuable information for the planning and management of marine parks and establish a framework for effective management.
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CEKLJ, EMUNI, FIS, FZAB, GEOZS, GIS, IJS, IMTLJ, KILJ, KISLJ, MFDPS, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
This study evaluated the effect of seasonal variation on the physicochemical, biochemical, and nutritional composition of
. Sampling was designed during the main monsoon seasons in Malaysia-the ...Southwest monsoon (SWM) and Northeast monsoon (NEM)-to understand the intraspecific variation (
< 0.05). Carbohydrates, protein, and dietary fiber were found to be higher in NEM-
, whereas a higher ash content was quantified in SWM-
. No significant differences were found in crude lipid and moisture content (
> 0.05). Vitamin B2 was calculated as (0.29 ± 0.06 mg 100 g
) and (0.38 ± 0.06 mg 100 g
) for the NEM and SWM samples, respectively (
< 0.05). The fatty acid profile showed the dominance of saturated fatty acids (SFAs)-palmitic acids, stearic acid, and myristic acid-while the mineral contents were found to be good sources of calcium (1750.97-4047.74 mg 100 g
) and iron (1512.55-1346.05 mg 100 g
). Tryptophan and lysine were recorded as the limiting essential amino acids (EAAs) in NEM
, while leucine and phenylalanine were found to be the limiting EAAs in the SWM samples. None of the extracts exhibited antibacterial properties against the screened strains. The study concluded that seasonal changes have a great effect on the biochemical composition of
.
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IZUM, KILJ, NUK, PILJ, PNG, SAZU, UL, UM, UPUK
Fungal species members of the genus
have been known to occur in marine environments. This report documents the first record of the fungal genus
isolated from scleractinian corals. Three isolated ...strains were identified from a phylogenetic tree that was constructed, based on the nuclear ribosomal internal transcribed spacer and partial large subunit (ITS + LSU) DNA sequences. Isolates were closely related to both
(Crous) Crous and
(Crous, R.G. Shivas & McTaggart) Crous, but formed a distinct clade with strong support that implies a potentially genetic variant of a known species or even a novel species. These findings contribute to the fungal diversity checklist in Malaysia and knowledge about marine fungi associated with scleractinian corals.
Pangasiids are an economically significant group of catfish, and many pangasiids are threatened in the wild from anthropogenic pressures, including increases in fishing pressure, habitat degradation, ...and improperly managed aquaculture practices. This study demonstrates the usage of DNA barcoding of the Cytochrome Oxidase subunit I (COI) gene as an identification tool in detecting potentially threatening invasive pangasiid species by establishing the diversity and phylogenetic relationship of Pangasiidae catfishes in Peninsular Malaysia. A neighbour-joining (NJ) dendrogram (Kimura-2-parameter model) generated five clades to represent distinct genera. Pangasius was further subdivided into two clades (Clade A: Pangasius bocourti-P. djambal and Clade B: P. nasutus-P. conchophilus). Given the marginal genetic divergence, indigenous and non-native species should be treated cautiously in allopatrically distributed species. The analysis used Automatic Barcode Gap Discovery (ABGD) and revealed barcode gaps between the intraspecific and interspecific distances. The sequences were partitioned into five groupings, corresponding with the species delineation based on the distribution of pairwise differences, which could not be differentiated using the NJ dendrogram. ABGD allows the recognition of one or two additional species using the recursive approach, but other taxonomic methods should be considered for a solid conclusion. DNA barcoding demonstrates the identification of closely related species, thus justifying its application towards the conservation of these fish.
Scientific enquiries into jellyfish blooms and associated problems are often deterred by the lack of taxonomical and ecological studies worldwide. Taxonomic difficulty is attributed to the high ...degree of morphological variations among and within species. To date, only two scyphozoan jellyfish species have been documented from field surveys in Malaysian waters, whereas another four Malaysian scyphozoan and two cubozoan jellyfish species have been mentioned in toxicological studies. None of these species have; however, been verified. This study thus aimed to document and resolves the uncertainty of earlier identified species in the region using morphology and molecular DNA sequencing. Jellyfish specimens were collected from Malaysian waters in the Straits of Malacca, South-China Sea and the Sulu-Sulawesi Sea over two years (June 2008 to October 2010), and their DNA sequences were compared with those from the Atlantic and Pacific regions. Ten scyphozoan and two cubozoan species were recorded in Malaysian waters (South China Sea and Straits of Malacca). These jellyfish included eight species from the order Rhizostomeae (Rhizostomatidae, Lobonematidae, Mastigiidae, Catostylidae and Cepheidae), two species from Semaestomeae (Pelagiidae and Cyaneidae) and two species from class Cubozoa; one from order Carybdeida (family Carukiidae) and another from order Chirodropida (family Chiropsalmidae). Molecular identification of species using phylogenetic approaches was based on DNA sequences of partial cytochrome oxidase I (COI), 16S and internal transcribed spacer (ITS1) regions. The COI phylogenetic tree of Cubozoa and Scyphozoa species from the Atlantic and Pacific regions showed distinct clustering of six Malaysian jellyfish species. However, most of the deeper divergences and relationships between the families were unresolved, which were also observed in the 16S and ITS1 phylogenetic trees. The Malaysian edible species
,
and
were grouped within Rhizostomeae, whereas other scyphozoans showed phylogenetic affinities to Semaestomeae and Kolpophorae.
and
appeared non-monophyletic; however their paraphyly was not confirmed. This study has provided the much needed baseline information on the taxonomy of Malaysian jellyfish species which have been substantiated by partial COI, 16S and ITS1 sequences. A total of 12 putative species of jellyfish were identified, which encompassed 12 genera.
While diversity studies and screening for enzyme activities are important elements of understanding fungal roles in the soil ecosystem, extracting and purifying the target enzyme from the fungal ...cellular system is also required to characterize the enzyme. This is, in particular, necessary before developing the enzyme for industrial-scale production. In the present study, partially purified α-amylase was obtained from strains of Pseudogymnoascus sp. obtained from Antarctic and Arctic locations. Partially purified α-amylases from these polar fungi exhibited very similar characteristics, including being active at 15 °C, although having a small difference in optimum pH. Both fungal taxa are good candidates for the potential application of cold-active enzymes in biotechnological industries, and further purification and characterization steps are now required. The α-amylases from polar fungi are attractive in terms of industrial development because they are active at lower temperatures and acidic pH, thus potentially creating energy and cost savings. Furthermore, they prevent the production of maltulose, which is an undesirable by-product often formed under alkaline conditions. Psychrophilic amylases from the polar Pseudogymnoascus sp. investigated in the present study could provide a valuable future contribution to biotechnological applications.
Patterns of modern human population structure are helpful in understanding the history of human migration and admixture. We conducted a study on genetic structure of the Malay population in Malaysia, ...using 54,794 genome-wide single nucleotide polymorphism genotype data generated in four Malay sub-ethnic groups in peninsular Malaysia (Melayu Kelantan, Melayu Minang, Melayu Jawa and Melayu Bugis). To the best of our knowledge this is the first study conducted on these four Malay sub-ethnic groups and the analysis of genotype data of these four groups were compiled together with 11 other populations' genotype data from Indonesia, China, India, Africa and indigenous populations in Peninsular Malaysia obtained from the Pan-Asian SNP database. The phylogeny of populations showed that all of the four Malay sub-ethnic groups are separated into at least three different clusters. The Melayu Jawa, Melayu Bugis and Melayu Minang have a very close genetic relationship with Indonesian populations indicating a common ancestral history, while the Melayu Kelantan formed a distinct group on the tree indicating that they are genetically different from the other Malay sub-ethnic groups. We have detected genetic structuring among the Malay populations and this could possibly be accounted for by their different historical origins. Our results provide information of the genetic differentiation between these populations and a valuable insight into the origins of the Malay sub-ethnic groups in Peninsular Malaysia.
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DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
The morphology of seven specimens of
Chrysaora chinensis
(Scyphomedusae, Semaestomae) obtained from four sites off the coast of Peninsular Malaysia was examined. Morphological characteristics of
C. ...chinensis
that encompasses structures such as the bell, tentacles, oral arms, stomach, manubrium, radial canals and gonads were described in detail. A total of 107 specimens that represented
C. chinensis
populations of four coastal areas of Peninsular Malaysia (East-Central, East-North, West-Central, and West-North) were also analysed for shape variation using geometric morphometric analysis. Procrustes superimposition, Principal Component Analysis (PCA) and Canonical Variate Analysis (CVA) were applied to the images of gastrovascular pouches of
C. chinensis
to extract the shape information. Independent contrasts were used for comparisons between shapes. There were no significant differences in shape variation between all the specimens based on the PCA results. However, CVA results showed shape variations between specimens taken from the four areas of Peninsular Malaysia, especially with higher magnitudes of Mahalanobis distances between the east and west coast areas, including between East-Central and East-North, but lower magnitudes were detected between the West-Central and West-North.
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EMUNI, FIS, FZAB, GEOZS, GIS, IJS, IMTLJ, KILJ, KISLJ, MFDPS, NLZOH, NUK, OBVAL, OILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
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