Aims
The study aimed for evaluate the efficacy of Pseudomonas knackmussiiMLR6 on growth promotion, photosynthetic responses, pigment contents and gene expression of the plant model Arabidopsis ...thaliana under NaCl stress.
Methods and Results
The strain MLR6 was isolated from the rhizopshere of the halophyte Salsola tetrandra collected from a natural saline Algerian soil. Results showed the ability of MLR6 to induce plant growth‐promoting traits even under NaCl stress. The inoculation with MLR6 improved the stomatal conductance, the transpiration rate, the total chlorophyll and carotenoid contents under salt stress. It conferred also an increase in fresh/dry weight as well as plant height. MLR6 inoculation further provided a positive effect on cell membrane stability by reducing the electrolyte leakage and priming the ROS accumulation after the salt exposition. Additionally, the expression of NHX1, HKT1, SOS2 and SOS3 as well as SAG13 and PR1 was maintained in MLR6‐bacterized plant at a similar level of controls.
Conclusions
The inoculation of A. thaliana with MLR6 improves plant growth and reduces damages caused by salt stress.
Significance and Impact of the Study
The use of P. knackmussiiMLR6 appears as a promising strategy to improve the sustainable agriculture under saline conditions.
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BFBNIB, FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SBCE, SBMB, UL, UM, UPUK
Anodic polarization of carbon steel electrodes in deaerated 0.01molL−1 NaCl+0.01molL−1 NaHCO3+0.001/0.01molL−1 Na2S solutions (with pH∼7.5) led to heterogeneous layers made of magnetite and ...mackinawite. A locally accelerated dissolution of the steel was observed in any case and the most corroded areas were covered with a layer enriched with mackinawite. This suggests that part of the electrode surface was blocked, covered with a magnetite rich layer where water reduction was favored. The effects of the galvanic coupling between a magnetite electrode and a bare carbon steel electrode covered with a ∼5mm thick argillites layer were then studied in a 0.01molL−1 NaCl+0.01molL−1 NaHCO3 solution at room temperature and 80°C. Electrochemical measurements demonstrated that a relatively high galvanic current initially flowed between the magnetite electrode (cathode) and the steel electrode (anode). However, this current decreased with time down to negligible values. Micro-Raman spectroscopy analysis revealed that a magnetite layer was formed on the steel electrode, thus suppressing the initial difference between anode and cathode. When the same experiment was performed with the steel electrode set under an argillites layer enriched with Na2S, the galvanic current density remained constant at a significant value (∼20μAcm−2) during the 11days of experiment. It led to the formation of a layer composed of mackinawite (and chukanovite) on the steel electrode. This result confirms that heterogeneous magnetite/mackinawite layers can favor galvanic corrosion even in anoxic conditions.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK, ZRSKP
Ionic magnetic fluid (ferrofluid) is a stable suspension of magnetic nanoparticles in water. Cobalt ferrite nanoparticles are interesting in view of high-density recording storage. The size of the ...magnetic particles strongly influences the physical properties of the ferrofluids. In this study, we describe the synthesis of ionic magnetic fluid in the presence of tartrate ions. By varying the amount of organic ligands, nanoparticles in a large range of size are obtained: the mean diameter varies from 3 to 10 nm. The effect of tartrate ions on the stability of the ionic magnetic fluid is also studied in relation with the size of the magnetic particles and the amount of adsorbed ligand.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK
Kawasaki disease (KD) is an acute vasculitis characterized by marked tropism of the coronary vessels. Usually a childhood disease, KD can occasionally be observed in adults. We report a case of ...adult-onset KD that presented as a prolonged fever of unknown origin with subsequent development of severe vasculitis, manifested by coronary aneurysms and peripheral gangrene of the lower limbs. Therapy with intravenous immunoglobulins, corticosteroids, aspirin, anticoagulants, and prostacyclin analogue resulted in rapid improvement in the patient's condition but he required partial distal amputation for irreversible gangrene. Peripheral ischaemia leading to gangrene is a very rare feature of KD vasculitis with only 19 cases previously published in the Medline-indexed literature. The outcomes of those patients were poor, being either fatal or requiring distal amputation. All prior cases affected young children less than 1 year old and, to the best of our knowledge, the present case is the first description of peripheral gangrene in adult-onset KD.
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DOBA, IJS, IZUM, KILJ, NUK, PILJ, PNG, SAZU, UILJ, UKNU, UL, UM, UPUK
Trivalent inorganic arsenic As(III) is an efficient anticancer agent used to treat patients suffering from acute promyelocytic leukemia. Recently, experimental studies have clearly demonstrated that ...this metalloid can also cure lymphoproliferative and/or pro-inflammatory syndromes in different murine models of chronic immune-mediated diseases. T helper (Th) 1 and Th17 lymphocytes play a central role in development of these diseases, in mice and humans, especially by secreting the potent pro-inflammatory cytokine interferon-γ and IL-17A, respectively. As(III) impairs basic functions of human T cells but its ability to modulate secretion of pro-inflammatory cytokines by differentiated Th lymphocytes is unknown. In the present study, we demonstrate that As(III), used at concentrations clinically achievable in plasma of patients, has no effect on the secretion of interferon-γ from Th1 cells but almost totally blocks the expression and the release of IL-17A from human Th17 lymphocytes co-stimulated for five days with anti-CD3 and anti-CD28 antibodies, in the presence of differentiating cytokines. In addition, As(III) specifically reduces mRNA levels of the retinoic-related orphan receptor (ROR)C gene which encodes RORγt, a key transcription factor controlling optimal IL-17 expression in fully differentiated Th17 cells. The metalloid also blocks initial expression of IL-17 gene induced by the co-stimulation, probably in part by impairing activation of the JNK/c-Jun pathway. In conclusion, our results demonstrate that As(III) represses expression of the major pro-inflammatory cytokine IL-17A produced by human Th17 lymphocytes, thus strengthening the idea that As(III) may be useful to treat inflammatory immune-mediated diseases in humans.
► Arsenic inhibits secretion of IL-17A from human naïve and memory Th17 lymphocytes. ► Arsenic represses early expression of IL-17A gene in human activated T lymphocytes. ► Arsenic interferes with activation of the JNK/c-Jun pathway in human T lymphocytes.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK
A 78-year-old woman developed an early knee-prosthesis infection due to multiresistant
Serratia marcescens that was successfully treated with high-dose meropenem, after failure of a long-term therapy ...combining imipenem and multiple surgical interventions. Because of its lower neurotoxicity, meropenem might be preferred to imipenem/cilastatin for the treatment of osteo-articular infections due to multiresistant Gram-negative bacilli in the elderly.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK