Background
Estimation of the depth of myometrial invasion (MI) in endometrial cancer is pivotal in the preoperatively staging. Magnetic resonance (MR) reports suffer from human subjectivity. ...Multiparametric MR imaging radiomics and parameters may improve the diagnostic accuracy.
Purpose
To discriminate between patients with MI ≥ 50% using a machine learning‐based model combining texture features and descriptors from preoperatively MR images.
Study Type
Retrospective.
Population
One hundred forty‐three women with endometrial cancer were included. The series was split into training (n = 107, 46 with MI ≥ 50%) and test (n = 36, 16 with MI ≥ 50%) cohorts.
Field Strength/Sequences
Fast spin echo T2‐weighted (T2W), diffusion‐weighted (DW), and T1‐weighted gradient echo dynamic contrast‐enhanced (DCE) sequences were obtained at 1.5 or 3 T magnets.
Assessment
Tumors were manually segmented slice‐by‐slice. Texture metrics were calculated from T2W and ADC map images. Also, the apparent diffusion coefficient (ADC), wash‐in slope, wash‐out slope, initial area under the curve at 60 sec and at 90 sec, initial slope, time to peak and peak amplitude maps from DCE sequences were obtained as parameters. MR diagnostic models using single‐sequence features and a combination of features and parameters from the three sequences were built to estimate MI using Adaboost methods. The pathological depth of MI was used as gold standard.
Statistical Test
Area under the receiver operating characteristic curve (AUROC), sensitivity, specificity, accuracy, positive predictive value, negative predictive value, precision and recall were computed to assess the Adaboost models performance.
Results
The diagnostic model based on the features and parameters combination showed the best performance to depict patient with MI ≥ 50% in the test cohort (accuracy = 86.1% and AUROC = 87.1%). The rest of diagnostic models showed a worse accuracy (accuracy = 41.67%–63.89% and AUROC = 41.43%–63.13%).
Data Conclusion
The model combining the texture features from T2W and ADC map images with the semi‐quantitative parameters from DW and DCE series allow the preoperative estimation of myometrial invasion.
Evidence Level
4
Technical Efficacy
Stage 3
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BFBNIB, FZAB, GIS, IJS, KILJ, NLZOH, NUK, OILJ, SBCE, SBMB, UL, UM, UPUK
Summary
Background
Histological evaluation of metabolic‐associated fatty liver disease (MAFLD) biopsies is subjective, descriptive and with interobserver variability.
Aims
To examine the relationship ...between different histological features (fibrosis, steatosis, inflammation and iron) measured with automated whole‐slide quantitative digital pathology and corresponding semiquantitative scoring systems, and the distribution of digital pathology measurements across Fatty Liver Inhibition of Progression (FLIP) algorithm and Steatosis, Activity and Fibrosis (SAF) scoring system
Methods
We prospectively included 136 consecutive patients who underwent liver biopsy for MAFLD at three Spanish centres (January 2017‐January 2020). Biopsies were scored by two blinded pathologists according to the Non‐alcoholic Steatohepatitis (NASH) Clinical Research Network system for fibrosis staging, the FLIP/SAF classification for steatosis and inflammation grading and Deugnier score for iron grading. Proportionate areas of collagen, fat, inflammatory cells and iron deposits were measured with computer‐assisted digital image analysis. A test‐retest experiment was performed for precision repeatability evaluation.
Results
Digital pathology showed strong correlation with fibrosis (r = 0.79; P < 0.001), steatosis (r = 0.85; P < 0.001) and iron (r = 0.70; P < 0.001). Performance was lower when assessing the degree of inflammation (r = 0.35; P < 0.001). NASH cases had a higher proportion of collagen and fat compared to non‐NASH cases (P < 0.005), whereas inflammation and iron quantification did not show significant differences between categories. Repeatability evaluation showed that all the coefficients of variation were ≤1.1% and all intraclass correlation coefficient values were ≥0.99, except those of collagen.
Conclusion
Digital pathology allows an automated, precise, objective and quantitative assessment of MAFLD histological features. Digital analysis measurements show good concordance with pathologists´ scores.
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BFBNIB, DOBA, FZAB, GIS, IJS, IZUM, KILJ, NLZOH, NUK, OILJ, PILJ, PNG, SAZU, SBCE, SBMB, SIK, UILJ, UKNU, UL, UM, UPUK
Background: Nutrient restriction is a common strategy to prevent metabolic disorders in fast growing broiler chickens, but limited information is available regarding the impact of diets with low ...protein and energy on leg abnormalities. Objective: Two experiments were conducted to evaluate the effect of varying crude protein (CP) and metabolizable energy (ME) levels on gastrocnemius tendon (GTeBS) and tibia breaking strength (TiBS), gait score (GS) and valgus/varus angulation (VAng). Methods: In Experiment 1, eight-d-old Ross 308 broilers (n=90) were randomly assigned into three treatments: 13/2,900 (13% CP and 2,900 kcal ME kg-1 of diet), 17/3,000 (17% CP and 3,000 kcal ME kg-1 of diet), and 21/3,025 or control (21% CP and 3,025 kcal ME kg-1 of diet). In Experiment 2, six-d-old Ross 308 chickens (n=192) were randomly distributed into two treatments: 16/3,000; 16% CP and 3,000 kcal ME kg-1 of diet and 21/3,000 or control; 21% CP and 3,000 kcal ME kg-1 of diet. In both experiments data were analyzed as one-way ANOVA. Results: In Experiment 1, broilers in the 17/3,000 and control treatments had similar (p>0.05) GTeBS (202 and 224 N, respectively), and TiBS (338 and 332 N, respectively). Birds in the 13/2,900 treatment showed higher GS (lower walking ability; 80% of birds with score >3), and greater VAng (53% of birds with score >2) than broilers in the control treatment (0% of birds with GS score >3 and 0% of birds with VAng score >2). Experiment 2, broilers in the 16/3,000 showed similar VAng, GS and TiBS than birds in the control treatment. However, birds in the 16/3,000 treatment showed lower (-26%) GTeBS than birds in the control treatment (p<0.05). Conclusions: Feeding broilers with 16% CP and 3,000 kcal ME kg-1 of diet did not affect tibia breaking strength, gait score and valgus/varus angulation. A diet containing 16% CP or less and 2,900-3,000 kcal ME kg-1 reduces tendon and tibia breaking strength.
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IZUM, KILJ, NUK, PILJ, PNG, SAZU, UL, UM, UPUK
Purpose
The aim of this study was to determine whether MRI texture analysis could predict the prognosis of patients with non-specific chronic low back pain.
Methods
A prospective observational study ...was conducted on 100 patients with non-specific chronic low back pain, who underwent a conventional MRI, followed by rehabilitation treatment, and revisited after 6 months. Sociodemographic variables, numeric pain scale (NPS) value, and the degree of disability as measured by the Roland–Morris disability questionnaire (RMDQ), were collected. The MRI analysis included segmentation of regions of interest (vertebral endplates and intervertebral disks from L3–L4 to L5–S1, paravertebral musculature at the L4–L5 space) to extract texture variables (PyRadiomics software). The classification random forest algorithm was applied to identify individuals who would improve less than 30% in the NPS or would score more than 4 in the RMDQ at the end of the follow-up. Sensitivity, specificity, and the area under the ROC curve were calculated.
Results
The final series included 94 patients. The predictive model for classifying patients whose pain did not improve by 30% or more offered a sensitivity of 0.86, specificity 0.57, and area under the ROC curve 0.71. The predictive model for classifying patients with a RMDQ score 4 or more offered a sensitivity of 0.83, specificity of 0.20, and area under the ROC curve of 0.52.
Conclusion
The texture analysis of lumbar MRI could help identify patients who are more likely to improve their non-specific chronic low back pain through rehabilitation programs, allowing a personalized therapeutic plan to be established.
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EMUNI, FIS, FZAB, GEOZS, GIS, IJS, IMTLJ, KILJ, KISLJ, MFDPS, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
Due to lack of native fluorescence, moxidectin (MOX) and abamectin (ABA) were derivatized varying the incubation time and the mass of derivatization reagents: trifluoroacetic anhydride (TFAA) and ...N-methylimidazole (NMI). The effect of those variables on the analytical signal of MOX and ABA was evaluated by spectrofluorometry. There are a few reports of the spectrofluorometric analysis of MOX and ABA. The typical experimental conditions of derivatization were not suitable for the analysis by spectrofluorometry. Therefore, it was necessary to explore the experimental conditions to achieve the analysis. The final conditions were NMI (30 μL), TFAA (45 μL) and incubation time of at least 60 min (λ
ex
= 390 nm, λ
em
= 450 nm). The analytical signals achieved with those conditions were more intense than those reported in other works. The conditions were used to analyze solvent standard solutions of MOX and ABA. Also, analyte spectra were obtained in extracts of bovine plasma and peach juice spiked with MOX and ABA, respectively. The derivatization works as pseudo-first-order reaction with rate constants of 0.000056 M
−1
s
−1
for MOX, and 0.000077 M
−1
s
−1
for ABA. Spectrofluorometry is easier and cheaper and is an alternative to chromatography to determine these drugs in different types of samples.
Graphical Abstract
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EMUNI, FIS, FZAB, GEOZS, GIS, IJS, IMTLJ, KILJ, KISLJ, MFDPS, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
Background: Active bone remodeling processes can be altered by the presence of reactive oxygen species and, therefore, cause leg problems in broiler chickens. The antioxidant activity present in ...grape seed extract (GSE) could be a viable alternative to high inclusion levels of vitamin E (VE) as a nutritional strategy to improve the antioxidant capacity of birds and, thus, prevent leg abnormalities. Objective: To evaluate the effect of partial substitution of VE with grape GSE on leg health and performance of broiler chickens. Methods: Four hundred and twenty newly-hatched Ross 308 male broiler chickens were distributed into three treatments: 1) Control-AL, a diet containing 40 IU/kg of VE and fed ad libitum (CAL); 2) Control-FR, the CAL diet but offered through a feed restriction program (CFR); and 3) grape seed extract-AL, a diet containing 10 mg/kg of GSE + 10 IU/kg of VE fed ad libitum (GSE-AL). The trial was conducted for 47 days. Feed intake and body weight of the chickens were recorded weekly to evaluate performance. At day 43, gait score (GS), valgus/varus angulation (AngV), foot burn (FB), and hock burn (HB) lesions were evaluated. At day 47, tibia-breaking strength (TBS) and gastrocnemius tendon-breaking strength (GTBS) were evaluated. Results: Birds in the GSE-AL treatment showed reduced (p≤0.05) GS compared to the other treatments. No statistical differences (p>0.05) were found in AngV, HB, FB, TBS, TGBS, and growth performance among treatments. Conclusions: These results suggest that GSE may partially replace VE in broiler diets without negative effects on growth performance or leg health. Further research is required to evaluate the potential of grape seed extract to replace VE or other ingredients with antioxidant activity under different rearing conditions and feeding programs.
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IZUM, KILJ, NUK, PILJ, PNG, SAZU, UL, UM, UPUK
Grafting polyethylene glycol (PEG) onto a polymer's surface is widely used to improve biocompatibility by reducing protein and cell adhesion. Although PEG is considered to be bioinert, its ...incorporation onto biomaterials has shown to improve cell viability depending on the amount and molecular weight (MW) used. This phenomenon was studied here by grafting PEG of three MW onto polyurethane (PU) substrata at three molar concentrations to assess their effect on PU surface properties and on the viability of osteoblasts and fibroblasts. PEG formed a covering on the substrata which increased the hydrophilicity and surface energy of PUs. Among the results, it was observed that osteoblast viability increased for all MW and grafting densities of PEG employed compared with unmodified PU. However, fibroblast viability only increased at certain combinations of MW and grafting densities of PEG, suggesting an optimal level of these parameters. PEG grafting also promoted a more spread cell morphology than that exhibited by unmodified PU; nevertheless, cells became apoptotic-like as PEG MW and grafting density were increased. These effects on cells could be due to PEG affecting culture medium pH, which became more alkaline at higher MW and concentrations of PEG. Results support the hypothesis that surface energy of PU substrates can be tuned by controlling the MW and grafting density of PEG, but these parameters should be optimized to promote cell viability without inducing apoptotic-like behavior.
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IZUM, KILJ, NUK, PILJ, PNG, SAZU, UL, UM, UPUK
The main cause of flesh browning in ‘Rojo Brillante’ persimmon fruit is mechanical damage caused during harvesting and packing. Innovation and research on nondestructive techniques to detect this ...phenomenon in the packing lines are necessary because this type of alteration is often only seen when the final consumer peels the fruit. In this work, we have studied the application of hyperspectral imaging in the range of 450–1040 nm to detect mechanical damage without any external symptoms. The fruit was damaged in a controlled manner. Later, images were acquired before and at 0, 1, 2 and 3 days after damage induction. First, the spectral data captured from the images were analysed through an algorithm based on principal component analysis (PCA). The aim was to automatically separate intact and damaged fruit, and to detect the damage in the PC images when present. With this algorithm, 90.0% of intact fruit and 90.8% of damaged fruit were correctly detected. A model based on partial least squares—discriminant analysis (PLS-DA), was later calibrated using the mean spectrum of the pixels detected as damaged, to determine the moment when the fruit was damaged. The model differentiated fruit corresponding correctly to 0, 1, 2 and 3 days after damage induction, achieving a total accuracy of 99.4%.
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