The mitophagy receptor Nix interacts with LC3/GABARAP proteins, targeting mitochondria into autophagosomes for degradation. Here we present evidence for phosphorylation-driven regulation of the ...Nix:LC3B interaction. Isothermal titration calorimetry and NMR indicate a ~100 fold enhanced affinity of the serine 34/35-phosphorylated Nix LC3-interacting region (LIR) to LC3B and formation of a very rigid complex compared to the non-phosphorylated sequence. Moreover, the crystal structure of LC3B in complex with the Nix LIR peptide containing glutamic acids as phosphomimetic residues and NMR experiments revealed that LIR phosphorylation stabilizes the Nix:LC3B complex via formation of two additional hydrogen bonds between phosphorylated serines of Nix LIR and Arg11, Lys49 and Lys51 in LC3B. Substitution of Lys51 to Ala in LC3B abrogates binding of a phosphomimetic Nix mutant. Functionally, serine 34/35 phosphorylation enhances autophagosome recruitment to mitochondria in HeLa cells. Together, this study provides cellular, biochemical and biophysical evidence that phosphorylation of the LIR domain of Nix enhances mitophagy receptor engagement.
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IZUM, KILJ, NUK, PILJ, PNG, SAZU, UL, UM, UPUK
This study evaluates the effects of seasonal conditions and exposure periods on trace element concentrations in samples of the epiphytic moss
Pylaisia polyantha
when transplanted into urban areas. ...This assessment was carried out in summer and winter at four sites differing in their level of technogenic trace element load. The contents of 25 trace elements (As, Ba, Br, Ca, Ce, Co, Cr, Cs, Eu, Fe, Hf, La, Lu, Mo, Nd, Rb, Sb, Sc, Sm, Sr, Tb, Th, U, Yb, and Zn) were determined using neutron-activation analysis, and it was shown that seasonal conditions do not affect vital activity in
P. polyantha
graft moss. For most elements, the greatest increase in trace element concentration in
P. polyantha
transplant moss was observed within one month. The high sensitivity of this epiphytic moss-transplant to the level of technogenic load has thus been demonstrated, and it may find utility in future research with similar objectives.
The covalent conjugation of ubiquitin-fold modifier 1 (UFM1) to proteins generates a signal that regulates transcription, response to cell stress, and differentiation. Ufmylation is initiated by ...ubiquitin-like modifier activating enzyme 5 (UBA5), which activates and transfers UFM1 to ubiquitin-fold modifier-conjugating enzyme 1 (UFC1). The details of the interaction between UFM1 and UBA5 required for UFM1 activation and its downstream transfer are however unclear. In this study, we described and characterized a combined linear LC3-interacting region/UFM1-interacting motif (LIR/UFIM) within the C terminus of UBA5. This single motif ensures that UBA5 binds both UFM1 and light chain 3/γ-aminobutyric acid receptor-associated proteins (LC3/GABARAP), two ubiquitin (Ub)-like proteins. We demonstrated that LIR/UFIM is required for the full biological activity of UBA5 and for the effective transfer of UFM1 onto UFC1 and a downstream protein substrate both in vitro and in cells. Taken together, our study provides important structural and functional insights into the interaction between UBA5 and Ub-like modifiers, improving the understanding of the biology of the ufmylation pathway.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Ubiquitin fold modifier 1 (UFM1) is a member of the ubiquitin-like protein family. UFM1 undergoes a cascade of enzymatic reactions including activation by UBA5 (E1), transfer to UFC1 (E2) and ...selective conjugation to a number of target proteins via UFL1 (E3) enzymes. Despite the importance of ufmylation in a variety of cellular processes and its role in the pathogenicity of many human diseases, the molecular mechanisms of the ufmylation cascade remains unclear. In this study we focused on the biophysical and biochemical characterization of the interaction between UBA5 and UFC1. We explored the hypothesis that the unstructured C-terminal region of UBA5 serves as a regulatory region, controlling cellular localization of the elements of the ufmylation cascade and effective interaction between them. We found that the last 20 residues in UBA5 are pivotal for binding to UFC1 and can accelerate the transfer of UFM1 to UFC1. We solved the structure of a complex of UFC1 and a peptide spanning the last 20 residues of UBA5 by NMR spectroscopy. This structure in combination with additional NMR titration and isothermal titration calorimetry experiments revealed the mechanism of interaction and confirmed the importance of the C-terminal unstructured region in UBA5 for the ufmylation cascade.
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IZUM, KILJ, NUK, PILJ, PNG, SAZU, UL, UM, UPUK
The paper analyses the results of active biomonitoring of atmospheric air pollution in the mining town of the Kuzbass region by the most toxic metals. The epiphytic moss Pylaisia polyantha (Hedw.) B. ...S. G. was used as a bioindicators; determination of metal content in moss samples was carried out using neutron activation analysis and atomic emission spectrometry methods. Based on the results of the study, spatial distributions for pollution levels of the most toxic metals were plotted and their sources were identified.
Context
Agricultural land abandonment across the steppe belt of Eurasia has provided an opportunity for the restoration of steppe landscapes in recent decades. However, global food demands are about ...to revert this trajectory and put restored steppe landscapes at risk.
Objectives
We analysed steppe development in southern Russia in the last 40 years, assessed its spatial patterns and drivers of change for several periods.
Methods
Using Landsat imagery, we mapped the permanent steppe and steppe restoration from 1990 to 2018. Based on regression tree models, we evaluate and explain its dynamics. Results were compared with district-level trends in land-use intensities of cropland.
Results
We found 70% of the steppe in 2018 represented permanent steppe and 30% of former cropland dominantly abandoned in the postsocialism (1990–2000). The permanent steppe and steppe restored in the postsocialism (1990–2000) were located far from settlements, on rough terrain and in districts of the Virgin Land Campaign (1954–1963). In recent decades, the patterns of steppe restoration (2000–2018) were mostly determined by unfavourable agroclimatic conditions and distance from grain storage facilities. The restoration pattern reflects regional differences in land-use intensities, e.g., isolated steppe patches mostly appeared in areas of intensive agricultural land-use.
Conclusions
Steppe restoration has appeared in areas marginal for agricultural production, with poor natural conditions and little human footprint. Consequently, the permanent steppe became less fragmented and a more continuous steppe landscape resulted. The remaining isolated steppe patches require attention in restoration programs as they are mostly located in areas of intensive agricultural land-use.
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EMUNI, FZAB, GEOZS, IJS, IMTLJ, KILJ, KISLJ, MFDPS, NUK, OILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
The work presents the results of a study of atmospheric air pollution with heavy metals in a small mining town in the Kemerovo region. The assessment was carried out using the active biomonitoring ...method. The epiphytic moss
Pylaisiella polyantha
served as a bioindicator. The content of chemical elements was determined using the neutron activation method. Elements and areas of the city were identified for which high levels of concentrations values were obtained relative to background.
Nowadays consumers can express their identities not only through their possessions and buying behavior, but also using social media and digital networks. This article aims to understand these digital ...consumer behaviors by focusing on identity strength and the identity signaling phenomenon. We develop a conceptual model that combines internal and external factors to explain the intensity and content of digital identity-related behaviors. We use the example of gender identity to build our research propositions, as gender is one of the most frequently and intensely debated identities in online consumer discussions. Further, we propose how digital and offline identity signaling behaviors are intertwined, and discuss the online behaviors of trans consumers. In doing so, our conceptual work highlights the unique features of digital identity signaling behaviors as well as the complexity of identities, including gender, and provides useful insights for researchers and marketers.
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CEKLJ, EMUNI, FIS, FZAB, GEOZS, GIS, IJS, IMTLJ, KILJ, KISLJ, MFDPS, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
The Rcs-signaling system is one of the most remarkable phosphorelay pathways in
Enterobacteriaceae, comprising several membrane-bound and soluble proteins. Within the complex phosphotransfer pathway, ...the histidine phosphotransferase (HPt) domain of the RcsD membrane-bound component serves as a crucial factor in modulating the phosphorylation state of the transcription factor RcsB. We have identified a new domain, RcsD-ABL, located N terminally to RcsD-HPt that interacts with RcsB as well. We have determined its structure, characterized its interaction interface with RcsB, and built a structural model of the complex of the RcsD-ABL domain with RcsB. Our results indicate that the effector domain of RcsB, which normally binds to DNA, is recognized by RcsD-ABL, whereas the HPt domain interacts with the phosphoreceiver domain of RcsB.
► NMR structure of a newly identified domain within RcsD named RcsD-ABL ► RcsD-ABL functions as a binding domain for the response regulator RcsB ► Mapping the interface of the protein complex RcsD-ABL:RcsB ► Newly identified domain stabilizes binding of RcsB to RcsD-HPt
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
Consumers' compliance with recommended behavior during the COVID-19 pandemic helps contain the spread of the virus and positively impacts marketplace outcomes. This study investigates the effect of ...consumers' tolerance of uncertainty on COVID-19 concern, compliance with recommended behavior, and panic buying intentions, across four countries (Germany and USA with a low power distance index; India and The Philippines with a high power distance index; N = 1272). We test the moderating role of power distance, mindfulness, and trust in scientists, among these relationships. Our results show that tolerance of uncertainty is negatively associated with COVID-19 concern, and COVID-19 concern is positively associated with compliance and panic buying intentions. In high power distance countries, tolerance of uncertainty is negatively associated with compliance. The negative association of tolerance of uncertainty with COVID-19 concern is more pronounced at low levels of mindfulness, and consumers with high COVID-19 concern and high trust in scientists demonstrated the highest compliance. Our findings reveal that stressing the importance of mindfulness, though positive overall, might not yield more compliance. Interventions to make consumers more concerned about the consequences of the pandemic and, at the same time, enhancing their trust in scientists, can lead to higher levels of compliance.
•Tolerance of uncertainty (TU) negatively affects consumers’ COVID concern.•This effect is more pronounced at low levels of mindfulness.•COVID concern positively affects consumers’ compliance with recommended behavior.•Those with higher COVID concern and higher trust in scientists show more compliance.•In high power distance cultures, TU negatively affects consumers’ compliance.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
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