Atrial fibrillation (AF) is the most common arrhythmia worldwide, affecting 1% of the population over 60 years old. The incidence and prevalence of AF are increasing globally, representing a relevant ...health problem, suggesting that more advanced strategies for predicting risk stage are highly needed. miRNAs mediate several processes involved in AF. Our aim was to identify miRNAs with a prognostic value as biomarkers in patients referred for AF ablation and its association with LVA extent, based on low-voltage area (LVA) maps. In this study, we recruited 44 AF patients referred for catheter ablation. We measured the expression of 84 miRNAs in plasma from peripheral blood in 3 different groups based on LVA extent. Expression analysis showed that miR-486-5p was significantly increased in patients with broader LVA (4-fold, p = 0.0002; 5-fold, p = 0.0001). Receiver operating characteristic curve analysis showed that miR-486-5p expression could predict atrium LVA (AUC, 0.8958; p = 0.0015). Also, miR-486-5p plasma levels were associated with AF-type (AUC, 0.7137; p = 0.0453). In addition, miR-486-5p expression was positively correlated with LVA percentage, left atrial (LA) area, and LA volume (r = 0.322, p = 0.037; r = 0.372, p = 0.015; r = 0.319, p = 0.045, respectively). These findings suggest that miR-486-5p expression might have prognostic significance in LVA extent in patients with AF.
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IZUM, KILJ, NUK, PILJ, PNG, SAZU, UL, UM, UPUK
A set of ensemble integrations from the Coupled Model Intercomparison Project phase 5, with historical forcing plus RCP4.5 scenario, are used to explore if state-of-the-art climate models are able to ...simulate previously reported linkages between sea-ice concentration (SIC) anomalies over the eastern Arctic, namely in the Greenland–Barents–Kara Seas, and lagged atmospheric circulation that projects on the North Atlantic Oscillation (NAO)/Arctic Oscillation (AO). The study is focused on variability around the long-term trends, so that all anomalies are detrended prior to analysis; the period of study is 1979–2013. The model linkages are detected by applying maximum covariance analysis. As also found in observational data, all the models considered here show a statistically significant link with sea-ice reduction over the eastern Arctic followed by a negative NAO/AO-like pattern. If the simulated relationship is found at a lag of one month, the results suggest that a stratospheric pathway could be at play as the driving mechanism; in observations this is preferentially shown for SIC in November. The interference of a wave-like anomaly over Eurasia, accompanying SIC changes, with the climatological wave pattern appears to be key in setting the mediating role of the stratosphere. On the other hand, if the simulated relationship is found at a lag of two months, the results suggest that tropospheric dynamics are dominant, presumably due to transient eddy feedback; in observations this is preferentially shown for SIC in December. The results shown here and previous evidence from atmosphere-only experiments emphasize that there could be a detectable influence of eastern Arctic SIC variability on mid-latitude atmospheric circulation anomalies. Even if the mechanisms are robust among the models, the timing of the simulated linkages strongly depends on the model and does not generally mimic the observational ones. This implies that the atmospheric sensitivity to sea-ice changes largely depends on the mean-flow and parameterizations, which could lead to misleading conclusions elsewhere if a multi-model ensemble-mean approach is adopted. It might also represent an important source of uncertainty in climate prediction and projection. Modelling efforts are hence further required to improve representation of the background atmospheric circulation and reduce biases, in order to attain more accurate covariability.
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DOBA, EMUNI, FIS, FZAB, GEOZS, GIS, IJS, IMTLJ, IZUM, KILJ, KISLJ, MFDPS, NLZOH, NUK, OBVAL, OILJ, PILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, SIK, UILJ, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
•Tillage reduction was effective for maize cultivation in irrigated conditions.•Traditional N fertilizer rates could be reduced half without yield loss.•The reduction of tillage and medium N rates ...led to a greater water and N use efficiency.
Mediterranean rainfed areas are transformed into irrigation to stabilize or increase crop yields. The gradual occupation of irrigation leads to an increase in nitrogen use and intensity of tillage. The aim of this work was to evaluate the combined impact of tillage systems and mineral N fertilization rates on maize grain yield, water and nitrogen use efficiencies (WUE and NUE) under Mediterranean irrigated conditions. The study was carried out in NE Spain during three maize growing seasons (i.e. years 2015, 2016 and 2017). A long-term (LTE) tillage and N rate field experiment established in 1996 under rainfed conditions was transformed into irrigation with maize (Zea mays L.) monocrop as cropping system in 2015. Three types of tillage (conventional tillage, CT; reduced tillage, RT; no-tillage, NT) and three mineral N fertilization rates (0, 200, 400 kg N ha−1) were compared. In 2015, an adjacent experiment (short-term experiment, STE) with the same layout was set up in an area previously managed under long-term rainfed NT for the last 21 years. In the long-term tillage and N fertilization combination (LTE), the reduction of tillage (NT and RT) led to greater grain yield when applying 200 and 400 kg N ha−1 compared to the use of the same rates under CT. Differently, in the sort-term experiment with preceding NT (STE), tillage systems did not influence grain yields, while N application led to greater yields than the control (0 kg N ha−1). In both situations (LTE and STE), NT and RT enhanced soil water content before planting leading to greater crop growth compared to CT. The lack of available water under CT caused lower maize stover, yield, and yield components in LTE and, therefore, lower WUEB (for biomass) and WUEY (for yield). In LTE, the use of long-term CT led to a significant accumulation of nitrate compared to NT. Differently, in the STE, soil nitrate content did not show differences between tillage systems. In the LTE, water and N were used more efficiently to produce aboveground biomass and grain yield in RT and NT. Our study shows that in Mediterranean agroecosystems transformed into irrigation the use of NT and RT with medium rates of N leads to greater maize yield, WUE and NUE than the traditional management based on CT with high rates of mineral N. In rainfed areas with long-term history of no-till, this soil management system can be successfully maintained if transformed into irrigation.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
A two-phase fermentation process for the treatment of waste, intended for the recovery of hydrogen for energy use, was investigated in its initial fermentation phase. Hydrogen production was obtained ...from a mixed culture based on an active mesophilic inoculum without any selective treatment being applied. The liquid stream generated by the hydrogen fermentation process was stabilized in the following, methanogenic, phase for the recovery of methane and further breaking down of the waste stream. The whole process was carried out at a temperature in the mesophilic range (34
°C). The substrate used was an unsterilized mixture of the organic fraction of municipal solid wastes (OFMSW) and slaughterhouse waste from a poultry-processing plant. The hydrogen-producing phase was capable of stable performance under the hydraulic retention times (HRTs) evaluated (3 and 5 days). No methane was detected in the first phase at any point during the whole period of the experiment and the hydrogen yield showed no symptoms of declining as time elapsed. The amount of hydrogen obtained from the fermentation process was in the range of 52.5–71.3
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK
Neutron dose coefficients for standard irradiation geometries have been reported in International Commission on Radiological Protection (ICRP) Publication 116 for the ICRP Publication 110 adult ...reference phantoms. In the present work, organ and effective dose coefficients have been calculated for a receptor in both upright and articulated (bent) postures representing more realistic working postures exposed to a mono-energetic neutron radiation field. This work builds upon prior work by Dewji and co-workers comparing upright and bent postures for exposure to mono-energetic photon fields. Simulations were conducted using the Oak Ridge National Laboratory’s articulated stylized adult phantom, “Phantom wIth Moving Arms and Legs” (PIMAL) software package, and the Monte Carlo N-Particle (MCNP) version 6.1.1 radiation transport code. Organ doses were compared for the upright and bent (45° and 90°) phantom postures for neutron energies ranging from 1 × 10
− 9
to 20 MeV for the ICRP Publication 116 external exposure geometries—antero-posterior (AP), postero-anterior (PA), and left and right lateral (LLAT, RLAT). Using both male and female phantoms, effective dose coefficients were computed using ICRP Publication 103 methodology. The resulting coefficients for articulated phantoms were compared to those of the upright phantom. Computed organ and effective dose coefficients are discussed as a function of neutron energy, phantom posture, and source irradiation geometry. For example, it is shown here that for the AP and PA irradiation geometries, the differences in the organ coefficients between the upright and bent posture become more pronounced with increasing bending angle. In the AP geometry, the brain dose coefficients are expectedly higher in the bent postures than in the upright posture, while all other organs have lower dose coefficients, with the thyroid showing the greatest difference. Overall, the effective dose estimated for the upright phantom is more conservative than that for the articulated phantom, which may have ramifications in the estimation or reconstruction of radiation doses.
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EMUNI, FIS, FZAB, GEOZS, GIS, IJS, IMTLJ, KILJ, KISLJ, MFDPS, NLZOH, NUK, OBVAL, OILJ, PNG, SAZU, SBCE, SBJE, SBMB, SBNM, UKNU, UL, UM, UPUK, VKSCE, ZAGLJ
Staphylococcus aureus is one of the pathogens most frequently isolated from cases of mastitis worldwide. To decrease the effect of S. aureus mastitis in dairy farming, alternative strategies for ...controlling mastitis are needed that depend on a better knowledge of cow-to-cow variations in S. aureus antibody production. The present study sought to explore the diversity of S. aureus antibodies produced by dairy cows with a distinct mastitis history and vaccinated with a polyvalent mastitis vaccine. We obtained protein extracts from S. aureus isolates derived from persistent subclinical mastitis. Proteins were fractionated using 2-dimensional gel electrophoresis and Western blotting. Then, Western blotting membranes were exposed to sera from 24 dairy cows that had been divided into the following groups: vaccinated dairy cows that were infected with S. aureus, further subdivided according to whether they (a) remained infected by S. aureus or (b) recovered from the intramammary infection; unvaccinated dairy cows infected with S. aureus; and vaccinated healthy dairy cows with no history of S. aureus mastitis. Proteins found to be reactive by Western blot were identified by mass spectrometry (MALDI/TOF-TOF). Our most important finding was that F0F1 ATP synthase subunit α, succinyl-diaminopimelate desuccinylase, and cysteinyl-tRNA synthetase were potential candidate proteins for the prevention of S. aureus mastitis. This study strengthens the notion that variations among animals should not be ignored and shows that the heterogeneity of antibody production against anti-staphylococcal antigens in animals may enable the identification of new immunotherapy targets.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
The hermaphrodite Pacific oyster Crassostrea gigas displays a high energy allocation to reproduction. We studied the expression of AMP-activated protein kinase (AMPK) during gametogenesis in the ...gonad and characterized the mRNA sequences of the AMPK subunits: the AMPK alpha mRNA sequence was previously characterized; we identified AMPK beta, AMPK gamma, and mRNAs of putative AMPK-related targets following bioinformatics mining on existing genomic resources. We analyzed the mRNA expression of the AMPK alpha, beta, and gamma subunits in the gonads of male and female oysters through a reproductive cycle, and we quantified the mRNA expression of genes belonging to fatty acid and glucose metabolism. AMPK alpha mRNA levels were more abundant in males at the first stage of gametogenesis, when mitotic activity and the differentiation of germinal cells occur, and were always more abundant in males than in females. Some targets of fatty acid and glucose metabolism appeared to be correlated with the expression of AMPK subunits at the mRNA level. We then analyzed the sex-specific AMPK activity by measuring the phosphorylation of the catalytic AMPK alpha protein and its expression at the protein level. Both the amount of AMPK alpha protein and threonine 172 phosphorylation appeared to be almost totally inhibited in mature female gonads at stage 3, at the time when accumulation of reserves in oocytes was promoted, while it remained at a high level in mature spermatozoa. Its activation might play a sex-dependent role in the management of energy during gametogenesis in oyster.
Transcriptional regulation of ribosome and tRNA synthesis plays a central role in determining protein synthetic capacity and is tightly controlled in response to nutrient availability and cellular ...stress. In Saccharomyces cerevisiae, the regulation of ribosome and tRNA synthesis was recently shown to involve the Cdc-like kinase Kns1 and the GSK-3 kinase Mck1. In this study, we explored additional roles for these conserved kinases in processes connected to the target of rapamycin complex 1 (TORC1). We conducted a synthetic chemical-genetic screen in a kns1Δ mck1Δ strain and identified many novel rapamycin-hypersensitive genes. Gene ontology analysis showed enrichment for TORC1-regulated processes (vesicle-mediated transport, autophagy, and regulation of cell size) and identified new connections to protein complexes including the protein kinase CK2. CK2 is considered to be a constitutively active kinase and in budding yeast, the holoenzyme comprises two regulatory subunits, Ckb1 and Ckb2, and two catalytic subunits, Cka1 and Cka2. We show that Ckb1 is differentially phosphorylated in vivo and that Kns1 mediates this phosphorylation when nutrients are limiting and under all tested stress conditions. We determined that the phosphorylation of Ckb1 does not detectably affect the stability of the CK2 holoenzyme but correlates with the reduced occupancy of Ckb1 on tRNA genes after rapamycin treatment. Thus, the differential occupancy of tRNA genes by CK2 is likely to modulate its activation of RNA polymerase III transcription. Our data suggest that TORC1, via its effector kinase Kns1, may regulate the association of CK2 with some of its substrates by phosphorylating Ckb1.
Background: Protein kinase CK2 participates in many regulated processes but the regulation of CK2 is enigmatic.
Results: A regulatory subunit of CK2 is phosphorylated under multiple stress conditions by the target of rapamycin complex 1 (TORC1) effector kinase Kns1.
Conclusion: CK2 is a downstream target of TORC1 signaling.
Significance: TOR activity via Kns1 may regulate CK2 function by affecting the interaction of the CK2 holoenzyme with its substrates.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
To evaluate the clinically relevant abnormalities as visualized on CT and MR imaging in children with symmetric and asymmetric bilateral sensorineural hearing loss (SNHL), in relation to age and the ...severity of hearing loss.
Retrospective cohort study.
Tertiary referral otology and audiology center.
From January 2006 until January 2016, a total of 207 children diagnosed with symmetric and asymmetric bilateral SNHL were included. They underwent CT and/or MR imaging for the evaluation of the etiology of their hearing loss.
Radiologic abnormalities associated with SNHL.
302 scans were performed in 207 children (median age of 0.8 years old) with bilateral SNHL. The most frequently identified cause of bilateral SNHL was a malformation of the labyrinth. The combined diagnostic yield of CT and MR imaging was 32%. The diagnostic yield of MR (34%) was considerably higher than that of CT (20%). We found a higher rate of abnormalities in children with profound hearing loss (41%) compared to milder hearing loss (8–29%), and in asymmetric SNHL (52%) compared to symmetric SNHL (30%).
Imaging is essential in the etiologic evaluation of children with bilateral SNHL. The highest diagnostic yield is found in children with bilateral asymmetric SNHL or profound SNHL. Based on our findings, MR is the primary imaging modality of choice in the etiological evaluation of children with bilateral SNHL because of its high diagnostic yield.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UL, UM, UPCLJ, UPUK, ZRSKP
The greenhouse gases and black carbon emission inventory from IPCC key category Energy was accomplished for the Mezquital Valley, one of the most polluted regions in Mexico, as the Mexico City ...wastewater have been continuously used in agricultural irrigation for more than a hundred years. In addition, thermoelectric, refinery, cement and chemistry industries are concentrated in the southern part of the valley, near Mexico City. Several studies have reported air, soil, and water pollution data and its main sources for the region. Paradoxically, these sources contaminate the valley, but boosted its economic development. Nevertheless, no research has been done concerning GHG emissions, or climate change assessment. This paper reports inventories performed by the 1996 IPCC methodology for the baseline year 2005. Fuel consumption data were derived from priority sectors such as electricity generation, refineries, manufacturing & cement industries, transportation, and residential use. The total CO2 emission result was 13,894.9Gg, which constituted three-quarters of Hidalgo statewide energy category. The principal CO2 sources were energy transformation (69%) and manufacturing (19%). Total black carbon emissions were estimated by a bottom-up method at 0.66Gg. The principal contributor was on-road transportation (37%), followed by firewood residential consumption (26%) and cocked brick manufactures (22%). Non-CO2 gas emissions were also significant, particularly SO2 (255.9Gg), which accounts for 80% of the whole Hidalgo State emissions. Results demonstrated the negative environmental impact on Mezquital Valley, caused by its role as a Megacity secondary fuel and electricity provider, as well as by the presence of several cement industries.
•First GHG & black carbon inventory for Mezquital Valley: Mexico City energy supplier•Energy industries caused the largest CO2 and SO2 emissions from residual fuel oil.•Diesel transportation was the main black carbon source.•Mezquital Valley has the main GHG emissions' sources in the state of Hidalgo: 75%.
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GEOZS, IJS, IMTLJ, KILJ, KISLJ, NLZOH, NUK, OILJ, PNG, SAZU, SBCE, SBJE, UILJ, UL, UM, UPCLJ, UPUK, ZAGLJ, ZRSKP
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